Küffer, Stefan

[["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Cancer Research"],["dc.bibliographiccitation.volume","74"],["dc.contributor.author","Bohnenberger, Hanibal"],["dc.contributor.author","Stroebel, Philipp"],["dc.contributor.author","Henric-Petri, Hannah"],["dc.contributor.author","Lenz, Christof"],["dc.contributor.author","Emmert, Alexander"],["dc.contributor.author","Bremmer, Felix"],["dc.contributor.author","Strecker, Jasmin"],["dc.contributor.author","Holland, Rainer"],["dc.contributor.author","Hinterthaner, Marc"],["dc.contributor.author","Corso, Jasmin"],["dc.contributor.author","Wagner, Sebastian"],["dc.contributor.author","Kueffer, Stefan"],["dc.contributor.author","Sebastian, Martin"],["dc.contributor.author","Bergmann, Lothar"],["dc.contributor.author","Danner, Bernd"],["dc.contributor.author","Schoendube, Friedrich Albert"],["dc.contributor.author","Serve, Hubert"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Oellerich, Thomas"],["dc.date.accessioned","2018-11-07T09:33:49Z"],["dc.date.available","2018-11-07T09:33:49Z"],["dc.date.issued","2014"],["dc.identifier.doi","10.1158/1538-7445.AM2014-2487"],["dc.identifier.isi","000349906903219"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32050"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Assoc Cancer Research"],["dc.publisher.place","Philadelphia"],["dc.relation.conference","105th Annual Meeting of the American-Association-for-Cancer-Research (AACR)"],["dc.relation.eventlocation","San Diego, CA"],["dc.relation.issn","1538-7445"],["dc.relation.issn","0008-5472"],["dc.title","Comprehensive quantitative proteomic profiling of lung cancers reveals novel biomarkers and potential drug targets"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2523"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Translational Lung Cancer Research"],["dc.bibliographiccitation.lastpage","2538"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Yao, Sha"],["dc.contributor.author","Peng, Luogen"],["dc.contributor.author","Elakad, Omar"],["dc.contributor.author","Küffer, Stefan"],["dc.contributor.author","Hinterthaner, Marc"],["dc.contributor.author","Danner, Bernhard C."],["dc.contributor.author","Hammerstein-Equord, Alexander von"],["dc.contributor.author","Ströbel, Philipp"],["dc.contributor.author","Bohnenberger, Hanibal"],["dc.date.accessioned","2021-08-12T07:45:40Z"],["dc.date.available","2021-08-12T07:45:40Z"],["dc.date.issued","2021"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.21037/tlcr-20-1039"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88522"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation.eissn","2226-4477"],["dc.relation.issn","2218-6751"],["dc.relation.orgunit","Institut für Pathologie"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","One carbon metabolism in human lung cancer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","704"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Histopathology"],["dc.bibliographiccitation.lastpage","710"],["dc.bibliographiccitation.volume","70"],["dc.contributor.author","Buerger, Tobias"],["dc.contributor.author","Schaefer, Inga-Marie"],["dc.contributor.author","Kueffer, Stefan"],["dc.contributor.author","Bohnenberger, Hanibal"],["dc.contributor.author","Reuter-Jessen, Kirsten"],["dc.contributor.author","Chan, John Kwok-Cheung"],["dc.contributor.author","Emmert, Alexander"],["dc.contributor.author","Hinterthaner, Marc"],["dc.contributor.author","Marx, Alexander"],["dc.contributor.author","Stroebel, Philipp"],["dc.date.accessioned","2018-11-07T10:25:44Z"],["dc.date.available","2018-11-07T10:25:44Z"],["dc.date.issued","2017"],["dc.description.abstract","AimsThe vast majority of type A thymomas are diagnosed in tumour stages 1 or 2, and metastatic cases are exceedingly rare. The histological and genetic features of such metastatic type A thymomas have not been described in detail. Methods and resultsFive metastatic type A thymomas in tumour stage Masaoka IVb that had been reviewed by a panel of expert pathologists were analysed using comparative genomic hybridization (CGH). Cases 1, 2 and 3 showed the prototypical morphology of type A thymomas with mainly solid growth patterns. These cases displayed only very subtle nuclear irregularities and slight nuclear crowding, but no other atypical features. Mitoses were absent. Cases 3 and 4, in contrast, had a distinctly atypical morphology. CGH revealed partially recurrent alterations in four cases (with and without atypical morphology), including gains on chromosome 1q (one case), 17q (two cases), chromosome 19 (three cases) and 22q (one case) and losses on chromosome 17p (two cases) and 22q (one case). ConclusionRare metastatic type A thymomas, both with typical and atypical' histological features, show partially recurrent genomic alterations that differ from the much more frequent localized and indolent tumours. The fact that these alterations were recurring points to a link between clinical behaviour and molecular features. Our findings may have implications for the management and treatment of such tumours."],["dc.identifier.doi","10.1111/his.13138"],["dc.identifier.isi","000397588600003"],["dc.identifier.pmid","27926794"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42916"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Wiley"],["dc.relation.issn","1365-2559"],["dc.relation.issn","0309-0167"],["dc.title","Metastatic type A thymoma: morphological and genetic correlation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","323"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Virchows Archiv"],["dc.bibliographiccitation.lastpage","327"],["dc.bibliographiccitation.volume","476"],["dc.contributor.author","Porubsky, Stefan"],["dc.contributor.author","Jessup, Peter"],["dc.contributor.author","Kee, Damien"],["dc.contributor.author","Sharma, Rajiv"],["dc.contributor.author","Ochi, Ayame"],["dc.contributor.author","Xu, Huiling"],["dc.contributor.author","Froelich, Jens J."],["dc.contributor.author","Nott, Louise"],["dc.contributor.author","Scott, Clare"],["dc.contributor.author","Awad, Raef"],["dc.contributor.author","Moldovan, Cristina"],["dc.contributor.author","Hardikar, Ashutosh A."],["dc.contributor.author","Bohnenberger, Hanibal"],["dc.contributor.author","Küffer, Stefan"],["dc.contributor.author","Ströbel, Philipp"],["dc.contributor.author","Marx, Alexander"],["dc.date.accessioned","2020-12-10T14:10:36Z"],["dc.date.available","2020-12-10T14:10:36Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1007/s00428-019-02644-3"],["dc.identifier.eissn","1432-2307"],["dc.identifier.issn","0945-6317"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/70816"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Potentially actionable FGFR2 high-level amplification in thymic sebaceous carcinoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","33"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","The Journal of Pathology: Clinical Research"],["dc.bibliographiccitation.lastpage","47"],["dc.bibliographiccitation.volume","8"],["dc.contributor.affiliation","Fichtner, Alexander; 1\r\nInstitute of Pathology\r\nUniversity Medical Centre Göttingen\r\nGöttingen Germany"],["dc.contributor.affiliation","Joost, Jasmin; 1\r\nInstitute of Pathology\r\nUniversity Medical Centre Göttingen\r\nGöttingen Germany"],["dc.contributor.affiliation","Brockmeyer, Philipp; 2\r\nDepartment of Oral and Maxillofacial Surgery\r\nUniversity Medical Centre Göttingen\r\nGöttingen Germany"],["dc.contributor.affiliation","Kauffmann, Philipp; 2\r\nDepartment of Oral and Maxillofacial Surgery\r\nUniversity Medical Centre Göttingen\r\nGöttingen Germany"],["dc.contributor.affiliation","Schliephake, Henning; 2\r\nDepartment of Oral and Maxillofacial Surgery\r\nUniversity Medical Centre Göttingen\r\nGöttingen Germany"],["dc.contributor.affiliation","Hammerstein‐Equord, Alexander; 3\r\nDepartment of Thoracic and Cardiovascular Surgery\r\nUniversity Medical Centre Göttingen\r\nGöttingen Germany"],["dc.contributor.affiliation","Kueffer, Stefan; 1\r\nInstitute of Pathology\r\nUniversity Medical Centre Göttingen\r\nGöttingen Germany"],["dc.contributor.affiliation","Urlaub, Henning; 4\r\nBioanalytical Mass Spectrometry Group\r\nMax Planck Institute for Biophysical Chemistry\r\nGöttingen Germany"],["dc.contributor.affiliation","Oellerich, Thomas; 6\r\nDepartment of Medicine II, Haematology/Oncology\r\nGoethe University\r\nFrankfurt Germany"],["dc.contributor.affiliation","Ströbel, Philipp; 1\r\nInstitute of Pathology\r\nUniversity Medical Centre Göttingen\r\nGöttingen Germany"],["dc.contributor.affiliation","Bohnenberger, Hanibal; 1\r\nInstitute of Pathology\r\nUniversity Medical Centre Göttingen\r\nGöttingen Germany"],["dc.contributor.affiliation","Bremmer, Felix; 1\r\nInstitute of Pathology\r\nUniversity Medical Centre Göttingen\r\nGöttingen Germany"],["dc.contributor.author","Richter, Annika"],["dc.contributor.author","Fichtner, Alexander"],["dc.contributor.author","Joost, Jasmin"],["dc.contributor.author","Brockmeyer, Philipp"],["dc.contributor.author","Kauffmann, Philipp"],["dc.contributor.author","Schliephake, Henning"],["dc.contributor.author","Hammerstein‐Equord, Alexander"],["dc.contributor.author","Kueffer, Stefan"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Oellerich, Thomas"],["dc.contributor.author","Bremmer, Felix"],["dc.contributor.author","Ströbel, Philipp"],["dc.contributor.author","Bohnenberger, Hanibal"],["dc.date.accessioned","2021-12-01T09:23:20Z"],["dc.date.available","2021-12-01T09:23:20Z"],["dc.date.issued","2021"],["dc.date.updated","2022-03-20T23:05:20Z"],["dc.description.abstract","Abstract The differentiation between a pulmonary metastasis and a newly developed squamous cell carcinoma of the lung in patients with prior head and neck squamous cell carcinoma (HNSCC) is difficult due to a lack of biomarkers but is crucially important for the prognosis and therapy of the affected patient. By using high‐resolution mass spectrometry in combination with stable isotope labelling by amino acids in cell culture, we identified 379 proteins that are differentially expressed in squamous cell carcinomas of the lung and the head and neck. Of those, CAV1, CAV2, LGALS1, LGALS7, CK19, and UGDH were tested by immunohistochemistry on 194 tissue samples (98 lung and 96 HNSCCs). The combination of CAV1 and LGALS7 was able to distinguish the origin of the squamous cell carcinoma with high accuracy (area under the curve 0.876). This biomarker panel was tested on a cohort of 12 clinically classified lung tumours of unknown origin after HNSCC. Nine of those tumours were immunohistochemically classifiable."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1002/cjp2.244"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94624"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.eissn","2056-4538"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","Quantitative proteomics identifies biomarkers to distinguish pulmonary from head and neck squamous cell carcinomas by immunohistochemistry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4620"],["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Cancer Research"],["dc.bibliographiccitation.lastpage","4632"],["dc.bibliographiccitation.volume","80"],["dc.contributor.author","Patil, Shilpa"],["dc.contributor.author","Steuber, Benjamin"],["dc.contributor.author","Kopp, Waltraut"],["dc.contributor.author","Kari, Vijayalakshmi"],["dc.contributor.author","Urbach, Laura"],["dc.contributor.author","Wang, Xin"],["dc.contributor.author","Küffer, Stefan"],["dc.contributor.author","Bohnenberger, Hanibal"],["dc.contributor.author","Spyropoulou, Dimitra"],["dc.contributor.author","Zhang, Zhe"],["dc.contributor.author","Versemann, Lennart"],["dc.contributor.author","Bösherz, Mark Sebastian"],["dc.contributor.author","Brunner, Marius"],["dc.contributor.author","Gaedcke, Jochen"],["dc.contributor.author","Ströbel, Philipp"],["dc.contributor.author","Zhang, Jin-San"],["dc.contributor.author","Neesse, Albrecht"],["dc.contributor.author","Ellenrieder, Volker"],["dc.contributor.author","Singh, Shiv K."],["dc.contributor.author","Johnsen, Steven A."],["dc.contributor.author","Hessmann, Elisabeth"],["dc.date.accessioned","2021-04-14T08:31:24Z"],["dc.date.available","2021-04-14T08:31:24Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1158/0008-5472.CAN-20-0672"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83584"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1538-7445"],["dc.relation.issn","0008-5472"],["dc.title","EZH2 Regulates Pancreatic Cancer Subtype Identity and Tumor Progression via Transcriptional Repression of GATA6"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Metabolites"],["dc.bibliographiccitation.volume","12"],["dc.contributor.affiliation","Li, Yuchan; 1Institute of Pathology, University Medical Center, 37075 Göttingen, Germany; yuchan.li@stud.uni-goettingen.de (Y.L.); omarakkad14@gmail.com (O.E.); yaosha2016@gmail.com (S.Y.); carmelo.ferrai@med.uni-goettingen.de (C.F.); philipp.stroebel@med.uni-goettingen.de (P.S.)"],["dc.contributor.affiliation","Elakad, Omar; 1Institute of Pathology, University Medical Center, 37075 Göttingen, Germany; yuchan.li@stud.uni-goettingen.de (Y.L.); omarakkad14@gmail.com (O.E.); yaosha2016@gmail.com (S.Y.); carmelo.ferrai@med.uni-goettingen.de (C.F.); philipp.stroebel@med.uni-goettingen.de (P.S.)"],["dc.contributor.affiliation","Yao, Sha; 1Institute of Pathology, University Medical Center, 37075 Göttingen, Germany; yuchan.li@stud.uni-goettingen.de (Y.L.); omarakkad14@gmail.com (O.E.); yaosha2016@gmail.com (S.Y.); carmelo.ferrai@med.uni-goettingen.de (C.F.); philipp.stroebel@med.uni-goettingen.de (P.S.)"],["dc.contributor.affiliation","von Hammerstein-Equord, Alexander; 4Department of Thoracic and Cardiovascular Surgery, University Medical Center, 37075 Göttingen, Germany; alexander.hammerstein@med.uni-goettingen.de (A.v.H.-E.); marc.hinterthaner@med.uni-goettingen.de (M.H.); bernd.danner@med.uni-goettingen.de (B.C.D.)"],["dc.contributor.affiliation","Hinterthaner, Marc; 4Department of Thoracic and Cardiovascular Surgery, University Medical Center, 37075 Göttingen, Germany; alexander.hammerstein@med.uni-goettingen.de (A.v.H.-E.); marc.hinterthaner@med.uni-goettingen.de (M.H.); bernd.danner@med.uni-goettingen.de (B.C.D.)"],["dc.contributor.affiliation","Danner, Bernhard C.; 4Department of Thoracic and Cardiovascular Surgery, University Medical Center, 37075 Göttingen, Germany; alexander.hammerstein@med.uni-goettingen.de (A.v.H.-E.); marc.hinterthaner@med.uni-goettingen.de (M.H.); bernd.danner@med.uni-goettingen.de (B.C.D.)"],["dc.contributor.affiliation","Ferrai, Carmelo; 1Institute of Pathology, University Medical Center, 37075 Göttingen, Germany; yuchan.li@stud.uni-goettingen.de (Y.L.); omarakkad14@gmail.com (O.E.); yaosha2016@gmail.com (S.Y.); carmelo.ferrai@med.uni-goettingen.de (C.F.); philipp.stroebel@med.uni-goettingen.de (P.S.)"],["dc.contributor.affiliation","Ströbel, Philipp; 1Institute of Pathology, University Medical Center, 37075 Göttingen, Germany; yuchan.li@stud.uni-goettingen.de (Y.L.); omarakkad14@gmail.com (O.E.); yaosha2016@gmail.com (S.Y.); carmelo.ferrai@med.uni-goettingen.de (C.F.); philipp.stroebel@med.uni-goettingen.de (P.S.)"],["dc.contributor.affiliation","Küffer, Stefan; 1Institute of Pathology, University Medical Center, 37075 Göttingen, Germany; yuchan.li@stud.uni-goettingen.de (Y.L.); omarakkad14@gmail.com (O.E.); yaosha2016@gmail.com (S.Y.); carmelo.ferrai@med.uni-goettingen.de (C.F.); philipp.stroebel@med.uni-goettingen.de (P.S.)"],["dc.contributor.affiliation","Bohnenberger, Hanibal; 1Institute of Pathology, University Medical Center, 37075 Göttingen, Germany; yuchan.li@stud.uni-goettingen.de (Y.L.); omarakkad14@gmail.com (O.E.); yaosha2016@gmail.com (S.Y.); carmelo.ferrai@med.uni-goettingen.de (C.F.); philipp.stroebel@med.uni-goettingen.de (P.S.)"],["dc.contributor.author","Li, Yuchan"],["dc.contributor.author","Elakad, Omar"],["dc.contributor.author","Yao, Sha"],["dc.contributor.author","von Hammerstein-Equord, Alexander"],["dc.contributor.author","Hinterthaner, Marc"],["dc.contributor.author","Danner, Bernhard C."],["dc.contributor.author","Ferrai, Carmelo"],["dc.contributor.author","Ströbel, Philipp"],["dc.contributor.author","Küffer, Stefan"],["dc.contributor.author","Bohnenberger, Hanibal"],["dc.date.accessioned","2022-08-04T08:28:51Z"],["dc.date.available","2022-08-04T08:28:51Z"],["dc.date.issued","2022-07-15"],["dc.date.updated","2022-08-03T15:44:46Z"],["dc.description.abstract","Activating KRAS mutations occur in about 30% of pulmonary adenocarcinoma (AC) cases and the discovery of specific inhibitors of G12C-mutated KRAS has considerably improved the prognosis for a subgroup of about 14% of non-small cell lung cancer (NSCLC) patients. However, even in patients with a KRAS G12C mutation, the overall response rate only reaches about 40% and mutations other than G12C still cannot be targeted. Despite the fact that one-carbon metabolism (1CM) and epigenetic regulation are known to be dysregulated by aberrant KRAS activity, we still lack evidence that co-treatment with drugs that regulate these factors might ameliorate response rates and patient prognosis. In this study, we show a direct dependency of Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) and Enhancer of Zeste Homolog 2 (EZH2) expression on mutationally activated KRAS and their prognostic relevance in KRAS-mutated AC. We show that aberrant KRAS activity generates a vulnerability of AC cancer cell lines to both MTHFD2 and EZH2 inhibitors. Importantly, co-inhibition of both factors was synergistically effective and comparable to KRASG12C inhibition alone, paving the way for their use in a therapeutic approach for NSCLC cancer patients."],["dc.description.sponsorship","Deutsche Krebshilfe Foundation"],["dc.description.sponsorship","University Medical Center Göttingen"],["dc.description.sponsorship","Else-Kröner-Fresenius-Foundation"],["dc.description.sponsorship","Chinese Scholarship Counsil"],["dc.description.sponsorship","DFG"],["dc.identifier.doi","10.3390/metabo12070652"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112626"],["dc.language.iso","en"],["dc.relation.eissn","2218-1989"],["dc.rights","CC BY 4.0"],["dc.title","Regulation and Therapeutic Targeting of MTHFD2 and EZH2 in KRAS-Mutated Human Pulmonary Adenocarcinoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Journal of Thoracic Oncology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Bohnenberger, Hanibal"],["dc.contributor.author","Yepes, Diego"],["dc.contributor.author","Pan, Kuan-Ting"],["dc.contributor.author","Emmert, Alexander"],["dc.contributor.author","Henric-Petri, Hannah"],["dc.contributor.author","Bremmer, Felix"],["dc.contributor.author","Strecker, Jasmin"],["dc.contributor.author","Lois, Anna-Maria"],["dc.contributor.author","Fischer, Lucas"],["dc.contributor.author","Kueffer, Stefan"],["dc.contributor.author","Hinterthaner, Marc"],["dc.contributor.author","Wolff, Hendrik"],["dc.contributor.author","Canis, Martin"],["dc.contributor.author","Sebastian, Martin"],["dc.contributor.author","Danner, Bernd"],["dc.contributor.author","Stroebel, Philipp"],["dc.contributor.author","Serve, Hubert"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Oellerich, Thomas"],["dc.date.accessioned","2018-11-07T09:51:51Z"],["dc.date.available","2018-11-07T09:51:51Z"],["dc.date.issued","2015"],["dc.format.extent","S293"],["dc.identifier.isi","000370365101087"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35995"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.publisher.place","New york"],["dc.relation.issn","1556-1380"],["dc.relation.issn","1556-0864"],["dc.title","Proteomic Profiling of Pulmonary Cancer with Squamous Cell Histology"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Disease Markers"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","2019"],["dc.contributor.author","Bremmer, Felix"],["dc.contributor.author","Bohnenberger, Hanibal"],["dc.contributor.author","Küffer, Stefan"],["dc.contributor.author","Oellerich, Thomas"],["dc.contributor.author","Serve, Hubert"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Strauß, Arne"],["dc.contributor.author","Maatoug, Yasmine"],["dc.contributor.author","Behnes, Carl Ludwig"],["dc.contributor.author","Oing, Christoph"],["dc.contributor.author","Radzun, Heinz Joachim"],["dc.contributor.author","Ströbel, Philipp"],["dc.contributor.author","Balabanov, Stefan"],["dc.contributor.author","Honecker, Friedemann"],["dc.date.accessioned","2019-09-24T07:40:13Z"],["dc.date.available","2019-09-24T07:40:13Z"],["dc.date.issued","2019"],["dc.description.abstract","Malignant germ cell tumors (GCT) are the most common malignant tumors in young men between 18 and 40 years. The correct identification of histological subtypes, in difficult cases supported by immunohistochemistry, is essential for therapeutic management. Furthermore, biomarkers may help to understand pathophysiological processes in these tumor types. Two GCT cell lines, TCam-2 with seminoma-like characteristics, and NTERA-2, an embryonal carcinoma-like cell line, were compared by a quantitative proteomic approach using high-resolution mass spectrometry (MS) in combination with stable isotope labelling by amino acid in cell culture (SILAC). We were able to identify 4856 proteins and quantify the expression of 3936. 347 were significantly differentially expressed between the two cell lines. For further validation, CD81, CBX-3, PHF6, and ENSA were analyzed by western blot analysis. The results confirmed the MS results. Immunohistochemical analysis on 59 formalin-fixed and paraffin-embedded (FFPE) normal and GCT tissue samples (normal testis, GCNIS, seminomas, and embryonal carcinomas) of these proteins demonstrated the ability to distinguish different GCT subtypes, especially seminomas and embryonal carcinomas. In addition, siRNA-mediated knockdown of these proteins resulted in an antiproliferative effect in TCam-2, NTERA-2, and an additional embryonal carcinoma-like cell line, NCCIT. In summary, this study represents a proteomic resource for the discrimination of malignant germ cell tumor subtypes and the observed antiproliferative effect after knockdown of selected proteins paves the way for the identification of new potential drug targets."],["dc.identifier.doi","10.1155/2019/8298524"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16378"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62439"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0278-0240"],["dc.relation.issn","1875-8630"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e154475"],["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","JCI Insight"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Krebs, Niklas"],["dc.contributor.author","Klein, Lukas"],["dc.contributor.author","Wegwitz, Florian"],["dc.contributor.author","Espinet, Elisa"],["dc.contributor.author","Maurer, Hans Carlo"],["dc.contributor.author","Tu, Mengyu"],["dc.contributor.author","Penz, Frederike"],["dc.contributor.author","Küffer, Stefan"],["dc.contributor.author","Xu, Xingbo"],["dc.contributor.author","Bohnenberger, Hanibal"],["dc.contributor.author","Singh, Shiv K."],["dc.date.accessioned","2022-09-01T09:50:53Z"],["dc.date.available","2022-09-01T09:50:53Z"],["dc.date.issued","2022"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.1172/jci.insight.154475"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113823"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.relation.eissn","2379-3708"],["dc.title","Axon guidance receptor ROBO3 modulates subtype identity and prognosis via AXL-associated inflammatory network in pancreatic cancer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]