Kerschensteiner, Martin

[["dc.bibliographiccitation.firstpage","2461"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Annals of Clinical and Translational Neurology"],["dc.bibliographiccitation.lastpage","2466"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Penkert, Horst"],["dc.contributor.author","Lauber, Chris"],["dc.contributor.author","Gerl, Mathias J."],["dc.contributor.author","Klose, Christian"],["dc.contributor.author","Damm, Markus"],["dc.contributor.author","Fitzner, Dirk"],["dc.contributor.author","Flierl‐Hecht, Andrea"],["dc.contributor.author","Kümpfel, Tania"],["dc.contributor.author","Kerschensteiner, Martin"],["dc.contributor.author","Hohlfeld, Reinhard"],["dc.contributor.author","Gerdes, Lisa A."],["dc.contributor.author","Simons, Mikael"],["dc.date.accessioned","2021-04-14T08:32:24Z"],["dc.date.available","2021-04-14T08:32:24Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1002/acn3.51216"],["dc.identifier.pmid","33159711"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83910"],["dc.identifier.url","https://rdp.sfb274.de/literature/publications/1"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","TRR 274: Checkpoints of Central Nervous System Recovery"],["dc.relation","TRR 274 | B01: The role of inflammatory cytokine signaling for efficient remyelination in multiple sclerosis"],["dc.relation","TRR 274 | C02: In vivo detection and targeting of calcium clearance and axonal membrane repair after acute CNS insults"],["dc.relation.issn","2328-9503"],["dc.relation.workinggroup","RG Kerschensteiner (Neuroimmune Interactions)"],["dc.relation.workinggroup","RG Simons (The Biology of Glia in Development and Disease)"],["dc.title","Plasma lipidomics of monozygotic twins discordant for multiple sclerosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","100232"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","STAR Protocols"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Kislinger, Georg"],["dc.contributor.author","Gnägi, Helmut"],["dc.contributor.author","Kerschensteiner, Martin"],["dc.contributor.author","Simons, Mikael"],["dc.contributor.author","Misgeld, Thomas"],["dc.contributor.author","Schifferer, Martina"],["dc.date.accessioned","2022-08-19T06:32:51Z"],["dc.date.available","2022-08-19T06:32:51Z"],["dc.date.issued","2020"],["dc.description.abstract","Here, we describe a detailed workflow for ATUM-FIB microscopy, a hybrid method that combines serial-sectioning scanning electron microscopy (SEM) with focused ion beam SEM (FIB-SEM). This detailed protocol is optimized for mouse cortex samples. The main processing steps include the generation of semi-thick sections from sequentially cured resin blocks using a heated microtomy approach. We demonstrate the different imaging modalities, including serial light and electron microscopy for target recognition and FIB-SEM for isotropic imaging of regions of interest. For complete details on the use and execution of this protocol, please refer to Kislinger et al. (2020)."],["dc.identifier.doi","10.1016/j.xpro.2020.100232"],["dc.identifier.pmid","33377119"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113013"],["dc.identifier.url","https://rdp.sfb274.de/literature/publications/33"],["dc.language.iso","en"],["dc.relation","TRR 274: Checkpoints of Central Nervous System Recovery"],["dc.relation","TRR 274 | B03: Checkpoints of recovery after primary astrocytic lesions in neuromyelitis optica and related animal models"],["dc.relation","TRR 274 | C02: In vivo detection and targeting of calcium clearance and axonal membrane repair after acute CNS insults"],["dc.relation","TRR 274 | Z01: Bioimaging Platform"],["dc.relation.eissn","2666-1667"],["dc.relation.workinggroup","RG Kerschensteiner (Neuroimmune Interactions)"],["dc.relation.workinggroup","RG Misgeld"],["dc.relation.workinggroup","RG Schifferer"],["dc.relation.workinggroup","RG Simons (The Biology of Glia in Development and Disease)"],["dc.title","ATUM-FIB microscopy for targeting and multiscale imaging of rare events in mouse cortex"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","101290"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","iScience"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Kislinger, Georg"],["dc.contributor.author","Gnägi, Helmut"],["dc.contributor.author","Kerschensteiner, Martin"],["dc.contributor.author","Simons, Mikael"],["dc.contributor.author","Misgeld, Thomas"],["dc.contributor.author","Schifferer, Martina"],["dc.date.accessioned","2022-08-18T13:58:23Z"],["dc.date.available","2022-08-18T13:58:23Z"],["dc.date.issued","2020-07-24"],["dc.description.abstract","Volume electron microscopy enables the ultrastructural analysis of biological tissue. Currently, the techniques involving ultramicrotomy (ATUM, ssTEM) allow large fields of view but afford only limited z-resolution, whereas ion beam-milling approaches (FIB-SEM) yield isotropic voxels but are restricted in volume size. Now we present a hybrid method, named ATUM-FIB, which combines the advantages of both approaches. ATUM-FIB is based on serial sectioning of tissue into \"semithick\" (2-10 μm) sections collected onto tape. Serial light and electron microscopy allows the identification of regions of interest that are then directly accessible for targeted FIB-SEM. The set of semithick sections thus represents a tissue \"library\" which provides three-dimensional context information that can be probed \"on demand\" by local high-resolution analysis. We demonstrate the potential of this technique to reveal the ultrastructure of rare but pathologically important events by identifying microglia contact sites with amyloid plaques in a mouse model of familial Alzheimer's disease."],["dc.identifier.doi","10.1016/j.isci.2020.101290"],["dc.identifier.pmid","32622266"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113006"],["dc.identifier.url","https://rdp.sfb274.de/literature/publications/15"],["dc.language.iso","en"],["dc.relation","TRR 274: Checkpoints of Central Nervous System Recovery"],["dc.relation","TRR 274 | B03: Checkpoints of recovery after primary astrocytic lesions in neuromyelitis optica and related animal models"],["dc.relation","TRR 274 | C02: In vivo detection and targeting of calcium clearance and axonal membrane repair after acute CNS insults"],["dc.relation","TRR 274 | Z01: Bioimaging Platform"],["dc.relation.eissn","2589-0042"],["dc.relation.workinggroup","RG Kerschensteiner (Neuroimmune Interactions)"],["dc.relation.workinggroup","RG Misgeld"],["dc.relation.workinggroup","RG Schifferer"],["dc.relation.workinggroup","RG Simons (The Biology of Glia in Development and Disease)"],["dc.title","Multiscale ATUM-FIB Microscopy Enables Targeted Ultrastructural Analysis at Isotropic Resolution"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]