Weber, Klaus W.

[["dc.bibliographiccitation.firstpage","101"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","RNA Biology"],["dc.bibliographiccitation.lastpage","105"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Gruber, Jens"],["dc.contributor.author","Manninga, Heiko"],["dc.contributor.author","Tuschl, Thomas"],["dc.contributor.author","Osborn, Mary"],["dc.contributor.author","Weber, Klaus"],["dc.date.accessioned","2018-11-22T09:28:16Z"],["dc.date.available","2018-11-22T09:28:16Z"],["dc.date.issued","2014"],["dc.identifier.doi","10.4161/rna.2.3.2060"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56919"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.title","Specific RNAi Mediated Gene Knockdown in Zebrafish Cell Lines"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","75"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Experimental Cell Research"],["dc.bibliographiccitation.lastpage","86"],["dc.bibliographiccitation.volume","286"],["dc.contributor.author","Bechert, Kim"],["dc.contributor.author","Lagos-Quintana, Mariana"],["dc.contributor.author","Harborth, Jens"],["dc.contributor.author","Weber, Klaus"],["dc.contributor.author","Osborn, Mary"],["dc.date.accessioned","2018-11-22T09:34:04Z"],["dc.date.available","2018-11-22T09:34:04Z"],["dc.date.issued","2003"],["dc.identifier.doi","10.1016/S0014-4827(03)00104-6"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56920"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Effects of expressing lamin A mutant protein causing Emery-Dreifuss muscular dystrophy and familial partial lipodystrophy in Hela cells"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1689"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","The EMBO Journal"],["dc.bibliographiccitation.lastpage","1700"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Harborth, J."],["dc.contributor.author","Wang, J."],["dc.contributor.author","Gueth-Hallonet, C."],["dc.contributor.author","Weber, K."],["dc.contributor.author","Osborn, M."],["dc.date.accessioned","2018-11-21T14:22:03Z"],["dc.date.available","2018-11-21T14:22:03Z"],["dc.date.issued","1999"],["dc.description.abstract","NuMA is a nuclear matrix protein in interphase and relocates to the spindle poles in mitotis. Different NuMA constructs, in which either N- or C-terminal domains were deleted, and the full-length construct were expressed in Escherichia coli, and the NuMA polypeptides were purified to homogeneity and allowed to assemble in vitro. Electron microscopy showed that NuMA can build multiarm oligomers by interaction of the C-terminal globular domains. Each arm of the oligomer corresponds to a NuMA dimer. Oligomers with up to 10 or 12 arms have been observed for both full-length NuMA and for constructs that still contain the proximal part of the C-terminal tail domain. Other results from this laboratory have shown that transient overexpression of NuMA in HeLa cells induces a nuclear scaffold with a quasi-hexagonal organization that can fill the nuclei. Here we show that computer modelling of the three-dimensional packing of NuMA into such scaffolds can explain the different spacing of the hexagons seen when constructs with different coiled-coil lengths are used. Thus, the 12 arm oligomer, for which we have in vitro evidence, may be the structural unit from which the nuclear scaffold in transfected cells is built."],["dc.identifier.doi","10.1093/emboj/18.6.1689"],["dc.identifier.pmid","10075938"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56908"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Self assembly of NuMA: multiarm oligomers as structural units of a nuclear lattice"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","75"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","European Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","87"],["dc.bibliographiccitation.volume","57"],["dc.contributor.author","Meyer, T."],["dc.contributor.author","Weber, K."],["dc.contributor.author","Osborn, M."],["dc.date.accessioned","2018-11-21T14:25:43Z"],["dc.date.available","2018-11-21T14:25:43Z"],["dc.date.issued","1992"],["dc.description.abstract","The murine monoclonal IFA antibody recognizes a conserved sequence present in almost all intermediate filament (IF) proteins. When IFA antibody was injected into 13 different primary or established cell lines, striking differences were detected between epithelial and fibroblastic cell lines. In epithelial cells keratin IFs were broken down within 4 h into numerous spheroid aggregates scattered throughout the cytoplasm. Keratin aggregates were first detected in the cytoplasmic periphery. In contrast, in fibroblastic cells, injection of IFA antibody led to the formation of perinuclear coils of vimentin. IFA antibody at a concentration of greater than 1 mg/ml had to be injected to initiate these transitions. When HeLa cells, which contain separate networks of vimentin and keratin filaments, were injected with IFA antibody, vimentin did not form perinuclear coils but was instead found together with keratin in aggregates. Electron micrographs of HeLa cells injected with IFA antibody showed that the aggregates have diameters between 0.5 and 2.6 microns and resembled the keratin aggregates observed in certain mitotic epithelial cells. Although the ultrastructural studies support an association of some aggregates with desmosomes, aggregates were, however, also induced by injection of IFA antibody into human keratinocytes in low calcium medium under conditions where desmosomes were not present."],["dc.identifier.pmid","1379181"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56909"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Microinjection of IFA antibody induces intermediate filament aggregates in epithelial cell lines but perinuclear coils in fibroblast-like lines"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","82"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","RNA Biology"],["dc.bibliographiccitation.lastpage","89"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Hossbach, Markus"],["dc.contributor.author","Gruber, Jens"],["dc.contributor.author","Osborn, Mary"],["dc.contributor.author","Weber, Klaus"],["dc.contributor.author","Tuschl, Thomas"],["dc.date.accessioned","2018-11-21T14:09:38Z"],["dc.date.available","2018-11-21T14:09:38Z"],["dc.date.issued","2006"],["dc.description.abstract","Synthetic small interfering RNA (siRNA) duplexes are widely used to transiently and sequence-specifically disrupt gene expression in mammalian cultured cells. The efficiency and specificity of mRNA cleavage is partly affected by the presence of the nontargeting \"passenger\" or \"sense\" siRNA strand, which is required for presentation of the target-complementary or guide siRNA strand to the double-strand-specific RNA silencing protein machinery. We show that siRNA duplexes can be designed that are solely composed of two fully target-complementary guide strands that are sufficiently complementary to each other to form stable duplexes with characteristic 3' overhanging ends. The general feasibility of this approach is documented by transient knockdown of lamin A/C and emerin in HeLa cells. The silencing efficiencies of guide-only siRNA duplexes are comparable to prototypical fully paired passenger/guide duplex siRNAs, even though guide-only siRNA duplexes may contain a significant number of nonWatson-Crick and G/U wobble base pairs. Such siRNA duplexes may offer advantages regarding production costs and specificity of gene silencing."],["dc.identifier.doi","10.4161/rna.3.2.3110"],["dc.identifier.pmid","17114944"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56907"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1555-8584"],["dc.title","Gene silencing with siRNA duplexes composed of target-mRNA-complementary and partially palindromic or partially complementary single-stranded siRNAs"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","185"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Experimental Cell Research"],["dc.bibliographiccitation.lastpage","194"],["dc.bibliographiccitation.volume","190"],["dc.contributor.author","Röber, Ruth-Ariane"],["dc.contributor.author","Gieseler, Robert K. H."],["dc.contributor.author","Peters, J. Hinrich"],["dc.contributor.author","Weber, Klaus"],["dc.contributor.author","Osborn, Mary"],["dc.date.accessioned","2018-11-22T09:37:25Z"],["dc.date.available","2018-11-22T09:37:25Z"],["dc.date.issued","1990"],["dc.identifier.doi","10.1016/0014-4827(90)90184-C"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56921"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Induction of nuclear lamins A/C in macrophages in in vitro cultures of rat bone marrow precursor cells and human blood monocytes, and in macrophages elicited in vivo by thioglycollate stimulation"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]