Bartels, Matthias

[["dc.bibliographiccitation.artnumber","10"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Optical Nanoscopy"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Krüger, Sven P"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Olendrowitz, Christian"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:54:07Z"],["dc.date.available","2017-09-07T11:54:07Z"],["dc.date.issued","2012"],["dc.description.abstract","We have imaged the three-dimensional density distribution of unstained and unsliced, freeze-dried cells of the gram-positive bacterium Deinococcus radiodurans by tomographic x-ray propagation microscopy, i.e. projection tomography with phase contrast formation by free space propagation. The work extends previous x-ray imaging of biological cells in the simple in-line holography geometry to full three-dimensional reconstruction, based on a fast iterative phase reconstruction algorithm which circumvents the usual twin-image problem. The sample is illuminated by the highly curved wave fronts emitted from a virtual quasi-point source with 10 nm cross section, realized by two crossed x-ray waveguides. The experimental scheme allows for a particularly dose efficient determination of the 3D density distribution in the cellular structure."],["dc.identifier.doi","10.1186/2192-2853-1-10"],["dc.identifier.fs","593648"],["dc.identifier.gro","3145116"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9581"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2817"],["dc.language.iso","en"],["dc.notes","Funding by the DFG collaborative research center SFB 755\r\nNanoscale Photonic Imaging and the German Ministry of Education and\r\nResearch (Grant No. 05K10MGA) is gratefully acknowledged."],["dc.notes.intern","Crossref Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.issn","2192-2853"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Low-dose three-dimensional hard x-ray imaging of bacterial cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","09973"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Markus, Andrea"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:44:24Z"],["dc.date.available","2017-09-07T11:44:24Z"],["dc.date.issued","2015"],["dc.description.abstract","We have performed x-ray phase-contrast tomography on mouse lung tissue. Using a divergent x-ray beam generated by nanoscale focusing, we used zoom tomography to produce three-dimensional reconstructions with selectable magnification, resolution, and field of view. Thus, macroscopic tissue samples extending over several mm can be studied in sub-cellular-level structural detail. The zoom capability and, in particular, the high dose efficiency are enabled by the near-perfect exit wavefront of an optimized x-ray waveguide channel. In combination with suitable phase-retrieval algorithms, challenging radiation-sensitive and low-contrast samples can be reconstructed with minimal artefacts. The dose efficiency of the method is demonstrated by the reconstruction of living macrophages both with and without phagocytized contrast agents. We also used zoom tomography to visualize barium-labelled macrophages in the context of morphological structures in asthmatic and healthy mouse lung tissue one day after intratracheal application. The three-dimensional reconstructions showed that the macrophages predominantly localized to the alveoli, but they were also found in bronchial walls, indicating that these cells might be able to migrate from the lumen of the bronchi through the epithelium."],["dc.identifier.doi","10.1038/srep09973"],["dc.identifier.fs","615791"],["dc.identifier.gro","3141904"],["dc.identifier.isi","000354310200001"],["dc.identifier.pmid","25966338"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13625"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2367"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2045-2322"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Cell Line, Transformed"],["dc.subject.mesh","Cell Movement"],["dc.subject.mesh","Macrophages, Alveolar"],["dc.subject.mesh","Mice"],["dc.subject.mesh","Pulmonary Alveoli"],["dc.subject.mesh","Respiratory Mucosa"],["dc.subject.mesh","Tomography, X-Ray"],["dc.title","Phase-contrast zoom tomography reveals precise locations of macrophages in mouse lungs"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]