Krawczak, Michael

[["dc.bibliographiccitation.firstpage","1109"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Biology of Reproduction"],["dc.bibliographiccitation.lastpage","1113"],["dc.bibliographiccitation.volume","72"],["dc.contributor.author","Krawczak, Michael"],["dc.contributor.author","Trefilov, Andrea"],["dc.contributor.author","Berard, John"],["dc.contributor.author","Bercovitch, Fred"],["dc.contributor.author","Kessler, Matthew"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Croucher, Peter"],["dc.contributor.author","Nürnberg, Peter"],["dc.contributor.author","Widdig, Anja"],["dc.contributor.author","Schmidtke, Jörg"],["dc.date.accessioned","2022-10-06T13:35:19Z"],["dc.date.available","2022-10-06T13:35:19Z"],["dc.date.issued","2005"],["dc.identifier.doi","10.1095/biolreprod.104.038059"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116066"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1529-7268"],["dc.relation.issn","0006-3363"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Male Reproductive Timing in Rhesus Macaques Is Influenced by the 5HTTLPR Promoter Polymorphism of the Serotonin Transporter Gene1"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0224082"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Winkler, Michael"],["dc.contributor.author","Gärtner, Sabine"],["dc.contributor.author","Markus, Lara"],["dc.contributor.author","Hoffmann, Markus"],["dc.contributor.author","Nehlmeier, Inga"],["dc.contributor.author","Krawczak, Michael"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.date.accessioned","2019-11-05T08:51:28Z"],["dc.date.accessioned","2021-10-27T13:13:08Z"],["dc.date.available","2019-11-05T08:51:28Z"],["dc.date.available","2021-10-27T13:13:08Z"],["dc.date.issued","2019"],["dc.description.abstract","The experimental infection of rhesus macaques (rh) with simian immunodeficiency virus (SIV) is an important model for human immunodeficiency virus (HIV) infection of humans. The interferon-induced transmembrane protein 3 (IFITM3) inhibits HIV and SIV infection at the stage of host cell entry. However, it is still unclear to what extent the antiviral activity of IFITM3 observed in cell culture translates into inhibition of HIV/SIV spread in the infected host. We have shown previously that although rhIFITM3 inhibits SIV entry into cultured cells, polymorphisms in the rhIFITM3 gene are not strongly associated with viral load or disease progression in SIV infected macaques. Here, we examined whether rhIFITM3(2), which is closely related to rhIFITM3 at the sequence level, exerts antiviral activity and whether polymorphisms in the rhIFITM3(2) gene impact the course of SIV infection. We show that expression of rhIFITM3(2) is interferon-inducible and inhibits SIV entry into cells, although with reduced efficiency as compared to rhIFITM3. We further report the identification of 19 polymorphisms in the rhIFITM3(2) gene. However, analysis of a well characterized cohort of SIV infected macaques revealed that none of the polymorphisms had a significant impact upon the course of SIV infection. These results and our previous work suggest that polymorphisms in the rhIFITM3 and rhIFITM3(2) genes do not strongly modulate the course of SIV infection in macaques."],["dc.identifier.doi","10.1371/journal.pone.0224082"],["dc.identifier.pmid","31682595"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16596"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91754"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.eissn","1932-6203"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","599"],["dc.title","Role of rhesus macaque IFITM3(2) in simian immunodeficiency virus infection of macaques"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e25474"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Siddiqui, Roman A."],["dc.contributor.author","Krawczak, Michael"],["dc.contributor.author","Platzer, Matthias"],["dc.contributor.author","Sauermann, Ulrike"],["dc.date.accessioned","2019-07-09T11:54:02Z"],["dc.date.available","2019-07-09T11:54:02Z"],["dc.date.issued","2011-10-13"],["dc.description.abstract","In HIV infection, TLR7-triggered IFN-a production exerts a direct antiviral effect through the inhibition of viral replication, but may also be involved in immune pathogenesis leading to AIDS. TLR7 could also be an important mediator of vaccine efficacy. In this study, we analyzed polymorphisms in the X-linked TLR7 gene in the rhesus macaque model of AIDS. Upon resequencing of the TLR7 gene in 36 rhesus macaques of Indian origin, 12 polymorphic sites were detected. Next, we identified three tightly linked single nucleotide polymorphisms (SNP) as being associated with survival time. Genotyping of 119 untreated, simian immunodeficiency virus (SIV)-infected male rhesus macaques, including an ‘MHC adjusted’ subset, revealed that the three TLR7 SNPs are also significantly associated with set-point viral load. Surprisingly, this effect was not observed in 72 immunized SIV-infected male monkeys. We hypothesize (i) that SNP c.13G.A in the leader peptide is causative for the observed genotype-phenotype association and that (ii) the underlying mechanism is related to RNA secondary structure formation. Therefore, we investigated a fourth SNP (c.-17C.T), located 17 bp upstream of the ATG translation initiation codon, that is also potentially capable of influencing RNA structure. In c.13A carriers, neither set-point viral load nor survival time were related to the c.-17C.T genotype. In c.13G carriers, by contrast, the c.-17C allele was significantly associated with prolonged survival. Again, no such association was detected among immunized SIV-infected macaques. Our results highlight the dual role of TLR7 in immunodeficiency virus infection and vaccination and imply that it may be important to control human AIDS vaccine trials, not only for MHC genotype, but also for TLR7 genotype."],["dc.format.extent","10"],["dc.identifier.doi","10.1371/journal.pone.0025474"],["dc.identifier.pmid","22022401"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8344"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60554"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Association of TLR7 Variants with AIDS-Like Disease and AIDS Vaccine Efficacy in Rhesus Macaques"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","Journal of Virology"],["dc.bibliographiccitation.volume","91"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Radaelli, Antonia"],["dc.contributor.author","Stolte-Leeb, Nicole"],["dc.contributor.author","Raue, Katharina"],["dc.contributor.author","Bissa, Massimiliano"],["dc.contributor.author","Zanotto, Carlo"],["dc.contributor.author","Krawczak, Michael"],["dc.contributor.author","Tenbusch, Matthias"],["dc.contributor.author","Überla, Klaus"],["dc.contributor.editor","Kirchhoff, Frank"],["dc.date.accessioned","2021-06-01T10:47:34Z"],["dc.date.available","2021-06-01T10:47:34Z"],["dc.date.issued","2017"],["dc.description.abstract","ABSTRACT An effective AIDS vaccine should elicit strong humoral and cellular immune responses while maintaining low levels of CD4 + T-cell activation to avoid the generation of target cells for viral infection. The present study investigated two prime-boost regimens, both starting vaccination with single-cycle immunodeficiency virus, followed by two mucosal boosts with either recombinant adenovirus (rAd) or fowlpox virus (rFWPV) expressing SIVmac239 or SIVmac251 gag/pol and env genes, respectively. Finally, vectors were switched and systemically administered to the reciprocal group of animals. Only mucosal rFWPV immunizations followed by systemic rAd boost significantly protected animals against a repeated low-dose intrarectal challenge with pathogenic SIVmac251, resulting in a vaccine efficacy (i.e., risk reduction per exposure) of 68%. Delayed viral acquisition was associated with higher levels of activated CD8 + T cells and Gag-specific gamma interferon (IFN-γ)-secreting CD8 + cells, low virus-specific CD4 + T-cell responses, and low Env antibody titers. In contrast, the systemic rFWPV boost induced strong virus-specific CD4 + T-cell activity. rAd and rFWPV also induced differential patterns of the innate immune responses, thereby possibly shaping the specific immunity. Plasma CXCL10 levels after final immunization correlated directly with virus-specific CD4 + T-cell responses and inversely with the number of exposures to infection. Also, the percentage of activated CD69 + CD8 + T cells correlated with the number of exposures to infection. Differential stimulation of the immune response likely provided the basis for the diverging levels of protection afforded by the vaccine regimen. IMPORTANCE A failed phase II AIDS vaccine trial led to the hypothesis that CD4 + T-cell activation can abrogate any potentially protective effects delivered by vaccination or promote acquisition of the virus because CD4 + T helper cells, required for an effective immune response, also represent the target cells for viral infection. We compared two vaccination protocols that elicited similar levels of Gag-specific immune responses in rhesus macaques. Only the animal group that had a low level of virus-specific CD4 + T cells in combination with high levels of activated CD8 + T cells was significantly protected from infection. Notably, protection was achieved despite the lack of appreciable Env antibody titers. Moreover, we show that both the vector and the route of immunization affected the level of CD4 + T-cell responses. Thus, mucosal immunization with FWPV-based vaccines should be considered a potent prime in prime-boost vaccination protocols."],["dc.identifier.doi","10.1128/JVI.01120-17"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85649"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1098-5514"],["dc.relation.issn","0022-538X"],["dc.title","Vector Order Determines Protection against Pathogenic Simian Immunodeficiency Virus Infection in a Triple-Component Vaccine by Balancing CD4 + and CD8 + T-Cell Responses"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","249"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Human Genetics"],["dc.bibliographiccitation.lastpage","254"],["dc.bibliographiccitation.volume","108"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Nürnberg, Peter"],["dc.contributor.author","Bercovitch, Fred"],["dc.contributor.author","Berard, John"],["dc.contributor.author","Trefilov, Andrea"],["dc.contributor.author","Widdig, Anja"],["dc.contributor.author","Kessler, Matt"],["dc.contributor.author","Schmidtke, Jörg"],["dc.contributor.author","Krawczak, Michael"],["dc.date.accessioned","2022-10-06T13:32:17Z"],["dc.date.available","2022-10-06T13:32:17Z"],["dc.date.issued","2001"],["dc.identifier.doi","10.1007/s004390100485"],["dc.identifier.pii","3P682096AWR61A34"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115335"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1432-1203"],["dc.relation.issn","0340-6717"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Increased reproductive success of MHC class II heterozygous males among free-ranging rhesus macaques"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3438"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","The Journal of Immunology"],["dc.bibliographiccitation.lastpage","3446"],["dc.bibliographiccitation.volume","169"],["dc.contributor.author","Mühl, Thorsten"],["dc.contributor.author","Krawczak, Michael"],["dc.contributor.author","ten Haaft, Peter"],["dc.contributor.author","Hunsmann, Gerhard"],["dc.contributor.author","Sauermann, Ulrike"],["dc.date.accessioned","2021-06-01T10:48:53Z"],["dc.date.available","2021-06-01T10:48:53Z"],["dc.date.issued","2002"],["dc.identifier.doi","10.4049/jimmunol.169.6.3438"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86087"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1550-6606"],["dc.relation.issn","0022-1767"],["dc.title","MHC Class I Alleles Influence Set-Point Viral Load and Survival Time in Simian Immunodeficiency Virus-Infected Rhesus Monkeys"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0172847"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","PLOS ONE"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Winkler, Michael"],["dc.contributor.author","Gärtner, Sabine"],["dc.contributor.author","Wrensch, Florian"],["dc.contributor.author","Krawczak, Michael"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.date.accessioned","2019-07-09T11:43:17Z"],["dc.date.available","2019-07-09T11:43:17Z"],["dc.date.issued","2017"],["dc.description.abstract","nterferon-induced transmembrane proteins (IFITMs) have been recognized as important antiviral effectors of the innate immune system, both in cell culture and in infected humans. In particular, polymorphisms of the human IFITM3 gene have been shown to affect disease severity and progression in influenza A virus (FLUAV) and human immunodeficiency virus (HIV) infection, respectively. Rhesus macaques (Macaca mulatta) are commonly used to model human infections and the experimental inoculation of these animals with simian immunodeficiency virus (SIV) is one of the best models for HIV/AIDS in humans. However, information on the role of IFITM3 in SIV infection of rhesus macaques is currently lacking. We show that rhesus macaque (rh) IFITM3 inhibits SIV and FLUAV entry in cell culture, although with moderately reduced efficiency as compared to its human counterpart. We further report the identification of 16 polymorphisms in the rhIFITM3 gene, three of which were exonic and synonymous while the remainder was located in non-coding regions. Employing previously characterized samples from two cohorts of SIV-infected rhesus macaques, we investigated the relationship between these rhIFITM3 polymorphisms and both AIDS-free survival time and virus load. In cohort 1, several intronic polymorphisms were significantly associated with virus load or survival. However, an association with both parameters was not observed and significance was lost in most cases when animals were stratified for the presence of MHC allele Mamu-A1 001. Moreover, no significant genotype-phenotype associations were detected in cohort 2. These results suggest that, although IFITM3 can inhibit SIV infection in cell culture, genetic variation in rhIFITM3 might have only a minor impact on the course of SIV infection in experimentally infected animals."],["dc.identifier.doi","10.1371/journal.pone.0172847"],["dc.identifier.pmid","28257482"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14379"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58850"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Rhesus macaque IFITM3 gene polymorphisms and SIV infection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","44"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","American Journal of Primatology"],["dc.bibliographiccitation.lastpage","62"],["dc.bibliographiccitation.volume","78"],["dc.contributor.author","Widdig, Anja"],["dc.contributor.author","Kessler, Matthew J."],["dc.contributor.author","Bercovitch, Fred B."],["dc.contributor.author","Berard, John D."],["dc.contributor.author","Duggleby, Christine"],["dc.contributor.author","Nürnberg, Peter"],["dc.contributor.author","Rawlins, Richard G."],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Wang, Qian"],["dc.contributor.author","Krawczak, Michael"],["dc.contributor.author","Schmidtke, Jörg"],["dc.date.accessioned","2022-10-06T13:33:43Z"],["dc.date.available","2022-10-06T13:33:43Z"],["dc.date.issued","2015"],["dc.identifier.doi","10.1002/ajp.22424"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115710"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.issn","0275-2565"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Genetic studies on the Cayo Santiago rhesus macaques: A review of 40 years of research"],["dc.title.alternative","Genetic Studies on the Cayo Santiago Rhesus Macaques"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","716"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","The Journal of Infectious Diseases"],["dc.bibliographiccitation.lastpage","724"],["dc.bibliographiccitation.volume","182"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Stolte, Nicole"],["dc.contributor.author","Mühl, Thorsten"],["dc.contributor.author","Krawczak, Michael"],["dc.contributor.author","Spring, Michael"],["dc.contributor.author","Fuchs, Dietmar"],["dc.contributor.author","Kaup, Franz-Josef"],["dc.contributor.author","Hunsmann, Gerhard"],["dc.contributor.author","Sopper, Sieghart"],["dc.date.accessioned","2022-10-06T13:34:38Z"],["dc.date.available","2022-10-06T13:34:38Z"],["dc.date.issued","2000"],["dc.identifier.doi","10.1086/315800"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115957"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1537-6613"],["dc.relation.issn","0022-1899"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Homozygosity for a Conserved Mhc Class II DQ-DRB Haplotype Is Associated with Rapid Disease Progression in Simian Immunodeficiency Virus—Infected Macaques: Results from a Prospective Study"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","819"],["dc.bibliographiccitation.issue","1541"],["dc.bibliographiccitation.journal","Proceedings of the Royal Society of London. Series B: Biological Sciences"],["dc.bibliographiccitation.lastpage","826"],["dc.bibliographiccitation.volume","271"],["dc.contributor.author","Widdig, Anja"],["dc.contributor.author","Bercovitch, Fred B."],["dc.contributor.author","Jürgen Streich, Wolf"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Nürnberg, Peter"],["dc.contributor.author","Krawczak, Michael"],["dc.date.accessioned","2022-10-06T13:35:35Z"],["dc.date.available","2022-10-06T13:35:35Z"],["dc.date.issued","2004"],["dc.identifier.doi","10.1098/rspb.2003.2666"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116132"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1471-2954"],["dc.relation.issn","0962-8452"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","A longitudinal analysis of reproductive skew in male rhesus macaques"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]