Hippe, Diana

[["dc.bibliographiccitation.firstpage","3511"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Journal of Cell Science"],["dc.bibliographiccitation.lastpage","3521"],["dc.bibliographiccitation.volume","122"],["dc.contributor.author","Hippe, Diana"],["dc.contributor.author","Weber, Arnim"],["dc.contributor.author","Zhou, L."],["dc.contributor.author","Chang, Donald C."],["dc.contributor.author","Haecker, Georg"],["dc.contributor.author","Lueder, Carsten Guenter Kurt"],["dc.date.accessioned","2018-11-07T11:23:48Z"],["dc.date.available","2018-11-07T11:23:48Z"],["dc.date.issued","2009"],["dc.description.abstract","In order to accomplish their life style, intracellular pathogens, including the apicomplexan Toxoplasma gondii, subvert the innate apoptotic response of infected host cells. However, the precise mechanisms of parasite interference with the mitochondrial apoptotic pathway remain unknown. Here, we used the conditional expression of the BH3-only protein Bim(S) to pinpoint the interaction of T. gondii with the intrinsic pathway of apoptosis. Infection of epithelial cells with T. gondii dose-dependently abrogated Bim(S)-triggered release of cytochrome c from host-cell mitochondria into the cytosol, induction of activity of caspases 3, 7 and 9, and chromatin condensation. Furthermore, inhibition of apoptosis in parasite-infected lymphocytes counteracted death of Toxoplasma-infected host cells. Although total cellular levels and mitochondrial targeting of BimS was not altered by the infection, the activation of pro-apoptotic effector proteins Bax and Bak was strongly impaired. Inhibition of Bax and Bak activation by T. gondii was seen with regard to their conformational changes, the cytosol-to-mitochondria targeting and the oligomerization of Bax but not their cellular protein levels. Blockade of Bax and Bak activation was not mediated by the upregulation of antiapoptotic Bcl-2-like proteins following infection. Further, the BH3-mimetic ABT-737 failed to overcome the Toxoplasma-imposed inhibition of Bim(S)-triggered apoptosis. These results indicate that T. gondii targets activation of pro-apoptotic Bax and Bak to inhibit the apoptogenic function of mitochondria and to increase host-cell viability."],["dc.identifier.doi","10.1242/jcs.050963"],["dc.identifier.isi","000269839900010"],["dc.identifier.pmid","19737817"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6225"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56267"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Company Of Biologists Ltd"],["dc.relation.issn","0021-9533"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Toxoplasma gondii infection confers resistance against Bim(S)-induced apoptosis by preventing the activation and mitochondrial targeting of pro-apoptotic Bax"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","105"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","106"],["dc.bibliographiccitation.volume","296"],["dc.contributor.author","Hippe, Diana"],["dc.contributor.author","Vutova, Polya"],["dc.contributor.author","Haecker, Georg"],["dc.contributor.author","Gross, U."],["dc.contributor.author","Lueder, Carsten Guenter Kurt"],["dc.date.accessioned","2018-11-07T09:19:40Z"],["dc.date.available","2018-11-07T09:19:40Z"],["dc.date.issued","2006"],["dc.identifier.isi","000241442600156"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28691"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","58th Annual Conference of the German-Society-of-Hygiene-and-Microbiology"],["dc.relation.eventlocation","Wurzburg, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Toxoplasma gondii inhibits host cell apoptosis triggered by both intrinsic and extrinsic proapoptotic signals"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","23"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","24"],["dc.bibliographiccitation.volume","297"],["dc.contributor.author","Hippe, Diana"],["dc.contributor.author","Weber, A."],["dc.contributor.author","Gross, U."],["dc.contributor.author","Haecker, Georg"],["dc.contributor.author","Lueder, Carsten Guenter Kurt"],["dc.date.accessioned","2018-11-07T10:59:03Z"],["dc.date.available","2018-11-07T10:59:03Z"],["dc.date.issued","2007"],["dc.identifier.isi","000250104800055"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50608"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","59th Annual Meeting of the Deutschen-Gesellschaft-fur-Hygiene-und-Mikrobiologie"],["dc.relation.eventlocation","Gottingen, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Toxoplasma gondii inhibits activation and oligomerisation of proapoptotic Bax during Bims-induced apoptosis"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]