Arakel, Eric Clement

[["dc.bibliographiccitation.firstpage","672"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Traffic"],["dc.bibliographiccitation.lastpage","682"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Geva, Yosef"],["dc.contributor.author","Crissman, Jonathan"],["dc.contributor.author","Arakel, Eric C."],["dc.contributor.author","Gómez-Navarro, Natalia"],["dc.contributor.author","Chuartzman, Silvia G."],["dc.contributor.author","Stahmer, Kyle R."],["dc.contributor.author","Schwappach, Blanche"],["dc.contributor.author","Miller, Elizabeth A."],["dc.contributor.author","Schuldiner, Maya"],["dc.date.accessioned","2018-04-23T11:49:04Z"],["dc.date.available","2018-04-23T11:49:04Z"],["dc.date.issued","2017"],["dc.description.abstract","The endoplasmic reticulum (ER) is the entry site of proteins into the endomembrane system. Proteins exit the ER via coat protein II (COPII) vesicles in a selective manner, mediated either by direct interaction with the COPII coat or aided by cargo receptors. Despite the fundamental role of such receptors in protein sorting, only a few have been identified. To further define the machinery that packages secretory cargo and targets proteins from the ER to Golgi membranes, we used multiple systematic approaches, which revealed 2 uncharacterized proteins that mediate the trafficking and maturation of Pma1, the essential yeast plasma membrane proton ATPase. Ydl121c (Exp1) is an ER protein that binds Pma1, is packaged into COPII vesicles, and whose deletion causes ER retention of Pma1. Ykl077w (Psg1) physically interacts with Exp1 and can be found in the Golgi and coat protein I (COPI) vesicles but does not directly bind Pma1. Loss of Psg1 causes enhanced degradation of Pma1 in the vacuole. Our findings suggest that Exp1 is a Pma1 cargo receptor and that Psg1 aids Pma1 maturation in the Golgi or affects its retrieval. More generally our work shows the utility of high content screens in the identification of novel trafficking components."],["dc.identifier.doi","10.1111/tra.12503"],["dc.identifier.gro","3142485"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13637"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/11"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P04: Der GET-Rezeptor als ein Eingangstor zum ER und sein Zusammenspiel mit GET bodies"],["dc.relation","SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel"],["dc.relation.issn","1398-9219"],["dc.relation.workinggroup","RG Schuldiner (Functional Genomics of Organelles)"],["dc.relation.workinggroup","RG Schwappach (Membrane Protein Biogenesis)"],["dc.rights","CC BY 4.0"],["dc.title","Two novel effectors of trafficking and maturation of the yeast plasma membrane H+-ATPase"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","overview_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","370"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Traffic"],["dc.bibliographiccitation.lastpage","379"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Weill, Uri"],["dc.contributor.author","Arakel, Eric C."],["dc.contributor.author","Goldmann, Omer"],["dc.contributor.author","Golan, Matan"],["dc.contributor.author","Chuartzman, Silvia"],["dc.contributor.author","Munro, Sean"],["dc.contributor.author","Schwappach, Blanche"],["dc.contributor.author","Schuldiner, Maya"],["dc.date.accessioned","2020-12-10T18:36:31Z"],["dc.date.available","2020-12-10T18:36:31Z"],["dc.date.issued","2018"],["dc.description.abstract","A third of yeast genes encode for proteins that function in the endomembrane system. However, the precise localization for many of these proteins is still uncertain. Here, we visualized a collection of ~500 N-terminally, green fluorescent protein (GFP), tagged proteins of the yeast Saccharomyces cerevisiae. By co-localizing them with 7 known markers of endomembrane compartments we determined the localization for over 200 of them. Using this approach, we create a systematic database of the various secretory compartments and identify several new residents. Focusing in, we now suggest that Lam5 resides in contact sites between the endoplasmic reticulum and the late Golgi. Additionally, analysis of interactions between the COPI coat and co-localizing proteins from our screen identifies a subset of proteins that are COPI-cargo. In summary, our approach defines the protein roster within each compartment enabling characterization of the physical and functional organization of the endomembrane system and its components."],["dc.identifier.doi","10.1111/tra.12560"],["dc.identifier.pmid","29527758"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76654"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/24"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P04: Der GET-Rezeptor als ein Eingangstor zum ER und sein Zusammenspiel mit GET bodies"],["dc.relation","SFB 1190 | P11: Zuordnung zellulärer Kontaktstellen und deren Zusammenspiel"],["dc.relation","SFB 1190 | Z03: Synthetische genetische Analyse, automatisierte Mikroskopie und Bildanalyse"],["dc.relation.issn","1398-9219"],["dc.relation.workinggroup","RG Schuldiner (Functional Genomics of Organelles)"],["dc.relation.workinggroup","RG Schwappach (Membrane Protein Biogenesis)"],["dc.rights","CC BY 4.0"],["dc.title","Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]