Dressel, Ralf

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Tissue Antigens"],["dc.bibliographiccitation.volume","84"],["dc.contributor.author","Monecke, Sebastian"],["dc.contributor.author","Hamann, Carina"],["dc.contributor.author","Elsner, Leslie"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Mansouri, Ahmed"],["dc.contributor.author","Dressel, Ralf"],["dc.date.accessioned","2018-11-07T09:38:32Z"],["dc.date.available","2018-11-07T09:38:32Z"],["dc.date.issued","2014"],["dc.format.extent","5"],["dc.identifier.isi","000337546000002"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33083"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.eventlocation","Stockholm, SWEDEN"],["dc.relation.issn","1399-0039"],["dc.relation.issn","0001-2815"],["dc.title","PLURIPOTENT STEM CELLS VARYING IN A SINGLE MINOR HISTOCOMPATIBILITY ANTIGEN ELICIT CELLULAR AND HUMORAL IMMUNE RESPONSES THAT CAN MEDIATE GRAFT REJECTION"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1199"],["dc.bibliographiccitation.issue","7088"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","1203"],["dc.bibliographiccitation.volume","440"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Maier, Lars S."],["dc.contributor.author","Wagner, Stefan"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Lee, Jae Ho"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Wolf, F"],["dc.contributor.author","Li, M. Y."],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.date.accessioned","2017-09-07T11:53:07Z"],["dc.date.available","2017-09-07T11:53:07Z"],["dc.date.issued","2006"],["dc.description.abstract","Embryonic germ cells as well as germline stem cells from neonatal mouse testis are pluripotent and have differentiation potential similar to embryonic stem cells(1,2), suggesting that the germline lineage may retain the ability to generate pluripotent cells. However, until now there has been no evidence for the pluripotency and plasticity of adult spermatogonial stem cells (SSCs), which are responsible for maintaining spermatogenesis throughout life in the male(3). Here we show the isolation of SSCs from adult mouse testis using genetic selection, with a success rate of 27%. These isolated SSCs respond to culture conditions and acquire embryonic stem cell properties. We name these cells multipotent adult germline stem cells (maGSCs). They are able to spontaneously differentiate into derivatives of the three embryonic germ layers in vitro and generate teratomas in immunodeficient mice. When injected into an early blastocyst, SSCs contribute to the development of various organs and show germline transmission. Thus, the capacity to form multipotent cells persists in adult mouse testis. Establishment of human maGSCs from testicular biopsies may allow individual cell-based therapy without the ethical and immunological problems associated with human embryonic stem cells. Furthermore, these cells may provide new opportunities to study genetic diseases in various cell lineages."],["dc.identifier.doi","10.1038/nature04697"],["dc.identifier.gro","3143702"],["dc.identifier.isi","000237080000047"],["dc.identifier.pmid","16565704"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1245"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0028-0836"],["dc.title","Pluripotency of spermatogonial stem cells from adult mouse testis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","166"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","MHR Basic science of reproductive medicine"],["dc.bibliographiccitation.lastpage","174"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Khromov, Tatjana"],["dc.contributor.author","Pantakani, Dasaradha Venkata Krishna"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Wolf, Marieke"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Zechner, Ulrich"],["dc.date.accessioned","2018-11-07T08:58:57Z"],["dc.date.available","2018-11-07T08:58:57Z"],["dc.date.issued","2011"],["dc.description.abstract","We previously reported the generation of multipotent adult germline stem cells (maGSCs) from spermatogonial stem cells (SSCs) isolated from adult mouse testis. In a later study, we substantiated the pluripotency of maGSCs by demonstrating their close similarity to pluripotent male embryonic stem cells (ESCs) at the epigenetic level of global and gene-specific DNA methylation. Here, we extended the comparative epigenetic analysis of maGSCs and male ESCs by investigating the second main epigenetic modification in mammals, i.e. global and gene-specific modifications of histones (H3K4 trimethylation, H3K9 acetylation, H3K9 trimethylation and H3K27 trimethylation). Using immunofluorescence staining, flow cytometry and western blot analysis, we show that maGSCs are very similar to male ESCs with regard to global levels and nuclear distribution patterns of these modifications. Chromatin immunoprecipitation real-time PCR analysis of these modifications at the gene-specific level further revealed modification patterns of the pluripotency marker genes Oct4, Sox2 and Nanog in maGSCs that are nearly identical to those of male ESCs. These genes were enriched for activating histone modifications including H3K4me3 and H3K9ac and depleted of repressive histone modifications including H3K27me3 and H3K9me3. In addition, Hoxa11, a key regulator of early embryonic development showed the ESC-typical bivalent chromatin conformation with enrichment of both the activating H3K4me3 and the repressive H3K27me3 modification also in maGSCs. Collectively, our results demonstrate that maGSCs also closely resemble ESCs with regard to their chromatin state and further evidence their pluripotent nature."],["dc.identifier.doi","10.1093/molehr/gaq085"],["dc.identifier.isi","000287257000003"],["dc.identifier.pmid","20935159"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23771"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1360-9947"],["dc.title","Global and gene-specific histone modification profiles of mouse multipotent adult germline stem cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","16"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cytogenetic and Genome Research"],["dc.bibliographiccitation.lastpage","26"],["dc.bibliographiccitation.volume","111"],["dc.contributor.author","Adham, Ibrahim M."],["dc.contributor.author","Gille, M."],["dc.contributor.author","Gamel, A. J."],["dc.contributor.author","Reis, A."],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Steding, G."],["dc.contributor.author","Brand-Saberi, B."],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T08:37:05Z"],["dc.date.available","2018-11-07T08:37:05Z"],["dc.date.issued","2005"],["dc.description.abstract","We describe the spontaneous mutant mouse scoliosis (sco) that carries a new allele of Pax1 (un-i, undulated intermediate). The pax1(un-i) allele is lacking the 5'-flanking region and exon 1 to 4 which is mapped to nt -2636 to -640 and -272 to 4271 of the Pax1 gene. Homozygous mice show a mild form of the known phenotypes of other Pax] mutants. Adult mice have a lumbar scoliosis and kinky tails. In homozygous embryos the skeleton ossifies early, ossification centers of the vertebral bodies are fused with the ossification centers of the pedicles. Neural arches and spinous processes are underdeveloped but the pedicles and transverse processes are overdeveloped which is in contrast to other Pax] mutants. In the scapula, the acromion is missing and the deltoid tuberosity of the proximal humerus is shortened and thickened. Among the inner organs the thymus development is affected. In late embryos, the thymus is small and thymocyte numbers are reduced. T-cell development from CD4- and CD8- double negative (DN) to CD4(+) and CD8(+) double positive (DP) is decelerated. The percentage of CD90(+) cells is also reduced but in contrast to other Pax] mutants no alteration of the expression level of the CD90 (Thy-1) could be found. Copyright (c) 2005S. KargerAG, Base."],["dc.identifier.doi","10.1159/000085665"],["dc.identifier.isi","000231728300003"],["dc.identifier.pmid","16093716"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18447"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.relation.issn","1424-8581"],["dc.title","The scoliosis (sco)mouse: a new allele of Pax1"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","394"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Tissue Antigens"],["dc.bibliographiccitation.lastpage","395"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Elsner, Leslie"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T08:30:35Z"],["dc.date.available","2018-11-07T08:30:35Z"],["dc.date.issued","2009"],["dc.identifier.isi","000266032200026"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16928"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.publisher.place","Malden"],["dc.relation.eventlocation","Ulm, GERMANY"],["dc.relation.issn","0001-2815"],["dc.title","Multipotent adult germline stem cells and embryonic stem cells are targets for cytotoxic T lymphocytes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1664"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Journal of Investigative Dermatology"],["dc.bibliographiccitation.lastpage","1671"],["dc.bibliographiccitation.volume","136"],["dc.contributor.author","Elkenani, Manar"],["dc.contributor.author","Nyamsuren, Gunsmaa"],["dc.contributor.author","Raju, Priyadharsini"],["dc.contributor.author","Liakath-Ali, Kifayathullah"],["dc.contributor.author","Hamdaoui, Aicha"],["dc.contributor.author","Kata, Aleksandra"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Klonisch, Thomas"],["dc.contributor.author","Watt, Fiona M."],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Thliveris, James A."],["dc.contributor.author","Pantakani, Dasaradha Venkata Krishna"],["dc.contributor.author","Adham, Ibrahim M."],["dc.date.accessioned","2018-11-07T10:11:05Z"],["dc.date.available","2018-11-07T10:11:05Z"],["dc.date.issued","2016"],["dc.description.abstract","The depletion of evolutionarily conserved pelota protein causes impaired differentiation of embryonic and spermatogonial stem cells. In this study, we show that temporal deletion of pelota protein before epidermal barrier acquisition leads to neonatal lethality due to perturbations in permeability barrier formation. Further analysis indicated that this phenotype is a result of failed processing of profilaggrin into filaggrin monomers, which promotes the formation of a protective epidermal layer. Molecular analyses showed that pelota protein negatively regulates the activities of bone morphogenetic protein and phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathways in the epidermis. To address whether elevated activities of bone morphogenetic protein and PI3K/AKT signaling pathways were the cause for the perturbed epidermal barrier in Pelo-deficient mice, we made use of organotypic cultures of skin explants from control and mutant embryos at embryonic day 15.5. Inhibition of PI3K/AKT signaling did not significantly affect the bone morphogenetic protein activity. However, inhibition of bone morphogenetic protein signaling caused a significant attenuation of PI3K/AKT activity in mutant skin and, more interestingly, the restoration of profilaggrin processing and normal epidermal barrier function. Therefore, increased activity of the PI3K/AKT signaling pathway in Pelo-deficient skin might conflict with the dephosphorylation of profilaggrin and thereby affect its proper processing into filaggrin monomers and ultimately the epidermal differentiation."],["dc.description.sponsorship","Medical Research Council [G1100073, MR/L022699/1]"],["dc.identifier.doi","10.1016/j.jid.2016.04.020"],["dc.identifier.isi","000380585200092"],["dc.identifier.pmid","27164299"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39976"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","1523-1747"],["dc.relation.issn","0022-202X"],["dc.title","Pelota Regulates Epidermal Differentiation by Modulating BMP and PI3K/AKT Signaling Pathways"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","654"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Laboratory Investigation"],["dc.bibliographiccitation.lastpage","663"],["dc.bibliographiccitation.volume","86"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Lee, Jae Ho"],["dc.contributor.author","Drusenheimer, Nadja"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Wulf, Gerald"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Gromoll, Joerg"],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T09:37:44Z"],["dc.date.available","2018-11-07T09:37:44Z"],["dc.date.issued","2006"],["dc.description.abstract","Recent studies have demonstrated that somatic stem cells have a more flexible potential than expected, whether put into tissue or cultured under different conditions. Bone marrow (BM)-derived stem cells can transdifferentiate into multilineage cells, such as muscle of mesoderm, lung and liver of endoderm, and brain and skin of ectoderm origin. Here we show that BM stem cells are able to transdifferentiate into male germ cells. For derivation of male germ cells from adult BM stem (BMS) cells, we used the Stra8-enhanced green fluoresence protein (EGFP) transgenic mouse line expressing EGFP specifically in male germ cells. BMS cell-derived germ cells expressed the known molecular markers of primordial germ cells, such as fragilis, stella, Rnf17, Mvh and Oct4; as well as molecular markers of spermatogonial stem cells and spermatogonia including Rbm, c-Kit, Tex18, Stra8, Piwil2, Dazl, Hsp90 alpha, beta 1- and alpha 6-integrins. Our ability to derive male germ cells from BMS cells reveals novel aspects of germ cell development and opens the possibilities for use of these cells in reproductive medicine."],["dc.identifier.doi","10.1038/labinvest.3700429"],["dc.identifier.isi","000238312000004"],["dc.identifier.pmid","16652109"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32902"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","0023-6837"],["dc.title","Derivation of male germ cells from bone marrow stem cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]