Gregor, Ingo

[["dc.bibliographiccitation.firstpage","e3000943"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","PLoS Biology"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Trevisiol, Andrea"],["dc.contributor.author","Kusch, Kathrin"],["dc.contributor.author","Steyer, Anna M."],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Nardis, Christos"],["dc.contributor.author","Winkler, Ulrike"],["dc.contributor.author","Köhler, Susanne"],["dc.contributor.author","Restrepo, Alejandro"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Werner, Hauke B."],["dc.contributor.author","Nave, Klaus-Armin"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.date.accessioned","2021-04-14T08:31:16Z"],["dc.date.available","2021-04-14T08:31:16Z"],["dc.date.issued","2020"],["dc.description.abstract","In several neurodegenerative disorders, axonal pathology may originate from impaired oligodendrocyte-to-axon support of energy substrates. We previously established transgenic mice that allow measuring axonal ATP levels in electrically active optic nerves. Here, we utilize this technique to explore axonal ATP dynamics in the Plpnull/y mouse model of spastic paraplegia. Optic nerves from Plpnull/y mice exhibited lower and more variable basal axonal ATP levels and reduced compound action potential (CAP) amplitudes, providing a missing link between axonal pathology and a role of oligodendrocytes in brain energy metabolism. Surprisingly, when Plpnull/y optic nerves are challenged with transient glucose deprivation, both ATP levels and CAP decline slower, but recover faster upon reperfusion of glucose. Structurally, myelin sheaths display an increased frequency of cytosolic channels comprising glucose and monocarboxylate transporters, possibly facilitating accessibility of energy substrates to the axon. These data imply that complex metabolic alterations of the axon–myelin unit contribute to the phenotype of Plpnull/y mice."],["dc.identifier.doi","10.1371/journal.pbio.3000943"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83539"],["dc.identifier.url","https://for2848.gwdguser.de/literature/publications/20"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","FOR 2848: Architektur und Heterogenität der inneren mitochondrialen Membran auf der Nanoskala"],["dc.relation","FOR 2848 | P08: Strukturelle und funktionale Veränderungen der inneren mitochondrialen Membran axonaler Mitochondrien in vivo in einem dymyelinisierenden Mausmodell"],["dc.relation.eissn","1545-7885"],["dc.relation.workinggroup","RG Möbius"],["dc.rights","CC BY 4.0"],["dc.title","Structural myelin defects are associated with low axonal ATP levels but rapid recovery from energy deprivation in a mouse model of spastic paraplegia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Communications Biology"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Jensen, Nickels"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Chizhik, Alexey I."],["dc.date.accessioned","2021-03-05T08:58:32Z"],["dc.date.available","2021-03-05T08:58:32Z"],["dc.date.issued","2020"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1038/s42003-020-01316-2"],["dc.identifier.pmid","33128009"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17780"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80175"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/87"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.notes.intern","Merged from goescholar"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2399-3642"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Enderlein"],["dc.relation.workinggroup","RG Jakobs (Structure and Dynamics of Mitochondria)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Absolute quantum yield measurements of fluorescent proteins using a plasmonic nanocavity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","885"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","899"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Stefl, Martin"],["dc.contributor.author","Benda, Ales"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Hof, Martin"],["dc.date.accessioned","2018-11-07T09:45:09Z"],["dc.date.available","2018-11-07T09:45:09Z"],["dc.date.issued","2014"],["dc.description.abstract","We introduce two new alternative experimental realizations of dual focus fluorescence correlation spectroscopy (2fFCS), a method which allows for obtaining absolute diffusion coefficient of fast moving fluorescing molecules at nanomolar concentrations, based on fast polarization modulation of the excitation beam by a resonant electro-optical modulator. The first approach rotates every second linearly polarized laser pulse by 90 degrees to obtain independent intensity readout for both foci, similar to original design. The second approach combines polarization modulation of cw laser and fluorescence lifetime correlation spectroscopy (FLCS) like analysis to obtain clean correlation curves for both overlapping foci. We tested our new approaches with different lasers and samples, revealed a need for intensity cross-talk corrections by comparing the methods with each other and discussed experimental artifacts stemming from improper polarization alignment and detector afterpulsing. The advantages of our solutions are that the polarization rotation approach requires just one pulsed laser for each wavelength, that the polarization modulation approach even mitigates the need of pulsed lasers by using standard cw lasers and that it allows the DIC prism to be placed at an arbitrary angle. As a consequence the presented experimental solutions for 2fFCS can be more easily implemented into commercial laser scanning microscopes. (C) 2014 Optical Society of America"],["dc.identifier.doi","10.1364/OE.22.000885"],["dc.identifier.isi","000330579300110"],["dc.identifier.pmid","24515048"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12634"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34555"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Optical Soc Amer"],["dc.relation.issn","1094-4087"],["dc.relation.orgunit","Fakultät für Physik"],["dc.subject.mesh","Algorithms"],["dc.subject.mesh","Equipment Design"],["dc.subject.mesh","Equipment Failure Analysis"],["dc.subject.mesh","Molecular Imaging"],["dc.subject.mesh","Refractometry"],["dc.subject.mesh","Spectrometry, Fluorescence"],["dc.title","The fast polarization modulation based dualfocus fluorescence correlation spectroscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","8011"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","8018"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Ruckstuhl, Thomas"],["dc.date.accessioned","2021-03-05T08:59:09Z"],["dc.date.available","2021-03-05T08:59:09Z"],["dc.date.issued","2011"],["dc.description.abstract","In recent years, new optical systems have been developed with the ability to collect light at very high angles of emission, exceeding the critical angle of total internal reflection. Prominent examples are solid-immersion lenses and paraboloid collectors. These systems achieve high efficiencies in fluorescence detection which is an important issue for sensitive applications in analytical chemistry and biochemical assays. The exclusive collection of supercritical angle fluorescence (SAF) allows for the detection of evanescent modes and thus to confine the detection volume within one wavelength to an interface. For conventional optical systems with high numerical aperture a precise wave-optical theory of imaging was developed by Richards and Wolf in the fifties of the last century. However, their theory is not directly applicable to non-imaging, strongly aberratic light collection systems systems that collect a significant part of light above the critical angle. Here, we extend the theory to describe the optical properties of such systems."],["dc.identifier.doi","10.1364/OE.19.008011"],["dc.identifier.fs","590727"],["dc.identifier.gro","3142140"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8629"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80373"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.doi","10.1364/oe.19.008011"],["dc.relation.eissn","1094-4087"],["dc.relation.issn","1094-4087"],["dc.relation.issn","1094-4087"],["dc.relation.orgunit","Fakultät für Physik"],["dc.title","Imaging properties of supercritical angle fluorescence optics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1689"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Nanophotonics"],["dc.bibliographiccitation.lastpage","1699"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Chizhik, Alexey"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2020-12-10T18:42:27Z"],["dc.date.available","2020-12-10T18:42:27Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1515/nanoph-2019-0201"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16521"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77956"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/12"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Metal-induced energy transfer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9429"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","9445"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Isbaner, Sebastian"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Stein, Simon Christoph"],["dc.contributor.author","Chizhik, Anna"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2021-11-22T14:31:36Z"],["dc.date.available","2021-11-22T14:31:36Z"],["dc.date.issued","2016"],["dc.description.abstract","We present a comprehensive theory of dead-time effects on Time-Correlated Single Photon Counting (TCSPC) as used for fluorescence lifetime measurements, and develop a correction algorithm to remove these artifacts. We apply this algorithm to fluorescence lifetime measurements as well as to Fluorescence Lifetime Imaging Microscopy (FLIM), where rapid data acquisition is necessarily connected with high count rates. There, dead-time effects cannot be neglected, and lead to distortions in the observed lifetime image. The algorithm is quite general and completely independent of the particular nature of the measured signal. It can also be applied to any other single-event counting measurement with detector and/or electronics dead-time."],["dc.identifier.doi","10.1364/OE.24.009429"],["dc.identifier.gro","3142106"],["dc.identifier.pmid","27137558"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14123"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/93392"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.notes.status","final"],["dc.relation.issn","1094-4087"],["dc.rights.access","openAccess"],["dc.subject","fluorescence; lifetime; imaging"],["dc.title","Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","14994"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","Physical Chemistry Chemical Physics"],["dc.bibliographiccitation.lastpage","15000"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Tarpani, Luigi"],["dc.contributor.author","Latterini, Loredana"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Chizhik, Alexey I."],["dc.date.accessioned","2015-12-09T10:45:34Z"],["dc.date.accessioned","2021-10-11T11:34:51Z"],["dc.date.available","2015-12-09T10:45:34Z"],["dc.date.available","2021-10-11T11:34:51Z"],["dc.date.issued","2015"],["dc.description.abstract","We present the results of a comprehensive photoluminescence study of defect centres in single SiO2 nanoparticles. We show that the photo-physical properties of the luminescent centres strongly resemble those of single dye molecules. However, these properties exhibit a large variability from particle to particle due to the different local chemical environment around each centre of each particle. This variability provides new insight into the complex photo-physics of single quantum emitters embedded into a random chemical environment. Moreover, a better understanding of the fundamental mechanism of the photoluminescence of defect centres in SiO2 structure is paramount for their application as white-light sources, non-toxic labels for bio-imaging, or for combining them with metallic and semiconductor nanostructures."],["dc.identifier.citation","Chizhik, Anna M; Tarpani, Luigi; Latterini, Loredana; Gregor, Ingo; Enderlein, Jörg; Chizhik, Alexey I (2015): Photoluminescence of a single quantum emitter in a strongly inhomogeneous chemical environment. - Physical chemistry chemical physics : PCCP, Vol. 17, Nr. 22, p. 14994-5000"],["dc.identifier.doi","10.1039/c5cp01371b"],["dc.identifier.pmid","25986857"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12607"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/90707"],["dc.language","eng"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.notes.status","final"],["dc.relation.doi","10.1039/C5CP01371B"],["dc.relation.eissn","1463-9084"],["dc.relation.issn","1463-9084"],["dc.relation.issn","1463-9076"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 3.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0/"],["dc.subject","Photoluminescence; quantum emitter; inhomogeneous chemical environment"],["dc.title","Photoluminescence of a single quantum emitter in a strongly inhomogeneous chemical environment"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","15542"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Solovyev, Ilya D."],["dc.contributor.author","Gavshina, Alexandra V."],["dc.contributor.author","Katti, Aditya S."],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Vinokurov, Leonid M."],["dc.contributor.author","Lapshin, Grigory D."],["dc.contributor.author","Ivashina, Tatiana V."],["dc.contributor.author","Khrenova, Maria G."],["dc.contributor.author","Kireev, Igor I."],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Savitsky, Alexander P."],["dc.date.accessioned","2019-07-09T11:50:56Z"],["dc.date.available","2019-07-09T11:50:56Z"],["dc.date.issued","2018"],["dc.description.abstract","Photoconvertible fluorescent proteins (PCFPs) are widely used as markers for the visualization of intracellular processes and for sub-diffraction single-molecule localization microscopy. Although wild type of a new photoconvertible fluorescent protein SAASoti tends to aggregate, we succeeded, via rational mutagenesis, to obtain variants that formed either tetramers or monomers. We compare two approaches: one is based on the structural similarity between SAASoti and Kaede, which helped us to identify a single point mutation (V127T) at the protein's hydrophobic interface that leads to monomerization. The other is based on a chemical modification of amino groups of SAASoti with succinic anhydride, which converts the protein aggregates into monomers. Mass-spectrometric analysis helped us to identify that the modification of a single ε-amino group of lysine K145 in the strongly charged interface AB was sufficient to convert the protein into its tetrameric form. Furthermore, site-directed mutagenesis was used to generate mutants that proved to be either monomeric or tetrameric, both capable of rapid green-to-red photoconversion. This allows SAASoti to be used as a photoconvertible fluorescent marker for in vivo cell studies."],["dc.identifier.doi","10.1038/s41598-018-33250-z"],["dc.identifier.pmid","30341334"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16025"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59853"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.subject.ddc","530"],["dc.title","Monomerization of the photoconvertible fluorescent protein SAASoti by rational mutagenesis of single amino acids"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]