Schütz, Ekkehard

[["dc.bibliographiccitation.artnumber","e0154602"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Schuetz, Ekkehard"],["dc.contributor.author","Wehrhahn, Christin"],["dc.contributor.author","Wanjek, Marius"],["dc.contributor.author","Bortfeld, Ralf"],["dc.contributor.author","Wemheuer, Wilhelm E."],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Brenig, Bertram"],["dc.date.accessioned","2018-11-07T10:15:21Z"],["dc.date.available","2018-11-07T10:15:21Z"],["dc.date.issued","2016"],["dc.description.abstract","Background With the availability of massive SNP data for several economically important cattle breeds, haplotype tests have been performed to identify unknown recessive disorders. A number of so-called lethal haplotypes, have been uncovered in Holstein Friesian cattle and, for at least seven of these, the causative mutations have been identified in candidate genes. However, several lethal haplotypes still remain elusive. Here we report the molecular genetic causes of lethal haplotype 5 (HH5) and cholesterol deficiency (CDH). A targeted enrichment for the known genomic regions, followed by massive parallel sequencing was used to interrogate for causative mutations in a case/control approach. Methods Targeted enrichment for the known genomic regions, followed by massive parallel sequencing was used in a case/control approach. PCRs for the causing mutations were developed and compared to routine imputing in 2,100 (HH5) and 3,100 (CDH) cattle. Results HH5 is caused by a deletion of 138kbp, spanning position 93,233kb to 93,371kb on chromosome 9 (BTA9), harboring only dimethyl-adenosine transferase 1 (TFB1M). The deletion breakpoints are flanked by bovine long interspersed nuclear elements Bov-B (upstream) and L1ME3 (downstream), suggesting a homologous recombination/deletion event. TFB1M di-methylates adenine residues in the hairpin loop at the 3'-end of mitochondrial 12S rRNA, being essential for synthesis and function of the small ribosomal subunit of mitochondria. Homozygous TFB1M(-/-) mice reportedly exhibit embryonal lethality with developmental defects. A 2.8% allelic frequency was determined for the German HF population. CDH results from a 1.3kbp insertion of an endogenous retrovirus (ERV2-1-LTR_BT) into exon 5 of the APOB gene at BTA11: 77,959kb. The insertion is flanked by 6bp target site duplications as described for insertions mediated by retroviral integrases. A premature stop codon in the open reading frame of APOB is generated, resulting in a truncation of the protein to a length of only < 140 amino acids. Such early truncations have been shown to cause an inability of chylomicron excretion from intestinal cells, resulting in malabsorption of cholesterol. The allelic frequency of this mutation in the German HF population was 6.7%, which is substantially higher than reported so far. Compared to PCR assays inferring the genetic variants directly, the routine imputing used so far showed a diagnostic sensitivity of as low as 91% (HH5) and 88% (CDH), with a high specificity for both (>= 99.7%). Conclusion With the availability of direct genetic tests it will now be possible to more effectively reduce the carrier frequency and ultimately eliminate the disorders from the HF populations. Beside this, the fact that repetitive genomic elements (RE) are involved in both diseases, underline the evolutionary importance of RE, which can be detrimental as here, but also advantageous over generations."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.1371/journal.pone.0154602"],["dc.identifier.isi","000375212600046"],["dc.identifier.pmid","27128314"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13249"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40795"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights.access","openAccess"],["dc.title","The Holstein Friesian Lethal Haplotype 5 (HH5) Results from a Complete Deletion of TBF1M and Cholesterol Deficiency (CDH) from an ERV-(LTR) Insertion into the Coding Region of APOB"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","550"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.lastpage","556"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Gordon, Paul M. K."],["dc.contributor.author","Schuetz, Ekkehard"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Urnovitz, Howard B."],["dc.contributor.author","Graham, Catherine"],["dc.contributor.author","Clark, Renee"],["dc.contributor.author","Dudas, Sandor"],["dc.contributor.author","Czub, Stefanie"],["dc.contributor.author","Sensen, Maria"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Groschup, Martin H."],["dc.contributor.author","Church, Robert B."],["dc.contributor.author","Sensen, Christoph W."],["dc.date.accessioned","2018-11-07T08:33:15Z"],["dc.date.available","2018-11-07T08:33:15Z"],["dc.date.issued","2009"],["dc.description.abstract","To gain insight into the disease progression of transmissible spongiform encephalopathies (TSE), we searched for disease-specific patterns in circulating nucleic acids (CNA) in elk and cattle. In a 25-month time-course experiment, CNAs were isolated from blood samples of 24 elk (Cervus elaphus) orally challenged with chronic wasting disease (CWD) infectious material. In a separate experiment, blood-sample CNAs from 29 experimental cattle (Bos taurus) 40 months post-inoculation with clinical bovine spongiform encephalopathy (BSE) were analyzed according to the same protocol. Next-generation sequencing provided broad elucidation of sample CNAs: we detected infection-specific sequences as early as 11 months in elk (i.e. at least 3 months before the appearance of the first clinical signs) and we established CNA patterns related to BSE in cattle at least 4 months prior to clinical signs. In elk, a progression of CNA sequence patterns was found to precede and correlate with macro-observable disease progression, including delayed CWD progression in elk with PrP genotype LM. Some of the patterns identified contain transcription-factor-binding sites linked to endogenous retroviral integration. These patterns suggest that retroviruses may be connected to the manifestation of TSEs. Our results may become useful for the early diagnosis of TSE in live elk and cattle."],["dc.identifier.doi","10.1093/nar/gkn963"],["dc.identifier.isi","000262963400031"],["dc.identifier.pmid","19059996"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4005"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17531"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","0305-1048"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Disease-specific motifs can be identified in circulating nucleic acids from live elk and cattle infected with transmissible spongiform encephalopathies"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e75485"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Hennecke, Silvia"],["dc.contributor.author","Bornemann-Kolatzki, Kirsten"],["dc.contributor.author","Urnovitz, Howard B."],["dc.contributor.author","Neumann, Stephan"],["dc.contributor.author","Stroebel, Philipp"],["dc.contributor.author","Kaup, Franz-Josef"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Schuetz, Ekkehard"],["dc.date.accessioned","2018-11-07T09:19:45Z"],["dc.date.available","2018-11-07T09:19:45Z"],["dc.date.issued","2013"],["dc.description.abstract","Mammary tumors are the most frequent cancers in female dogs exhibiting a variety of histopathological differences. There is lack of knowledge about the genomes of these common dog tumors. Five tumors of three different histological subtypes were evaluated. Massive parallel sequencing (MPS) was performed in comparison to the respective somatic genome of each animal. Copy number and structural aberrations were validated using droplet digital PCR (ddPCR). Using mate-pair sequencing chromosomal aneuploidies were found in two tumors, frequent smaller deletions were found in one, inter-chromosomal fusions in one other, whereas one tumor was almost normal. These aberrations affect several known cancer associated genes such as cMYC, and KIT. One common deletion of the proximal end of CFA27, harboring the tumor suppressor gene PFDN5 was detected in four tumors. Using ddPCR, this deletion was validated and detected in 50% of tumors (N = 20). Breakpoint specific dPCRs were established for four tumors and tumor specific cell-free DNA (cfDNA) was detected in the plasma. In one animal tumor-specific cfDNA was found >1 year after surgery, attributable to a lung metastasis. Paired-end sequencing proved that copy-number imbalances of the tumor are reflected by the cfDNA. This report on chromosomal instability of canine mammary cancers reveals similarities to human breast cancers as well as special canine alterations. This animal model provides a framework for using MPS for screening for individual cancer biomarkers with cost effective confirmation and monitoring using ddPCR. The possibility exists that ddPCR can be expanded to screening for common cancer related variants."],["dc.identifier.doi","10.1371/journal.pone.0075485"],["dc.identifier.isi","000325423500069"],["dc.identifier.pmid","24098698"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9419"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28716"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Genome Aberrations in Canine Mammary Carcinomas and Their Detection in Cell-Free Plasma DNA"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","228"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Forensic Science International Genetics"],["dc.bibliographiccitation.lastpage","231"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Beck, J."],["dc.contributor.author","Schuetz, Eckehardt"],["dc.date.accessioned","2018-11-07T08:41:39Z"],["dc.date.available","2018-11-07T08:41:39Z"],["dc.date.issued","2010"],["dc.description.abstract","A detailed molecular analysis of blood or other biological stains at a crime scene is often hampered by the low quantity and quality of the extractable DNA. However, the determination of the origin and composition of a stain is in most cases a prerequisite for the final elucidation of a criminal case. Standard methodologies, e.g. amplification of DNA followed by microsatellite typing or mitochondrial DNA sequencing, are often not sensitive enough to result in sufficient and conclusive data. We have applied ultra-deep DNA sequencing using the 454 pyrosequencing technology on a whole genome amplified (WGA) environmental biological stain, which was analysed unsuccessfully with standard methodologies following WGA. With the combination of WGA and 454 pyrosequencing, however, we were able to generate 7242 single sequences with an average length of 195 bp. A total of 1,441,971 bp DNA sequences were generated and compared with public DNA sequence databases. Using RepeatMasker and basic logical alignment search tool (BLAST) searches against known microbial and mammalian genomes it was possible to determine the metagenomic composition of the stain, i.e. 4.2% bacterial DNA, 0.3% viral DNA, 2.7% fungal DNA, 10.3% mammalian repetitive DNA, 0.9% porcine DNA, 0.13% human DNA and 81.5% DNA of unknown origin. Our data demonstrate that 454 pyrosequencing has the potential to become a powerful tool not only in basic research but also in the metagenomic analysis of biological trace materials for forensic genetics. (C) 2009 Elsevier Ireland Ltd. All rights reserved."],["dc.description.sponsorship","Erxleben Research and Innovation Council ERIC [ERIC-BR1959-2008-01]"],["dc.identifier.doi","10.1016/j.fsigen.2009.10.001"],["dc.identifier.isi","000278592500002"],["dc.identifier.pmid","20457050"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19523"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Ireland Ltd"],["dc.relation.issn","1872-4973"],["dc.title","Shotgun metagenomics of biological stains using ultra-deep DNA sequencing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","35379"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","Oncotarget"],["dc.bibliographiccitation.lastpage","35389"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Liu, Wen"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Schmidt, Laura C."],["dc.contributor.author","Roolf, Catrin"],["dc.contributor.author","Pews-Davtyan, Anahit"],["dc.contributor.author","Ruetgen, Barbara C."],["dc.contributor.author","Hammer, Sabine"],["dc.contributor.author","Willenbrock, Saskia"],["dc.contributor.author","Sekora, Anett"],["dc.contributor.author","Rolfs, Arndt"],["dc.contributor.author","Beller, Matthias"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Nolte, Ingo"],["dc.contributor.author","Junghanss, Christian"],["dc.contributor.author","Schuetz, Ekkehard"],["dc.contributor.author","Escobar, Hugo Murua"],["dc.date.accessioned","2018-11-07T10:12:52Z"],["dc.date.available","2018-11-07T10:12:52Z"],["dc.date.issued","2016"],["dc.description.abstract","Protein kinase inhibitors are widely used in chemotherapeutic cancer regimens. Maleimide derivatives such as SB-216763 act as GSK-3 inhibitor targeting cell proliferation, cell death and cell cycle progression. Herein, the two arylindolylmaleimide derivatives PDA-66 and PDA-377 were evaluated as potential chemotherapeutic agents on canine B-cell lymphoma cell lines. Canine lymphoma represents a naturally occurring model closely resembling the human high-grade non-Hodgkin's lymphoma (NHL). PDA-66 showed more pronounced effects on both cell lines. Application of 2.5 mu M PDA-66 resulted in a significant induction of apoptosis (approx. 11 %), decrease of the metabolic activity (approx. 95 %), anti-proliferative effect (approx. 85 %) and cell death within 48h. Agent induced mode of action was characterized by whole transcriptome sequencing, 12 h and 24 h post-agent exposure. Key PDA-66-modulated pathways identified were cell cycle, DNA replication and p53 signaling. Expression analyses indicated that the drug acting mechanism is mediated through DNA replication and cycle arrest involving the spindle assembly checkpoint. In conclusion, both PDA derivatives displayed strong anti-proliferation activity in canine B-cell lymphoma cells. The cell and molecular PDA-induced effect characterization and the molecular characterization of the agent acting mechanism provides the basis for further evaluation of a potential drug for canine lymphoma serving as model for human NHL."],["dc.description.sponsorship","Chinese Scholarship Council (CSC)"],["dc.identifier.doi","10.18632/oncotarget.9297"],["dc.identifier.isi","000377752100139"],["dc.identifier.pmid","27177088"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14133"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40322"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Impact Journals Llc"],["dc.relation.issn","1949-2553"],["dc.rights.access","openAccess"],["dc.title","Characterization of the novel indolylmaleimides' PDA-66 and PDA-377 effect on canine lymphoma cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","57"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","KLEINTIERPRAXIS"],["dc.bibliographiccitation.lastpage","+"],["dc.bibliographiccitation.volume","57"],["dc.contributor.author","Schutz, Ekkehard"],["dc.contributor.author","Droegemueller, Cord"],["dc.contributor.author","Leeb, Tosso"],["dc.contributor.author","Scharfenstein, Melanie"],["dc.contributor.author","Brenig, Bertram"],["dc.date.accessioned","2018-11-07T09:13:33Z"],["dc.date.available","2018-11-07T09:13:33Z"],["dc.date.issued","2012"],["dc.description.abstract","Osteogenesis imperfecta in the dachshund Osteogenesis imperfecta (OI) in the Dachshund is due to a recessive mutation in the SERPINH1 gene (serpin H1, serinarginine-protease inhibitor, heat shock protein 47, collagen-binding protein 1). An alteration or loss of function of SERPINH1 results in a misfolding of collagen fibrils and consequently to an abnormal ossification. Homozygous mutant animals are nonviable and usually die shortly after birth with multiple bone fractures often already acquired during foetal development. In the present study, we analysed 591 Dachshunds for the presence of the SERPINH1 mutation and recorded the rate of stillborn puppies. The association between both parameters was recorded in male dogs with at least 15 registered litters. The allele frequency of the SERPINH1 mutation was calculated to be 8.86%. In litters of heterozygous male Dachshunds, the mortality rate was exceedingly significantly higher (p = 0.00002; n = 3299 puppies) than in dogs without this mutation (odds-ratio: 1.8). One affected homozygous Dachshund showed the typical clinic signs of OI with brittle bones, fractures, and translucent reddish teeth. In summary, we were able show that the causative OI mutation within the SERPINH1 gene in the Dachshund results in a significantly increased mortality rate among the offspring of carriers. Due to the relatively high allele frequency of the mutated allele and animal welfare issues involved, dogs that are used for breeding should be tested using the robust DNA-based assay described here. With a consistent implementation of DNA-based diagnosis, it should be possible to eliminate carriers gradually with preservation of the genetic diversity in the breeding population."],["dc.identifier.isi","000302201800001"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27209"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","M H Schaper Gmbh Co Kg"],["dc.relation.issn","0023-2076"],["dc.title","Osteogenesis imperfecta in the dachshund"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0129208"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Schuetz, Ekkehard"],["dc.contributor.author","Hardt, Michael"],["dc.contributor.author","Scheuermann, Petra"],["dc.contributor.author","Freick, Markus"],["dc.date.accessioned","2018-11-07T09:55:52Z"],["dc.date.available","2018-11-07T09:55:52Z"],["dc.date.issued","2015"],["dc.description.abstract","Aristaless-like homeobox 4 (ALX4) gene is an important transcription regulator in skull and limb development. In humans and mice ALX4 mutations or loss of function result in a number of skeletal and organ malformations, including polydactyly, tibial hemimelia, omphalocele, biparietal foramina, impaired mammary epithelial morphogenesis, alopecia, coronal craniosynostosis, hypertelorism, depressed nasal bridge and ridge, bifid nasal tip, hypogonadism, and body agenesis. Here we show that a complex skeletal malformation of the hind limb in Galloway cattle together with other developmental anomalies is a recessive autosomal disorder most likely caused by a duplication of 20 bp in exon 2 of the bovine ALX4 gene. A second duplication of 34 bp in exon 4 of the same gene has no known effect, although both duplications result in a frameshift and premature stop codon leading to a truncated protein. Genotyping of 1,688 Black/Red/Belted/Riggit Galloway (GA) and 289 White Galloway (WGA) cattle showed that the duplication in exon 2 has allele frequencies of 1% in GA and 6% in WGA and the duplication in exon 4 has frequencies of 23% in GA and 38% in WGA. Both duplications were not detected in 876 randomly selected German Holstein Friesian and 86 cattle of 21 other breeds. Hence, we have identified a candidate causative mutation for tibial hemimelia syndrome in Galloway cattle and selection against this mutation can be used to eliminate the mutant allele from the breed."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2015"],["dc.identifier.doi","10.1371/journal.pone.0129208"],["dc.identifier.isi","000356329900052"],["dc.identifier.pmid","26076463"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11957"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36844"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights.access","openAccess"],["dc.title","A 20 bp Duplication in Exon 2 of the Aristaless-Like Homeobox 4 Gene (ALX4) Is the Candidate Causative Mutation for Tibial Hemimelia Syndrome in Galloway Cattle"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","173"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Theriogenology"],["dc.bibliographiccitation.lastpage","179"],["dc.bibliographiccitation.volume","79"],["dc.contributor.author","Mayer, Jennifer"],["dc.contributor.author","Soller, Jan T."],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Purwins, Vanessa"],["dc.contributor.author","Wemheuer, Wilhelm E."],["dc.contributor.author","Schuetz, Ekkehard"],["dc.contributor.author","Brenig, Bertram"],["dc.date.accessioned","2018-11-07T09:30:51Z"],["dc.date.available","2018-11-07T09:30:51Z"],["dc.date.issued","2013"],["dc.description.abstract","Early and accurate pregnancy diagnosis in dairy cattle is a prerequisite for successful herd management. However, most of the currently available methods allow an early diagnosis only approximately 30 days after insemination. Recently, circulating nucleic acids (CNAs) have been used successfully as biomarkers in prenatal diagnosis at different gestational stages in human and animals. Here we show that CNAs can also be used as markers for the detection of early pregnancy in cattle. Serum samples were collected from multiparous pregnant (N = 24) and nonpregnant (N = 16) dairy cows at different days after insemination (Days 0, 20, and 40). Isolated serum DNA was preprocessed using a modified serial analysis of gene expression technique, which generated concatemerized short sequence tags for downstream next generation sequencing. Bioinformatic analysis identified sequence tags specific for pregnant dairy cows at Day 20 after insemination. The identified CNA-tags originated from repetitive regions of the bovine genome. Tag sequences that showed increased hit counts per animal were used to design quantitative real-time polymerase chain reaction assays. These quantitative polymerase chain reaction assays were applied to CNA samples from matched pregnant (N = 12) and nonpregnant cows (N = 16) at different times after insemination (Day 0, 20, and 40). At Day 20 after insemination the quantities of the interspersed repeats Art2A and BovB were increased in the pregnant cows compared with the nonpregnant control cows (P < 0.05). The best performing CNA biomarker BovB yielded an area under the receiver operating characteristic curve of 0.76. At a defined cutoff value, the pregnant and the control groups can be distinguished with a sensitivity of 83% and specificity of 75%. These results suggest that CNAs can be used as biomarkers for the detection of early pregnancy in cattle. (C) 2013 Elsevier Inc. All rights reserved."],["dc.description.sponsorship","European Regional Development Fund (ERDF) [W2-80025700]"],["dc.identifier.doi","10.1016/j.theriogenology.2012.09.024"],["dc.identifier.isi","000312607400022"],["dc.identifier.pmid","23122603"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31408"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","0093-691X"],["dc.title","Early pregnancy diagnosis in dairy cows using circulating nucleic acids"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","58"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Veterinary Quarterly"],["dc.bibliographiccitation.lastpage","67"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","Zhang, Xuying"],["dc.contributor.author","Hirschfeld, Marc"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Kupke, Alexandra"],["dc.contributor.author","Köhler, Kernt"],["dc.contributor.author","Schütz, Ekkehard"],["dc.contributor.author","Brenig, Bertram"],["dc.date.accessioned","2020-12-10T18:14:48Z"],["dc.date.available","2020-12-10T18:14:48Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1080/01652176.2020.1721611"],["dc.identifier.eissn","1875-5941"],["dc.identifier.issn","0165-2176"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74619"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Osteogenesis imperfecta in a male holstein calf associated with a possible oligogenic origin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Prion"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Sensen, Maria"],["dc.contributor.author","Gordon, Paul M. K."],["dc.contributor.author","Schuetz, Ekkehard"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Urnovitz, Howard B."],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Groschup, Martin H."],["dc.contributor.author","Sutton, Ted"],["dc.contributor.author","Church, Robert B."],["dc.contributor.author","Sensen, Christoph W."],["dc.date.accessioned","2018-11-07T08:42:00Z"],["dc.date.available","2018-11-07T08:42:00Z"],["dc.date.issued","2010"],["dc.format.extent","221"],["dc.identifier.isi","000285872300276"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19600"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Landes Bioscience"],["dc.publisher.place","Austin"],["dc.relation.issn","1933-6896"],["dc.title","Identification of DNA Patterns from Circulating Nucleic Acids related to Bovine Spongiform Encephalopathy (BSE)"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]