Schütz, Ekkehard

[["dc.bibliographiccitation.journal","Haematologica"],["dc.bibliographiccitation.volume","100"],["dc.contributor.author","Oellerich, Thomas"],["dc.contributor.author","Corso, Jasmin"],["dc.contributor.author","Beck, J."],["dc.contributor.author","Pan, K.-T."],["dc.contributor.author","Döbele, Carmen"],["dc.contributor.author","Wachter, Astrid"],["dc.contributor.author","Lenz, Christof"],["dc.contributor.author","Beißbarth, Tim"],["dc.contributor.author","Schuetz, Eckehardt"],["dc.contributor.author","Tomska, Katarzyna"],["dc.contributor.author","Sellner, L."],["dc.contributor.author","Zenz, Thorsten"],["dc.contributor.author","Serve, Hubert"],["dc.contributor.author","Urlaub, Henning"],["dc.date.accessioned","2018-11-07T09:56:10Z"],["dc.date.available","2018-11-07T09:56:10Z"],["dc.date.issued","2015"],["dc.format.extent","178"],["dc.identifier.isi","000361204901386"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36907"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Ferrata Storti Foundation"],["dc.publisher.place","Pavia"],["dc.relation.eventlocation","Vienna, AUSTRIA"],["dc.relation.issn","0390-6078"],["dc.title","THE B CELL RECEPTOR SIGNALING OUTPUT IN BURKITT'S LYMPHOMA IS GENOTYPE-SPECIFIC AND IMPACTS SENSITIVITY TOWARDS BCR SIGNALING INHIBITORS"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","20"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Therapeutic Drug Monitoring"],["dc.bibliographiccitation.lastpage","26"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Oellerich, M."],["dc.contributor.author","Shipkova, Maria"],["dc.contributor.author","Schutz, Ekkehard"],["dc.contributor.author","Wieland, Eberhard"],["dc.contributor.author","Weber, Lutz T."],["dc.contributor.author","Tonshoff, B."],["dc.contributor.author","Armstrong, Victor William"],["dc.date.accessioned","2018-11-07T10:55:24Z"],["dc.date.available","2018-11-07T10:55:24Z"],["dc.date.issued","2000"],["dc.description.abstract","The need for mycophenolic acid (MPA) monitoring is still under discussion. Key issues for the PK/PD relationships of this drug are: the role of metabolites, the usefulness of AUC versus predose levels, and the need to monitor the free concentration of MPA (f-MPA). Recent advances have revealed that, in addition to 7-O-MPAG, three additional MPA metabolites are present in the plasma of transplant recipients. One of these metabolites (M-2), identified as an acyl glucuronide of MPA, was found to inhibit IMPDH-II in vitro. This active metabolite was also found to cross-react in the Emit assay for MPA. In an ongoing multicenter study, the authors are evaluating the relevance of monitoring total (t-MPA) and free mycophenolic acid (f-MPA) in pediatric renal transplant recipients. As in adults, a time-dependant increase of t-MPA-AUC(0-12h) within the first 3 months posttransplant (35 versus 64 mg x L/h, 3 weeks versus 3 months respectively; daily dosage: 0.6 g/m(2) bid) was seen. Receiver operating characteristics curve analyses were used to test the ability of predose levels or AUC(0-12h) to discriminate between cases with no complications and those with acute rejection, adverse events (severe infections, leukopenia), or gastrointestinal disorders observed during the early posttransplant course, In agreement with observations in adults, a significant (p = 0.001) association was observed between AUC(0-12h) and acute rejection. A t-MPA-AUC(0-12h) of approximately 30-60 mg x L/h, as determined by HPLC, seems to be a reasonable target for the early posttransplant period. It remains to be elucidated whether regular predose level monitoring may be of more practical value. A higher incidence of rejection was observed at predose MPA concentrations less than or equal to 1 mg/L, as measured by HPLC. In contrast to t-MPA, f-MPA-AUC(0-12h) was significantly related to seven infections and leukopenia. The risk for severe adverse events was increased at f-MPA- AUC(0-12h) values greater than or equal to 600 mu g x Uh. On the basis of these data and the observed variability in the pharmacokinetics of MPA, the development of monitoring strategies for this drug appears to he promising."],["dc.identifier.doi","10.1097/00007691-200002000-00004"],["dc.identifier.isi","000085149700004"],["dc.identifier.pmid","10688252"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49780"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","0163-4356"],["dc.title","Pharmacokinetic and metabolic investigations of mycophenolic acid in pediatric patients after renal transplantation: Implications for therapeutic drug monitoring"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","477"],["dc.bibliographiccitation.journal","Annals of the New York Academy of Sciences"],["dc.bibliographiccitation.lastpage","491"],["dc.bibliographiccitation.volume","1069"],["dc.contributor.author","Schedel, Jörg"],["dc.contributor.author","Gödde, Andrea"],["dc.contributor.author","Schütz, Ekkehard"],["dc.contributor.author","Bongartz, Tim A."],["dc.contributor.author","Lang, Bernhard"],["dc.contributor.author","Schölmerich, Jürgen"],["dc.contributor.author","Müller-Ladner, Ulf"],["dc.date.accessioned","2018-11-07T10:30:44Z"],["dc.date.available","2018-11-07T10:30:44Z"],["dc.date.issued","2006"],["dc.description.abstract","As azathioprine is one of the standard immunosuppressive drugs used for treatment of patients with different chronic inflammatory diseases, the effect of the azathioprine metabolizing enzyme thiopurine methyltransferase (TPMT) activity on incidence of adverse events (AE) was examined. In addition, potential correlations between the concentration of the azathioprine metabolite 6-thioguanine nucleotide (6-TGN) in erythrocytes (RBC) and inflammatory disease activity as well as hematological AE were investigated. TPMT activities were investigated prospectively in 139 patients (35 mate, 104 female) with chronic inflammatory diseases [systemic lupus erythematosus (SLE, 38), progressive systemic sclerosis (PSS, 13), Wegener's granulomatosis (4), rheumatoid arthritis (RA, 5), and other chronic inflammatory diseases (79)]. In addition, 6-TGN concentrations were investigated in a second cohort of 58 patients (17 patients with SLE, 5 with PSS, 5 with vasculitides, 4 with undifferentiated connective tissue diseases, 1 with dermatomyositis, 1 with Sjogren's syndrome, 1 with RA, 20 with Crohn's disease, and 4 with ulcerative colitis) prior to and during therapy with azathioprine. The distribution of activities of TPMT in 139 patients showed a normal Gaussian distribution in the Caucasian population. Within the group of 96 patients taking azathioprine, known azathioprine-related AE could be observed: minor AE (sickness, rash, and increase in cholestasis parameters) in 11 patients (11.4%), and severe AE (bone marrow toxicity) in 7 patients (7.3%). Below a \"cutoff\" value of 11.9 nmol/mL REC x h of TPMT activity, AE were significantly more frequent. In the second cohort of patients, no significant correlations could be observed between 6-TGN concentrations and parameters of disease activity. Reduced activity of TPMT in patients with chronic inflammatory diseases requiring immunosuppressive therapy with azathioprine, especially below a distinct cutoff, appears to inherit a substantial risk for development of AE."],["dc.identifier.doi","10.1196/annals.1351.048"],["dc.identifier.isi","000239951000047"],["dc.identifier.pmid","16855176"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43936"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing"],["dc.publisher.place","Oxford"],["dc.relation.conference","3rd International Conference on Neuroendocrine Immune Basis of the Rheumatic Diseases"],["dc.relation.eventend","2005-09-12"],["dc.relation.eventlocation","Genova, ITALY"],["dc.relation.eventstart","2005-09-10"],["dc.relation.isbn","1-57331-593-1"],["dc.relation.issn","0077-8923"],["dc.title","Impact of thiopurine methyltransferase activity and 6-thioguanine nucleotide concentrations in patients with chronic inflammatory diseases"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","156"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Clinical Chemistry"],["dc.bibliographiccitation.lastpage","161"],["dc.bibliographiccitation.volume","46"],["dc.contributor.author","von Ahsen, Nicolas"],["dc.contributor.author","Oellerich, M."],["dc.contributor.author","Schutz, Ekkehard"],["dc.date.accessioned","2018-11-07T10:16:50Z"],["dc.date.available","2018-11-07T10:16:50Z"],["dc.date.issued","2000"],["dc.description.abstract","Background: alpha(1)-Antitrypsin is the major plasma serine protease inhibitor. Its deficiency is mainly associated with the alleles PI S and PI Z and can lead to obstructive lung disease in adults and to liver cirrhosis during childhood. Methods: A multiplex PCR method has been established that uses two sets of primers to amplify the gene regions covering the PI S or PI Z mutations sites. Mutation detection was performed on the LightCycler by melting curve analysis of detection probes labeled with two different fluorescent dyes, LC-Red640 and LC-Red705. Results: Unequivocal genotyping results were obtained for all investigated samples in an assay time of similar to 30 min. The color compensation procedure greatly improved the readability of the resulting diagnostic melting curves. Conclusions: To our knowledge, this is the first report of simultaneous detection of two mutations in a single tube by PCR of genomic DNA and the use of two different reporter dyes with the LightCycler color compensation feature. This approach is a rapid, convenient, and economic alternative to other methods described to date for the detection of alpha(1)-antitrypsin deficiency alleles. (C) 2000 American Association for Clinical Chemistry."],["dc.identifier.isi","000085288500004"],["dc.identifier.pmid","10657370"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41113"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Assoc Clinical Chemistry"],["dc.publisher.place","Washington"],["dc.relation.conference","Meeting of the Deutsche-Gesellschaft-fur-Klinische-Chemie / Deutsche-Gesellschaft-fur-Laboratoriumsmedizin"],["dc.relation.eventlocation","REGENSBURG, GERMANY"],["dc.relation.issn","0009-9147"],["dc.title","Use of two reporter dyes without interference in a single-tube rapid-cycle PCR: alpha(1)-antitrypsin genotyping by multiplex real-time fluorescence PCR with the LightCycler"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","30"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Neuroimmunology"],["dc.bibliographiccitation.lastpage","39"],["dc.bibliographiccitation.volume","113"],["dc.contributor.author","Schmidt, H"],["dc.contributor.author","Tlustochowska, A."],["dc.contributor.author","Stuertz, K."],["dc.contributor.author","Djukic, M."],["dc.contributor.author","Gerber, Joachim"],["dc.contributor.author","Schutz, Ekkehard"],["dc.contributor.author","Kuhnt, U."],["dc.contributor.author","Nau, R."],["dc.date.accessioned","2021-06-01T10:50:09Z"],["dc.date.available","2021-06-01T10:50:09Z"],["dc.date.issued","2001"],["dc.description.abstract","Hippocampal slices of newborn rats were exposed to either heat-inactivated Streptococcus pneumoniae R6 (hiR6) equivalent to 10(6) and 10(8) CFU/ml, lipoteichoic acid (LTA) (0.3 mug/ml and 30 mug/ml), peptidoglycans (PG) (0.3, 30, 50 and 100 mug/ml), pneumococcal DNA (pDNA) (0.3 and 30 mug/ml) or medium only (control). Cell injury was examined by Nissl staining, Annexin V and NeuN immunohistochemistry, and quantified by propidium iodide (PI) uptake and by determining neuron-specific enolase (NSE) concentration in the culture medium. Necrotic and apoptotic cell damage occurred in all treatment groups. Overall damage (Nissl and PI staining) was most prominent after hiR6 (10(8) CFU/ml), followed by LTA (30 mug/ml), pDNA (30 mug/ml), and not detectable after PG (30 mug/ml) exposure. PG (100 mug/ml) induced severe damage. Apoptotic cells were most frequent after exposure to LTA and hiR6. Damage in the neuronal cell layers (NeuN, NSE) was most severe after treatment with hiR6 (10(8) CFU/ml), followed by PG (100 mug/ml), pDNA (30 mug/ml), and LTA (30 mug/ml). (C) 2001 Elsevier Science B.V. All rights reserved."],["dc.identifier.doi","10.1016/S0165-5728(00)00402-1"],["dc.identifier.isi","000166575000004"],["dc.identifier.pmid","11137574"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86547"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0165-5728"],["dc.title","Organotypic hippocampal cultures A model of brain tissue damage in Streptococcus pneumoniae meningitis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","228"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Forensic Science International Genetics"],["dc.bibliographiccitation.lastpage","231"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Beck, J."],["dc.contributor.author","Schuetz, Eckehardt"],["dc.date.accessioned","2018-11-07T08:41:39Z"],["dc.date.available","2018-11-07T08:41:39Z"],["dc.date.issued","2010"],["dc.description.abstract","A detailed molecular analysis of blood or other biological stains at a crime scene is often hampered by the low quantity and quality of the extractable DNA. However, the determination of the origin and composition of a stain is in most cases a prerequisite for the final elucidation of a criminal case. Standard methodologies, e.g. amplification of DNA followed by microsatellite typing or mitochondrial DNA sequencing, are often not sensitive enough to result in sufficient and conclusive data. We have applied ultra-deep DNA sequencing using the 454 pyrosequencing technology on a whole genome amplified (WGA) environmental biological stain, which was analysed unsuccessfully with standard methodologies following WGA. With the combination of WGA and 454 pyrosequencing, however, we were able to generate 7242 single sequences with an average length of 195 bp. A total of 1,441,971 bp DNA sequences were generated and compared with public DNA sequence databases. Using RepeatMasker and basic logical alignment search tool (BLAST) searches against known microbial and mammalian genomes it was possible to determine the metagenomic composition of the stain, i.e. 4.2% bacterial DNA, 0.3% viral DNA, 2.7% fungal DNA, 10.3% mammalian repetitive DNA, 0.9% porcine DNA, 0.13% human DNA and 81.5% DNA of unknown origin. Our data demonstrate that 454 pyrosequencing has the potential to become a powerful tool not only in basic research but also in the metagenomic analysis of biological trace materials for forensic genetics. (C) 2009 Elsevier Ireland Ltd. All rights reserved."],["dc.description.sponsorship","Erxleben Research and Innovation Council ERIC [ERIC-BR1959-2008-01]"],["dc.identifier.doi","10.1016/j.fsigen.2009.10.001"],["dc.identifier.isi","000278592500002"],["dc.identifier.pmid","20457050"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19523"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Ireland Ltd"],["dc.relation.issn","1872-4973"],["dc.title","Shotgun metagenomics of biological stains using ultra-deep DNA sequencing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","57"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","KLEINTIERPRAXIS"],["dc.bibliographiccitation.lastpage","+"],["dc.bibliographiccitation.volume","57"],["dc.contributor.author","Schutz, Ekkehard"],["dc.contributor.author","Droegemueller, Cord"],["dc.contributor.author","Leeb, Tosso"],["dc.contributor.author","Scharfenstein, Melanie"],["dc.contributor.author","Brenig, Bertram"],["dc.date.accessioned","2018-11-07T09:13:33Z"],["dc.date.available","2018-11-07T09:13:33Z"],["dc.date.issued","2012"],["dc.description.abstract","Osteogenesis imperfecta in the dachshund Osteogenesis imperfecta (OI) in the Dachshund is due to a recessive mutation in the SERPINH1 gene (serpin H1, serinarginine-protease inhibitor, heat shock protein 47, collagen-binding protein 1). An alteration or loss of function of SERPINH1 results in a misfolding of collagen fibrils and consequently to an abnormal ossification. Homozygous mutant animals are nonviable and usually die shortly after birth with multiple bone fractures often already acquired during foetal development. In the present study, we analysed 591 Dachshunds for the presence of the SERPINH1 mutation and recorded the rate of stillborn puppies. The association between both parameters was recorded in male dogs with at least 15 registered litters. The allele frequency of the SERPINH1 mutation was calculated to be 8.86%. In litters of heterozygous male Dachshunds, the mortality rate was exceedingly significantly higher (p = 0.00002; n = 3299 puppies) than in dogs without this mutation (odds-ratio: 1.8). One affected homozygous Dachshund showed the typical clinic signs of OI with brittle bones, fractures, and translucent reddish teeth. In summary, we were able show that the causative OI mutation within the SERPINH1 gene in the Dachshund results in a significantly increased mortality rate among the offspring of carriers. Due to the relatively high allele frequency of the mutated allele and animal welfare issues involved, dogs that are used for breeding should be tested using the robust DNA-based assay described here. With a consistent implementation of DNA-based diagnosis, it should be possible to eliminate carriers gradually with preservation of the genetic diversity in the breeding population."],["dc.identifier.isi","000302201800001"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27209"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","M H Schaper Gmbh Co Kg"],["dc.relation.issn","0023-2076"],["dc.title","Osteogenesis imperfecta in the dachshund"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1240"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Genes"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Gladbach, Yvonne Saara"],["dc.contributor.author","Sklarz, Lisa-Madeleine"],["dc.contributor.author","Roolf, Catrin"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Schütz, Ekkehard"],["dc.contributor.author","Fuellen, Georg"],["dc.contributor.author","Junghanss, Christian"],["dc.contributor.author","Murua Escobar, Hugo"],["dc.contributor.author","Hamed, Mohamed"],["dc.date.accessioned","2022-09-01T09:51:11Z"],["dc.date.available","2022-09-01T09:51:11Z"],["dc.date.issued","2022"],["dc.description.abstract","Little is known about optimally applying chemotherapeutic agents in a specific temporal sequence to rapidly reduce the tumor load and to improve therapeutic efficacy. The clinical optimization of drug efficacy while reducing side effects is still restricted due to an incomplete understanding of the mode of action and related tumor relapse mechanisms on the molecular level. The molecular characterization of transcriptomic drug signatures can help to identify the affected pathways, downstream regulated genes and regulatory interactions related to tumor relapse in response to drug application. We tried to outline the dynamic regulatory reprogramming leading to tumor relapse in relapsed MLL-rearranged pro-B-cell acute lymphoblastic leukemia (B-ALL) cells in response to two first-line treatments: dexamethasone (Dexa) and cytarabine (AraC). We performed an integrative molecular analysis of whole transcriptome profiles of each treatment, specifically considering public knowledge of miRNA regulation via a network-based approach to unravel key driver genes and miRNAs that may control the relapse mechanisms accompanying each treatment. Our results gave hints to the crucial regulatory roles of genes leading to Dexa-resistance and related miRNAs linked to chemosensitivity. These genes and miRNAs should be further investigated in preclinical models to obtain more hints about relapse processes."],["dc.identifier.doi","10.3390/genes13071240"],["dc.identifier.pii","genes13071240"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113900"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.relation.eissn","2073-4425"],["dc.title","Molecular Characterization of the Response to Conventional Chemotherapeutics in Pro-B-ALL Cell Lines in Terms of Tumor Relapse"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2127"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Transplantation Proceedings"],["dc.bibliographiccitation.lastpage","2128"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Braun, F."],["dc.contributor.author","Schutz, Ekkehard"],["dc.contributor.author","Peters, B."],["dc.contributor.author","Talaulicar, R."],["dc.contributor.author","Grupp, Clemens"],["dc.contributor.author","Undre, N."],["dc.contributor.author","Schafer, A."],["dc.contributor.author","Armstrong, Victor William"],["dc.contributor.author","Oellerich, M."],["dc.contributor.author","Ringe, B."],["dc.date.accessioned","2018-11-07T09:09:13Z"],["dc.date.available","2018-11-07T09:09:13Z"],["dc.date.issued","2001"],["dc.identifier.doi","10.1016/S0041-1345(01)01970-4"],["dc.identifier.isi","000168781300016"],["dc.identifier.pmid","11377473"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26209"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.publisher.place","New york"],["dc.relation.conference","4th International Conference on New Trends in Clinical and Experimental Immunosuppression"],["dc.relation.eventlocation","GENEVA, SWITZERLAND"],["dc.relation.issn","0041-1345"],["dc.title","Pharmacokinetics of tacrolimus primary immunosuppression in kidney transplant recipients"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","173"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Theriogenology"],["dc.bibliographiccitation.lastpage","179"],["dc.bibliographiccitation.volume","79"],["dc.contributor.author","Mayer, Jennifer"],["dc.contributor.author","Soller, Jan T."],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Purwins, Vanessa"],["dc.contributor.author","Wemheuer, Wilhelm E."],["dc.contributor.author","Schuetz, Ekkehard"],["dc.contributor.author","Brenig, Bertram"],["dc.date.accessioned","2018-11-07T09:30:51Z"],["dc.date.available","2018-11-07T09:30:51Z"],["dc.date.issued","2013"],["dc.description.abstract","Early and accurate pregnancy diagnosis in dairy cattle is a prerequisite for successful herd management. However, most of the currently available methods allow an early diagnosis only approximately 30 days after insemination. Recently, circulating nucleic acids (CNAs) have been used successfully as biomarkers in prenatal diagnosis at different gestational stages in human and animals. Here we show that CNAs can also be used as markers for the detection of early pregnancy in cattle. Serum samples were collected from multiparous pregnant (N = 24) and nonpregnant (N = 16) dairy cows at different days after insemination (Days 0, 20, and 40). Isolated serum DNA was preprocessed using a modified serial analysis of gene expression technique, which generated concatemerized short sequence tags for downstream next generation sequencing. Bioinformatic analysis identified sequence tags specific for pregnant dairy cows at Day 20 after insemination. The identified CNA-tags originated from repetitive regions of the bovine genome. Tag sequences that showed increased hit counts per animal were used to design quantitative real-time polymerase chain reaction assays. These quantitative polymerase chain reaction assays were applied to CNA samples from matched pregnant (N = 12) and nonpregnant cows (N = 16) at different times after insemination (Day 0, 20, and 40). At Day 20 after insemination the quantities of the interspersed repeats Art2A and BovB were increased in the pregnant cows compared with the nonpregnant control cows (P < 0.05). The best performing CNA biomarker BovB yielded an area under the receiver operating characteristic curve of 0.76. At a defined cutoff value, the pregnant and the control groups can be distinguished with a sensitivity of 83% and specificity of 75%. These results suggest that CNAs can be used as biomarkers for the detection of early pregnancy in cattle. (C) 2013 Elsevier Inc. All rights reserved."],["dc.description.sponsorship","European Regional Development Fund (ERDF) [W2-80025700]"],["dc.identifier.doi","10.1016/j.theriogenology.2012.09.024"],["dc.identifier.isi","000312607400022"],["dc.identifier.pmid","23122603"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31408"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","0093-691X"],["dc.title","Early pregnancy diagnosis in dairy cows using circulating nucleic acids"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]