Burfeind, Peter

[["dc.bibliographiccitation.firstpage","303"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Medical Genetics"],["dc.bibliographiccitation.lastpage","307"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Blanck, C."],["dc.contributor.author","Kohlhase, Juergen"],["dc.contributor.author","Engels, P."],["dc.contributor.author","Burfeind, Peter"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Bottani, A."],["dc.contributor.author","Patel, M. S."],["dc.contributor.author","Kroes, H. Y."],["dc.contributor.author","Cobben, J. M."],["dc.date.accessioned","2018-11-07T11:15:39Z"],["dc.date.available","2018-11-07T11:15:39Z"],["dc.date.issued","2000"],["dc.identifier.doi","10.1136/jmg.37.4.303"],["dc.identifier.isi","000086453000013"],["dc.identifier.pmid","10819639"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54414"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","British Med Journal Publ Group"],["dc.relation.issn","0022-2593"],["dc.title","Three novel SALL1 mutations extend the mutational spectrum in Townes-Brocks syndrome"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1123"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","MHR Basic science of reproductive medicine"],["dc.bibliographiccitation.lastpage","1131"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Tascou, S."],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Meinhardt, Andreas"],["dc.contributor.author","Schweyer, Stefan"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Trappe, Ralf"],["dc.contributor.author","Burfeind, Peter"],["dc.date.accessioned","2018-11-07T11:22:05Z"],["dc.date.available","2018-11-07T11:22:05Z"],["dc.date.issued","2001"],["dc.description.abstract","In an attempt to determine the susceptibility of spermatocytes to malignant transformation by simian virus 40 (SV40) large tumour antigen (TAg), transgenic mice harbouring a chimeric gene composed of the SV40 TAg gene fused to the 1.4 kb promoter sequence of the human phosphoglycerate kinase 2 (PGK2) gene were generated. Northern blot analysis on RNA from different tissues indicated a specific transcription of TAg in the testis of PGK2-TAg transgenic mice. Reverse transcription-polymerase chain reaction and Western blot analysis on testes at different stages of development revealed that transcription and translation of the TAg gene starts in 12-day-old testis, which coincides with the appearance of pre-leptotene spermatocytes. Germ cells of transgenic mice showed no tendency toward transformation, but in testes of both 18- and 25-day-old transgenic mice, a significantly enhanced number of spermatocytes was found. In contrast, in 42-day-old transgenic mice no differences in the number of spermatocytes and spermatids were observed. The number of Sertoli cells was determined to be equal in transgenic and wild type mice. In-situ end labelling of fragmented DNA revealed a higher rate of apoptosis in testes of 18-day-old transgenic mice as compared with wild type mice. These results indicate that germ cell homeostasis in transgenic mice is maintained by an apoptotic mechanism."],["dc.identifier.doi","10.1093/molehr/7.12.1123"],["dc.identifier.isi","000172625800004"],["dc.identifier.pmid","11719589"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55922"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1360-9947"],["dc.title","Targeted expression of SV40 large tumour antigen (TAg) induces a transient enhancement of spermatocyte proliferation and apoptosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","788"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biology of Reproduction"],["dc.bibliographiccitation.lastpage","796"],["dc.bibliographiccitation.volume","69"],["dc.contributor.author","Mannan, Ashraf U."],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Mueller, C."],["dc.contributor.author","Burfeind, Peter"],["dc.contributor.author","Adham, Ibrahim M."],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T10:36:20Z"],["dc.date.available","2018-11-07T10:36:20Z"],["dc.date.issued","2003"],["dc.description.abstract","The testicular haploid expressed gene (Theg) encodes for a novel similar to42.0-kDa nuclear protein, which is specifically expressed in spermatid cells. Its expression is upregulated by some unknown factor(s) from Sertoli cells. To elucidate the function of Theg protein and its role in spermatogenesis, we disrupted the Theg locus in mouse by homologous recombination. For functional dissection of the domain structure of the Theg protein, two different knockout approaches were undertaken. In the first knockout mouse (Th14), the C-terminal region of the Theg protein (amino acids 137-376) was deleted. Both Th14(+/-) and Th14(-/-) mice from genetic backgrounds of C57BL/6J X 129X1/ SvJ hybrid and 129X1/SvJ inbred exhibited a normal phenotype and were fertile. The testes of Th14(-/-) mice were smaller than those of Th14(+/-) and Th14(+/+) mice; however, the testicular morphology and the properties of sperm, including morphology and motility, from Th14(-/-) mice were similar to those of Th14(+/-) and Th14(+/+) mice. These results demonstrate that the C-terminal region of Theg (amino acids 137-376) does not play an important role in progression of spermatogenesis. In the second knockout mouse (Th15), we deleted the N-terminal domain of the Theg protein, which resulted in complete loss of Theg transcripts. Both Th15(+/-) and Th15(-/-) mice from genetic backgrounds C57BL/6J x 129X1/SvJ hybrid, C3H/J congenic, and 129X1/SvJ inbred appeared normal and were fertile, with no gross abnormalities detected in testicular morphology or sperm properties. Our results from both knockout mouse model systems clearly illustrate that Theg is not essential for spermatogenesis in the mouse."],["dc.identifier.doi","10.1095/biolreprod.103.017400"],["dc.identifier.isi","000184989100007"],["dc.identifier.pmid","12748127"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45299"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Study Reproduction"],["dc.relation.issn","0006-3363"],["dc.title","Male mice lacking the theg (Testicular haploid expressed gene) protein undergo normal spermatogenesis and are fertile"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","584"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Molecular and Cellular Neuroscience"],["dc.bibliographiccitation.lastpage","601"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Bohm, D."],["dc.contributor.author","Schwegler, H."],["dc.contributor.author","Kotthaus, L."],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Rickmann, Michael"],["dc.contributor.author","Kohler, M."],["dc.contributor.author","Rosenbusch, J."],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Flugge, G."],["dc.contributor.author","Burfeind, Peter"],["dc.date.accessioned","2018-11-07T09:46:43Z"],["dc.date.available","2018-11-07T09:46:43Z"],["dc.date.issued","2002"],["dc.description.abstract","Aberrant reorganization of hippocampal mossy fibers occurs in human temporal lobe epilepsy and rodent epilepsy models. We generated a mouse model showing massive late-onset aberrant mossy fiber sprouting in the adult hippocampus. The mutation in this mouse model derives from an intronic insertion of transgene DNA in the mouse PLC-beta1 gene (PLC-beta1(TC)(-/-) mutation) leading to a splice mutation of the PLC-beta1 gene and a complete loss of downstream PLC-beta1 expression. PLC-beta1(TC)(-/-) mutants develop a loss of NMDA-receptors in the stratum oriens of region CA1, apoptotic neuronal death, and reduced hippocampal PKC activity. The phenotype of these mice further consists of a late-onset epileptiform hyperexcitability, behavioral modifications in a radial maze and in an open field, female nurturing defect, and male infertility. In the present study, we provide evidence that the arising of the behavioral phenotype in PLC-beta1(TC)(-/-) mice correlates in time with the development of the aberrant mossy fiber projections and that the disruption of the PLC-beta1-mediated signal transduction pathway may lead to a functional cholinergic denervation, which could cause hippocampal remodeling and, in consequence, epileptiform hyperexcitability."],["dc.identifier.doi","10.1006/mcne.2002.1199"],["dc.identifier.isi","000180026300006"],["dc.identifier.pmid","12504592"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34945"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","1044-7431"],["dc.title","Disruption of PLC-beta 1-mediated signal transduction in mutant mice causes age-dependent hippocampal mossy fiber sprouting and neurodegeneration"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Molecular and Cellular Endocrinology"],["dc.bibliographiccitation.lastpage","18"],["dc.bibliographiccitation.volume","200"],["dc.contributor.author","Tascou, S."],["dc.contributor.author","Trappe, Ralf"],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Jarry, Hubertus"],["dc.contributor.author","Konig, F."],["dc.contributor.author","Schulz-Schaeffer, Walter J."],["dc.contributor.author","Saeger, W."],["dc.contributor.author","Meinhardt, Andreas"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Schmidtke, Joerg"],["dc.contributor.author","Burfeind, Peter"],["dc.date.accessioned","2018-11-07T10:40:52Z"],["dc.date.available","2018-11-07T10:40:52Z"],["dc.date.issued","2003"],["dc.description.abstract","In an attempt to determine the susceptibility of spermatogonia to malignant transformation transgenic mice were generated harboring a 1.3 kb 5'-flanking region of the germ cell specific expressed human testis specific protein, Y-encoded gene fused with the simian virus 40 large T antigen (TAg). Unexpectedly, TAg expression in transgenic mice was also detected in somatic tissues. Between days 65 and 85 after birth most of the transgenic mice developed anterior lobe tumors of the pituitary gland and to a less extent medulla type tumors of the adrenal gland. In addition, a few older transgenic mice developed tumors of the seminal vesicle, but no testicular tumors were observed in transgenic mice up to an age of 5 months. The pituitary tumors were immunoreactive for anti-prolactin (PRL) and anti-adrenocorticotropic hormone (ACTH). PRL and corticosterone concentrations in serum of transgenic mice were significantly increased. Taken together, our studies provide a novel mouse model for pituitary adenomas displaying a unique combination of hormone expression by tumor cells secreting PRL and ACTH. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved."],["dc.identifier.doi","10.1016/S0303-7207(02)00426-4"],["dc.identifier.isi","000181913300002"],["dc.identifier.pmid","12644295"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/46406"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Sci Ireland Ltd"],["dc.relation.issn","0303-7207"],["dc.title","TSPY-LTA transgenic mice develop endocrine tumors of the pituitary and adrenal gland"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","155"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Cellular Biochemistry"],["dc.bibliographiccitation.lastpage","162"],["dc.bibliographiccitation.volume","83"],["dc.contributor.author","Schulten, Hans-Juergen"],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Reim, K."],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Burfeind, Peter"],["dc.date.accessioned","2018-11-07T09:34:48Z"],["dc.date.available","2018-11-07T09:34:48Z"],["dc.date.issued","2001"],["dc.description.abstract","The testis-specific proacrosin gene encodes for a fertilization-promoting protein. In mouse and rat it is first transcribed in late pachytene spermatocytes and revealed to be translationally regulated. Former proacrosin promoter studies demonstrated that elements necessary for conducting a stage and temporal-specific expression of the gene are located within 0.9 kb upstream of the translational start codon. in the present study we analyzed putative cis-acting elements located in this promoter region for their specific binding properties to nuclear factors assumed to be involved in proacrosin gene regulation. Supplement of specific antibodies in electrophoretic mobility shift assays (EMSA) revealed that two Y-box proteins and the transcription factors CREM and YY1 interact with proacrosin promoter elements. The Y-box proteins, antigenically related to the frog Y-box proteins FRGY1 and FRGY2, bound to the Y-box (55-66 bp upstream of the ATG initiation codon) in brain and testis nuclear extracts, respectively. CREM bound to three elements (30-37, 252-259, and 717-724 bp upstream of ATG). The ubiquitous transcription factor YY1 bound to a conserved element in the central proacrosin promoter (457-473 bp upstream of ATG) and showed almost germ cell-specific truncates in EMSA. These results suggest that the identified factors are involved in proacrosin gene regulation. J. Cell. Biochem. 83: 155-162, 2001. (C) 2001 Wiley-Liss, Inc."],["dc.identifier.doi","10.1002/jcb.1226"],["dc.identifier.isi","000170779800016"],["dc.identifier.pmid","11500964"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32254"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","0730-2312"],["dc.title","Assessment of promoter elements of the germ cell-specific proacrosin gene"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1555"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Biology of Reproduction"],["dc.bibliographiccitation.lastpage","1561"],["dc.bibliographiccitation.volume","63"],["dc.contributor.author","Tascou, S."],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Samani, A."],["dc.contributor.author","Schmidtke, Joerg"],["dc.contributor.author","Vogel, Tanja"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Burfeind, Peter"],["dc.date.accessioned","2018-11-07T11:09:47Z"],["dc.date.available","2018-11-07T11:09:47Z"],["dc.date.issued","2000"],["dc.description.abstract","We developed a novel promoter-based selection strategy that could be used to produce cell lines representing sequential stages of spermatogenesis, The method is based on immortalization and subsequent targeted selection by using differentiation-specific promoter regions. As an example for this approach, a new murine germ cell line (CC-4spc) was established using a vector construct that contains the SV40 large T antigen and the neomycin phosphotransferase II gene under the control of the SV40 early promoter and a spermatocyte-specific promoter for human phosphoglycerate kinase 2, respectively. The GC-4spc was characterized as a cell line at the stage between preleptotene and early pachytene spermatocytes. Transcription of three germ cell-specific expressed genes, Pgk2, proacrosin, and the A-myb proto-oncogene, were detected in the CC-4spc cell line using reverse transcription-polymerase chain reaction. Furthermore, TSPY (human testis-specific protein, Y-encoded) and PGK2 (human phosphoglycerate kinase 2) promoter regions showed different transcriptional activities in the GC-4spc cell line compared with the spermatogonia-derived cell line GC-1spg. Thus, our strategy could be used for immortalization of cells at specific stages of differentiation, allowing production of a series of cultured cell lines representing sequential stages of differentiation in given cell lineages."],["dc.identifier.doi","10.1095/biolreprod63.5.1555"],["dc.identifier.isi","000165089000043"],["dc.identifier.pmid","11058565"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53082"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Study Reproduction"],["dc.relation.issn","0006-3363"],["dc.title","Immortalization of murine male germ cells at a discrete stage of differentiation by a novel directed promoter-based selection strategy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","PII S0006-291X(02)00850-1"],["dc.bibliographiccitation.firstpage","319"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biochemical and Biophysical Research Communications"],["dc.bibliographiccitation.lastpage","327"],["dc.bibliographiccitation.volume","296"],["dc.contributor.author","Trappe, Ralf"],["dc.contributor.author","Buddenberg, P."],["dc.contributor.author","Uedelhoven, J."],["dc.contributor.author","Gläser, Brigitta"],["dc.contributor.author","Buck, Anja"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Burfeind, Peter"],["dc.date.accessioned","2018-11-07T10:10:34Z"],["dc.date.available","2018-11-07T10:10:34Z"],["dc.date.issued","2002"],["dc.description.abstract","The Znfl31/ZNFl31 protein belongs to the superfamily of POK proteins containing a BTB/POZ domain in its N-terminal part and 5 typical C2H2 zinc fingers and an additional C2H2 zinc finger structure in its C-terminal region. In mouse and human two alternatively spliced transcripts are expressed from the Znfl31/ZNFl31 gene, resulting from intraexonic splicing. While the longer transcript encodes for three double zinc finger structures the shorter transcript lacks the region coding for the first zinc finger. Although the murine Znfl31 gene is ubiquitously expressed, expression analysis applying whole mount in situ hybridization showed a predominant expression in the developing central nervous system with strongest signals in the forebrain, midbrain, and hindbrain areas and in the neural tube. Further dominant expression was seen in embryonic limb buds. In human adult tissues a predominant expression of ZNFl31 was seen in different brain areas, i,e., the occipital and temporal lobe, the nucleus caudatus, hippocampus, and the cerebellum as well as in testis and thymus. Therefore. it is possible that Znfl31/ZVFl31 plays a role during development and organogenesis as well as in the function of the adult central nervous system. (C) 2002 Elsevier Science (USA). All rights reserved."],["dc.identifier.doi","10.1016/S0006-291X(02)00850-1"],["dc.identifier.isi","000177525800015"],["dc.identifier.pmid","12163020"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39882"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","0006-291X"],["dc.title","The murine BTB/POZ zinc finger gene Znf131: predominant expression in the developing central nervous system, in adult brain, testis, and thymus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","171"],["dc.bibliographiccitation.issue","1-4"],["dc.bibliographiccitation.journal","CYTOGENETICS AND CELL GENETICS"],["dc.bibliographiccitation.lastpage","179"],["dc.bibliographiccitation.volume","91"],["dc.contributor.author","Mannan, Ashraf U."],["dc.contributor.author","Lucke, K."],["dc.contributor.author","Dixkens, C."],["dc.contributor.author","Neesen, J."],["dc.contributor.author","Kamper, M."],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Burfeind, Peter"],["dc.date.accessioned","2018-11-07T11:03:03Z"],["dc.date.available","2018-11-07T11:03:03Z"],["dc.date.issued","2000"],["dc.description.abstract","We have previously isolated and characterized the mouse Testicular Haploid Expressed Gene (Theg) that is specifically expressed in haploid germ cells. We now describe the molecular cloning and characterization of the human homologue (THEG) of mouse Theg. Expression studies by using both dot blot and Northern blot techniques revealed that human THEG is expressed specifically in the testis. Additionally, we found two alternatively spliced transcripts (THEG major and THEG minor) for THEG by using reverse transcription-polymerase chain reaction on human testicular RNA. Sequence analysis of these PCR products demonstrated that the smaller transcript (THEG minor) lacks 72 bp which was also observed for the mouse Theg. We have isolated the cDNAs of human THEG major and THEG minor, containing the complete open reading frames, which encode putative nuclear proteins of 379 amino acids and 355 amino acids, respectively. Database searches identified two genomic clones on chromosome 19 harboring the human THEG gene, which is approximately 14 kb pairs in size, contains eight exons, and comparison of the two cDNA sequences with the genomic sequence indicated that the smaller transcript lacks exon 3. Furthermore, we assigned the human THEG gene (THEG) to human chromosome 19ptel-->p13 by fluorescence in situ hybridization. Moreover, we detected mouse THEG protein prominently in the nucleus of round spermatids by using an antibody against THEG on both testicular sections and cellular suspensions. Additionally, the subcellular localization of mouse THEG was confirmed by a green fluorescent protein (GFP) fusion protein of mouse THEG which was found mainly in the nucleus of transfected NIH3T3 cells. These data suggest that both human and mouse THEG are specifically expressed in the nucleus of haploid male germ cells and are involved in the regulation of nuclear functions. Copyright (C),2001 S.Karger AG, Basel."],["dc.identifier.doi","10.1159/000056840"],["dc.identifier.isi","000166856000034"],["dc.identifier.pmid","11173852"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51528"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.relation.issn","0301-0171"],["dc.title","Alternative splicing, chromosome assignment and subcellular localization of the testicular haploid expressed gene (THEG)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","567"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","International Journal of Oncology"],["dc.bibliographiccitation.lastpage","574"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Tascou, S."],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Uedelhoven, J."],["dc.contributor.author","Bohm, D."],["dc.contributor.author","Jalal, R."],["dc.contributor.author","Ahmed, M."],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Burfeind, Peter"],["dc.date.accessioned","2018-11-07T09:19:52Z"],["dc.date.available","2018-11-07T09:19:52Z"],["dc.date.issued","2001"],["dc.description.abstract","In an attempt to elucidate the potential of premeiotic male germ cells to malignant transformation both the invasiveness and the differential gene expression of several putative tumor markers of the spermatogonia-derived cell line GC-1spg and the spermatocyte-derived ceLl line GC-4spc were analyzed. Studies, using RT-PCR analysis, of the expression pattern of the alkaline phosphatase isoenzymes which serve as markers for testicular germ cell tumors demonstrated that the expression of the endogenous mouse embryonic alkaline phosphatase (EAP) is upregulated in the GC-1spg cell line. Additionally, after transfection of GC-1spg cells and GC-4spc cells with a GCAP-CAT construct, an increased promoter activity of the human germ cell alkaline phosphatase (GCAP), the equivalent human isoenzyme of EAP, was shown in GC-1spg. Furthermore, an in vitro Matrigel invasion assay revealed a significant higher invasive potential of GC-1spg cells as compared to GC-4spc cells. Finally, a suppression subtractive hybridization on RNA of invasive GC-1spg cells and non-invasive GC-4spc cells was performed. In total, 31 cDNA sequences were isolated and further analyzed. Among these, 18 known sequences and 13 unknown sequences were determined. Northern blot analysis revealed that one unknown gene and eight known genes, namely integrin alpha6, L6 antigen, annexin VIII, BVL-1 retrotransposon, protective protein, replacement variant histone 3.3, alpha -catenin and LPS-binding protein, are over-expressed in invasive GC-1spg cells. Taken together, both the enhanced invasive activity of GC-1spg cells and the upregulated expression of genes involved in the process of tumor progression suggest that the immortalized spermatogonia-derived cell line GC-1spg does have a higher potential to malignant transformation than the immortalized spermatocyte-derived cell line GC-4spc."],["dc.identifier.isi","000167016800018"],["dc.identifier.pmid","11179488"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28742"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Professor D A Spandidos"],["dc.relation.issn","1019-6439"],["dc.title","Isolation and characterization of differentially expressed genes in invasive and non-invasive immortalized murine male germ cells in vitro"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]