Hummel, Susanne

[["dc.bibliographiccitation.artnumber","7"],["dc.bibliographiccitation.journal","Mobile DNA"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Kothe, Maximilian"],["dc.contributor.author","Seidenberg, Verena"],["dc.contributor.author","Hummel, Susanne"],["dc.contributor.author","Piskurek, Oliver"],["dc.date.accessioned","2018-11-07T10:15:29Z"],["dc.date.available","2018-11-07T10:15:29Z"],["dc.date.issued","2016"],["dc.description.abstract","Background: As Short Interspersed Elements (SINEs), human-specific Alu elements can be used for population genetic studies. Very recent inserts are polymorphic within and between human populations. In a sample of 30 elements originating from three different Alu subfamilies, we investigated whether they are preserved in prehistorical skeletal human remains from the Bronze Age Lichtenstein cave in Lower Saxony, Germany. In the present study, we examined a prehistoric triad of father, mother and daughter. Results: For 26 of the 30 Alu loci investigated, definite results were obtained. We were able to demonstrate that presence/absence analyses of Alu elements can be conducted on individuals who lived 3,000 years ago. The preservation of the ancient DNA (aDNA) is good enough in two out of three ancient individuals to routinely allow the amplification of 500 bp fragments. The third individual revealed less well-preserved DNA, which results in allelic dropout or complete amplification failures. We here present an alternative molecular approach to deal with these degradation phenomena by using internal Alu subfamily specific primers producing short fragments of approximately 150 bp. Conclusions: Our data clearly show the possibility of presence/absence analyses of Alu elements in individuals from the Lichtenstein cave. Thus, we demonstrate that our method is reliably applicable for aDNA samples with good or moderate DNA preservation. This method will be very useful for further investigations with more Alu loci and larger datasets. Human population genetic studies and other large-scale investigations would provide insight into Alu SINE-based microevolutionary processes in humans during the last few thousand years and help us comprehend the evolutionary dynamics of our genome."],["dc.identifier.doi","10.1186/s13100-016-0063-y"],["dc.identifier.isi","000374742800001"],["dc.identifier.pmid","27096009"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13219"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40820"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1759-8753"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Alu SINE analyses of 3,000-year-old human skeletal remains: a pilot study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","48"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Diversity"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Haß, Fabian"],["dc.contributor.author","Hummel, Susanne"],["dc.contributor.author","Piskurek, Oliver"],["dc.date.accessioned","2019-07-09T11:44:37Z"],["dc.date.available","2019-07-09T11:44:37Z"],["dc.date.issued","2017"],["dc.description.abstract","The human-specific Alu elements, belonging to the class of Short INterspersed Elements (SINEs), have been shown to be a powerful tool for population genetic studies. An earlier study in this department showed that it was possible to analyze Alu presence/absence in 3000-year-old skeletal human remains from the Bronze Age Lichtenstein cave in Lower Saxony, Germany. We developed duplex Alu screening PCRs with flanking primers for two Alu elements, each combined with a single internal Alu primer. By adding an internal primer, the approximately 400–500 bp presence signals of Alu elements can be detected within a range of less than 200 bp. Thus, our PCR approach is suited for highly fragmented ancient DNA samples, whereas NGS analyses frequently are unable to handle repetitive elements. With this analysis system, we examined remains of 12 individuals from the Lichtenstein cave with different degrees of DNA degradation. The duplex PCRs showed fully informative amplification results for all of the chosen Alu loci in eight of the 12 samples. Our analysis system showed that Alu presence/absence analysis is possible in samples with different degrees of DNA degradation and it reduces the amount of valuable skeletal material needed by a factor of four, as compared with a singleplex approach."],["dc.identifier.doi","10.3390/d9040048"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14840"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59049"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","1424-2818"],["dc.relation.issn","1573-501X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Duplex Alu Screening for Degraded DNA of Skeletal Human Remains"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]