Hummel, Susanne

[["dc.bibliographiccitation.firstpage","137"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Entomological Science"],["dc.bibliographiccitation.lastpage","141"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Buesse, Sebastian"],["dc.contributor.author","von Grumbkow, Philipp"],["dc.contributor.author","Mazanec, Janine"],["dc.contributor.author","Troester, Gert"],["dc.contributor.author","Hummel, Susanne"],["dc.contributor.author","Hoernschemeyer, Thomas"],["dc.date.accessioned","2018-11-07T10:28:52Z"],["dc.date.available","2018-11-07T10:28:52Z"],["dc.date.issued","2017"],["dc.description.abstract","A protocol using insect specimens or parts thereof allows for sequencing of sections of nuclear 28S rDNA. In the present note it is demonstrated that this protocol can readily be applied to strongly degraded DNA (ancient, fixed or contaminated). Primers that are specifically designed to discriminate against humanDNAbut also other non-arthropod species are tested on a range of species covering all insect groups (59 insect species from all 33 orders). Additionally, the samples represent a selection of various, mostly DNA-degrading, preservation methods, including the most common fixatives used for morphological investigations and for long-term storage in collections. Successful amplification was possible for all tested samples including ca. 200 year-old dried museum specimens as well as for over 4000 year-old fossil insects embedded in copal. When the NCBI database contained information on the tested species an unambiguous taxonomic discrimination was possible. This approach is based on a standardized protocol that guarantees easy application. This note presents primer pairs for 28S rDNA that can be a useful tool for ancient DNA (aDNA) research."],["dc.identifier.doi","10.1111/ens.12242"],["dc.identifier.isi","000396406800017"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43520"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Wiley"],["dc.relation.issn","1479-8298"],["dc.relation.issn","1343-8786"],["dc.title","Note on using nuclear 28S rDNA for sequencing ancient and strongly degraded insect DNA"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","43"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Diversity"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Flux, Anna Lena"],["dc.contributor.author","Mazanec, Janine"],["dc.contributor.author","Strommenger, Birgit"],["dc.contributor.author","Hummel, Susanne"],["dc.date.accessioned","2019-07-09T11:44:36Z"],["dc.date.available","2019-07-09T11:44:36Z"],["dc.date.issued","2017"],["dc.description.abstract","Staphylococcus aureus is a major pathogen causing osteomyelitis, amongst other diseases, and its methicillin-resistant form (MRSA) in particular poses a huge threat to public health. To increase our knowledge of the origin and evolution of S. aureus, genetic studies of historical microorganisms may be beneficial. Thus, the aim of this study was to investigate whether osteomyelitic skeletal material (autopsy specimens collected from the mid 19th century until the 1920s) is suitable for detecting historical S. aureus DNA sequences. We established a PCR-based analysis system targeting two specific genes of S. aureus (nuc and fib). We successfully amplified the historical S. aureus nuc and fib sequences for six and seven pre-antibiotic, osteomyelitic bone specimens, respectively. These results encourage further investigations of historical S. aureus genomes that may increase our understanding of pathogen evolution in relation to anthropogenically introduced antibiotics."],["dc.identifier.doi","10.3390/d9040043"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14836"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59047"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1424-2818"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Staphylococcus aureus Sequences from Osteomyelitic Specimens of a Pathological Bone Collection from Pre-Antibiotic Times"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3768"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Journal of Archaeological Science"],["dc.bibliographiccitation.lastpage","3776"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","v Grumbkow, Philipp"],["dc.contributor.author","Frommer, Soeren"],["dc.contributor.author","Kootker, Lisette M."],["dc.contributor.author","Davies, Gareth R."],["dc.contributor.author","Mazanec, Janine"],["dc.contributor.author","Hummel, Susanne"],["dc.date.accessioned","2018-11-07T09:19:41Z"],["dc.date.available","2018-11-07T09:19:41Z"],["dc.date.issued","2013"],["dc.description.abstract","Excavation in and around a Medieval church in Gammertingen, Germany, revealed the skeletal remains of eight individuals dating to the 10th and 11th century AD. Archaeologists hypothesized that the individuals were the first members of a family later known as the Counts of Gammertingen, a medieval high nobility family. In an interdisciplinary approach, Strontium isotope and ancient DNA techniques were performed in order to test the hypothesis that the church was used as a family burial site and to investigate the provenance of family members. Seven of the eight individuals can be placed in a three-generation genealogy. The isotope analyses establish that the eighth individual had a different birthplace and possibly became a member of the Counts of Gammertingen through marriage. Further, genetic data revealed that distant relatives of the paternal lineage are still present in this area today. Thus, the combined results lead to a very detailed knowledge about a 1000-year old noble family. (C) 2013 Elsevier Ltd. All rights reserved."],["dc.description.sponsorship","City of Gammertingen"],["dc.identifier.doi","10.1016/j.jas.2013.05.001"],["dc.identifier.isi","000323298500020"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28696"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Ltd- Elsevier Science Ltd"],["dc.relation.issn","0305-4403"],["dc.title","Kinship and mobility in 11th-century A.D. Gammertingen, Germany: an interdisciplinary approach"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","376"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BioTechniques"],["dc.bibliographiccitation.lastpage","381"],["dc.bibliographiccitation.volume","71"],["dc.contributor.author","Euskirchen, Alina"],["dc.contributor.author","Hartmann, Larissa"],["dc.contributor.author","Mazanec, Janine"],["dc.contributor.author","Wittmeier, Patrick"],["dc.contributor.author","Hummel, Susanne"],["dc.date.accessioned","2021-08-12T07:45:41Z"],["dc.date.available","2021-08-12T07:45:41Z"],["dc.date.issued","2021"],["dc.description.abstract","DNA extraction is of utmost importance in archaeobiology, as it determines the success of further DNA analyses. This study concentrates on the success of ancient DNA extraction using silica spin columns and PCR-based analysis from archaeological skeletal material and investigates the influence of sample quantity, lysis time and lysis temperature during sample preparation. The results show that lysis times ranging from 2 to 48 h are suitable, and that lysis should be carried out at a constant temperature of 56°C. Concerning sample quantity, 10 mg for mitochondrial DNA and 50 mg for chromosomal DNA are sufficient for high quality analyses. Thus invaluable sample material can be saved, and time of sample preparation can be reduced considerably."],["dc.description.abstract","METHOD SUMMARY An optimized protocol for sample preparation for the extraction of ancient DNA from archaeological skeletal material is presented. The tested and optimized parameters were sample quantity, lysis duration and lysis temperature."],["dc.description.abstract","DNA extraction is of utmost importance in archaeobiology, as it determines the success of further DNA analyses. This study concentrates on the success of ancient DNA extraction using silica spin columns and PCR-based analysis from archaeological skeletal material and investigates the influence of sample quantity, lysis time and lysis temperature during sample preparation. The results show that lysis times ranging from 2 to 48 h are suitable, and that lysis should be carried out at a constant temperature of 56°C. Concerning sample quantity, 10 mg for mitochondrial DNA and 50 mg for chromosomal DNA are sufficient for high quality analyses. Thus invaluable sample material can be saved, and time of sample preparation can be reduced considerably."],["dc.description.abstract","METHOD SUMMARY An optimized protocol for sample preparation for the extraction of ancient DNA from archaeological skeletal material is presented. The tested and optimized parameters were sample quantity, lysis duration and lysis temperature."],["dc.identifier.doi","10.2144/btn-2020-0169"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88525"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation.eissn","1940-9818"],["dc.relation.issn","0736-6205"],["dc.title","The influence of sample quantity and lysis parameters on the success of ancient DNA extraction from skeletal remains"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]