Hummel, Susanne

[["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","BioTechniques"],["dc.bibliographiccitation.volume","72"],["dc.contributor.author","Wittmeier, Patrick"],["dc.contributor.author","Hummel, Susanne"],["dc.date.accessioned","2022-04-01T10:01:30Z"],["dc.date.available","2022-04-01T10:01:30Z"],["dc.date.issued","2022"],["dc.description.abstract","Agarose gel electrophoresis is a relatively easy to use method, commonly applied to evaluate PCR reaction success. Intercalating agents or dyes are used to visualize the amplified fragments. However, it is uncertain to what extent the brightness of bands is informative about the concentration of the amplicons. To more closely examine the suitability of agarose gel electrophoresis to assess PCR product yield, we quantified the brightness of bands on a gel and compared these data with the results from spectrophotometry, fluorometry and qPCR. Evaluation of the results suggests that assessment of the relative quantity of amplicons by band brightness is precise enough even for post-PCR analysis steps requiring PCR product concentrations within a certain range to function properly."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.2144/btn-2021-0094"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/105680"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation.eissn","1940-9818"],["dc.relation.issn","0736-6205"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","7"],["dc.bibliographiccitation.journal","Mobile DNA"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Kothe, Maximilian"],["dc.contributor.author","Seidenberg, Verena"],["dc.contributor.author","Hummel, Susanne"],["dc.contributor.author","Piskurek, Oliver"],["dc.date.accessioned","2018-11-07T10:15:29Z"],["dc.date.available","2018-11-07T10:15:29Z"],["dc.date.issued","2016"],["dc.description.abstract","Background: As Short Interspersed Elements (SINEs), human-specific Alu elements can be used for population genetic studies. Very recent inserts are polymorphic within and between human populations. In a sample of 30 elements originating from three different Alu subfamilies, we investigated whether they are preserved in prehistorical skeletal human remains from the Bronze Age Lichtenstein cave in Lower Saxony, Germany. In the present study, we examined a prehistoric triad of father, mother and daughter. Results: For 26 of the 30 Alu loci investigated, definite results were obtained. We were able to demonstrate that presence/absence analyses of Alu elements can be conducted on individuals who lived 3,000 years ago. The preservation of the ancient DNA (aDNA) is good enough in two out of three ancient individuals to routinely allow the amplification of 500 bp fragments. The third individual revealed less well-preserved DNA, which results in allelic dropout or complete amplification failures. We here present an alternative molecular approach to deal with these degradation phenomena by using internal Alu subfamily specific primers producing short fragments of approximately 150 bp. Conclusions: Our data clearly show the possibility of presence/absence analyses of Alu elements in individuals from the Lichtenstein cave. Thus, we demonstrate that our method is reliably applicable for aDNA samples with good or moderate DNA preservation. This method will be very useful for further investigations with more Alu loci and larger datasets. Human population genetic studies and other large-scale investigations would provide insight into Alu SINE-based microevolutionary processes in humans during the last few thousand years and help us comprehend the evolutionary dynamics of our genome."],["dc.identifier.doi","10.1186/s13100-016-0063-y"],["dc.identifier.isi","000374742800001"],["dc.identifier.pmid","27096009"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13219"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40820"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1759-8753"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Alu SINE analyses of 3,000-year-old human skeletal remains: a pilot study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","99"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","American Journal of Physical Anthropology"],["dc.bibliographiccitation.lastpage","109"],["dc.bibliographiccitation.volume","172"],["dc.contributor.author","Schmidt, Nicole"],["dc.contributor.author","Schücker, Katharina"],["dc.contributor.author","Krause, Ina"],["dc.contributor.author","Dörk, Thilo"],["dc.contributor.author","Klintschar, Michael"],["dc.contributor.author","Hummel, Susanne"],["dc.date.accessioned","2021-04-14T08:27:44Z"],["dc.date.available","2021-04-14T08:27:44Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract Objective A genome‐wide high‐throughput single nucleotide polymorphism (SNP) typing method was tested with respect of the applicability to ancient and degraded DNA. The results were compared to mini‐sequencing data achieved through single base extension (SBE) typing. The SNPs chosen for the study allow to determine the hair colors and eye colors of humans. Material and methods The DNA samples were extracted from the skeletal remains of 59 human individuals dating back to the Late Bronze Age. The 3,000 years old bones had been discovered in the Lichtenstein Cave in Lower Saxony, Germany. The simultaneous typing of 24 SNPs for each of the ancient DNA samples was carried out using the 192.24 Dynamic Array™ by Fluidigm®. Results Thirty‐eight of the ancient samples (=64%) revealed full and reproducible SNP genotypes allowing hair and eye color phenotyping. In 10 samples (=17%) at least half of the SNPs were unambiguously determined, in 11 samples (=19%) the SNP typing failed. For 23 of the 59 individuals, a comparison of the SNP typing results with genotypes from an earlier performed SBE typing approach was possible. The comparison confirmed the full concordance of the results for 90% of the SNP typings. In the remaining 10% allelic dropouts were identified. Discussion The high genotyping success rate could be achieved by introducing modifications to the preamplification protocol mainly by increasing the DNA input and the amplification cycle number. The occurrence of allelic dropouts indicates that a further increase of DNA input to the preamplification step is desirable."],["dc.identifier.doi","10.1002/ajpa.23996"],["dc.identifier.eissn","1096-8644"],["dc.identifier.issn","0002-9483"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82386"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","John Wiley \\u0026 Sons, Inc."],["dc.relation.eissn","1096-8644"],["dc.relation.issn","0002-9483"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],["dc.title","Genome‐wide SNP typing of ancient DNA: Determination of hair and eye color of Bronze Age humans from their skeletal remains"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","43"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Diversity"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Flux, Anna Lena"],["dc.contributor.author","Mazanec, Janine"],["dc.contributor.author","Strommenger, Birgit"],["dc.contributor.author","Hummel, Susanne"],["dc.date.accessioned","2019-07-09T11:44:36Z"],["dc.date.available","2019-07-09T11:44:36Z"],["dc.date.issued","2017"],["dc.description.abstract","Staphylococcus aureus is a major pathogen causing osteomyelitis, amongst other diseases, and its methicillin-resistant form (MRSA) in particular poses a huge threat to public health. To increase our knowledge of the origin and evolution of S. aureus, genetic studies of historical microorganisms may be beneficial. Thus, the aim of this study was to investigate whether osteomyelitic skeletal material (autopsy specimens collected from the mid 19th century until the 1920s) is suitable for detecting historical S. aureus DNA sequences. We established a PCR-based analysis system targeting two specific genes of S. aureus (nuc and fib). We successfully amplified the historical S. aureus nuc and fib sequences for six and seven pre-antibiotic, osteomyelitic bone specimens, respectively. These results encourage further investigations of historical S. aureus genomes that may increase our understanding of pathogen evolution in relation to anthropogenically introduced antibiotics."],["dc.identifier.doi","10.3390/d9040043"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14836"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59047"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1424-2818"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Staphylococcus aureus Sequences from Osteomyelitic Specimens of a Pathological Bone Collection from Pre-Antibiotic Times"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","48"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Diversity"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Haß, Fabian"],["dc.contributor.author","Hummel, Susanne"],["dc.contributor.author","Piskurek, Oliver"],["dc.date.accessioned","2019-07-09T11:44:37Z"],["dc.date.available","2019-07-09T11:44:37Z"],["dc.date.issued","2017"],["dc.description.abstract","The human-specific Alu elements, belonging to the class of Short INterspersed Elements (SINEs), have been shown to be a powerful tool for population genetic studies. An earlier study in this department showed that it was possible to analyze Alu presence/absence in 3000-year-old skeletal human remains from the Bronze Age Lichtenstein cave in Lower Saxony, Germany. We developed duplex Alu screening PCRs with flanking primers for two Alu elements, each combined with a single internal Alu primer. By adding an internal primer, the approximately 400–500 bp presence signals of Alu elements can be detected within a range of less than 200 bp. Thus, our PCR approach is suited for highly fragmented ancient DNA samples, whereas NGS analyses frequently are unable to handle repetitive elements. With this analysis system, we examined remains of 12 individuals from the Lichtenstein cave with different degrees of DNA degradation. The duplex PCRs showed fully informative amplification results for all of the chosen Alu loci in eight of the 12 samples. Our analysis system showed that Alu presence/absence analysis is possible in samples with different degrees of DNA degradation and it reduces the amount of valuable skeletal material needed by a factor of four, as compared with a singleplex approach."],["dc.identifier.doi","10.3390/d9040048"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14840"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59049"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","1424-2818"],["dc.relation.issn","1573-501X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Duplex Alu Screening for Degraded DNA of Skeletal Human Remains"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","574"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Forensic Sciences"],["dc.bibliographiccitation.lastpage","584"],["dc.bibliographiccitation.volume","2"],["dc.contributor.affiliation","Gupta, Avinash; 1Department of Historical Anthropology and Human Ecology, University of Göttingen, 37073 Göttingen, Germany"],["dc.contributor.affiliation","Billings, Brendon K.; 2Faculty of Health Sciences, School of Anatomical Sciences, University of the Witwatersrand, Parktown, Johannesburg 2193, South Africa"],["dc.contributor.affiliation","Hummel, Susanne; 1Department of Historical Anthropology and Human Ecology, University of Göttingen, 37073 Göttingen, Germany"],["dc.contributor.affiliation","Grosskopf, Birgit; 1Department of Historical Anthropology and Human Ecology, University of Göttingen, 37073 Göttingen, Germany"],["dc.contributor.author","Gupta, Avinash"],["dc.contributor.author","Billings, Brendon K."],["dc.contributor.author","Hummel, Susanne"],["dc.contributor.author","Grosskopf, Birgit"],["dc.date.accessioned","2022-11-15T16:47:53Z"],["dc.date.available","2022-11-15T16:47:53Z"],["dc.date.issued","2022-08-30"],["dc.date.updated","2022-11-11T13:14:56Z"],["dc.description.abstract","Objectives: The focus of this research is to evaluate the sex estimation methods on isolated human materials by applying morphological methods published in various forensic and anthropological literature on different skeletal series. Materials and Methods: 165 individuals from the 19th to 20th century Inden skeletal series, 252 individuals from the 13th to 14th century Lübeck skeletal series of German ancestry housed at the Department of Historical Anthropology and Human Ecology, the University of Göttingen, Germany, and 161 individuals from the 19th and 20th century of South African African ancestry housed within the Raymond A. Dart collection of modern human skeletons at the University of Witwatersrand, South Africa, with crania, mandibles, and pelves, were assessed. The evaluation criteria are burial information on the Inden series, genetic sex on both the Inden and the Lübeck series, and previous demography on cadavers from the South African African series. Results and Discussion: The sex estimation with cranial traits perform better in Inden and South Africa samples and worse in Lübeck sample. The mandible accuracies for pooled sexes are not exemplary, but the individual traits perform better for males in the Inden, Lübeck, and South Africa samples, except for gonion and angle, which performs better in females. The pelvic traits perform better in the Inden and South Africa samples compared to the Lübeck sample. The statistical tests show that there is a huge difference in the accuracy rates and the performance between both population groups from Germany itself, considering that Inden and Lübeck samples share the same ancestry. The accuracy rates improve with the exclusion of ambiguous individuals."],["dc.description.sponsorship","German Academic Exchange Service"],["dc.identifier.doi","10.3390/forensicsci2030042"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/117075"],["dc.language.iso","en"],["dc.relation.eissn","2673-6756"],["dc.title","Evaluating Morphological Methods for Sex Estimation on Isolated Human Skeletal Materials: Comparisons of Accuracies between German and South African Skeletal Collections"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1345"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Archaeological and Anthropological Sciences"],["dc.bibliographiccitation.lastpage","1357"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Olasz, Judit"],["dc.contributor.author","Seidenberg, Verena"],["dc.contributor.author","Hummel, Susanne"],["dc.contributor.author","Szentirmay, Zoltán"],["dc.contributor.author","Szabados, György"],["dc.contributor.author","Melegh, Béla"],["dc.contributor.author","Kásler, Miklós"],["dc.date.accessioned","2020-12-10T14:14:33Z"],["dc.date.available","2020-12-10T14:14:33Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1007/s12520-018-0609-7"],["dc.identifier.eissn","1866-9565"],["dc.identifier.issn","1866-9557"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15571"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71385"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","DNA profiling of Hungarian King Béla III and other skeletal remains originating from the Royal Basilica of Székesfehérvár"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e38132"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Buesse, Sebastian"],["dc.contributor.author","von Grumbkow, Philipp"],["dc.contributor.author","Hummel, Susanne"],["dc.contributor.author","Shah, Deep Narayan"],["dc.contributor.author","Shah, Ram Devi Tachamo"],["dc.contributor.author","Li, Jingke"],["dc.contributor.author","Zhang, X."],["dc.contributor.author","Yoshizawa, Kazunori"],["dc.contributor.author","Wedmann, Sonja"],["dc.contributor.author","Hoernschemeyer, Thomas"],["dc.date.accessioned","2018-11-07T09:10:13Z"],["dc.date.available","2018-11-07T09:10:13Z"],["dc.date.issued","2012"],["dc.description.abstract","Unusual biogeographic patterns of closely related groups reflect events in the past, and molecular analyses can help to elucidate these events. While ample research on the origin of disjunct distributions of different organism groups in the Western Paleartic has been conducted, such studies are rare for Eastern Palearctic organisms. In this paper we present a phylogeographic analysis of the disjunct distribution pattern of the extant species of the strongly cool-adapted Epiophlebia dragonflies from Asia. We investigated sequences of the usually more conserved 18 S rDNA and 28 S rDNA genes and the more variable sequences of ITS1, ITS2 and CO2 of all three currently recognised Epiophlebia species and of a sample of other odonatan species. In all genes investigated the degrees of similarity between species of Epiophlebia are very high and resemble those otherwise found between different populations of the same species in Odonata. This indicates that substantial gene transfer between these populations occurred in the comparatively recent past. Our analyses imply a wide distribution of the ancestor of extant Epiophlebia in Southeast Asia during the last ice age, when suitable habitats were more common. During the following warming phase, its range contracted, resulting in the current disjunct distribution. Given the strong sensitivity of these species to climatic parameters, the current trend to increasing global temperatures will further reduce acceptable habitats and seriously threaten the existences of these last representatives of an ancient group of Odonata."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2012"],["dc.identifier.doi","10.1371/journal.pone.0038132"],["dc.identifier.isi","000305353400087"],["dc.identifier.pmid","22666462"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7670"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26438"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Phylogeographic Analysis Elucidates the Influence of the Ice Ages on the Disjunct Distribution of Relict Dragonflies in Asia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]