Schmitz, Jessica E.

[["dc.bibliographiccitation.firstpage","153"],["dc.bibliographiccitation.lastpage","176"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Schmitz, Jessica"],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Walson, Philip D."],["dc.contributor.author","Schütz, Ekkehard"],["dc.contributor.editor","Oellerich, Michael"],["dc.contributor.editor","Dasgupta, Amitava"],["dc.date.accessioned","2020-05-22T06:58:17Z"],["dc.date.available","2020-05-22T06:58:17Z"],["dc.date.issued","2016"],["dc.description.abstract","Genome transplant dynamics is a particularly promising new approach for the detection of graft injury based on the determination of graft-derived circulating cell-free DNA (cfDNA) in the blood of the recipient. An increase of donor DNA is an early indication of organ damage. A novel potential routine assay for graft-derived circulating cfDNA quantification has been developed using droplet digital polymerase chain reaction for the determination of the donor/recipient circulating cfDNA ratio. This method is very cost-effective and provides results on the same day. Monitoring graft-derived cfDNA has the advantage that it directly interrogates the health of the donor organ, and it allows early detection of transplant injury (“liquid biopsy”). The detection of subclinical rejection would be desirable to allow early intervention. Undiagnosed chronic damage can result in chronic rejection. The determination of graft-derived circulating cfDNA may complement or possibly replace other approaches for post-transplant monitoring, and it may improve the chances of long-term graft survival. This method will be helpful to individualize immunosuppressive regimens. Personalized immunosuppression will in the future shift emphasis from reaction to prevention, which could make immunosuppressive drugs safer and more effective and also reduce the cost of health care."],["dc.identifier.doi","10.1016/B978-0-12-800885-0.00007-2"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/65676"],["dc.language.iso","en"],["dc.publisher","Elsevier"],["dc.publisher.place","San Diego"],["dc.relation.doi","10.1016/C2013-0-19247-1"],["dc.relation.isbn","978-0-12-800885-0"],["dc.relation.ispartof","Personalized Immunosuppression in Transplantation"],["dc.title","Graft-derived cell-free DNA as a marker of graft integrity after transplantation"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","78"],["dc.bibliographiccitation.journal","Peptides"],["dc.bibliographiccitation.lastpage","82"],["dc.bibliographiccitation.volume","58"],["dc.contributor.author","Holler, Julia Pia Natascha"],["dc.contributor.author","Schmitz, Jessica"],["dc.contributor.author","Roehrig, Rainer"],["dc.contributor.author","Wilker, Sigrid"],["dc.contributor.author","Hecker, Andreas"],["dc.contributor.author","Padberg, Winfried"],["dc.contributor.author","Grau, Veronika"],["dc.date.accessioned","2021-06-01T10:49:52Z"],["dc.date.available","2021-06-01T10:49:52Z"],["dc.date.issued","2014"],["dc.identifier.doi","10.1016/j.peptides.2014.05.009"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86445"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","0196-9781"],["dc.title","Expression of peptide YY by human blood leukocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3325"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","World Journal of Gastroenterology"],["dc.bibliographiccitation.lastpage","3329"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Brandhorst, Gunnar"],["dc.contributor.author","Petrova, Darinka Todorova"],["dc.contributor.author","Weigand, Sebastian"],["dc.contributor.author","Eberle, Christoph"],["dc.contributor.author","von Ahsen, Nicolas"],["dc.contributor.author","Schmitz, Jessica"],["dc.contributor.author","Schultze, Frank Christian"],["dc.contributor.author","Raddatz, Dirk"],["dc.contributor.author","Karaus, Michael"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Walson, Philip D."],["dc.date.accessioned","2018-11-07T09:59:29Z"],["dc.date.available","2018-11-07T09:59:29Z"],["dc.date.issued","2015"],["dc.description.abstract","AIM: To compare the number of regulatory T-cells (Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR (qPCR) method in patients suffering from inflammatory bowel disease (IBD). METHODS: Tregs percentages obtained by both flow cytometry and qPCR methods in 35 adult IBD patients, 18 out of them with Crohn's disease (CD) and 17 with ulcerative colitis (UC) were compared to each other as well as to scores on two IBD activity questionnaires using the Harvey Bradshaw Index (HBI) for CD patients and the Simple Colitis Clinical Activity Index (SCCAI) for UC patients. The Treg percentages by flow cytometry were defined as CD4(+)CD25(high)CD127(low)FOXP3(+) cells in peripheral blood mononuclear cells, whereas the Treg percentages by qPCR method were determined as FOXP3 promoter demethylation in genomic DNA. RESULTS: We found an average of 1.56% +/- 0.78% Tregs by using flow cytometry, compared to 1.07% +/- 0.53% Tregs by using qPCR in adult IBD patients. There were no significant correlations between either the percentages of Tregs measured by flow cytometry or qPCR and the HBI or SCCAI questionnaire scores in CD or UC patients, respectively. In addition, there was no correlation between Treg percentages measured by qPCR and those measured by flow cytometry (r = -0.06, P = 0.73; Spearman Rho). These data suggest that, either Treg-related immune function or the clinical scores in these IBD patients did not accurately reflect actual disease activity. Until the cause(s) for these differences are more clearly defined, the results suggest caution in interpreting studies of Tregs in various inflammatory disorders. CONCLUSION: The two methods did not produce equivalent measures of the percentage of total Tregs in the IBD patients studied which is consistent with the conclusion that Tregs subtypes are not equally detected by these two assays."],["dc.description.sponsorship","Medical Faculty of the University of Goettingen, Germany"],["dc.identifier.doi","10.3748/wjg.v21.i11.3325"],["dc.identifier.isi","000351165100021"],["dc.identifier.pmid","25805940"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11750"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37598"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Baishideng Publishing Group Inc"],["dc.relation.issn","2219-2840"],["dc.relation.issn","1007-9327"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.title","Lack of correlation between Treg quantification assays in inflammatory bowel disease patients"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S75"],["dc.bibliographiccitation.journal","Therapeutic Drug Monitoring"],["dc.bibliographiccitation.lastpage","S79"],["dc.bibliographiccitation.volume","38"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Walson, Philip D."],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Schmitz, Jessica"],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Schütz, Ekkehard"],["dc.date.accessioned","2020-12-10T18:19:52Z"],["dc.date.available","2020-12-10T18:19:52Z"],["dc.date.issued","2016"],["dc.description.abstract","Although short-term success after solid organ transplantation is good, long-term graft and recipient survival are both not satisfactory. Despite therapeutic drug monitoring (TDM) of immunosuppressive drugs (ISDs), both excessive and insufficient immunosuppression still do occur. There is a need for new biomarkers that, when combined with TDM, can be used to provide more effective and less toxic, personalized immunosuppression to improve long-term survival. Currently used methods are insufficient to rapidly, cost-effectively, and directly interrogate graft integrity after solid organ transplantation. However, because organ transplants are also genome transplants, measurement of graft-derived circulating cell-free DNA (GcfDNA) has shown promise as a way to improve both graft and recipient outcomes after solid organ transplantation through the early detection of severe graft injury, enabling an early intervention. A newly developed droplet digital polymerase chain reaction (ddPCR) method has advantages over expensive high-throughput sequencing methods to rapidly quantify GcfDNA percentages and absolute amounts. This procedure does not require donor DNA and therefore can be applied to any organ donor/recipient pair. The droplet digital polymerase chain reaction method allows for the early, sensitive, specific, and cost-effective direct assessment of graft integrity and can be used to define individual responses to ISDs including the minimal ISD exposures necessary to prevent rejection. This is especially important in patients undergoing ISD switches due to ISD toxicity, infections, or malignancies. Although prospective, multicenter clinical trials in liver, heart, and kidney transplantation have not been completed, early results suggest that GcfDNA can be combined with TDM to guide changes in immunosuppression to provide more effective, and less toxic treatment. Personalized immunosuppression will shift emphasis in transplantation from reaction to prevention and could improve outcome at lower health care costs."],["dc.identifier.doi","10.1097/FTD.0000000000000239"],["dc.identifier.isi","000377003400009"],["dc.identifier.issn","0163-4356"],["dc.identifier.pmid","26418703"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75409"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","1536-3694"],["dc.relation.issn","0163-4356"],["dc.title","Graft-Derived Cell-Free DNA as a Marker of Transplant Graft Injury"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","606"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Molecular Evolution"],["dc.bibliographiccitation.lastpage","612"],["dc.bibliographiccitation.volume","47"],["dc.contributor.author","Schmitz, J."],["dc.contributor.author","Moritz, R.F.A."],["dc.date.accessioned","2022-10-06T13:27:10Z"],["dc.date.available","2022-10-06T13:27:10Z"],["dc.date.issued","1998"],["dc.identifier.doi","10.1007/PL00006417"],["dc.identifier.pii","JME1776"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115270"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.issn","0022-2844"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Sociality and the Rate of rDNA Sequence Evolution in Wasps (Vespidae) and Honeybees (Apis)"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1732"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Clinical Chemistry"],["dc.bibliographiccitation.lastpage","1741"],["dc.bibliographiccitation.volume","59"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Bierau, Sarah"],["dc.contributor.author","Balzer, Stefan"],["dc.contributor.author","Andag, Reiner"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Schmitz, Jessica"],["dc.contributor.author","Gaedcke, Jochen"],["dc.contributor.author","Mörer, Onnen"],["dc.contributor.author","Slotta, Jan E."],["dc.contributor.author","Walson, Philip D."],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Schütz, Ekkehard"],["dc.date.accessioned","2020-05-22T07:07:40Z"],["dc.date.available","2020-05-22T07:07:40Z"],["dc.date.issued","2013"],["dc.description.abstract","BACKGROUND Cell-free DNA (cfDNA) from grafts in the circulation of transplant recipients is a potential biomarker of rejection. Its usefulness was investigated after heart transplantation during the maintenance phase by use of microarrays and massive parallel sequencing of donor and recipient DNA. Disadvantages of these methods are high costs, long turnaround times, and need for donor DNA. Therefore, we sought to develop a rapid and cost-effective method using digital droplet PCR (ddPCR). METHODS Plasma samples were collected from stable recipients after liver (LTx, n = 10), kidney (KTx, n = 9), and heart (HTx, n = 8) transplantation as well as from 7 additional patients directly after LTx. Known single-nucleotide polymorphisms were selected for high minor allelic frequencies, of which 41 hydrolysis probe assays were established. Plasma cfDNA was preamplified, followed by conventional real-time PCR to define informative (heterologous) SNPs, which were then used for quantification (percentage) of graft-derived cfDNA (GcfDNA) using ddPCR. RESULTS Mean recovery was 94% (SD, 13%) with an imprecision of 4%–14% with the use of controls with 2% minor allele. GcfDNA in stable patients was <6.8% (LTx), <2.5% (KTx), and <3.4% (HTx). On the day of LTx, GcfDNA was approximately 90% and by day 10 it was <15% in complication-free LTx recipients. In 2 patients with biopsy-proven rejection, GcfDNA increased to >60%, whereas in 1 patient with cholestasis no increase was found. CONCLUSIONS A novel, cost-effective, rapid technique was developed to quantify GcfDNA in transplant recipients. This technique embodies a promising, potentially universal biomarker for early detection of rejection, which could enable more effective therapeutic interventions."],["dc.identifier.doi","10.1373/clinchem.2013.210328"],["dc.identifier.pmid","24061615"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/65680"],["dc.language.iso","en"],["dc.relation.eissn","1530-8561"],["dc.relation.issn","0009-9147"],["dc.title","Digital droplet PCR for rapid quantification of donor DNA in the circulation of transplant recipients as a potential universal biomarker of graft injury"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S107"],["dc.bibliographiccitation.journal","Liver Transplantation"],["dc.bibliographiccitation.lastpage","S108"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Slotta, Jan Erik"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Birau, Sarah"],["dc.contributor.author","Balzer, Stefan"],["dc.contributor.author","Andag, Reiner"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Schmitz, Jessica"],["dc.contributor.author","Gaedcke, Jochen"],["dc.contributor.author","Moerer, Onnen"],["dc.contributor.author","Walson, Philip D."],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Schuetz, Eckehardt"],["dc.contributor.author","Kollmar, Otto"],["dc.date.accessioned","2018-11-07T09:39:07Z"],["dc.date.available","2018-11-07T09:39:07Z"],["dc.date.issued","2014"],["dc.identifier.isi","000339959601015"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33207"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.conference","Joint International Congress of ILTS, ELITA and LICAGE"],["dc.relation.eventlocation","London, ENGLAND"],["dc.relation.issn","1527-6473"],["dc.relation.issn","1527-6465"],["dc.title","Quantification of Donor DNA in the Circulation after Liver Transplantation As a Potential Universal Rejection Biomarker Using Digital Droplet PCR."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","e43"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Transplantation Journal"],["dc.bibliographiccitation.lastpage","e45"],["dc.bibliographiccitation.volume","98"],["dc.contributor.author","Kanzow, Philipp"],["dc.contributor.author","Kollmar, Otto"],["dc.contributor.author","Schütz, Ekkehard"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Schmitz, Jessica"],["dc.contributor.author","Beck, Julia"],["dc.contributor.author","Walson, Philip D."],["dc.contributor.author","Slotta, Jan Erik"],["dc.date.accessioned","2018-11-07T09:35:15Z"],["dc.date.accessioned","2020-05-22T07:54:43Z"],["dc.date.available","2018-11-07T09:35:15Z"],["dc.date.available","2020-05-22T07:54:43Z"],["dc.date.issued","2014"],["dc.identifier.isi","000341854800007"],["dc.identifier.pmid","25171533"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/65700"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.eissn","1534-6080"],["dc.relation.issn","0041-1337"],["dc.title","Graft-Derived Cell-Free DNA as an Early Organ Integrity Biomarker After Transplantation of a Marginal HELLP Syndrome Donor Liver"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","26"],["dc.bibliographiccitation.issue","1-3"],["dc.bibliographiccitation.journal","Cytogenetic and Genome Research"],["dc.bibliographiccitation.lastpage","37"],["dc.bibliographiccitation.volume","108"],["dc.contributor.author","Schmitz, J."],["dc.contributor.author","Roos, C."],["dc.contributor.author","Zischler, H."],["dc.date.accessioned","2022-10-06T13:25:56Z"],["dc.date.available","2022-10-06T13:25:56Z"],["dc.date.issued","2004"],["dc.description.abstract","In these postgenomic times where aspects of functional genetics and character evolution form a focal point of human-mouse comparative research, primate phylogenetic research gained a widespread interest in evolutionary biology. Nevertheless, it also remains a controversial subject. Despite the surge in available primate sequences and corresponding phylogenetic interpretations, primate origins as well as several branching events in primate divergence are far from settled. The analysis of SINEs – short interspersed elements – as molecular cladistic markers represents a particularly interesting complement to sequence data. The following summarizes and discusses potential applications of this new approach in molecular phylogeny and outlines main results obtained with SINEs in the context of primate evolutionary research. Another molecular cladistic marker linking the tarsier with the anthropoid primates is also presented. This eliminates any possibility of confounding phylogenetic interpretations through lineage sorting phenomena and makes use of a new point of view in settling the phylogenetic relationships of the primate infraorders.   "],["dc.identifier.doi","10.1159/000080799"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114951"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1424-859X"],["dc.relation.issn","1424-8581"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Primate phylogeny: molecular evidence from retroposons"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","434"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Applied Microbiology and Biotechnology"],["dc.bibliographiccitation.lastpage","442"],["dc.bibliographiccitation.volume","66"],["dc.contributor.author","Hoster, F."],["dc.contributor.author","Schmitz, J. E."],["dc.contributor.author","Daniel, Roy Thomas"],["dc.date.accessioned","2018-11-07T08:34:18Z"],["dc.date.available","2018-11-07T08:34:18Z"],["dc.date.issued","2005"],["dc.description.abstract","Thirteen different chitin-degrading bacteria were isolated from soil and sediment samples. Five of these strains (SGE2, SGE4, SSL3, MG1, and MG3) exhibited antifungal activity against phytopathogenic fungi. Analyses of the 16S rRNA genes and the substrate spectra revealed that the isolates belong to the genera Bacillus or Streptomyces. The closest relatives were Bacillus chitinolyticus ( SGE2, SGE4, and SSL3), B. ehimensis ( MG1), and Streptomyces griseus ( MG3). The chitinases present in the culture supernatants of the five isolates revealed optimal activity between 45 degreesC and 50 degreesC and at pH values of 4 ( SSL3), 5 ( SGE2 and MG1), 6 ( SGE4), and 5 - 7 ( MG3). The crude chitinase preparations of all five strains possessed antifungal activity. The chitinase of MG3 (ChiIS) was studied further, since the crude enzyme conferred strong growth suppression of all fungi tested and was very active over the entire pH range tested. The chiIS gene was cloned and the gene product was purified. The deduced protein consisted of 303 amino acids with a predicted molecular mass of 31,836 Da. Sequence analysis revealed that ChiIS of MG3 is similar to chitinases of Streptomyces species, which belong to family 19 of glycosyl hydrolases. Purified ChiIS showed remarkable antifungal activity and stability."],["dc.identifier.doi","10.1007/s00253-004-1664-9"],["dc.identifier.isi","000225716700014"],["dc.identifier.pmid","15290142"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17780"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-0614"],["dc.relation.issn","0175-7598"],["dc.title","Enrichment of chitinolytic microorganisms: isolation and characterization of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel Streptomyces strain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]