Schuh, Melina

[["dc.bibliographiccitation.firstpage","572"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Obstetrical & Gynecological Survey"],["dc.bibliographiccitation.lastpage","573"],["dc.bibliographiccitation.volume","70"],["dc.contributor.author","Holubcová, Zuzana"],["dc.contributor.author","Blayney, Martyn"],["dc.contributor.author","Elder, Kay"],["dc.contributor.author","Schuh, Melina"],["dc.date.accessioned","2022-03-01T11:46:52Z"],["dc.date.available","2022-03-01T11:46:52Z"],["dc.date.issued","2015"],["dc.identifier.doi","10.1097/OGX.0000000000000240"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103828"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0029-7828"],["dc.title","Error-Prone Chromosome-Mediated Spindle Assembly Favors Chromosome Segregation Defects in Human Oocytes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","eabj3944"],["dc.bibliographiccitation.issue","6581"],["dc.bibliographiccitation.journal","Science"],["dc.bibliographiccitation.volume","375"],["dc.contributor.author","So, Chun"],["dc.contributor.author","Menelaou, Katerina"],["dc.contributor.author","Uraji, Julia"],["dc.contributor.author","Harasimov, Katarina"],["dc.contributor.author","Steyer, Anna M."],["dc.contributor.author","Seres, K. Bianka"],["dc.contributor.author","Bucevičius, Jonas"],["dc.contributor.author","Lukinavičius, Gražvydas"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Sibold, Claus"],["dc.contributor.author","Tandler-Schneider, Andreas"],["dc.contributor.author","Eckel, Heike"],["dc.contributor.author","Moltrecht, Rüdiger"],["dc.contributor.author","Blayney, Martyn"],["dc.contributor.author","Elder, Kay"],["dc.contributor.author","Schuh, Melina"],["dc.date.accessioned","2022-02-22T15:58:13Z"],["dc.date.available","2022-02-22T15:58:13Z"],["dc.date.issued","2022"],["dc.description.abstract","Human oocytes are prone to assembling meiotic spindles with unstable poles, which can favor aneuploidy in human eggs. The underlying causes of spindle instability are unknown. We found that NUMA (nuclear mitotic apparatus protein)-mediated clustering of microtubule minus ends focused the spindle poles in human, bovine, and porcine oocytes and in mouse oocytes depleted of acentriolar microtubule-organizing centers (aMTOCs). However, unlike human oocytes, bovine, porcine, and aMTOC-free mouse oocytes have stable spindles. We identified the molecular motor KIFC1 (kinesin superfamily protein C1) as a spindle-stabilizing protein that is deficient in human oocytes. Depletion of KIFC1 recapitulated spindle instability in bovine and aMTOC-free mouse oocytes, and the introduction of exogenous KIFC1 rescued spindle instability in human oocytes. Thus, the deficiency of KIFC1 contributes to spindle instability in human oocytes."],["dc.identifier.doi","10.1126/science.abj3944"],["dc.identifier.pmid","35143306"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/100199"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/442"],["dc.identifier.url","https://for2848.gwdguser.de/literature/publications/5"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","FOR 2848: Architektur und Heterogenität der inneren mitochondrialen Membran auf der Nanoskala"],["dc.relation","FOR 2848 | P08: Strukturelle und funktionale Veränderungen der inneren mitochondrialen Membran axonaler Mitochondrien in vivo in einem dymyelinisierenden Mausmodell"],["dc.relation.eissn","1095-9203"],["dc.relation.issn","0036-8075"],["dc.relation.workinggroup","RG Möbius"],["dc.relation.workinggroup","RG Schuh"],["dc.title","Mechanism of spindle pole organization and instability in human oocytes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1143"],["dc.bibliographiccitation.issue","6239"],["dc.bibliographiccitation.journal","Science"],["dc.bibliographiccitation.lastpage","1147"],["dc.bibliographiccitation.volume","348"],["dc.contributor.author","Holubcová, Zuzana"],["dc.contributor.author","Blayney, Martyn"],["dc.contributor.author","Elder, Kay"],["dc.contributor.author","Schuh, Melina"],["dc.date.accessioned","2017-09-07T11:43:47Z"],["dc.date.available","2017-09-07T11:43:47Z"],["dc.date.issued","2015"],["dc.description.abstract","Aneuploidy in human eggs is the leading cause of pregnancy loss and several genetic disorders such as Down syndrome. Most aneuploidy results from chromosome segregation errors during the meiotic divisions of an oocyte, the egg's progenitor cell. The basis for particularly error-prone chromosome segregation in human oocytes is not known. We analyzed meiosis in more than 100 live human oocytes and identified an error-prone chromosome-mediated spindle assembly mechanism as a major contributor to chromosome segregation defects. Human oocytes assembled a meiotic spindle independently of either centrosomes or other microtubule organizing centers. Instead, spindle assembly wasmediated by chromosomes and the small guanosine triphosphatase Ran in a process requiring similar to 16 hours. This unusually long spindle assembly period was marked by intrinsic spindle instability and abnormal kinetochore-microtubule attachments, which favor chromosome segregation errors and provide a possible explanation for high rates of aneuploidy in human eggs."],["dc.identifier.doi","10.1126/science.aaa9529"],["dc.identifier.gro","3141889"],["dc.identifier.isi","000355590500050"],["dc.identifier.pmid","26045437"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2200"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1095-9203"],["dc.relation.issn","0036-8075"],["dc.title","Error-prone chromosome-mediated spindle assembly favors chromosome segregation defects in human oocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3749"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","Current Biology"],["dc.bibliographiccitation.lastpage","3765.e7"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Zielinska, Agata P."],["dc.contributor.author","Bellou, Eirini"],["dc.contributor.author","Sharma, Ninadini"],["dc.contributor.author","Frombach, Ann-Sophie"],["dc.contributor.author","Seres, K. Bianka"],["dc.contributor.author","Gruhn, Jennifer R."],["dc.contributor.author","Blayney, Martyn"],["dc.contributor.author","Eckel, Heike"],["dc.contributor.author","Moltrecht, Rüdiger"],["dc.contributor.author","Elder, Kay"],["dc.contributor.author","Schuh, Melina"],["dc.date.accessioned","2022-03-01T11:45:07Z"],["dc.date.available","2022-03-01T11:45:07Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.cub.2019.09.006"],["dc.identifier.pii","S0960982219311662"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103219"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0960-9822"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","Meiotic Kinetochores Fragment into Multiple Lobes upon Cohesin Loss in Aging Eggs"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e11389"],["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Zielinska, Agata P"],["dc.contributor.author","Holubcova, Zuzana"],["dc.contributor.author","Blayney, Martyn"],["dc.contributor.author","Elder, Kay"],["dc.contributor.author","Schuh, Melina"],["dc.date.accessioned","2017-09-07T11:54:48Z"],["dc.date.available","2017-09-07T11:54:48Z"],["dc.date.issued","2015"],["dc.description.abstract","Aneuploidy in human eggs is the leading cause of pregnancy loss and Downs syndrome. Aneuploid eggs result from chromosome segregation errors when an egg develops from a progenitor cell, called an oocyte. The mechanisms that lead to an increase in aneuploidy with advanced maternal age are largely unclear. Here, we show that many sister kinetochores in human oocytes are separated and do not behave as a single functional unit during the first meiotic division. Having separated sister kinetochores allowed bivalents to rotate by 90 degrees on the spindle and increased the risk of merotelic kinetochore-microtubule attachments. Advanced maternal age led to an increase in sister kinetochore separation, rotated bivalents and merotelic attachments. Chromosome arm cohesion was weakened, and the fraction of bivalents that precociously dissociated into univalents was increased. Together, our data reveal multiple age-related changes in chromosome architecture that could explain why oocyte aneuploidy increases with advanced maternal age."],["dc.format.extent","19"],["dc.identifier.doi","10.7554/eLife.11389"],["dc.identifier.gro","3141764"],["dc.identifier.isi","000373953000001"],["dc.identifier.pmid","26670547"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/813"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2050-084X"],["dc.title","Sister kinetochore splitting and precocious disintegration of bivalents could explain the maternal age effect"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Cavazza, Tommaso"],["dc.contributor.author","Politi, Antonio Z."],["dc.contributor.author","Aldag, Patrick"],["dc.contributor.author","Baker, Clara"],["dc.contributor.author","Elder, Kay"],["dc.contributor.author","Blayney, Martyn"],["dc.contributor.author","Lucas-Hahn, Andrea"],["dc.contributor.author","Niemann, Heiner"],["dc.contributor.author","Schuh, Melina"],["dc.date.accessioned","2022-02-23T13:16:11Z"],["dc.date.available","2022-02-23T13:16:11Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1101/2020.08.27.269779"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/100362"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/159"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Schuh"],["dc.title","Parental genome unification is highly erroneous in mammalian embryos"],["dc.type","preprint"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2860.e22"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Cell"],["dc.bibliographiccitation.lastpage","2877.e22"],["dc.bibliographiccitation.volume","184"],["dc.contributor.author","Cavazza, Tommaso"],["dc.contributor.author","Takeda, Yuko"],["dc.contributor.author","Politi, Antonio Z."],["dc.contributor.author","Aushev, Magomet"],["dc.contributor.author","Aldag, Patrick"],["dc.contributor.author","Baker, Clara"],["dc.contributor.author","Choudhary, Meenakshi"],["dc.contributor.author","Bucevičius, Jonas"],["dc.contributor.author","Lukinavičius, Gražvydas"],["dc.contributor.author","Elder, Kay"],["dc.contributor.author","Blayney, Martyn"],["dc.contributor.author","Lucas-Hahn, Andrea"],["dc.contributor.author","Niemann, Heiner"],["dc.contributor.author","Herbert, Mary"],["dc.contributor.author","Schuh, Melina"],["dc.date.accessioned","2022-02-22T07:42:17Z"],["dc.date.available","2022-02-22T07:42:17Z"],["dc.date.issued","2021"],["dc.description.abstract","Most human embryos are aneuploid. Aneuploidy frequently arises during the early mitotic divisions of the embryo, but its origin remains elusive. Human zygotes that cluster their nucleoli at the pronuclear interface are thought to be more likely to develop into healthy euploid embryos. Here, we show that the parental genomes cluster with nucleoli in each pronucleus within human and bovine zygotes, and clustering is required for the reliable unification of the parental genomes after fertilization. During migration of intact pronuclei, the parental genomes polarize toward each other in a process driven by centrosomes, dynein, microtubules, and nuclear pore complexes. The maternal and paternal chromosomes eventually cluster at the pronuclear interface, in direct proximity to each other, yet separated. Parental genome clustering ensures the rapid unification of the parental genomes on nuclear envelope breakdown. However, clustering often fails, leading to chromosome segregation errors and micronuclei, incompatible with healthy embryo development."],["dc.identifier.doi","10.1016/j.cell.2021.04.013"],["dc.identifier.pmid","33964210"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/100167"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/254"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1097-4172"],["dc.relation.issn","0092-8674"],["dc.relation.workinggroup","RG Schuh"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","Parental genome unification is highly error-prone in mammalian embryos"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]