Bohne, Wolfgang

[["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.volume","293"],["dc.contributor.author","Meyer, J."],["dc.contributor.author","Riebe, R."],["dc.contributor.author","Conraths, Franz J."],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Rohn, K."],["dc.contributor.author","Peters, M."],["dc.contributor.author","Schares, Gereon"],["dc.date.accessioned","2018-11-07T10:50:40Z"],["dc.date.available","2018-11-07T10:50:40Z"],["dc.date.issued","2004"],["dc.format.extent","110"],["dc.identifier.isi","000222589900176"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48708"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","21st Congress of the German-Society-of-Parasitology"],["dc.relation.eventlocation","Univ Wurzburg, Wurzburg, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Comparative examination of the expression of tissue cyst markers in Hammondia sp isolated from dogs and foxes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","229"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","International Journal for Parasitology"],["dc.bibliographiccitation.lastpage","234"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Schares, Gereon"],["dc.contributor.author","Meyer, J."],["dc.contributor.author","Barwald, A."],["dc.contributor.author","Conraths, Franz J."],["dc.contributor.author","Riebe, R."],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Rohn, K."],["dc.contributor.author","Peters, M."],["dc.date.accessioned","2018-11-07T10:40:24Z"],["dc.date.available","2018-11-07T10:40:24Z"],["dc.date.issued","2003"],["dc.description.abstract","Tissue cysts of parasites of the genus Hammondia are rarely described in naturally or experimentally infected intermediate hosts, However, ultrastructural examinations on tissue cyst stages of Hammondia sp. are needed, e.g. to compare these stages with those of Neospora caninum and other related parasites. We describe a cell culture system employed to examine the in vitro development of tissue cysts of a Hammondia sp.-like parasite (isolate FOX 2000/1) which uses the European fox as a definitive host. Cells of a diploid finite cell line from embryonal bovine heart (KH-R CCLV, RIE 090) were infected by inoculation of sporozoites und cultivated for up to 3 months. Transmission electron microscopic examination of 17 day old cell culture material revealed the presence of cyst walls. Infected cell cultures cultivated for 2 months were used to feed a fox. Six to 13 days post infection the fox shed large number,, (n = 1.2 X 10(7)) of Hammondia-sp. like oocysts which could not be distinguished from those used to infect the Cell Culture as determined by DNA sequencing of the internal transcribed spacer I and the D2/D3 domain of the large subunit ribosomal DNA, To find out the proportion of parasitophorous vacuoles that had developed into tissue cysts. the expression of bradyzoite markers was examined by probing infected cell cultures with Mouse polyclonal antibodies against Toxoplasma gondii bradyzoitc antigen I (anti-BAG1) and rat monoclonal antibodies against a cyst wall protein (mAbCC2). Nineteen and 90 days post infection all parasitophorous vacuoles in the cell cultures were positive with anti-BAG1 and mAbCC2. This shows that biologically viable (i.e. infectious) tissue cysts of a fox-derived Hammondia sp. isolate (FOX 2000/1) can be efficiently produced in this cell culture system. Since in Nitro cystogenesis of dog-derived Hammondia heydorni has not been observed et, in vitro cyst formation might be one trait to separate fox-derived Hammondia sp. from H. heydorni on a species level. (C) 2003 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved."],["dc.identifier.doi","10.1016/S0020-7519(03)00009-2"],["dc.identifier.isi","000182359100001"],["dc.identifier.pmid","12670509"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/46295"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Pergamon-elsevier Science Ltd"],["dc.relation.issn","0020-7519"],["dc.title","A Hammondia-like parasite from the European fox (Vulpes vulpes) forms biologically viable tissue cysts in cell culture"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","46"],["dc.bibliographiccitation.journal","Experimental Parasitology"],["dc.bibliographiccitation.lastpage","56"],["dc.bibliographiccitation.volume","163"],["dc.contributor.author","Gondim, Luis F. P."],["dc.contributor.author","Wolf, Alexander"],["dc.contributor.author","Vrhovec, M. Globokar"],["dc.contributor.author","Pantchev, Nikola"],["dc.contributor.author","Bauer, Christian R."],["dc.contributor.author","Langenmayer, Martin C."],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Teifke, Jens P."],["dc.contributor.author","Dubey, Jitender P."],["dc.contributor.author","Conraths, Franz J."],["dc.contributor.author","Schares, Gereon"],["dc.date.accessioned","2018-11-07T10:16:31Z"],["dc.date.available","2018-11-07T10:16:31Z"],["dc.date.issued","2016"],["dc.description.abstract","Toxoplasma gondii infects animals habiting terrestrial and aquatic environments. Its oocysts and tissue cysts are important for the horizontal transmission of this parasite. The oocyst and tissue cyst walls are crucial for the ability of the parasite to persist in the environment or in animal tissues, respectively. However, the composition of these walls is not well understood. We report the generation of monoclonal antibodies directed against wall components using mice immunized with oocyst antigens of T gondii. One monoclonal antibody (mAb) G1/19 reacted solely with T. gondii sporozoites. The respective antigen had a relative molecular weight (Mr) of 30 kDa. MAb G1/19 failed to react with sporozoites of any other coccidian parasite species tested (Hammondia hammondi, Hammondia heydorni, Cystoisospora felis, Eimeria bovis, Sarcocystis sp.). Another mAb, designated K8/15-15, recognized antigens in sporocyst walls of the parasite and in the walls of in vivo or in vitro produced tissue cysts, as demonstrated by immunofluorescence and immunoblot assays. Antigens of 80 to a high molecular weight protein of about 350 kDa Mr were recognized by this antibody using antigen extracts from sporocysts, and from in vitro or in vivo generated tissue cysts of the parasite. Tissue cyst and sporocyst walls of H. hammondi and H. heydorni, and tissue cysts of Neospora caninum were also recognized by mAb K8/15-15. Sporocyst walls of C. felis also reacted to this mAb. The cyst walls of Sarcocystis sp. and Besnoitia besnoiti were not recognized by mAb K8/15-15. Reactivity by a single mAb against T. gondii antigens in tissue cysts and sporocysts had not been reported previously. MAb K8/15-15 may be a practical tool for the identification of both cysts and sporocysts of the parasite, and may also be potentially employed in proteomic studies on the identification of new components of the cyst and sporocyst walls of T. gondii. (C) 2016 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.exppara.2016.01.014"],["dc.identifier.isi","000372667700007"],["dc.identifier.pmid","26836446"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41055"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","1090-2449"],["dc.relation.issn","0014-4894"],["dc.title","Characterization of an IgG monoclonal antibody targeted to both tissue cyst and sporocyst walls of Toxoplasma gondii"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]