Klopfenstein, Dieter R.

[["dc.bibliographiccitation.firstpage","4942"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","EMBO Journal"],["dc.bibliographiccitation.lastpage","4954"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Gerson-Gurwitz, Adina"],["dc.contributor.author","Thiede, Christina"],["dc.contributor.author","Movshovich, Natalia"],["dc.contributor.author","Fridman, Vladimir"],["dc.contributor.author","Podolskaya, Maria"],["dc.contributor.author","Danieli, Tsafi"],["dc.contributor.author","Lakaemper, Stefan"],["dc.contributor.author","Klopfenstein, Dieter R."],["dc.contributor.author","Schmidt, Christoph"],["dc.contributor.author","Gheber, Larisa"],["dc.date.accessioned","2017-09-07T11:43:13Z"],["dc.date.available","2017-09-07T11:43:13Z"],["dc.date.issued","2011"],["dc.description.abstract","Kinesin-5 motors fulfil essential roles in mitotic spindle morphogenesis and dynamics as slow, processive microtubule (MT) plus-end directed motors. The Saccharomyces cerevisiae kinesin-5 Cin8 was found, surprisingly, to switch directionality. Here, we have examined directionality using single-molecule fluorescence motility assays and live-cell microscopy. On spindles, Cin8 motors mostly moved slowly (similar to 25 nm/s) towards the midzone, but occasionally also faster (similar to 55 nm/s) towards the spindle poles. In vitro, individual Cin8 motors could be switched by ionic conditions from rapid (380 nm/s) and processive minus-end to slow plus-end motion on single MTs. At high ionic strength, Cin8 motors rapidly alternated directionalities between antiparallel MTs, while driving steady plus-end relative sliding. Between parallel MTs, plus-end motion was only occasionally observed. Deletion of the uniquely large insert in loop 8 of Cin8 induced bias towards minus-end motility and affected the ionic strength-dependent directional switching of Cin8 in vitro. The deletion mutant cells exhibited reduced midzone-directed motility and efficiency to support spindle elongation, indicating the importance of directionality control for the anaphase function of Cin8. The EMBO Journal (2011) 30, 4942-4954. doi: 10.1038/emboj.2011.403; Published online 18 November 2011"],["dc.identifier.doi","10.1038/emboj.2011.403"],["dc.identifier.gro","3142614"],["dc.identifier.isi","000298368900012"],["dc.identifier.pmid","22101328"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","0261-4189"],["dc.title","Directionality of individual kinesin-5 Cin8 motors is modulated by loop 8, ionic strength and microtubule geometry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","16841"],["dc.bibliographiccitation.issue","27"],["dc.bibliographiccitation.journal","Journal of biological chemistry"],["dc.bibliographiccitation.lastpage","16850"],["dc.bibliographiccitation.volume","290"],["dc.contributor.author","Dueselder, Andre"],["dc.contributor.author","Fridman, Vladimir"],["dc.contributor.author","Thiede, Christina"],["dc.contributor.author","Wiesbaum, Alice"],["dc.contributor.author","Goldstein, Alina"],["dc.contributor.author","Klopfenstein, Dieter R."],["dc.contributor.author","Zaitseva, Olga"],["dc.contributor.author","Janson, Marcel E."],["dc.contributor.author","Gheber, Larisa"],["dc.contributor.author","Schmidt, Christoph"],["dc.date.accessioned","2017-09-07T11:43:42Z"],["dc.date.available","2017-09-07T11:43:42Z"],["dc.date.issued","2015"],["dc.description.abstract","The bipolar kinesin-5 motors are one of the major players that govern mitotic spindle dynamics. Their bipolar structure enables them to cross-link and slide apart antiparallel microtubules (MTs) emanating from the opposing spindle poles. The budding yeast kinesin-5 Cin8 was shown to switch from fast minus-end-to slow plus-end-directed motility upon binding between antiparallel MTs. This unexpected finding revealed a new dimension of cellular control of transport, the mechanism of which is unknown. Here we have examined the role of the C-terminal tail domain of Cin8 in regulating directionality. We first constructed a stable dimeric Cin8/kinesin-1 chimera (Cin8Kin), consisting of head and neck linker of Cin8 fused to the stalk of kinesin-1. As a single dimeric motor, Cin8Kin switched frequently between plus and minus directionality along single MTs, demonstrating that the Cin8 head domains are inherently bidirectional, but control over directionality was lost. We next examined the activity of a tetrameric Cin8 lacking only the tail domains (Cin8 Delta tail). In contrast to wild-type Cin8, the motility of single molecules of Cin8 Delta tail in high ionic strength was slow and bidirectional, with almost no directionality switches. Cin8 Delta tail showed only a weak ability to cross-link MTs in vitro. In vivo, Cin8 Delta tail exhibited bias toward the plus-end of the MTs and was unable to support viability of cells as the sole kinesin-5 motor. We conclude that the tail of Cin8 is not necessary for bidirectional processive motion, but is controlling the switch between plus-and minus-end-directed motility."],["dc.identifier.doi","10.1074/jbc.M114.620799"],["dc.identifier.gro","3141866"],["dc.identifier.isi","000357572800029"],["dc.identifier.pmid","25991727"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1945"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Amer Soc Biochemistry Molecular Biology Inc"],["dc.relation.eissn","1083-351X"],["dc.relation.issn","0021-9258"],["dc.title","Deletion of the Tail Domain of the Kinesin-5 Cin8 Affects Its Directionality"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Gerson-Gurwitz, Adina"],["dc.contributor.author","Thiede, Christina"],["dc.contributor.author","Movshovich, Natalia"],["dc.contributor.author","Fridman, Vladimir"],["dc.contributor.author","Podolskaya, Maria"],["dc.contributor.author","Lakaemper, Stefan"],["dc.contributor.author","Klopfenstein, Dieter R."],["dc.contributor.author","Schmidt, Christoph F."],["dc.contributor.author","Gheber, Larisa"],["dc.date.accessioned","2018-11-07T09:14:14Z"],["dc.date.available","2018-11-07T09:14:14Z"],["dc.date.issued","2012"],["dc.format.extent","696A"],["dc.identifier.doi","10.1016/j.bpj.2011.11.3778"],["dc.identifier.isi","000321561204689"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27361"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.conference","56th Annual Meeting of the Biophysical-Society"],["dc.relation.eventlocation","San Diego, CA"],["dc.relation.issn","0006-3495"],["dc.title","Control of Directionality of Individual Kinesin-5 Motors"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Gurwitz, Adina Gerson"],["dc.contributor.author","Thiede, Christina"],["dc.contributor.author","Movshovich, Natalia"],["dc.contributor.author","Fridman, Vladimir"],["dc.contributor.author","Podolskaya, Maria"],["dc.contributor.author","Lakaemper, Stefan"],["dc.contributor.author","Klopfenstein, Dieter Robert"],["dc.contributor.author","Schmidt, Christoph F."],["dc.contributor.author","Gheber, Larisa"],["dc.date.accessioned","2018-11-07T09:14:14Z"],["dc.date.available","2018-11-07T09:14:14Z"],["dc.date.issued","2012"],["dc.format.extent","702A"],["dc.identifier.isi","000321561205018"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27362"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.conference","56th Annual Meeting of the Biophysical-Society"],["dc.relation.eventlocation","San Diego, CA"],["dc.relation.issn","0006-3495"],["dc.title","Loop 8 Plays a Role in Controlling S. Cerevisiae Kinesin-5 Cin8 Motility and Function"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","Gerson-Gurwitz, Adina"],["dc.contributor.author","Thiede, Christina"],["dc.contributor.author","Movshovich, Natalia"],["dc.contributor.author","Fridman, Vladimir"],["dc.contributor.author","Podolskaya, Maria"],["dc.contributor.author","Danieli, Tsafi"],["dc.contributor.author","Lakaemper, Stefan"],["dc.contributor.author","Klopfenstein, Dieter Robert"],["dc.contributor.author","Schmidt, Christoph F."],["dc.contributor.author","Gheber, Larisa"],["dc.date.accessioned","2018-11-07T08:53:36Z"],["dc.date.available","2018-11-07T08:53:36Z"],["dc.date.issued","2011"],["dc.format.extent","163"],["dc.identifier.isi","000293637300425"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22455"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.conference","8th EBSA European Biophysics Congress"],["dc.relation.eventlocation","Budapest, HUNGARY"],["dc.relation.issn","0175-7571"],["dc.title","Control of the fast, slow and reverse gear of the yeast mitotic kinesin-5 Cin8"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]