Reichmann, Jakob

[["dc.bibliographiccitation.artnumber","259"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Communications Physics"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Shaker, Kian"],["dc.contributor.author","Häggmark, Ilian"],["dc.contributor.author","Reichmann, Jakob"],["dc.contributor.author","Arsenian-Henriksson, Marie"],["dc.contributor.author","Hertz, Hans M."],["dc.date.accessioned","2022-01-11T14:05:44Z"],["dc.date.available","2022-01-11T14:05:44Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Phase-contrast X-ray lung imaging has broken new ground in preclinical respiratory research by improving contrast at air/tissue interfaces. To minimize blur from respiratory motion, intubation and mechanical ventilation is commonly employed for end-inspiration gated imaging at synchrotrons and in the laboratory. Inevitably, the prospect of ventilation induced lung injury (VILI) renders mechanical ventilation a confounding factor in respiratory studies of animal models. Here we demonstrate proof-of-principle 3D imaging of the tracheobronchial tree in free-breathing mice without mechanical ventilation at radiation levels compatible with longitudinal studies. We use a prospective gating approach for end-expiration propagation-based phase-contrast X-ray imaging where the natural breathing of the mouse dictates the acquisition flow. We achieve intrapulmonary spatial resolution in the 30-μm-range, sufficient for resolving terminal bronchioles in the 60-μm-range distinguished from the surrounding lung parenchyma. These results should enable non-invasive longitudinal studies of native state murine airways for translational lung disease research in the laboratory."],["dc.description.abstract","Abstract Phase-contrast X-ray lung imaging has broken new ground in preclinical respiratory research by improving contrast at air/tissue interfaces. To minimize blur from respiratory motion, intubation and mechanical ventilation is commonly employed for end-inspiration gated imaging at synchrotrons and in the laboratory. Inevitably, the prospect of ventilation induced lung injury (VILI) renders mechanical ventilation a confounding factor in respiratory studies of animal models. Here we demonstrate proof-of-principle 3D imaging of the tracheobronchial tree in free-breathing mice without mechanical ventilation at radiation levels compatible with longitudinal studies. We use a prospective gating approach for end-expiration propagation-based phase-contrast X-ray imaging where the natural breathing of the mouse dictates the acquisition flow. We achieve intrapulmonary spatial resolution in the 30-μm-range, sufficient for resolving terminal bronchioles in the 60-μm-range distinguished from the surrounding lung parenchyma. These results should enable non-invasive longitudinal studies of native state murine airways for translational lung disease research in the laboratory."],["dc.identifier.doi","10.1038/s42005-021-00760-8"],["dc.identifier.pii","760"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97738"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation.eissn","2399-3650"],["dc.title","Phase-contrast X-ray tomography resolves the terminal bronchioles in free-breathing mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]