Chizhik, Anna M.

[["dc.bibliographiccitation.artnumber","204201"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","The Journal of Chemical Physics"],["dc.bibliographiccitation.volume","148"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Stein, Simon C"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2020-05-29T09:29:43Z"],["dc.date.available","2020-05-29T09:29:43Z"],["dc.date.issued","2018-05-28"],["dc.description.abstract","Our paper presents the first theoretical and experimental study using single-molecule Metal-Induced Energy Transfer (smMIET) for localizing single fluorescent molecules in three dimensions. Metal-Induced Energy Transfer describes the resonant energy transfer from the excited state of a fluorescent emitter to surface plasmons in a metal nanostructure. This energy transfer is strongly distance-dependent and can be used to localize an emitter along one dimension. We have used Metal-Induced Energy Transfer in the past for localizing fluorescent emitters with nanometer accuracy along the optical axis of a microscope. The combination of smMIET with single-molecule localization based super-resolution microscopy that provides nanometer lateral localization accuracy offers the prospect of achieving isotropic nanometer localization accuracy in all three spatial dimensions. We give a thorough theoretical explanation and analysis of smMIET, describe its experimental requirements, also in its combination with lateral single-molecule localization techniques, and present first proof-of-principle experiments using dye molecules immobilized on top of a silica spacer, and of dye molecules embedded in thin polymer films."],["dc.identifier.doi","10.1063/1.5027074"],["dc.identifier.pmid","29865842"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66012"],["dc.language.iso","en"],["dc.relation.eissn","1089-7690"],["dc.relation.issn","0021-9606"],["dc.title","Three-dimensional single-molecule localization with nanometer accuracy using Metal-Induced Energy Transfer (MIET) imaging"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","11839"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","11846"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Pfaff, Janine"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Kehlenbach, Ralph H."],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2018-04-23T11:48:40Z"],["dc.date.available","2018-04-23T11:48:40Z"],["dc.date.issued","2017"],["dc.description.abstract","The nuclear envelope, comprising the inner and the outer nuclear membrane, separates the nucleus from the cytoplasm and plays a key role in cellular functions. Nuclear pore complexes (NPCs), which are embedded in the nuclear envelope, control transport of macromolecules between the two compartments. Here, using dual-color metal-induced energy transfer (MIET), we determine the axial distance between Lap2β and Nup358 as markers for the inner nuclear membrane and the cytoplasmic side of the NPC, respectively. Using MIET imaging, we reconstruct the 3D profile of the nuclear envelope over the whole basal area, with an axial resolution of a few nanometers. This result demonstrates that optical microscopy can achieve nanometer axial resolution in biological samples and without recourse to complex interferometric approaches."],["dc.identifier.doi","10.1021/acsnano.7b04671"],["dc.identifier.gro","3142097"],["dc.identifier.pmid","28921961"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13555"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/13"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P07: Dynamik von Proteinen der inneren Kernmembran"],["dc.relation.issn","1936-0851"],["dc.relation.workinggroup","RG Kehlenbach (Nuclear Transport)"],["dc.title","Three-Dimensional Reconstruction of Nuclear Envelope Architecture Using Dual-Color Metal-Induced Energy Transfer Imaging"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","265"],["dc.bibliographiccitation.lastpage","281"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Chizhik, Alexey I."],["dc.date.accessioned","2020-05-29T09:29:20Z"],["dc.date.available","2020-05-29T09:29:20Z"],["dc.date.issued","2014"],["dc.identifier.doi","10.1007/4243_2014_77"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66008"],["dc.relation.isbn","978-3-319-15635-4"],["dc.relation.isbn","978-3-319-15636-1"],["dc.relation.ispartof","Advanced Photon Counting"],["dc.title","Metal-Induced Energy Transfer"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","846"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.lastpage","851"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Chizhik, Anna M."],["dc.contributor.author","Wollnik, Carina"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Hauke, Lara"],["dc.contributor.author","Hähnel, Dirk"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Rehfeldt, Florian"],["dc.contributor.editor","Bassereau, Patricía"],["dc.date.accessioned","2020-12-10T18:16:05Z"],["dc.date.available","2020-12-10T18:16:05Z"],["dc.date.issued","2018"],["dc.description.abstract","We report a novel method, dual-color axial nanometric localization by metal--induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonstrate the capability of this method on cytoskeletal elements and adhesions in human mesenchymal stem cells. Our approach is based on fluorescence-lifetime-imaging microscopy and allows for precise determination of the three-dimensional architecture of stress fibers anchoring at focal adhesions, thus yielding crucial information to understand cell-matrix mechanics. In addition to resolving nanometric structural details along the z-axis, we use FRET to gain precise information on the distance between actin and vinculin at focal adhesions."],["dc.identifier.doi","10.1091/mbc.E17-05-0314"],["dc.identifier.eissn","1939-4586"],["dc.identifier.issn","1059-1524"],["dc.identifier.pmid","29444956"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75048"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.eissn","1939-4586"],["dc.relation.issn","1059-1524"],["dc.relation.issn","1939-4586"],["dc.title","Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]