Kohl, Tobias

[["dc.bibliographiccitation.firstpage","318a"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","106"],["dc.contributor.author","Walker, Mark A."],["dc.contributor.author","Williams, George S. B."],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Jafri, Saleet"],["dc.contributor.author","Lehnart, Stephan E."],["dc.contributor.author","Greenstein, Joseph L."],["dc.contributor.author","Lederer, W. J."],["dc.contributor.author","Winslow, Raimond L."],["dc.date.accessioned","2022-03-01T11:44:58Z"],["dc.date.available","2022-03-01T11:44:58Z"],["dc.date.issued","2014"],["dc.identifier.doi","10.1016/j.bpj.2013.11.1839"],["dc.identifier.pii","S000634951303097X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103175"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","Super-Resolution Modeling of Calcium Release in Heart"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","305a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Lauterbach, Marcel"],["dc.contributor.author","Minh Tuan, Hoang-Trong"],["dc.contributor.author","Williams, George S.B."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Jafri, M. Saleet"],["dc.contributor.author","Lederer, W.J."],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2022-03-01T11:44:54Z"],["dc.date.available","2022-03-01T11:44:54Z"],["dc.date.issued","2012"],["dc.identifier.doi","10.1016/j.bpj.2011.11.1683"],["dc.identifier.pii","S0006349511030311"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103157"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","STED Nanoscopy of Cardiac RyR2 Clusters and Sub-Structure Analysis After Myocardial Infarction"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","The Journal of General Physiology"],["dc.bibliographiccitation.volume","136"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Wagner, Edward J."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hagen, Brian M."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T08:41:47Z"],["dc.date.available","2018-11-07T08:41:47Z"],["dc.date.issued","2010"],["dc.format.extent","9A"],["dc.identifier.isi","000279473500028"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19548"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Rockefeller Univ Press"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Marine Biol Lab, Woods Hole, MA"],["dc.relation.issn","0022-1295"],["dc.title","Super-Resolution Imaging of Cardiac Signaling Microdomains by STED Microscopy"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","106"],["dc.contributor.author","Walker, Mark A."],["dc.contributor.author","Williams, George S. B."],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Jafri, Saleet"],["dc.contributor.author","Lehnart, Stephan E."],["dc.contributor.author","Greenstein, Joseph L."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Winslow, Raimond L."],["dc.date.accessioned","2018-11-07T09:44:57Z"],["dc.date.available","2018-11-07T09:44:57Z"],["dc.date.issued","2014"],["dc.format.extent","318A"],["dc.identifier.isi","000337000401734"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34510"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","San Francisco, CA"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Super-Resolution Modeling of Calcium Release in Heart"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","223A"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","224A"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Wagner, Eva M."],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Williams, George S. B."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Streich, Jan-Hendrik"],["dc.contributor.author","Tuan, Hoang-Trong M."],["dc.contributor.author","Hagen, Brian M."],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Jafri, Mohsin S."],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T09:14:13Z"],["dc.date.available","2018-11-07T09:14:13Z"],["dc.date.issued","2012"],["dc.identifier.isi","000321561201431"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27356"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","San Diego, CA"],["dc.relation.issn","0006-3495"],["dc.title","Live Cell Super-Resolution Imaging of Transverse Membrane Tubules in Heart Failure"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","402"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","414"],["dc.bibliographiccitation.volume","111"],["dc.contributor.author","Wagner, Eva"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Williams, George S. B."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Streich, Jan-Hendrik"],["dc.contributor.author","Korff, Brigitte"],["dc.contributor.author","Tuan, Hoang-Trong M."],["dc.contributor.author","Hagen, Brian"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Jafri, M. Saleet"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2017-09-07T11:48:29Z"],["dc.date.available","2017-09-07T11:48:29Z"],["dc.date.issued","2012"],["dc.description.abstract","Rationale: Transverse tubules (TTs) couple electric surface signals to remote intracellular Ca2+ release units (CRUs). Diffraction-limited imaging studies have proposed loss of TT components as disease mechanism in heart failure (HF). Objectives: Objectives were to develop quantitative super-resolution strategies for live-cell imaging of TT membranes in intact cardiomyocytes and to show that TT structures are progressively remodeled during HF development, causing early CRU dysfunction. Methods and Results: Using stimulated emission depletion (STED) microscopy, we characterized individual TTs with nanometric resolution as direct readout of local membrane morphology 4 and 8 weeks after myocardial infarction (4pMI and 8pMI). Both individual and network TT properties were investigated by quantitative image analysis. The mean area of TT cross sections increased progressively from 4pMI to 8pMI. Unexpectedly, intact TT networks showed differential changes. Longitudinal and oblique TTs were significantly increased at 4pMI, whereas transversal components appeared decreased. Expression of TT-associated proteins junctophilin-2 and caveolin-3 was significantly changed, correlating with network component remodeling. Computational modeling of spatial changes in HF through heterogeneous TT reorganization and RyR2 orphaning (5000 of 20 000 CRUs) uncovered a local mechanism of delayed subcellular Ca2+ release and action potential prolongation. Conclusions: This study introduces STED nanoscopy for live mapping of TT membrane structures. During early HF development, the local TT morphology and associated proteins were significantly altered, leading to differential network remodeling and Ca2+ release dyssynchrony. Our data suggest that TT remodeling during HF development involves proliferative membrane changes, early excitation-contraction uncoupling, and network fracturing."],["dc.identifier.doi","10.1161/CIRCRESAHA.112.274530"],["dc.identifier.gro","3142487"],["dc.identifier.isi","000307308700007"],["dc.identifier.pmid","22723297"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8829"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/73"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A05: Molekulares Imaging von kardialen Calcium-Freisetzungsdomänen"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation.issn","0009-7330"],["dc.relation.workinggroup","RG Hasenfuß (Transition zur Herzinsuffizienz)"],["dc.relation.workinggroup","RG Hell"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.title","Stimulated Emission Depletion Live-Cell Super-Resolution Imaging Shows Proliferative Remodeling of T-Tubule Membrane Structures After Myocardial Infarction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","267a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Brandenburg, Sören"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Williams, George S.B."],["dc.contributor.author","Gusev, Konstantin"],["dc.contributor.author","Wagner, Eva"],["dc.contributor.author","Hebisch, Elke"],["dc.contributor.author","Ward, Christopher W."],["dc.contributor.author","Lederer, W.J."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2020-12-10T14:22:41Z"],["dc.date.available","2020-12-10T14:22:41Z"],["dc.date.issued","2016"],["dc.format.extent","267A"],["dc.identifier.doi","10.1016/j.bpj.2015.11.1454"],["dc.identifier.isi","000375141600298"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71697"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","Los Angeles, CA"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Axial Membrane Tubules in Atrial Cardiomyocytes Confine Ultrarapid Intracellular Calcium Signals through a New Super-Hub Mechanism"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e51823"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","92"],["dc.bibliographiccitation.journal","Journal of Visualized Experiments"],["dc.bibliographiccitation.lastpage","19"],["dc.contributor.author","Wagner, Eva"],["dc.contributor.author","Brandenburg, Sören"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Lehnart, Stephan Elmar"],["dc.date.accessioned","2018-05-07T12:58:36Z"],["dc.date.available","2018-05-07T12:58:36Z"],["dc.date.issued","2014"],["dc.description.abstract","In cardiac myocytes a complex network of membrane tubules--the transverse-axial tubule system (TATS)--controls deep intracellular signaling functions. While the outer surface membrane and associated TATS membrane components appear to be continuous, there are substantial differences in lipid and protein content. In ventricular myocytes (VMs), certain TATS components are highly abundant contributing to rectilinear tubule networks and regular branching 3D architectures. It is thought that peripheral TATS components propagate action potentials from the cell surface to thousands of remote intracellular sarcoendoplasmic reticulum (SER) membrane contact domains, thereby activating intracellular Ca(2+) release units (CRUs). In contrast to VMs, the organization and functional role of TATS membranes in atrial myocytes (AMs) is significantly different and much less understood. Taken together, quantitative structural characterization of TATS membrane networks in healthy and diseased myocytes is an essential prerequisite towards better understanding of functional plasticity and pathophysiological reorganization. Here, we present a strategic combination of protocols for direct quantitative analysis of TATS membrane networks in living VMs and AMs. For this, we accompany primary cell isolations of mouse VMs and/or AMs with critical quality control steps and direct membrane staining protocols for fluorescence imaging of TATS membranes. Using an optimized workflow for confocal or superresolution TATS image processing, binarized and skeletonized data are generated for quantitative analysis of the TATS network and its components. Unlike previously published indirect regional aggregate image analysis strategies, our protocols enable direct characterization of specific components and derive complex physiological properties of TATS membrane networks in living myocytes with high throughput and open access software tools. In summary, the combined protocol strategy can be readily applied for quantitative TATS network studies during physiological myocyte adaptation or disease changes, comparison of different cardiac or skeletal muscle cell types, phenotyping of transgenic models, and pharmacological or therapeutic interventions."],["dc.identifier.doi","10.3791/51823"],["dc.identifier.pmid","25350293"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/14630"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/69"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A05: Molekulares Imaging von kardialen Calcium-Freisetzungsdomänen"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation.doi","10.3791/51823"],["dc.relation.eissn","1940-087X"],["dc.relation.issn","1940-087X"],["dc.relation.workinggroup","RG Brandenburg"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.title","Analysis of tubular membrane networks in cardiac myocytes from atria and ventricles"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Hoang-Trong Minh Tuan, Hoang-Trong Minh Tuan"],["dc.contributor.author","Williams, George S. B."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Jafri, Mohsin S."],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T09:14:13Z"],["dc.date.available","2018-11-07T09:14:13Z"],["dc.date.issued","2012"],["dc.format.extent","305A"],["dc.identifier.isi","000321561202133"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27357"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","San Diego, CA"],["dc.relation.issn","0006-3495"],["dc.title","STED Nanoscopy of Cardiac RyR2 Clusters and Sub-Structure Analysis After Myocardial Infarction"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","104"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Wagner, Eva M."],["dc.contributor.author","Brandenburg, Soeren"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Lederer, W. Jonathan"],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T09:28:59Z"],["dc.date.available","2018-11-07T09:28:59Z"],["dc.date.issued","2013"],["dc.format.extent","340A"],["dc.identifier.isi","000316074303240"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30916"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","Philadelphia, PA"],["dc.relation.issn","0006-3495"],["dc.title","Principles of Network Organization of T-Tubule Membranes in Health and Disease"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]