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Bahlmann, Karsten
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Bahlmann, Karsten
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Bahlmann, Karsten
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Bahlmann, K.
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1995Journal Article [["dc.bibliographiccitation.firstpage","RP1"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","J. Microsc."],["dc.bibliographiccitation.lastpage","RP2"],["dc.bibliographiccitation.volume","180"],["dc.contributor.author","Hell, S. W."],["dc.contributor.author","Schrader, M."],["dc.contributor.author","Bahlmann, K."],["dc.contributor.author","Meinecke, F."],["dc.contributor.author","Lakowicz, J. R ."],["dc.contributor.author","Gryczynski, I."],["dc.date.accessioned","2018-03-21T14:33:54Z"],["dc.date.available","2018-03-21T14:33:54Z"],["dc.date.issued","1995"],["dc.description.abstract","We report the realization of stimulated emission on a microscopic scale. An experiment is presented describing significant depopulation of the excited state of the fluorophore Pyridine 2 with a mode‐locked Ti: Sapphire laser. Stimulated emission is performed at 750 nm and excitation at the frequency doubled wavelength of 375 nm. The pulses are synchronized so that the stimulating pulse follows the excitation pulse after 5 ps. The set‐up is a modified 4Pi‐confocal microscope employing one of the objective lenses for excitation and the opposing one for stimulated emission."],["dc.identifier.doi","10.1111/j.1365-2818.1995.tb03662.x"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13111"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.notes.status","final"],["dc.title","Stimulated Emission on a Microscopic Scale: Light Quenching of Pyridinium 2 using a Ti:Sapphire laser"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]Details DOI1997Journal Article Research Paper [["dc.bibliographiccitation.firstpage","116"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Optik"],["dc.bibliographiccitation.lastpage","124"],["dc.bibliographiccitation.volume","104"],["dc.contributor.author","Schrader, M."],["dc.contributor.author","Bahlmann, K."],["dc.contributor.author","Hell, S. W."],["dc.date.accessioned","2017-09-07T11:51:04Z"],["dc.date.available","2017-09-07T11:51:04Z"],["dc.date.issued","1997"],["dc.description.abstract","We derive the three-dimensional resolution of three-photon excitation fluorescence microscopy and compare it with its single and two-photon excitation counterpart. We perform three-photon excitation microscopy in the 900-970 nm range and compare the experimentally determined axial resolution with that of two-photon excitation microscopy at the same excitation wavelength. At an excitation wavelength of 900 nm, we measure a resolution increase of 35% of three-photon over two-photon excitation microscopy. This is in good agreement with the theoretically predicted resolution increase. At 900 nm and an aperture of 1.4 oil immersion we obtain an axial resolution of 587 +/- 27 nm. Three-photon images are displayed of DAPI-labelled metaphase chromosomes. The images are recorded by using a train of subpicosecond pulses of a mode-locked Titanium-Sapphire laser emitting at a wavelength of 970 nm. We also discuss the role of the pulse length and that of point out the implications of three-photon excitation for other far-field fluorescence microscopies. Our findings also reveal the potential of superresolution for increasing the information density in three-photon induced three-dimensional data storage."],["dc.identifier.gro","3144624"],["dc.identifier.isi","A1997WG26800006"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2269"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0030-4026"],["dc.title","Three-photon-excitation microscopy: Theory, experiment and applications"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]Details WOS1995Journal Article [["dc.bibliographiccitation.firstpage","RP1"],["dc.bibliographiccitation.journal","Journal of microscopy"],["dc.bibliographiccitation.lastpage","RP2"],["dc.bibliographiccitation.volume","180"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Schrader, M."],["dc.contributor.author","Bahlmann, K."],["dc.contributor.author","MEINECKE, F"],["dc.contributor.author","Lakowicz, J. R ."],["dc.contributor.author","Gryczynski, I."],["dc.date.accessioned","2017-09-07T11:51:11Z"],["dc.date.available","2017-09-07T11:51:11Z"],["dc.date.issued","1995"],["dc.description.abstract","We report the realization of stimulated emission on a microscopic scale. An experiment is presented describing significant depopulation of the excited state of the fluorophore Pyridine 2 with a mode-locked Ti:Sapphire laser, Stimulated emission is performed at 750 nm and excitation at the frequency doubled wavelength of 375 nm. The pulses are synchronized so that the stimulating pulse follows the excitation pulse after 5 ps. The set-up is a modified 4Pi-confocal microscope employing one of the objective lenses for excitation and the opposing one for stimulated emission."],["dc.identifier.gro","3144674"],["dc.identifier.isi","A1995TJ43100015"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2324"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0022-2720"],["dc.title","STIMULATED-EMISSION ON MICROSCOPIC SCALE - LIGHT QUENCHING OF PYRIDINE-2 USING A TI-SAPPHIRE LASER"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]Details WOS