Großhans, Jörg

[["dc.bibliographiccitation.firstpage","1187"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Current Biology"],["dc.bibliographiccitation.lastpage","1192.e3"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Liu, Boyang"],["dc.contributor.author","Winkler, Franziska"],["dc.contributor.author","Herde, Marco"],["dc.contributor.author","Witte, Claus-Peter"],["dc.contributor.author","Großhans, Jörg"],["dc.date.accessioned","2020-12-10T14:23:22Z"],["dc.date.available","2020-12-10T14:23:22Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.cub.2019.02.021"],["dc.identifier.issn","0960-9822"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71911"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","A Link between Deoxyribonucleotide Metabolites and Embryonic Cell-Cycle Control"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","856"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","868"],["dc.bibliographiccitation.volume","109"],["dc.contributor.author","Winkler, Franziska"],["dc.contributor.author","Gummalla, Maheshwar"],["dc.contributor.author","Kuenneke, Lutz"],["dc.contributor.author","Lv, Zhiyi"],["dc.contributor.author","Zippelius, Annette"],["dc.contributor.author","Aspelmeier, Timo"],["dc.contributor.author","Grosshans, Joerg"],["dc.date.accessioned","2018-11-07T09:52:29Z"],["dc.date.available","2018-11-07T09:52:29Z"],["dc.date.issued","2015"],["dc.description.abstract","The actin and microtubule networks form the dynamic cytoskeleton. Network dynamics is driven by molecular motors applying force onto the networks and the interactions between the networks. Here we assay the dynamics of centrosomes in the scale of seconds as a proxy for the movement of microtubule asters. With this assay we want to detect the role of specific motors and of network interaction. During interphase of syncytial embryos of Drosophila, cortical actin and the microtubule network depend on each other. Centrosomes induce cortical actin to form caps, whereas F-actin anchors microtubules to the cortex. In addition, lateral interactions between microtubule asters are assumed to be important for regular spatial organization of the syncytial embryo. The functional interaction between the microtubule asters and cortical actin has been largely analyzed in a static manner, so far. We recorded the movement of centrosomes at 1 Hz and analyzed their fluctuations for two processes-pair separation and individual movement. We found that F-actin is required for directional movements during initial centrosome pair separation, because separation proceeds in a diffusive manner in latrunculin-injected embryos. For assaying individual movement, we established a fluctuation parameter as the deviation from temporally and spatially slowly varying drift movements. By analysis of mutant and drug-injected embryos, we found that the fluctuations were suppressed by both cortical actin and microtubules. Surprisingly, the microtubule motor Kinesin-1 also suppressed fluctuations to a similar degree as F-actin. Kinesin-1 may mediate linkage of the microtubule (l)-ends to the actin cortex. Consistent with this model is our finding that Kinesin-1-GFP accumulates at the cortical actin caps."],["dc.identifier.doi","10.1016/j.bpj.2015.07.044"],["dc.identifier.isi","000360960500004"],["dc.identifier.pmid","26331244"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36133"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Fluctuation Analysis of Centrosomes Reveals a Cortical Function of Kinesin-1"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2305"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","G3 (Bethesda, Md.)"],["dc.bibliographiccitation.lastpage","2314"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Winkler, Franziska"],["dc.contributor.author","Kriebel, Maria"],["dc.contributor.author","Clever, Michaela"],["dc.contributor.author","Gröning, Stephanie"],["dc.contributor.author","Großhans, Jörg"],["dc.date.accessioned","2019-07-09T11:43:38Z"],["dc.date.available","2019-07-09T11:43:38Z"],["dc.date.issued","2017-07-05"],["dc.description.abstract","Many metabolic enzymes are evolutionarily highly conserved and serve a central function in the catabolism and anabolism of cells. The serine hydroxymethyl transferase (SHMT) catalyzing the conversion of serine and glycine and vice versa feeds into tetrahydrofolate (THF)-mediated C1 metabolism. We identified a Drosophila mutation in SHMT (CG3011) in a screen for blastoderm mutants. Embryos from SHMT mutant germline clones specifically arrest the cell cycle in interphase 13 at the time of the midblastula transition (MBT) and prior to cellularization. The phenotype is due to a loss of enzymatic activity as it cannot be rescued by an allele with a point mutation in the catalytic center but by an allele based on the SHMT coding sequence from Escherichia coli The onset of zygotic gene expression and degradation of maternal RNAs in SHMT mutant embryos are largely similar to that in wild-type embryos. The specific timing of the defects in SHMT mutants indicates that at least one of the SHMT-dependent metabolites becomes limiting in interphase 13, if it is not produced by the embryo. Our data suggest that mutant eggs contain maternally-provided and SHMT-dependent metabolites in amounts that suffice for early development until interphase 13."],["dc.identifier.doi","10.1534/g3.117.043133"],["dc.identifier.pmid","28515048"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14608"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58933"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2160-1836"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Essential Function of the Serine Hydroxymethyl Transferase (SHMT) Gene During Rapid Syncytial Cell Cycles in Drosophila."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]