Griesinger, Christian

[["dc.bibliographiccitation.firstpage","S177"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Magnetic Resonance in Chemistry"],["dc.bibliographiccitation.lastpage","S186"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Zimmer, D. P."],["dc.contributor.author","Marino, J. P."],["dc.contributor.author","Griesinger, C."],["dc.date.accessioned","2018-04-23T11:47:41Z"],["dc.date.available","2018-04-23T11:47:41Z"],["dc.date.issued","1996"],["dc.description.abstract","A set of experiments to determine homo- and heteronuclear vicinal coupling constants in uniformly C-13-labeled DNA oligonucleotides is presented, 2D and 3D HCCH-E.COSY experiments were used to measure 3J(HH) coupling constants in the deoxyribose ring, a refocused HMBC experiment was used to measure (3)J(CH) coupling constants about the glycosidic torsion angle chi and a P-FIDS-CT-HSQC experiment was used to determine 2J(CP) and 3J(HP) coupling constants about the backbone torsion angle epsilon. The experiments were demonstrated on a uniformly C-13,N-15-labeled 10 base pair (bp) DNA duplex which contains a dA . dT tract 4 bp in length. With these experiments it was possible to extract a number of the coupling constants from duplex DNA."],["dc.identifier.doi","10.1002/(SICI)1097-458X(199612)34:133.0.CO;2-S"],["dc.identifier.gro","3144629"],["dc.identifier.isi","A1996WK90900021"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2274"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0749-1581"],["dc.relation.issn","0749-1581"],["dc.title","Determination of Homo- and Heteronuclear Coupling Constants in Uniformly13C,15N-Labeled DNA Oligonucleotides"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","160"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Magnetic Resonance, Series B"],["dc.bibliographiccitation.lastpage","180"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Glaser, S. J."],["dc.contributor.author","Schwalbe, Harald"],["dc.contributor.author","Marino, J. P."],["dc.contributor.author","Griesinger, Christian"],["dc.date.accessioned","2017-09-07T11:51:08Z"],["dc.date.available","2017-09-07T11:51:08Z"],["dc.date.issued","1996"],["dc.description.abstract","The directed TOCSY pulse sequence element transfers coherence predominantly into 'forward-directed' antiphase coherences while simultaneously suppressing in-phase and 'backward-directed' antiphase coherences. This novel selection principle, based on the 'direction' of the target coherences, provides a new approach for the simplification of crowded spectra. In this article, the theory of directed TOCSY is presented for linear spin systems that are frequently found in carbon-labeled biomolecules. (C) 1996 Academic Press, Inc."],["dc.identifier.doi","10.1006/jmrb.1996.0126"],["dc.identifier.gro","3144642"],["dc.identifier.isi","A1996VB16300008"],["dc.identifier.pmid","8812900"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2288"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Academic Press Inc Jnl-comp Subscriptions"],["dc.relation.issn","1064-1866"],["dc.title","Directed TOCSY, a method for selection of directed correlations by optimal combinations of isotropic and longitudinal mixing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7251"],["dc.bibliographiccitation.issue","27"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.bibliographiccitation.lastpage","7252"],["dc.bibliographiccitation.volume","117"],["dc.contributor.author","Schwalbe, Harald"],["dc.contributor.author","Marino, J. P."],["dc.contributor.author","Glaser, S. J."],["dc.contributor.author","Griesinger, Christian"],["dc.date.accessioned","2017-09-07T11:51:11Z"],["dc.date.available","2017-09-07T11:51:11Z"],["dc.date.issued","1995"],["dc.identifier.doi","10.1021/ja00132a028"],["dc.identifier.gro","3144682"],["dc.identifier.isi","A1995RJ03500028"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2332"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0002-7863"],["dc.title","MEASUREMENT OF H,H-COUPLING CONSTANTS ASSOCIATED WITH NU-1, NU-2, AND NU-3 IN UNIFORMLY C-13-LABELED RNA BY HCC-TOCSY-CCH-E.COSY"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","631"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Biomolecular NMR"],["dc.bibliographiccitation.lastpage","644"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Schwalbe, Harald"],["dc.contributor.author","Marino, J. P."],["dc.contributor.author","KING, GC"],["dc.contributor.author","WECHSELBERGER, R. W."],["dc.contributor.author","Bermel, W."],["dc.contributor.author","Griesinger, Christian"],["dc.date.accessioned","2017-09-07T11:51:25Z"],["dc.date.available","2017-09-07T11:51:25Z"],["dc.date.issued","1994"],["dc.description.abstract","Three experiments are introduced to determine a complete set of coupling constants in RNA oligomers. In the HCCH-E.COSY experiment, the vicinal proton-proton coupling constants can be measured with high accuracy. In the P-FIDS-CT-HSQC experiment, vicinal proton-phosphorus and carbon-phosphorus couplings are measured that depend on the phosphodiester backbone torsion angles beta and epsilon. In the refocussed HMBC experiment, vicinal carbon-proton couplings are measured that depend on the glycosidic torsion angle chi."],["dc.identifier.doi","10.1007/BF00404274"],["dc.identifier.gro","3144723"],["dc.identifier.isi","A1994PE35200004"],["dc.identifier.pmid","7919950"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2379"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: NIGMS NIH HHS [GM 21966]"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Escom Sci Publ Bv"],["dc.relation.issn","0925-2738"],["dc.title","DETERMINATION OF A COMPLETE SET OF COUPLING-CONSTANTS IN C-13-LABELED OLIGONUCLEOTIDES"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4388"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.bibliographiccitation.lastpage","4395"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Marino, J. P."],["dc.contributor.author","Schwalbe, Harald"],["dc.contributor.author","Glaser, S. J."],["dc.contributor.author","Griesinger, Christian"],["dc.date.accessioned","2017-09-07T11:51:08Z"],["dc.date.available","2017-09-07T11:51:08Z"],["dc.date.issued","1996"],["dc.description.abstract","The conformational analysis of the backbone angle gamma (O5'-C5'-C4'-C3') and the stereospecific assignment of the H5'((pro-S)), H5'((pro-R)) diastereotopic protons in a uniformly C-13,N-15 labeled RNA oligonucleotide was performed using new Exclusive COSY (E.COSY) type multidimensional heteronuclear NMR experiments designed to measure (3)J(H4',H5') homonuclear and (2)J(C4',H5' heteronuclear coupling constants. The experiments were demonstrated on a uniformly C-13,N-15 labeled 19-mer RNA hairpin (5'rGCACCGUUGGUAGCGGUGC-3') derived from the RNA I transcript involved in Col El replication control. From the small (3)J(H4',H5') couplings constants observed for the RNA hairpin, it was concluded that all gamma angles assume a gauche(+) rotamer conformation (gamma = 60 degrees). From the signs of the (2)J(C4',H5') coupling constants, the H5' protons were stereospecifically assigned. In the helical stem region of the hairpin, the H5'((pro-S)) protons were found to resonate downfield (similar to 0.4 ppm) of the H5'((pro-R)) protons. In the loop region of the hairpin, the chemical shift differences between the H5'((pro-S)), H5'((pro-R)) resonances were found to be smaller, and in most cases, the H5'((pro-S)) protons were found to resonate upfield of the H5'((pro-R)) protons. The different chemical shift patterns observed for the H5'((pro-R)) and H5'((pro-R)) protons in the two secondary structure elements are discussed."],["dc.identifier.doi","10.1021/ja953554o"],["dc.identifier.gro","3144650"],["dc.identifier.isi","A1996UK14500020"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2297"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0002-7863"],["dc.title","Determination of gamma and stereospecific assignment of H5' protons by measurement of (2)J and (3)J coupling constants in uniformly C-13 labeled RNA"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","223"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Biomolecular NMR"],["dc.bibliographiccitation.lastpage","230"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Richter, C."],["dc.contributor.author","Reif, Bernd"],["dc.contributor.author","Worner, K"],["dc.contributor.author","Quant, S."],["dc.contributor.author","Marino, J. P."],["dc.contributor.author","Engels, J. W."],["dc.contributor.author","Griesinger, Christian"],["dc.contributor.author","Schwalbe, Harald"],["dc.date.accessioned","2017-09-07T11:48:08Z"],["dc.date.available","2017-09-07T11:48:08Z"],["dc.date.issued","1998"],["dc.description.abstract","A new experiment for the measurement of (n)J(C,P) coupling constants along the phosphodiester backbone in RNA and DNA based on a quantitative-J HCP experiment is presented. In addition to coupling constants, in which a carbon atom couples to only one phosphorus atom, both the intraresidual (3)J(C4(i)',Pi) and the sequential (3)J(C4(i)',Pi+1) for the C4' resonances that couple to two phosphorus atoms can be obtained. Coupling constants obtained by this new method are compared to values obtained from the P-FIDS experiment. Together with (3)J(H,P) coupling constants measured using the P-FIDS experiment, the backbone angles beta and epsilon can be determined."],["dc.identifier.doi","10.1023/A:1008258226683"],["dc.identifier.gro","3144529"],["dc.identifier.isi","000075844500002"],["dc.identifier.pmid","21136326"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2163"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Kluwer Academic Publ"],["dc.relation.issn","0925-2738"],["dc.title","A new experiment for the measurement of (n)J(C,P) coupling constants including (3)J(C4(i)('),P-i) and (3)J(C4(i)('),Pi+1) in oligonucleotides"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","259"],["dc.bibliographiccitation.lastpage","367"],["dc.bibliographiccitation.seriesnr","16"],["dc.contributor.author","Griesinger, Christian"],["dc.contributor.author","Hennig, Matthias H."],["dc.contributor.author","Marino, J. P."],["dc.contributor.author","Reft, B."],["dc.contributor.author","Richter, C."],["dc.contributor.author","Schwalbe, Harald"],["dc.contributor.editor","Krishna, N. Rama"],["dc.contributor.editor","Berliner, Lawrence J."],["dc.date.accessioned","2017-09-07T11:52:27Z"],["dc.date.available","2017-09-07T11:52:27Z"],["dc.date.issued","1998"],["dc.identifier.gro","3144927"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2605"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","Kluwer Academic"],["dc.publisher.place","New York"],["dc.relation.crisseries","Biological Magnetic Resonance"],["dc.relation.isbn","0-306-45952-3"],["dc.relation.ispartof","Modern Techniques in Protein NMR"],["dc.relation.ispartofseries","Biological Magnetic Resonance; 16"],["dc.title","Methods for the Determination of Torsion Angle Restraints in Biomacromolecules"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7361"],["dc.bibliographiccitation.issue","31"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.bibliographiccitation.lastpage","7366"],["dc.bibliographiccitation.volume","119"],["dc.contributor.author","Marino, J. P."],["dc.contributor.author","Diener, J. L."],["dc.contributor.author","Moore, P. B."],["dc.contributor.author","Griesinger, Christian"],["dc.date.accessioned","2017-09-07T11:50:55Z"],["dc.date.available","2017-09-07T11:50:55Z"],["dc.date.issued","1997"],["dc.description.abstract","One-bond H-1, C-13 and geminal H-1,H-1 dipolar interactions are normally the dominant causes of H-1 and C-13 transverse relaxation in NMR experiments applied to C-13,N-15-labeled RNA in solution. Proton,carbon multiple-quantum coherences, where all heteronuclei connected by single bonds are evolved simultaneously in the transverse plane, however, are not affected by these strong dipolar interactions. Consequently, the transverse lifetimes, or T-2 values, of these resonances can be dramatically extended. Here, we show that pulse sequences can be written that take advantage of this effect to enhance the sensitivity with which CH and CH2 correlations are observed in uniformly C-13,N-15-labeled RNA oligonucleotides. In heteronuclear multiple-quantum correlation experiments, CH and CH2 correlations are detected with a sensitivity that is enhanced by about a factor of 3 relative to heteronuclear, single-quantum experiments for a C-13,N-15-labeled 36mer RNA oligonucleotide and a constant time period of 25 ms. By including H-1, C-13 multiple-quantum coherence steps in an H-1, C-13, N-15 NMR experiment of the 'out and back' type currently used for through-bond resonance assignment in RNA, we have obtained a sensitivity enhancement of about a factor of 5 in the same 36mer RNA."],["dc.identifier.doi","10.1021/ja964379u"],["dc.identifier.gro","3144594"],["dc.identifier.isi","A1997XP87700023"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2236"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0002-7863"],["dc.title","Multiple-quantum coherence dramatically enhances the sensitivity of CH and CH2 correlations in uniformly C-13-labeled RNA"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","614"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Accounts of Chemical Research"],["dc.bibliographiccitation.lastpage","623"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Marino, J. P."],["dc.contributor.author","Schwalbe, Harald"],["dc.contributor.author","Griesinger, Christian"],["dc.date.accessioned","2017-09-07T11:47:29Z"],["dc.date.available","2017-09-07T11:47:29Z"],["dc.date.issued","1999"],["dc.identifier.doi","10.1021/ar9600392"],["dc.identifier.gro","3144458"],["dc.identifier.isi","000081680800009"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2084"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0001-4842"],["dc.title","J-coupling restraints in RNA structure determination"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]