Burckhardt, Birgitta-Christina

[["dc.bibliographiccitation.firstpage","447"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Croatian medical journal"],["dc.bibliographiccitation.lastpage","459"],["dc.bibliographiccitation.volume","56"],["dc.contributor.author","Breljak, Davorka"],["dc.contributor.author","Brzica, Hrvoje"],["dc.contributor.author","Vrhovac, Ivana"],["dc.contributor.author","Micek, Vedran"],["dc.contributor.author","Karaica, Dean"],["dc.contributor.author","Ljubojević, Marija"],["dc.contributor.author","Sekovanić, Ankica"],["dc.contributor.author","Jurasović, Jasna"],["dc.contributor.author","Rašić, Dubravka"],["dc.contributor.author","Peraica, Maja"],["dc.contributor.author","Lovrić, Mila"],["dc.contributor.author","Schnedler, Nina"],["dc.contributor.author","Henjakovic, Maja"],["dc.contributor.author","Wegner, Waja"],["dc.contributor.author","Burckhardt, Gerhard"],["dc.contributor.author","Burckhardt, Birgitta C."],["dc.contributor.author","Sabolić, Ivan˝"],["dc.date.accessioned","2019-07-10T08:11:57Z"],["dc.date.available","2019-07-10T08:11:57Z"],["dc.date.issued","2015-10-31"],["dc.description.abstract","Aim To investigate whether the sex-dependent expression of hepatic and renal oxalate transporter sat-1 (Slc26a1) changes in a rat model of ethylene glycol (EG)-induced hyperoxaluria. METHODS: Rats were given tap water (12 males and 12 females; controls) or EG (12 males and 12 females; 0.75% v/v in tap water) for one month. Oxaluric state was confirmed by biochemical parameters in blood plasma, urine, and tissues. Expression of sat-1 and rate-limiting enzymes of oxalate synthesis, alcohol dehydrogenase 1 (Adh1) and hydroxy-acid oxidase 1 (Hao1), was determined by immunocytochemistry (protein) and/or real time reverse transcription polymerase chain reaction (mRNA). RESULTS: EG-treated males had significantly higher (in μmol/L; mean±standard deviation) plasma (59.7±27.2 vs 12.9±4.1, P<0.001) and urine (3716±1726 vs 241±204, P<0.001) oxalate levels, and more abundant oxalate crystaluria than controls, while the liver and kidney sat-1 protein and mRNA expression did not differ significantly between these groups. EG-treated females, in comparison with controls had significantly higher (in μmol/L) serum oxalate levels (18.8±2.9 vs 11.6±4.9, P<0.001), unchanged urine oxalate levels, low oxalate crystaluria, and significantly higher expression (in relative fluorescence units) of the liver (1.59±0.61 vs 0.56±0.39, P=0.006) and kidney (1.77±0.42 vs 0.69±0.27, P<0.001) sat-1 protein, but not mRNA. The mRNA expression of Adh1 was female-dominant and that of Hao1 male-dominant, but both were unaffected by EG treatment. CONCLUSIONS: An increased expression of hepatic and renal oxalate transporting protein sat-1 in EG-treated female rats could protect from hyperoxaluria and oxalate urolithiasis."],["dc.identifier.pmid","26526882"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12681"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60827"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1332-8166"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.title","In female rats, ethylene glycol treatment elevates protein expression of hepatic and renal oxalate transporter sat-1 (Slc26a1) without inducing hyperoxaluria."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1381"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Pflügers Archiv - European Journal of Physiology"],["dc.bibliographiccitation.lastpage","1392"],["dc.bibliographiccitation.volume","457"],["dc.contributor.author","Brzica, Hrvoje"],["dc.contributor.author","Breljak, Davorka"],["dc.contributor.author","Krick, Wolfgang"],["dc.contributor.author","Lovric, Mila"],["dc.contributor.author","Burckhardt, Gerhard"],["dc.contributor.author","Burckhardt, Birgitta-Christina"],["dc.contributor.author","Sabolic, Ivan"],["dc.date.accessioned","2018-11-07T08:31:22Z"],["dc.date.available","2018-11-07T08:31:22Z"],["dc.date.issued","2009"],["dc.description.abstract","The sulfate anion transporter (sat-1, Slc26a1) has been cloned from rat liver, functionally characterized, and localized to the sinusoidal membrane in hepatocytes and basolateral membrane (BLM) in proximal tubules (PT). Here, we confirm previously described localization of sat-1 protein in rat liver and kidneys and report on gender differences (GD) in its expression by immunochemical, transport, and excretion studies in rats. The similar to 85-kDa sat-1 protein was localized to the sinusoidal membrane in hepatocytes and BLM in renal cortical PT, with the male-dominant expression. However, the real-time reverse-transcription polymerase chain reaction data indicated no GD at the level of sat-1 mRNA. In agreement with the protein data, isolated membranes from both organs exhibited the male-dominant exchange of radiolabeled sulfate for oxalate, whereas higher oxalate in plasma and 24-h urine indicated higher oxalate production and excretion in male rats. Furthermore, the expression of liver, but not renal, sat-1 protein was: unaffected by castration, upregulated by ovariectomy, and downregulated by estrogen or progesterone treatment in males. Therefore, GD (males > females) in the expression of sat-1 protein in rat liver (and, possibly, kidneys) are caused by the female sex-hormone-driven inhibition at the posttranscriptional level. The male-dominant abundance of sat-1 protein in liver may conform to elevated uptake of sulfate and extrusion of oxalate, causing higher plasma oxalate in males. Oxalate is then excreted by the kidneys via the basolateral sat-1 (males > females) and the apical CFEX (Slc26a6; GD unknown) in PT and eliminated in the urine (males > females), where it may contribute to the male-prevailing development of oxalate urolithiasis."],["dc.identifier.doi","10.1007/s00424-008-0611-5"],["dc.identifier.isi","000264184300015"],["dc.identifier.pmid","19002488"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3531"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17108"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0031-6768"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","The liver and kidney expression of sulfate anion transporter sat-1 in rats exhibits male-dominant gender differences"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","16332"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Journal of Biological Chemistry"],["dc.bibliographiccitation.lastpage","16341"],["dc.bibliographiccitation.volume","283"],["dc.contributor.author","Bahn, Andrew"],["dc.contributor.author","Hagos, Yohannes"],["dc.contributor.author","Reuter, Stefan"],["dc.contributor.author","Balen, Daniela"],["dc.contributor.author","Brzica, Hrvoje"],["dc.contributor.author","Krick, Wolfgang"],["dc.contributor.author","Burckhardt, Birgitta C."],["dc.contributor.author","Sabolić, Ivan"],["dc.contributor.author","Burckhardt, Gerhard"],["dc.date.accessioned","2021-06-01T10:51:08Z"],["dc.date.available","2021-06-01T10:51:08Z"],["dc.date.issued","2008"],["dc.identifier.doi","10.1074/jbc.M800737200"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6066"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86903"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0021-9258"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Identification of a New Urate and High Affinity Nicotinate Transporter, hOAT10 (SLC22A13)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]