Chmyrov, Andriy

[["dc.bibliographiccitation.firstpage","655"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","ChemPhysChem"],["dc.bibliographiccitation.lastpage","663"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Lavoie-Cardinal, Flavie"],["dc.contributor.author","Jensen, Nickels A."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Chmyrov, Andriy"],["dc.contributor.author","Bierwagen, Jakob"],["dc.contributor.author","Testa, Ilaria"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:46:25Z"],["dc.date.available","2017-09-07T11:46:25Z"],["dc.date.issued","2014"],["dc.description.abstract","Up to now, all demonstrations of reversible saturable optical fluorescence transitions (RESOLFT) superresolution microscopy of living cells have relied on the use of reversibly switchable fluorescent proteins (RSFP) emitting in the green spectral range. Here we show RESOLFT imaging with rsCherryRev1.4, a new red-emitting RSFP enabling a spatial resolution up to four times higher than the diffraction barrier. By co-expressing green and red RSFPs in living cells we demonstrate two-color RESOLFT imaging both for single (donut) beam scanning and for parallelized versions of RESOLFT nanoscopy where an array of >23000 donut-like minima are scanned simultaneously."],["dc.identifier.doi","10.1002/cphc.201301016"],["dc.identifier.gro","3142168"],["dc.identifier.isi","000332747500013"],["dc.identifier.pmid","24449030"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12826"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5288"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1439-7641"],["dc.relation.issn","1439-4235"],["dc.rights","CC BY-NC-ND 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/3.0"],["dc.title","Two-Color RESOLFT Nanoscopy with Green and Red Fluorescent Photochromic Proteins"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","44619"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.lastpage","9"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Chmyrov, Andriy"],["dc.contributor.author","Leuschner, Ivo"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Keller-Findeisen, Jan"],["dc.contributor.author","Kastrup, Lars"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Donnert, Gerald"],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:50:39Z"],["dc.date.available","2017-09-07T11:50:39Z"],["dc.date.issued","2017"],["dc.description.abstract","Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a few seconds. This advance relies on using light patterns featuring substantial arrays of intensity minima for discerning features by switching their fluorophores between ‘on’ and ‘off’ states of fluorescence. Here we show that splitting the light with a grating and recombining it in the focal plane of the objective lens renders arrays of minima with wavelength-independent periodicity. This colour-independent creation of periodic patterns facilitates coaligned on- and off-switching and readout with combinations chosen from a range of wavelengths. Applying up to three such periodic patterns on the switchable fluorescent proteins Dreiklang and rsCherryRev1.4, we demonstrate highly parallelized, multicolour RESOLFT nanoscopy in living cells for ~100 × 100 μm2 fields of view. Individual keratin filaments were rendered at a FWHM of ~60–80 nm, with effective resolution for the filaments of ~80–100 nm. We discuss the impact of novel image reconstruction algorithms featuring background elimination by spatial bandpass filtering, as well as strategies that incorporate complete image formation models."],["dc.identifier.doi","10.1038/srep44619"],["dc.identifier.gro","3145910"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14935"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3644"],["dc.language.iso","en"],["dc.notes.intern","lifescience"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","chake"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Achromatic light patterning and improved image reconstruction for parallelized RESOLFT nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]