Schlumbohm, Christina

[["dc.bibliographiccitation.firstpage","155"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of Medical Primatology"],["dc.bibliographiccitation.lastpage","164"],["dc.bibliographiccitation.volume","35"],["dc.contributor.author","Atanasova, Srebrena"],["dc.contributor.author","von Ahsen, Nicolas"],["dc.contributor.author","Schlumbohm, Christina"],["dc.contributor.author","Wieland, Eberhard"],["dc.contributor.author","Oellerich, M."],["dc.contributor.author","Armstrong, Victor William"],["dc.date.accessioned","2018-11-07T09:45:57Z"],["dc.date.available","2018-11-07T09:45:57Z"],["dc.date.issued","2006"],["dc.description.abstract","Background Dysfunction of the cellular antioxidant system and accumulation of reactive oxygen species are involved in the pathophysiology of diseases such as cardiovascular disease, neurodegenerative disorders, tumors, male infertility and aging. Two gluthathione peroxidases play key roles in the cellular protection against oxidative damage. Glutathione peroxidase (GPx-1) removes cytosolic hydroperoxides while phospholipid-hydroperoxide glutathione peroxidase (GPx-4) is a unique enzyme that reduces phospholipid peroxides in membranes. Methods We cloned and sequenced the full-length cDNA for GPx-1 (GenBank: AY966403) and GPx-4 (GenBank: AY966404) from the common marmoset (Callithrix jacchus) in order to create a suitable model for studying human diseases related with oxidative stress. Results The cDNAs encode a 202 amino acid protein for GPx-1 and a 197 amino acid protein for GPx-4. Both proteins include selenocysteine (Sec, in Gpx-1 at position 48; in GPx-4 at position 73) and showed high homology (> 90%) with other mammalian GPxs. The relative levels of mRNA expression for GPx-1 and GPx-4 were determined in different marmoset tissues by quantitative real-time reverse transcriptase-polymerase chain reaction using transcription elongation factor-2 as a reference gene. GPx-1 showed increased levels of expression in the liver, heart and kidney while the highest mRNA levels for GPx-4 were detected in the testis, followed by the liver, lung, kidney and spinal cord. Conclusions These findings will be of value for studies designed to assess the role of glutathione peroxidases in non-human primate models for a variety of diseases in which increased oxidative stress has been implicated."],["dc.identifier.doi","10.1111/j.1600-0684.2006.00158.x"],["dc.identifier.isi","000237352700007"],["dc.identifier.pmid","16764674"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34757"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing"],["dc.relation.issn","0047-2565"],["dc.title","Marmoset glutathione peroxidases: cDNA sequences, molecular evolution, and gene expression"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2873"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Diabetes"],["dc.bibliographiccitation.lastpage","2879"],["dc.bibliographiccitation.volume","58"],["dc.contributor.author","Nyirenda, Moffat J."],["dc.contributor.author","Carter, Roderick"],["dc.contributor.author","Tang, Justin I."],["dc.contributor.author","de Vries, Annick"],["dc.contributor.author","Schlumbohm, Christina"],["dc.contributor.author","Hillier, Stephen G."],["dc.contributor.author","Streit, Frank"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Armstrong, Victor William"],["dc.contributor.author","Fuchs, Eberhard"],["dc.contributor.author","Seckl, Jonathan R."],["dc.date.accessioned","2018-11-07T11:21:24Z"],["dc.date.available","2018-11-07T11:21:24Z"],["dc.date.issued","2009"],["dc.description.abstract","OBJECTIVE-Recent studies in humans and animal models of obesity have shown increased adipose tissue activity of 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1), which amplifies local I issue glucocorticoid concentrations. The reasons for this 11 beta-HSD1 dystegulation are unknown. Here, we tested whether 11 beta-HSD1 expression, like the metabolic syndrome, is \"programmed\" by prenatal environmental events in a nonhuman primate model, the common marmoset monkey. RESEARCH DESIGN AND METHODS-We used a \"fetal programming\" paradigm where brief antenatal exposure to glucocorticoids leads to the metabolic syndrome in the offspring. Pregnant marmosets were given the synthetic glucocorticoid dexamethasone orally for 1 week in either early or late gestation, or they were given vehicle. Tissue 11 beta-HSD1 and glucocorticoid receptor mRNA expression were examined in the offspring at 4 and 24 months of age. RESULTS-Prenatal dexamethasone administration, selectively during late gestation, resulted in early and persistent elevations in 11 beta-HSD1 mRNA expression and activity in the liver, pancreas, and subcutaneous-but not visceral-fat. The increase in 11 beta-HSD1 occurred before animals developed obesity or overt features of the metabolic syndrome. In contrast to rodents, in utero dexamethasone exposure did not alter glucocorticoid receptor expression in metabolic tissues in marmosets. CONCLUSIONS-These data suggest that long-term upregulation of 11 beta-HSD1 in metabolically active tissues may follow prenatal \"stress\" hormone exposure and indicates a novel mechanism for fetal origins of adult obesity and the metabolic syndrome. Diabetes 58:2873-2879, 2009"],["dc.description.sponsorship","European Commission [QLRT-2001-02758]; MRC"],["dc.identifier.doi","10.2337/db09-0873"],["dc.identifier.isi","000272522000021"],["dc.identifier.pmid","19720800"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55764"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Diabetes Assoc"],["dc.relation.issn","0012-1797"],["dc.title","Prenatal Programming of Metabolic Syndrome in the Common Marmoset Is Associated With Increased Expression of 11 beta-Hydroxysteroid Dehydrogenase Type 1"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Experimental and Clinical Endocrinology & Diabetes"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Schlumbohm, Christina"],["dc.contributor.author","Bramlage, Carsten Paul"],["dc.contributor.author","Strutz, Frank M."],["dc.contributor.author","Armstrong, Victor William"],["dc.contributor.author","Oellerich, M."],["dc.contributor.author","Fuchs, E."],["dc.date.accessioned","2018-11-07T10:58:58Z"],["dc.date.available","2018-11-07T10:58:58Z"],["dc.date.issued","2007"],["dc.format.extent","548"],["dc.identifier.isi","000250144000040"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50589"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Johann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh"],["dc.publisher.place","Stuttgart"],["dc.relation.issn","0947-7349"],["dc.title","Predictive value of maternal bodyweight, postnatal weight gain and prenatal clexamethasone overexposure for the development of obesity in adult marmoset monkeys"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","190"],["dc.bibliographiccitation.journal","BMC genomics"],["dc.bibliographiccitation.lastpage","9"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Datson, Nicole A."],["dc.contributor.author","Morsink, Maarten C."],["dc.contributor.author","Atanasova, Srebrena"],["dc.contributor.author","Armstrong, Victor W."],["dc.contributor.author","Zischler, Hans"],["dc.contributor.author","Schlumbohm, Christina"],["dc.contributor.author","Dutilh, Bas E."],["dc.contributor.author","Huynen, Martijn A."],["dc.contributor.author","Waegele, Brigitte"],["dc.contributor.author","Ruepp, Andreas"],["dc.contributor.author","Kloet, E. Ronald"],["dc.contributor.author","Fuchs, Eberhard"],["dc.date.accessioned","2019-07-10T08:13:00Z"],["dc.date.available","2019-07-10T08:13:00Z"],["dc.date.issued","2007"],["dc.description.abstract","Background: The common marmoset monkey (Callithrix jacchus), a small non-endangered New World primate native to eastern Brazil, is becoming increasingly used as a non-human primate model in biomedical research, drug development and safety assessment. In contrast to the growing interest for the marmoset as an animal model, the molecular tools for genetic analysis are extremely limited.Results: Here we report the development of the first marmoset-specific oligonucleotide microarray (EUMAMA) containing probe sets targeting 1541 different marmoset transcripts expressed in hippocampus. These 1541 transcripts represent a wide variety of different functional gene classes. Hybridisation of the marmoset microarray with labelled RNA from hippocampus, cortex and a panel of 7 different peripheral tissues resulted in high detection rates of 85% in the neuronal tissues and on average 70% in the non-neuronal tissues. The expression profiles of the 2 neuronal tissues, hippocampus and cortex, were highly similar, as indicated by a correlation coefficient of 0.96. Several transcripts with a tissue-specific pattern of expression were identified. Besides the marmoset microarray we have generated 3215 ESTs derived from marmoset hippocampus, which have been annotated and submitted to GenBank [GenBank: EF214838 EF215447, EH380242 EH382846]. Conclusion: We have generated the first marmoset-specific DNA microarray and demonstrated its use to characterise large-scale gene expression profiles of hippocampus but also of other neuronal and non-neuronal tissues. In addition, we have generated a large collection of ESTs of marmoset origin, which are now available in the public domain. These new tools will facilitate molecular genetic research into this non-human primate animal model."],["dc.identifier.fs","91281"],["dc.identifier.ppn","560256167"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/4367"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61097"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Development of the first marmoset-specific DNA microarray (EUMAMA): a new genetic tool for large-scale expression profiling in a non-human primate"],["dc.title.alternative","Research article"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","PII 905441944"],["dc.bibliographiccitation.firstpage","215"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Stress"],["dc.bibliographiccitation.lastpage","224"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Atanasova, Srebrena"],["dc.contributor.author","Wieland, Eberhard"],["dc.contributor.author","Schlumbohm, Christina"],["dc.contributor.author","Korecka, M."],["dc.contributor.author","Shaw, Leslie M."],["dc.contributor.author","von Ahsen, Nicolas"],["dc.contributor.author","Fuchs, E."],["dc.contributor.author","Oellerich, M."],["dc.contributor.author","Armstrong, Victor William"],["dc.date.accessioned","2018-11-07T08:35:33Z"],["dc.date.available","2018-11-07T08:35:33Z"],["dc.date.issued","2009"],["dc.description.abstract","Human epidemiological studies have indicated that low birth weight associated with an adverse intrauterine environment is related to a greater incidence of cardiovascular disorders in later life. In the foetus, endogenous glucocorticoids generally increase if there is intrauterine nutrient deficiency. The consequent glucocorticoid hyperexposure has been hypothesised to cause in utero programming of atherogenic genes. We investigated the effect of oral treatment with the synthetic glucocorticoid dexamethasone during early or late pregnancy in marmoset monkeys on oxidative and antioxidant status in the offspring. Urinary concentrations of F2-isoprostanes were quantified as markers for in vivo oxidative stress. Expression of the mRNAs for the antioxidant enzymes cytosolic glutathione peroxidase (GPx-1), phospholipid hydroperoxide glutathione peroxidase (GPx-4), cytosolic Cu,Zn-superoxide dismutase (SOD1), mitochondrial Mn-superoxide dismutase (SOD2), glutathione reductase (GSR), modifier subunit of glutamate-cysteine ligase (GCLM) and catalase were determined in the aorta. Three groups of pregnant marmosets (10 animals per group) were treated orally for one week with vehicle, or with dexamethasone (5mg/kg daily) during two gestation windows: early dexamethasone group, pregnancy day 42-48, and late dexamethasone group, pregnancy day 90-96. In one male sibling of each litter (10 males per group), aortas were taken at 2 years of age. In the late dexamethasone group a higher aortic mRNA expression for GPx-1 (p0.023), MnSOD (p0.016), GCLM (p0.019) and GSR (p0.014) in comparison to the controls was observed. Aortic expression in the early dexamethasone group was statistically significantly higher only for GSR mRNA (p0.038). No significant changes in urinary F2-isoprostane concentrations between controls, early and late dexamethasone groups at 2 years of age were observed. Hence, prenatal exposure to dexamethasone in the third trimester leads to increased mRNA expression of several aortic antioxidant enzymes in the offspring. This expression pattern was not temporally related to oxidative stress, and it may reflect in utero re-programming of aortic antioxidant gene expression during prenatal glucocorticoid exposure."],["dc.description.sponsorship","European Commission [QLRI-CT-2002-02758, EUPEAH]"],["dc.identifier.doi","10.1080/10253890802305075"],["dc.identifier.isi","000265583600003"],["dc.identifier.pmid","19005875"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18097"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Taylor & Francis Ltd"],["dc.relation.issn","1025-3890"],["dc.title","Prenatal dexamethasone exposure in the common marmoset monkey enhances gene expression of antioxidant enzymes in the aorta of adult offspring"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","39"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Journal of Neuroimmunology"],["dc.bibliographiccitation.lastpage","50"],["dc.bibliographiccitation.volume","176"],["dc.contributor.author","Roelleke, Ulrike"],["dc.contributor.author","Fluegge, Gabriele"],["dc.contributor.author","Plehm, Stephanie"],["dc.contributor.author","Schlumbohm, Christina"],["dc.contributor.author","Armstrong, Victor William"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Uchanska-Ziegler, Barbara"],["dc.contributor.author","Ziegler, Andreas"],["dc.contributor.author","Fuchs, Eberhard"],["dc.contributor.author","Czeh, Boldizsar"],["dc.contributor.author","Walter, Lutz"],["dc.date.accessioned","2018-11-07T09:37:05Z"],["dc.date.available","2018-11-07T09:37:05Z"],["dc.date.issued","2006"],["dc.description.abstract","It has been supposed that central nervous neurons do not express MHC class I molecules. However, recent studies clearly demonstrated functional MHC class I expression in the rodent brain. In the present study, we have extended these studies and investigated the presence of MHC class I transcripts and proteins in the brain of a non-human primate species, the common marmoset monkey (Callithrix jacchus). Using in-situ hybridization, we found strong expression of MHC class I transcripts in neocortex, hippocampal-formation, substantia nigra and nucleus ruber. In-situ hybridization with emulsion autoradiography demonstrated MHC class I mRNA in distinct pyramidal neurons of cortex and hippocampus, in granule neurons of the dentate gyrus, in dopaminergic neurons of substantia nigra and in motor neurons of nucleus ruber. Immunocytochemistry confirmed MHC class I protein expression in these neurons. Two monoclonal. antibodies, MRC-Ox18 and HB115, reacted differentially with MHC class I proteins on neuronal and non-neuronal cells, respectively. Interestingly, in marmoset monkeys that were immumosuppressed with FK506 (tacrolimus), expression of neuronal MHC class I proteins, which could be detected with MRC-Ox18, was either very low (neocortex, nucleus ruber, substantia nigra) or absent (hippocampus). In contrast, class I expression in endothelial cells, which was detected by HB115, was not affected by immunosuppression. Our data show that selected neurons in the brain of a non-human primate express MHC class I molecules and that this expression can be modulated by immunosuppression. (c) 2006 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.jneuroim.2006.04.015"],["dc.identifier.isi","000240323300006"],["dc.identifier.pmid","16750573"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32757"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0165-5728"],["dc.title","Differential expression of major histocompatibility complex class I molecules in the brain of a New World monkey, the common marmoset (Callithrix jacchus)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1210"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Xenobiotica"],["dc.bibliographiccitation.lastpage","1226"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Koehler, S. C."],["dc.contributor.author","von Ahsen, N."],["dc.contributor.author","Schlumbohm, Christina"],["dc.contributor.author","Asif, Abdul Rahman"],["dc.contributor.author","Goedtel-Armbrust, Ute"],["dc.contributor.author","Oellerich, M."],["dc.contributor.author","Wojnowski, Leszek"],["dc.contributor.author","Armstrong, Victor William"],["dc.date.accessioned","2018-11-07T08:56:32Z"],["dc.date.available","2018-11-07T08:56:32Z"],["dc.date.issued","2006"],["dc.description.abstract","Due to its small size and the relative evolutionary proximity, the marmoset has been proposed as a model for studies of human drug interactions and metabolism. The current study investigated the expression and regulation of marmoset CYP3A using mass spectrometry and reporter gene techniques. Expression levels of hepatic marmoset CYP3A protein range from 51 to 123 pmolmg(-1) total protein (mean 85.2 pmolmg(-1), n=10) and CYP3A21 is the dominant hepatic CYP3A protein in marmosets. The sequence similarity between human CYP3A4 and CYP3A21 across the first 7.5 kb of the cloned CYP3A21 promoter is 88% within the xenobiotic-responsive enhancer module (XREM) and the proximal promoter. Both regulatory modules confer transcriptional activation of CYP3A21-luciferase reporter gene constructs cotransfected with hPXR in intestinal LS174T cells. The pronounced response to rifampin and the moderate response to dexamethasone were similar to those observed with CYP3A4. Taken collectively, these data establish substantial similarities in expression and gene regulation between marmoset CYP3A21 and human CYP3A4. CYP3A21 may be an equivalent of CYP3A4 in New World monkeys, consistent with the phylogenetic relationship between these genes. The marmoset, therefore, appears to be a suitable in vivo model to study CYP3A4 function and regulation."],["dc.identifier.doi","10.1080/00498250600962831"],["dc.identifier.isi","000242801400005"],["dc.identifier.pmid","17162468"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23180"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Taylor & Francis Ltd"],["dc.relation.issn","0049-8254"],["dc.title","Marmoset CYP3A21, a model for human CYP3A4: Protein expression and functional characterization of the promoter"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]