Canis, Martin

[["dc.bibliographiccitation.firstpage","468"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Annals of Otology Rhinology & Laryngology"],["dc.bibliographiccitation.lastpage","473"],["dc.bibliographiccitation.volume","122"],["dc.contributor.author","Ihler, Friedrich"],["dc.contributor.author","Sharaf, Kariem"],["dc.contributor.author","Bertlich, Mattis"],["dc.contributor.author","Strieth, Sebastian"],["dc.contributor.author","Reichel, Christoph A."],["dc.contributor.author","Berghaus, Alexander"],["dc.contributor.author","Canis, Martin"],["dc.date.accessioned","2018-11-07T09:22:58Z"],["dc.date.available","2018-11-07T09:22:58Z"],["dc.date.issued","2013"],["dc.description.abstract","Objectives: Tumor necrosis factor alpha (TNF-alpha) is a mediator of inflammation and microcirculation in the cochlea. This study aimed to quantify the effect of a local increase of TNF-alpha and study the effect of its interaction with etanercept on cochlear microcirculation. Methods: Cochlear lateral wall vessels were exposed surgically and assessed by intravital microscopy in guinea pigs in vivo. First, 24 animals were randomly distributed into 4 groups of 6 each. Exposed vessels were superfused repeatedly either with 1 of 3 different concentrations of TNF-alpha (5.0, 0.5, and 0.05 ng/mL) or with placebo (0.9% saline solution). Second, 12 animals were randomly distributed into 2 groups of 6 each. Vessels were pretreated with etanercept (1.0 mu g/mL) or placebo (0.9% saline solution), and then treated by repeated superfusion with TNF-alpha (5.0 ng/mL). Results: TNF-alpha was shown to be effective in decreasing cochlear blood flow at a dose of 5.0 ng/mL (p < 0.01, analysis of variance on ranks). Lower concentrations or placebo treatment did not lead to significant changes. After pretreatment with etanercept, TNF-alpha at a dose of 5.0 ng/mL no longer led to a change in cochlear blood flow. Conclusions: The decreasing effect that TNF-alpha has on cochlear blood flow is dose-dependent. Etanercept abrogates this effect."],["dc.identifier.isi","000321880700011"],["dc.identifier.pmid","23951701"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29467"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Annals Publ Co"],["dc.relation.issn","0003-4894"],["dc.title","Etanercept Prevents Decrease of Cochlear Blood Flow Dose-Dependently Caused by Tumor Necrosis Factor Alpha"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","545"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","International Journal of Audiology"],["dc.bibliographiccitation.lastpage","552"],["dc.bibliographiccitation.volume","52"],["dc.contributor.author","Arpornchayanon, Warangkana"],["dc.contributor.author","Canis, Martin"],["dc.contributor.author","Ihler, Friedrich"],["dc.contributor.author","Settevendemie, Claudia"],["dc.contributor.author","Strieth, Sebastian"],["dc.date.accessioned","2018-11-07T09:22:09Z"],["dc.date.available","2018-11-07T09:22:09Z"],["dc.date.issued","2013"],["dc.description.abstract","Objective: Exposure to loud noise can impair cochlear microcirculation and cause noise-induced hearing loss (NIHL). TNF-alpha signaling has been shown to be activated in NIHL and to control spiral modiolar artery vasoconstriction that regulates cochlear microcirculation. It was the aim of this experimental study to analyse the effects of the TNF-alpha inhibitor etanercept on cochlear microcirculation and hearing threshold shift in NIHL in vivo. Design: After assessment of normacusis using ABR, loud noise (106 dB SPL, 30 minutes) was applied on both ears in guinea pigs. Etanercept was administered systemically after loud noise exposure while control animals received a saline solution. In vivo fluorescence microscopy of strial capillaries was performed after surgical exposure of the cochlea for microcirculatory analysis. ABR measurements were derived from the contralateral ear. Study sample: Guinea pigs (n=6, per group). Results: Compared to controls, cochlear blood flow in strial capillary segments was significantly increased in etanercept-treated animals. Additionally, hearing threshold was preserved in animals receiving the TNF-alpha inhibitor in contrast to a significant threshold raising in controls. Conclusions: TNF-alpha inhibition using etanercept improves cochlear microcirculation and protects hearing levels after loud noise exposure and appears as a promising treatment strategy for human NIHL."],["dc.identifier.doi","10.3109/14992027.2013.790564"],["dc.identifier.isi","000321693900004"],["dc.identifier.pmid","23786392"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29275"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Informa Healthcare"],["dc.relation.issn","1499-2027"],["dc.title","TNF-alpha inhibition using etanercept prevents noise-induced hearing loss by improvement of cochlear blood flow in vivo"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","463"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery"],["dc.bibliographiccitation.lastpage","469"],["dc.bibliographiccitation.volume","145"],["dc.contributor.author","Arpornchayanon, Warangkana"],["dc.contributor.author","Canis, Martin"],["dc.contributor.author","Suckfuell, Markus"],["dc.contributor.author","Ihler, Fritz"],["dc.contributor.author","Olzowy, Bernhard"],["dc.contributor.author","Strieth, Sebastian"],["dc.date.accessioned","2019-07-09T11:53:48Z"],["dc.date.available","2019-07-09T11:53:48Z"],["dc.date.issued","2011-09-01"],["dc.description.abstract","OBJECTIVE: Recent findings support the crucial role of microcirculatory disturbance and ischemia for hearing impairment especially after noise-induced hearing loss (NIHL). The aim of this study was to establish an animal model for in vivo analysis of cochlear microcirculation and hearing function after a loud noise to allow precise measurements of both parameters in vivo. STUDY DESIGN: Randomized controlled trial. Setting. Animal study. Subjects and Methods. After assessment of normacusis (0 minutes) using evoked auditory brainstem responses (ABRs), noise (106-dB sound pressure level [SPL]) was applied to both ears in 6 guinea pigs for 30 minutes while unexposed animals served as controls. In vivo fluorescence microscopy of the stria vascularis capillaries was performed after surgical exposure of 1 cochlea. ABR measurements were derived from the contralateral ear. RESULTS: After noise exposure, red blood cell velocity was reduced significantly by 24.3% (120 minutes) and further decreased to 44.5% at the end of the observation (210 minutes) in contrast to stable control measurements. Vessel diameters were not affected in both groups. A gradual decrease of segmental blood flow became significant (38.1%) after 150 minutes compared with controls. Hearing thresholds shifted significantly from 20.0 ± 5.5 dB SPL (0 minutes) to 32.5 ± 4.2 dB SPL (60 minutes) only in animals exposed to loud noise. CONCLUSION: With regard to novel treatments targeting the stria vascularis in NIHL, this standardized model allows us to analyze in detail cochlear microcirculation and hearing function in vivo."],["dc.identifier.doi","10.1177/0194599811407829"],["dc.identifier.fs","586230"],["dc.identifier.pmid","21636842"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8048"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60497"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1097-6817"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Auditory Threshold"],["dc.subject.mesh","Cochlea"],["dc.subject.mesh","Disease Models, Animal"],["dc.subject.mesh","Evoked Potentials, Auditory, Brain Stem"],["dc.subject.mesh","Guinea Pigs"],["dc.subject.mesh","Hearing Loss, Noise-Induced"],["dc.subject.mesh","Loudness Perception"],["dc.subject.mesh","Male"],["dc.subject.mesh","Microcirculation"],["dc.subject.mesh","Microscopy, Fluorescence"],["dc.subject.mesh","Noise"],["dc.subject.mesh","Random Allocation"],["dc.subject.mesh","Statistics, Nonparametric"],["dc.title","Modeling the measurements of cochlear microcirculation and hearing function after loud noise."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","210"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","International Journal of Audiology"],["dc.bibliographiccitation.lastpage","215"],["dc.bibliographiccitation.volume","51"],["dc.contributor.author","Ihler, Fritz"],["dc.contributor.author","Strieth, Sebastian"],["dc.contributor.author","Pieri, Nicos"],["dc.contributor.author","Goehring, Peter"],["dc.contributor.author","Canis, Martin"],["dc.date.accessioned","2018-11-07T09:13:08Z"],["dc.date.available","2018-11-07T09:13:08Z"],["dc.date.issued","2012"],["dc.description.abstract","Objective: Impairment of microcirculation is a possible cause of sudden sensorineural hearing loss (SSNHL). Fibrinogen is known as a risk factor for both microvascular dysfunction and SSNHL. Therefore, the aim of this study was to investigate the effect of elevated serum levels of fibrinogen on cochlear blood flow and hearing function in vivo. Design: One group of guinea pigs received two consecutive injections of 100 mg fibrinogen while a control group received equimolar doses of albumin. Measurements of cochlear microcirculation by intravital microscopy and of hearing thresholds by auditory brainstem response (ABR) recordings were carried out before, after first and after second injection. Study sample: Ten healthy guinea pigs were randomly assigned to a treatment group or a control group of five animals each. Results: Serum fibrinogen levels were elevated after the first and second injections of fibrinogen compared to basal values and control group respectively. Increasing levels of fibrinogen were paralleled by decreasing cochlear blood flow as well as increasing hearing thresholds. Hearing threshold correlated negatively with cochlear blood flow. Conclusions: The effect of microcirculatory impairment on hearing function could be explained by a malfunction of the cochlear amplifier. Further investigation is needed to quantify cochlear potentials under elevated serum fibrinogen levels."],["dc.identifier.doi","10.3109/14992027.2011.622302"],["dc.identifier.isi","000300491400009"],["dc.identifier.pmid","22332958"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27103"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Informa Healthcare"],["dc.relation.issn","1499-2027"],["dc.title","Acute hyperfibrinogenemia impairs cochlear blood flow and hearing function in guinea pigs in vivo"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e39086"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PloS one"],["dc.bibliographiccitation.lastpage","e39086"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Ihler, Fritz"],["dc.contributor.author","Bertlich, Mattis"],["dc.contributor.author","Sharaf, Kariem"],["dc.contributor.author","Strieth, Sebastian"],["dc.contributor.author","Strupp, Michael"],["dc.contributor.author","Canis, Martin"],["dc.date.accessioned","2019-07-09T11:53:37Z"],["dc.date.available","2019-07-09T11:53:37Z"],["dc.date.issued","2012-06-20"],["dc.description.abstract","OBJECTIVE: Betahistine is a histamine H(1)-receptor agonist and H(3)-receptor antagonist that is administered to treat Menière's disease. Despite widespread use, its pharmacological mode of action has not been entirely elucidated. This study investigated the effect of betahistine on guinea pigs at dosages corresponding to clinically used doses for cochlear microcirculation. METHODS: Thirty healthy Dunkin-Hartley guinea pigs were randomly assigned to five groups to receive betahistine dihydrochloride in a dose of 1,000 mg/kg b. w. (milligram per kilogram body weight), 0.100 mg/kg b. w., 0.010 mg/kg b. w., 0.001 mg/kg b. w. in NaCl 0.9% or NaCl 0.9% alone as placebo. Cochlear blood flow and mean arterial pressure were continuously monitored by intravital fluorescence microscopy and invasive blood pressure measurements 3 minutes before and 15 minutes after administration of betahistine. RESULTS: When betahistine was administered in a dose of 1.000 mg/kg b. w. cochlear blood flow was increased to a peak value of 1.340 arbitrary units (SD: 0.246; range: 0.933-1.546 arb. units) compared to baseline (p<0.05; Two Way Repeated Measures ANOVA/Bonferroni t-test). The lowest dosage of 0.001 mg/kg b. w. betahistine or NaCl 0.9% had the same effect as placebo. Nonlinear regression revealed that there was a sigmoid correlation between increase in blood flow and dosages. CONCLUSIONS: Betahistine has a dose-dependent effect on the increase of blood flow in cochlear capillaries. The effects of the dosage range of betahistine on cochlear microcirculation corresponded well to clinically used single dosages to treat Menière's disease. Our data suggest that the improved effects of higher doses of betahistine in the treatment of Menière's disease might be due to a corresponding increase of cochlear blood flow."],["dc.format.extent","6"],["dc.identifier.doi","10.1371/journal.pone.0039086"],["dc.identifier.fs","593711"],["dc.identifier.pmid","22745706"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7743"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60463"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Betahistine exerts a dose-dependent effect on cochlear stria vascularis blood flow in Guinea pigs in vivo."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]