Thiele, Jan Christoph

[["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","Science Advances"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Jungblut, Marvin"],["dc.contributor.author","Helmerich, Dominic A."],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Chizhik, Anna"],["dc.contributor.author","Chizhik, Alexey I."],["dc.contributor.author","Schnermann, Martin J."],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Enderlein, Jörg"],["dc.creator.author","Jan Christoph Thiele"],["dc.creator.author","Marvin Jungblut"],["dc.creator.author","Dominic A. Helmerich"],["dc.creator.author","Roman Tsukanov"],["dc.creator.author","Anna Chizhik"],["dc.creator.author","Alexey I. Chizhik"],["dc.creator.author","Martin J. Schnermann"],["dc.creator.author","Markus Sauer"],["dc.creator.author","Oleksii Nevskyi"],["dc.creator.author","Jörg Enderlein"],["dc.date.accessioned","2022-06-09T11:30:24Z"],["dc.date.available","2022-06-09T11:30:24Z"],["dc.date.issued","2022"],["dc.description.abstract","Over the past two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution, which is by\r\na factor of 2 to 3 worse. One recently developed method to close this gap is metal-induced energy transfer (MIET)\r\nimaging, which achieves an axial resolution down to nanometers. It exploits the distance-dependent quenching\r\nof fluorescence when a fluorescent molecule is brought close to a metal surface. In the present manuscript, we\r\ncombine the extreme axial resolution of MIET imaging with the extraordinary lateral resolution of single-molecule\r\nlocalization microscopy, in particular with direct stochastic optical reconstruction microscopy (dSTORM). This\r\ncombination allows us to achieve isotropic three-dimensional super-resolution imaging of subcellular structures.\r\nMoreover, we used spectral demixing for implementing dual-color MIET-dSTORM that allows us to image and\r\ncolocalize, in three dimensions, two different cellular structures simultaneously."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.1126/sciadv.abo2506"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/111143"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/504"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY-NC 4.0"],["dc.title","Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]