Behr, Rüdiger

[["dc.bibliographiccitation.firstpage","2498"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Stöckl, Jan B."],["dc.contributor.author","Schmid, Nina"],["dc.contributor.author","Flenkenthaler, Florian"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Mayerhofer, Artur"],["dc.contributor.author","Arnold, Georg J."],["dc.contributor.author","Fröhlich, Thomas"],["dc.date.accessioned","2022-10-06T13:26:49Z"],["dc.date.available","2022-10-06T13:26:49Z"],["dc.date.issued","2020"],["dc.description.abstract","Age-related changes in the human testis may include morphological alterations, disturbed steroidogenesis, and impaired spermatogenesis. However, the specific impact of cell age remains poorly understood and difficult to assess. Testicular peritubular cells fulfill essential functions, including sperm transport, contributions to the spermatogonial stem cell niche, and paracrine interactions within the testis. To study their role in age-associated decline of testicular functions, we performed comprehensive proteome and secretome analyses of repeatedly passaged peritubular cells from Callithrix jacchus. This nonhuman primate model better reflects the human testicular biology than rodents and further gives access to young donors unavailable from humans. Among 5095 identified proteins, 583 were differentially abundant between samples with low and high passage numbers. The alterations indicate a reduced ability of senescent peritubular cells to contract and secrete proteins, as well as disturbances in nuclear factor (NF)-κB signaling and a reduced capacity to handle reactive oxygen species. Since this in vitro model may not exactly mirror all molecular aspects of in vivo aging, we investigated the proteomes and secretomes of testicular peritubular cells from young and old donors. Even though the age-related alterations at the protein level were less pronounced, we found evidence for impaired protein secretion, altered NF-κB signaling, and reduced contractility of these in vivo aged peritubular cells."],["dc.identifier.doi","10.3390/cells9112498"],["dc.identifier.pii","cells9112498"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115175"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","2073-4409"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Proteomic Insights into Senescence of Testicular Peritubular Cells from a Nonhuman Primate Model"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","231"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Reproduction"],["dc.bibliographiccitation.lastpage","238"],["dc.bibliographiccitation.volume","156"],["dc.contributor.author","Walenta, Lena"],["dc.contributor.author","Schmid, Nina"],["dc.contributor.author","Schwarzer, J Ullrich"],["dc.contributor.author","Köhn, Frank-Michael"],["dc.contributor.author","Urbanski, Henryk F"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Strauss, Leena"],["dc.contributor.author","Poutanen, Matti"],["dc.contributor.author","Mayerhofer, Artur"],["dc.date.accessioned","2022-10-06T13:26:27Z"],["dc.date.available","2022-10-06T13:26:27Z"],["dc.date.issued","2018"],["dc.description.abstract","NLRP3 is part of the NLRP3 inflammasome and a global sensor of cellular damage. It was recently discovered in rodent Sertoli cells. We investigated NLRP3 in mouse, human and non-human primate (marmoset and rhesus macaque) testes, employing immunohistochemistry. Sertoli cells of all species expressed NLRP3, and the expression preceded puberty. In addition, peritubular cells of the adult human testes expressed NLRP3.\n NLRP3\n and associated genes (\n PYCARD\n ,\n CASP1\n ,\n IL1B\n ) were also found in isolated human testicular peritubular cells and the mouse Sertoli cell line TM4. Male infertility due to impairments of spermatogenesis may be related to sterile inflammatory events. We observed that the expression of NLRP3 was altered in the testes of patients suffering from mixed atrophy syndrome, in which tubules with impairments of spermatogenesis showed prominent NLRP3 staining. In order to explore a possible role of NLRP3 in male infertility, associated with sterile testicular inflammation, we studied a mouse model of male infertility. These human aromatase-expressing transgenic mice (\n AROM+\n ) develop testicular inflammation and impaired spermatogenesis during aging, and the present data show that this is associated with strikingly elevated\n Nlrp3\n expression in the testes compared to WT controls. Interference by aromatase inhibitor treatment significantly reduced increased\n Nlrp3\n levels. Thus, throughout species NLRP3 is expressed by somatic cells of the testis, which are involved in testicular immune surveillance. We conclude that NLRP3 may be a novel player in testicular immune regulation."],["dc.identifier.doi","10.1530/REP-18-0111"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115091"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1741-7899"],["dc.relation.issn","1470-1626"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","NLRP3 in somatic non-immune cells of rodent and primate testes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cell Death Discovery"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Bagnjuk, Konstantin"],["dc.contributor.author","Stöckl, Jan Bernd"],["dc.contributor.author","Fröhlich, Thomas"],["dc.contributor.author","Arnold, Georg Josef"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Berg, Ulrike"],["dc.contributor.author","Berg, Dieter"],["dc.contributor.author","Kunz, Lars"],["dc.contributor.author","Bishop, Cecily"],["dc.contributor.author","Xu, Jing"],["dc.contributor.author","Mayerhofer, Artur"],["dc.date.accessioned","2022-10-06T13:34:13Z"],["dc.date.available","2022-10-06T13:34:13Z"],["dc.date.issued","2019"],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft https://doi.org/10.13039/501100001659"],["dc.description.sponsorship"," U.S. Department of Health & Human Services | NIH | NIH Office of the Director https://doi.org/10.13039/100000052"],["dc.identifier.doi","10.1038/s41420-019-0149-7"],["dc.identifier.pii","149"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115859"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","2058-7716"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Necroptosis in primate luteolysis: a role for ceramide"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1306"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Stöckl, Jan B."],["dc.contributor.author","Schmid, Nina"],["dc.contributor.author","Flenkenthaler, Florian"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Mayerhofer, Artur"],["dc.contributor.author","Arnold, Georg J."],["dc.contributor.author","Fröhlich, Thomas"],["dc.date.accessioned","2022-10-06T13:26:48Z"],["dc.date.available","2022-10-06T13:26:48Z"],["dc.date.issued","2021"],["dc.description.abstract","Aging of human testis and associated cellular changes is difficult to assess. Therefore, we used a translational, non-human primate model to get insights into underlying cellular and biochemical processes. Using proteomics and immunohistochemistry, we analyzed testicular tissue of young (age 2 to 3) and old (age 10 to 12) common marmosets (Callithrix jacchus). Using a mass spectrometry-based proteomics approach, we identified 63,124 peptides, which could be assigned to 5924 proteins. Among them, we found proteins specific for germ cells and somatic cells, such as Leydig and Sertoli cells. Quantitative analysis showed 31 differentially abundant proteins, of which 29 proteins were more abundant in older animals. An increased abundance of anti-proliferative proteins, among them CDKN2A, indicate reduced cell proliferation in old testes. Additionally, an increased abundance of several small leucine rich repeat proteoglycans and other extracellular matrix proteins was observed, which may be related to impaired cell migration and fibrotic events. Furthermore, an increased abundance of proteins with inhibitory roles in smooth muscle cell contraction like CNN1 indicates functional alterations in testicular peritubular cells and may mirror a reduced capacity of these cells to contract in old testes."],["dc.identifier.doi","10.3390/cells10061306"],["dc.identifier.pii","cells10061306"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115172"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","2073-4409"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Age-Related Alterations in the Testicular Proteome of a Non-Human Primate"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3229"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","The FASEB Journal"],["dc.bibliographiccitation.lastpage","3241"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Hakkarainen, Janne"],["dc.contributor.author","Zhang, Fu-Ping"],["dc.contributor.author","Jokela, Heli"],["dc.contributor.author","Mayerhofer, Artur"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Cisneros-Montalvo, Sheyla"],["dc.contributor.author","Nurmio, Mirja"],["dc.contributor.author","Toppari, Jorma"],["dc.contributor.author","Ohlsson, Claes"],["dc.contributor.author","Kotaja, Noora"],["dc.contributor.author","Poutanen, Matti"],["dc.date.accessioned","2022-10-06T13:35:24Z"],["dc.date.available","2022-10-06T13:35:24Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1096/fj.201700921R"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116090"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1530-6860"],["dc.relation.issn","0892-6638"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Hydroxysteroid (17β) dehydrogenase 1 expressed by Sertoli cells contributes to steroid synthesis and is required for male fertility"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","259"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Reproduction"],["dc.bibliographiccitation.lastpage","268"],["dc.bibliographiccitation.volume","160"],["dc.contributor.author","Schmid, Nina"],["dc.contributor.author","Missel, Annika"],["dc.contributor.author","Petkov, Stoyan"],["dc.contributor.author","Stöckl, Jan B"],["dc.contributor.author","Flenkenthaler, Florian"],["dc.contributor.author","Arnold, Georg J"],["dc.contributor.author","Fröhlich, Thomas"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Mayerhofer, Artur"],["dc.date.accessioned","2022-10-06T13:26:28Z"],["dc.date.available","2022-10-06T13:26:28Z"],["dc.date.issued","2020"],["dc.description.abstract","Testicular peritubular cells (TPCs) are smooth muscle-like cells, which form a compartment surrounding the seminiferous tubules. Previous studies employing isolated human testicular peritubular cells (HTPCs) indicated that their roles in the testis go beyond sperm transport and include paracrine and immunological contributions. Peritubular cells from a non-human primate (MKTPCs), the common marmoset monkey,\n Callithrix jacchus\n , share a high degree of homology with HTPCs. However, like their human counterparts these cells age in vitro and replicative senescence limits in-depth functional or mechanistic studies. Therefore, a stable cellular model was established. MKTPCs of a young adult animal were immortalized by\n piggyBac\n transposition of human telomerase (\n hTERT\n ), that is, without the expression of viral oncogenes. Immortalized MKTPCs (iMKTPCs) grew without discernable changes for more than 50 passages. An initial characterization revealed typical genes expressed by peritubular cells (androgen receptor (\n AR\n ), smooth-muscle actin (\n ACTA2\n ), calponin (\n CNN1\n )). A proteome analysis of the primary MKTPCs and the derived immortalized cell line confirmed that the cells almost completely retained their phenotype. To test whether they respond in a similar way as HTPCs, iMKTPCs were challenged with forskolin (FSK) and ATP. As HTPCs, they showed increased expression level of the StAR protein (\n StAR\n ) after FSK stimulation, indicating steroidogenic capacity. ATP increased the expression of pro-inflammatory factors (e.g.\n IL1B\n ;\n CCL7\n ), as it is the case in HTPCs. Finally, we confirmed that iMKTPCs can efficiently be transfected. Therefore, they represent a highly relevant translational model, which allows mechanistic studies for further exploration of the roles of testicular peritubular cells."],["dc.identifier.doi","10.1530/REP-20-0100"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115093"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1741-7899"],["dc.relation.issn","1470-1626"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","A translational cellular model for the study of peritubular cells of the testis"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]