Behr, Rüdiger

[["dc.bibliographiccitation.artnumber","29122"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.lastpage","13"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Debowski, Katharina"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Lentes, Jana"],["dc.contributor.author","Cors, Maren"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Lingner, Thomas"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Fuchs, Sigrid"],["dc.contributor.author","Sasaki, Erika"],["dc.contributor.author","Behr, Rüdiger"],["dc.date.accessioned","2019-02-20T15:35:19Z"],["dc.date.available","2019-02-20T15:35:19Z"],["dc.date.issued","2016"],["dc.description.abstract","Embryonic stem cells (ESCs) are useful for the study of embryonic development. However, since research on naturally conceived human embryos is limited, non-human primate (NHP) embryos and NHP ESCs represent an excellent alternative to the corresponding human entities. Though, ESC lines derived from naturally conceived NHP embryos are still very rare. Here, we report the generation and characterization of four novel ESC lines derived from natural preimplantation embryos of the common marmoset monkey (Callithrix jacchus). For the first time we document derivation of NHP ESCs derived from morula stages. We show that quantitative chromosome-wise transcriptome analyses precisely reflect trisomies present in both morula-derived ESC lines. We also demonstrate that the female ESC lines exhibit different states of X-inactivation which is impressively reflected by the abundance of the lncRNA X inactive-specific transcript (XIST). The novel marmoset ESC lines will promote basic primate embryo and ESC studies as well as preclinical testing of ESC-based regenerative approaches in NHP."],["dc.identifier.doi","10.1038/srep29122"],["dc.identifier.pmid","27385131"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13499"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/57609"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/149"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C05: Bedeutung von zellulären Immunreaktionen für das kardiale Remodeling und die Therapie der Herzinsuffizienz durch Stammzelltransplantation"],["dc.relation.issn","2045-2322"],["dc.relation.workinggroup","RG Dressel"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","UNSP e0118424"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Debowski, Katharina"],["dc.contributor.author","Warthemann, Rita"],["dc.contributor.author","Lentes, Jana"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Langenstroth, Daniel"],["dc.contributor.author","Gromoll, Joerg"],["dc.contributor.author","Sasaki, Erika"],["dc.contributor.author","Behr, Ruediger"],["dc.date.accessioned","2018-11-07T09:59:32Z"],["dc.date.available","2018-11-07T09:59:32Z"],["dc.date.issued","2015"],["dc.description.abstract","Groundbreaking studies showed that differentiated somatic cells of mouse and human origin could be reverted to a stable pluripotent state by the ectopic expression of only four proteins. The resulting pluripotent cells, called induced pluripotent stem(iPS) cells, could be an alternative to embryonic stem cells, which are under continuous ethical debate. Hence, iPS cell-derived functional cells such as neurons may become the key for an effective treatment of currently incurable degenerative diseases. However, besides the requirement of efficacy testing of the therapy also its long-term safety needs to be carefully evaluated in settings mirroring the clinical situation in an optimal way. In this context, we chose the long-lived common marmoset monkey (Callithrix jacchus) as a non-human primate species to generate iPS cells. The marmoset monkey is frequently used in biomedical research and is gaining more and more preclinical relevance due to the increasing number of disease models. Here, we describe, to our knowledge, the first-time generation of marmoset monkey iPS cells from postnatal skin fibroblasts by non-viral means. We used the transposon-based, fully reversible piggyback system. We cloned the marmoset monkey reprogramming factors and established robust and reproducible reprogramming protocols with a six-factor-in-one-construct approach. We generated six individual iPS cell lines and characterized them in comparison with marmoset monkey embryonic stem cells. The generated iPS cells are morphologically indistinguishable from marmoset ES cells. The iPS cells are fully reprogrammed as demonstrated by differentiation assays, pluripotency marker expression and transcriptome analysis. They are stable for numerous passages (more than 80) and exhibit euploidy. In summary, we have established efficient non-viral reprogramming protocols for the derivation of stable marmoset monkey iPS cells, which can be used to develop and test cell replacement therapies in preclinical settings."],["dc.identifier.doi","10.1371/journal.pone.0118424"],["dc.identifier.isi","000352138500033"],["dc.identifier.pmid","25785453"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11755"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37609"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/125"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C05: Bedeutung von zellulären Immunreaktionen für das kardiale Remodeling und die Therapie der Herzinsuffizienz durch Stammzelltransplantation"],["dc.relation.issn","1932-6203"],["dc.relation.workinggroup","RG Dressel"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Non-Viral Generation of Marmoset Monkey iPS Cells by a Six-Factor-in-One-Vector Approach"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","366"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Developmental cell"],["dc.bibliographiccitation.lastpage","382"],["dc.bibliographiccitation.volume","35"],["dc.contributor.author","Boroviak, Thorsten"],["dc.contributor.author","Loos, Remco"],["dc.contributor.author","Lombard, Patrick"],["dc.contributor.author","Okahara, Junko"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Sasaki, Erika"],["dc.contributor.author","Nichols, Jennifer"],["dc.contributor.author","Smith, Austin"],["dc.contributor.author","Bertone, Paul"],["dc.date.accessioned","2019-07-09T11:41:57Z"],["dc.date.available","2019-07-09T11:41:57Z"],["dc.date.issued","2015-11-09"],["dc.description.abstract","Naive pluripotency is manifest in the preimplantation mammalian embryo. Here we determine transcriptome dynamics of mouse development from the eight-cell stage to postimplantation using lineage-specific RNA sequencing. This method combines high sensitivity and reporter-based fate assignment to acquire the full spectrum of gene expression from discrete embryonic cell types. We define expression modules indicative of developmental state and temporal regulatory patterns marking the establishment and dissolution of naive pluripotency in vivo. Analysis of embryonic stem cells and diapaused embryos reveals near-complete conservation of the core transcriptional circuitry operative in the preimplantation epiblast. Comparison to inner cell masses of marmoset primate blastocysts identifies a similar complement of pluripotency factors but use of alternative signaling pathways. Embryo culture experiments further indicate that marmoset embryos utilize WNT signaling during early lineage segregation, unlike rodents. These findings support a conserved transcription factor foundation for naive pluripotency while revealing species-specific regulatory features of lineage segregation."],["dc.identifier.doi","10.1016/j.devcel.2015.10.011"],["dc.identifier.pmid","26555056"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12621"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58557"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1878-1551"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Lineage-Specific Profiling Delineates the Emergence and Progression of Naive Pluripotency in Mammalian Embryogenesis."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]