Behr, Rüdiger

[["dc.bibliographiccitation.firstpage","2498"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Stöckl, Jan B."],["dc.contributor.author","Schmid, Nina"],["dc.contributor.author","Flenkenthaler, Florian"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Mayerhofer, Artur"],["dc.contributor.author","Arnold, Georg J."],["dc.contributor.author","Fröhlich, Thomas"],["dc.date.accessioned","2022-10-06T13:26:49Z"],["dc.date.available","2022-10-06T13:26:49Z"],["dc.date.issued","2020"],["dc.description.abstract","Age-related changes in the human testis may include morphological alterations, disturbed steroidogenesis, and impaired spermatogenesis. However, the specific impact of cell age remains poorly understood and difficult to assess. Testicular peritubular cells fulfill essential functions, including sperm transport, contributions to the spermatogonial stem cell niche, and paracrine interactions within the testis. To study their role in age-associated decline of testicular functions, we performed comprehensive proteome and secretome analyses of repeatedly passaged peritubular cells from Callithrix jacchus. This nonhuman primate model better reflects the human testicular biology than rodents and further gives access to young donors unavailable from humans. Among 5095 identified proteins, 583 were differentially abundant between samples with low and high passage numbers. The alterations indicate a reduced ability of senescent peritubular cells to contract and secrete proteins, as well as disturbances in nuclear factor (NF)-κB signaling and a reduced capacity to handle reactive oxygen species. Since this in vitro model may not exactly mirror all molecular aspects of in vivo aging, we investigated the proteomes and secretomes of testicular peritubular cells from young and old donors. Even though the age-related alterations at the protein level were less pronounced, we found evidence for impaired protein secretion, altered NF-κB signaling, and reduced contractility of these in vivo aged peritubular cells."],["dc.identifier.doi","10.3390/cells9112498"],["dc.identifier.pii","cells9112498"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115175"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","2073-4409"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Proteomic Insights into Senescence of Testicular Peritubular Cells from a Nonhuman Primate Model"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","175"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of Endocrinology"],["dc.bibliographiccitation.lastpage","187"],["dc.bibliographiccitation.volume","241"],["dc.contributor.author","Wahab, Fazal"],["dc.contributor.author","Khan, Ikram Ullah"],["dc.contributor.author","Polo, Ignacio Rodriguez"],["dc.contributor.author","Zubair, Hira"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Shahab, Muhammad"],["dc.contributor.author","Behr, Rüdiger"],["dc.date.accessioned","2022-10-06T13:26:25Z"],["dc.date.available","2022-10-06T13:26:25Z"],["dc.date.issued","2019"],["dc.description.abstract","Irisin, encoded by the\n FNDC5\n gene, is a recently discovered endocrine factor mainly secreted as a myokine and adipokine. However, irisin/\n FNDC5\n expression has also been reported in different other organs including components of the reproductive axis. Yet, there is the scarcity of data on\n FNDC5\n /irisin expression, regulation and its reproductive effects, particularly in primates. Here, we report the expression of\n FNDC5\n /irisin, along with\n PGC1A\n (peroxisome proliferator-activated receptor gamma coactivator 1-alpha) and\n ERRA\n (estrogen-related receptor alpha), in components of the reproductive axis of marmoset monkeys. Hypothalamic\n FNDC5\n and\n ERRA\n transcript levels are developmentally regulated in both male and female. We further uncovered sex-specific differences in\n FNDC5\n ,\n ERRA\n and\n PGC1A\n expression in muscle and the reproductive axis. Moreover, irisin and ERRα co-localize in the marmoset hypothalamus. Additionally, in the arcuate nucleus of rhesus monkeys, the number of irisin+ cells was significantly increased in short-term fasted monkeys as compared to\n ad libitum\n -fed monkeys. More importantly, we observed putative interaction of irisin-immunoreactive fibers and few GnRH\n -\n immunoreactive cell bodies in the mediobasal hypothalamus of the rhesus monkeys. Functionally, we noted a stimulatory effect of irisin on GnRH synthesis and release in mouse hypothalamic neuronal GT1-7 cells. In summary, our findings show that\n FNDC5\n and irisin are developmentally, metabolic-status dependently and sex-specifically expressed in the primate hypothalamic–pituitary–gonadal axis and exert a stimulatory effect on GnRH expression and release in mouse hypothalamic cells. Further studies are required to confirm the reproductive effects of irisin\n in vivo\n and to illuminate the mechanisms of its regulation."],["dc.identifier.doi","10.1530/JOE-18-0574"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115084"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1479-6805"],["dc.relation.issn","0022-0795"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Irisin in the primate hypothalamus and its effect on GnRH in vitro"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Moussavi, Amir"],["dc.contributor.author","Mietsch, Matthias"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Mylius, Judith"],["dc.contributor.author","Boretius, Susann"],["dc.date.accessioned","2022-10-06T13:34:17Z"],["dc.date.available","2022-10-06T13:34:17Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract\n The aim of this study was to establish a feasible and robust magnetic resonance imaging protocol for the quantitative assessment of cardiac function in marmosets and to present normal values of cardiac function across different ages from young adult, middle-aged, to very old clinically healthy animals. Cardiac MRI of 33 anesthetized marmosets at the age of 2–15 years was performed at 9.4 T using IntraGate-FLASH that operates without any ECG-triggering and breath holding. Normalized to post-mortem heart weight, the left ventricular end-diastolic volume (LV-EDV) was significantly reduced in older marmosets. The LV end-systolic volume (LV-ESV) and the LV stroke volume (LV-SV) showed a similar trend while the LV ejection fraction (LV-EF) and wall thickening remained unchanged. Similar observations were made for the right ventricle. Moreover, the total ventricular myocardial volume was lower in older monkeys while no significant difference in heart weight was found. In conclusion, IntraGate-FLASH allowed for quantification of left ventricular cardiac function but seems to underestimate the volumes of the right ventricle. Although less strong and without significant sex differences, the observed age related changes were similar to previously reported findings in humans supporting marmosets as a model system for age related cardiovascular human diseases."],["dc.identifier.doi","10.1038/s41598-020-67157-5"],["dc.identifier.pii","67157"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115872"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","2045-2322"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Cardiac MRI in common marmosets revealing age-dependency of cardiac function"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","29122"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.lastpage","13"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Debowski, Katharina"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Lentes, Jana"],["dc.contributor.author","Cors, Maren"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Lingner, Thomas"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Fuchs, Sigrid"],["dc.contributor.author","Sasaki, Erika"],["dc.contributor.author","Behr, Rüdiger"],["dc.date.accessioned","2019-02-20T15:35:19Z"],["dc.date.available","2019-02-20T15:35:19Z"],["dc.date.issued","2016"],["dc.description.abstract","Embryonic stem cells (ESCs) are useful for the study of embryonic development. However, since research on naturally conceived human embryos is limited, non-human primate (NHP) embryos and NHP ESCs represent an excellent alternative to the corresponding human entities. Though, ESC lines derived from naturally conceived NHP embryos are still very rare. Here, we report the generation and characterization of four novel ESC lines derived from natural preimplantation embryos of the common marmoset monkey (Callithrix jacchus). For the first time we document derivation of NHP ESCs derived from morula stages. We show that quantitative chromosome-wise transcriptome analyses precisely reflect trisomies present in both morula-derived ESC lines. We also demonstrate that the female ESC lines exhibit different states of X-inactivation which is impressively reflected by the abundance of the lncRNA X inactive-specific transcript (XIST). The novel marmoset ESC lines will promote basic primate embryo and ESC studies as well as preclinical testing of ESC-based regenerative approaches in NHP."],["dc.identifier.doi","10.1038/srep29122"],["dc.identifier.pmid","27385131"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13499"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/57609"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/149"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C05: Bedeutung von zellulären Immunreaktionen für das kardiale Remodeling und die Therapie der Herzinsuffizienz durch Stammzelltransplantation"],["dc.relation.issn","2045-2322"],["dc.relation.workinggroup","RG Dressel"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","505"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Vogt, Edgar-John"],["dc.contributor.author","Heistermann, Michael"],["dc.contributor.author","Roshani, Berit"],["dc.contributor.author","Becker, Tamara"],["dc.contributor.author","Mätz-Rensing, Kerstin"],["dc.contributor.author","Kues, Wilfried A."],["dc.contributor.author","Kügler, Sebastian"],["dc.contributor.author","Behr, Rüdiger"],["dc.date.accessioned","2021-06-01T09:42:32Z"],["dc.date.available","2021-06-01T09:42:32Z"],["dc.date.issued","2021"],["dc.description.abstract","Genetic modification of non-human primates (NHP) paves the way for realistic disease models. The common marmoset is a NHP species increasingly used in biomedical research. Despite the invention of RNA-guided nucleases, one strategy for protein overexpression in NHP is still lentiviral transduction. We generated three male and one female enhanced green fluorescent protein (EGFP)-transgenic founder marmosets via lentiviral transduction of natural preimplantation embryos. All founders accomplished germline transmission of the transgene by natural mating, yielding 20 transgenic offspring together (in total, 45 pups; 44% transgenic). This demonstrates that the transgenic gametes are capable of natural fertilization even when in competition with wildtype gametes. Importantly, 90% of the transgenic offspring showed transgene silencing, which is in sharp contrast to rodents, where the identical transgene facilitated robust EGFP expression. Furthermore, we consistently discovered somatic, but so far, no germ cell chimerism in mixed wildtype/transgenic litters. Somatic cell chimerism resulted in false-positive genotyping of the respective wildtype littermates. For the discrimination of transgenic from transgene-chimeric animals by polymerase chain reaction on skin samples, a chimeric cell depletion protocol was established. In summary, it is possible to establish a cohort of genetically modified marmosets by natural mating, but specific requirements including careful promoter selection are essential."],["dc.identifier.doi","10.3390/cells10030505"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85277"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","2073-4409"],["dc.title","Generation and Breeding of EGFP-Transgenic Marmoset Monkeys: Cell Chimerism and Implications for Disease Modeling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","110670"],["dc.bibliographiccitation.journal","Molecular and Cellular Endocrinology"],["dc.bibliographiccitation.volume","504"],["dc.contributor.author","Wahab, Fazal"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Mätz-Rensing, Kerstin"],["dc.contributor.author","Fuchs, Eberhard"],["dc.contributor.author","Behr, Rüdiger"],["dc.date.accessioned","2022-10-06T13:33:14Z"],["dc.date.available","2022-10-06T13:33:14Z"],["dc.date.issued","2020"],["dc.description.sponsorship"," http://dx.doi.org/10.13039/100005156 Alexander von Humboldt Foundation"],["dc.description.sponsorship"," http://dx.doi.org/10.13039/501100004938 German Primate Center"],["dc.identifier.doi","10.1016/j.mce.2019.110670"],["dc.identifier.pii","S0303720719303727"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115584"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.issn","0303-7207"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","Irisin is expressed by undifferentiated spermatogonia and modulates gene expression in organotypic primate testis cultures"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2414"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Animals"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Münzker, Julia"],["dc.contributor.author","Heistermann, Michael"],["dc.contributor.author","Becker, Tamara"],["dc.contributor.author","Mißbach, Sophie"],["dc.contributor.author","Behr, Rüdiger"],["dc.date.accessioned","2022-10-04T10:21:49Z"],["dc.date.available","2022-10-04T10:21:49Z"],["dc.date.issued","2022"],["dc.description.abstract","Non-human primates (NHPs) serve as embryo donors for embryo collection in order to mimic genetic diseases in humans by genetic modification. Reproductive health of the embryo donors is crucial, and chronic distress needs to be avoided. Embryo retrieval rates (ERR), anti-Müllerian hormone (AMH) concentrations, cortisol levels, and body weight fluctuations were assessed as markers for fertility and distress. With regard to successful embryo retrievals (total n = 667), the animals were either used for extended periods (long-term group; LTG) or only for short periods (short-term group; STG). Retrospective evaluation expectedly showed that animals in the LTG had a higher ERR than animals in the STG (p < 0.0001). Importantly, ERR in the LTG remained stable throughout the experimental period, and high embryo rates were already encountered during the first year of experimental use (p = 0.0002). High ERR were associated with high AMH and low cortisol levels, and minimal body weight fluctuations following anesthesia, indicating a superior ability of the LTG animals to handle distress. We conclude that the long-term experimental use of marmosets does not impair their fertility or health status per se, supporting the view that animal reuse can be in accordance with the 3R-principle, implying reduction, replacement, and refinement in animal experimentation."],["dc.identifier.doi","10.3390/ani12182414"],["dc.identifier.pii","ani12182414"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114507"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.eissn","2076-2615"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Performance of Marmoset Monkeys as Embryo Donors Is Reflected by Different Stress-Related Parameters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1306"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Stöckl, Jan B."],["dc.contributor.author","Schmid, Nina"],["dc.contributor.author","Flenkenthaler, Florian"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Mayerhofer, Artur"],["dc.contributor.author","Arnold, Georg J."],["dc.contributor.author","Fröhlich, Thomas"],["dc.date.accessioned","2022-10-06T13:26:48Z"],["dc.date.available","2022-10-06T13:26:48Z"],["dc.date.issued","2021"],["dc.description.abstract","Aging of human testis and associated cellular changes is difficult to assess. Therefore, we used a translational, non-human primate model to get insights into underlying cellular and biochemical processes. Using proteomics and immunohistochemistry, we analyzed testicular tissue of young (age 2 to 3) and old (age 10 to 12) common marmosets (Callithrix jacchus). Using a mass spectrometry-based proteomics approach, we identified 63,124 peptides, which could be assigned to 5924 proteins. Among them, we found proteins specific for germ cells and somatic cells, such as Leydig and Sertoli cells. Quantitative analysis showed 31 differentially abundant proteins, of which 29 proteins were more abundant in older animals. An increased abundance of anti-proliferative proteins, among them CDKN2A, indicate reduced cell proliferation in old testes. Additionally, an increased abundance of several small leucine rich repeat proteoglycans and other extracellular matrix proteins was observed, which may be related to impaired cell migration and fibrotic events. Furthermore, an increased abundance of proteins with inhibitory roles in smooth muscle cell contraction like CNN1 indicates functional alterations in testicular peritubular cells and may mirror a reduced capacity of these cells to contract in old testes."],["dc.identifier.doi","10.3390/cells10061306"],["dc.identifier.pii","cells10061306"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115172"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","2073-4409"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Age-Related Alterations in the Testicular Proteome of a Non-Human Primate"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Aging Cell"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Potabattula, Ramya"],["dc.contributor.author","Zacchini, Federica"],["dc.contributor.author","Ptak, Grazyna Ewa"],["dc.contributor.author","Dittrich, Marcus"],["dc.contributor.author","Müller, Tobias"],["dc.contributor.author","El Hajj, Nady"],["dc.contributor.author","Hahn, Thomas"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Lucas‐Hahn, Andrea"],["dc.contributor.author","Haaf, Thomas"],["dc.date.accessioned","2022-10-06T13:24:59Z"],["dc.date.available","2022-10-06T13:24:59Z"],["dc.date.issued","2020"],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.description.sponsorship"," Horizon 2020 Framework Programme http://dx.doi.org/10.13039/100010661"],["dc.identifier.doi","10.1111/acel.13181"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114719"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1474-9726"],["dc.relation.issn","1474-9718"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Increasing methylation of sperm rDNA and other repetitive elements in the aging male mammalian germline"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","256"],["dc.bibliographiccitation.issue","5-6"],["dc.bibliographiccitation.journal","Cells Tissues Organs"],["dc.bibliographiccitation.lastpage","278"],["dc.bibliographiccitation.volume","205"],["dc.contributor.author","Rulle, Alexander"],["dc.contributor.author","Tsikolia, Nikoloz"],["dc.contributor.author","de Bakker, Bernadette"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Behr, Rüdiger"],["dc.contributor.author","Viebahn, Christoph"],["dc.date.accessioned","2019-11-11T12:49:20Z"],["dc.date.available","2019-11-11T12:49:20Z"],["dc.date.issued","2018"],["dc.description.abstract","Existence and biomedical relevance of the neurenteric canal, a transient midline structure during early neurulation in the human embryo, have been controversially discussed for more than a century by embryologists and clinicians alike. In this study, the authors address the long-standing enigma by high-resolution histology and three-dimensional reconstruction using new and historic histological sections of 5 human 17- to 21-day-old embryos and of 2 marmoset monkey embryos of the species Callithrix jacchus at corresponding stages. The neurenteric canal presents itself as the classical vertical connection between the amniotic cavity and the yolk sac cavity and is lined (a) craniolaterally by a horseshoe-shaped \"hinge of involuting notochordal cells\" within Hensen's node and (b) caudally by the receding primitive streak epiblast dorsally and by notochordal plate epithelium ventrally, the latter of which covered the (longitudinal) notochordal canal on its ventral side at the preceding stage. Furthermore, asymmetric parachordal nodal expression in Callithrix and morphological asymmetries within the nodes of the other specimens suggest an early non-cilium-dependent left-right symmetry breaking mode previously postulated for other mammals. We conclude that structure and position of the mammalian neurenteric canal support the notion of its homology with the reptilian blastopore as a whole and with a dorsal segment of the blastopore in amphibia. These new features of the neurenteric canal may further clarify the aetiology of foetal malformations such as junctional neurulation defects, neuroendodermal cysts, and the split notochord syndrome."],["dc.identifier.doi","10.1159/000493276"],["dc.identifier.pmid","30481762"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62595"],["dc.language.iso","en"],["dc.relation.eissn","1422-6421"],["dc.relation.issn","1422-6405"],["dc.relation.issn","1422-6421"],["dc.title","On the Enigma of the Human Neurenteric Canal"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]