Priebe, Marius

[["dc.bibliographiccitation.firstpage","680"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","690"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Bernhardt, Marten"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Wollnik, Bernd"],["dc.contributor.author","Diaz, Ana"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Rehfeldt, Florian"],["dc.date.accessioned","2017-09-07T11:54:39Z"],["dc.date.available","2017-09-07T11:54:39Z"],["dc.date.issued","2016"],["dc.description.abstract","Adult human mesenchymal stem cells show structural rearrangements of their cytoskeletal network during mechanically induced differentiation toward various cell types. In particular, the alignment of acto-myosin fibers is cell fate-dependent and can serve as an early morphological marker of differentiation. Quantification of such nanostructures on a mesoscopic scale requires high-resolution imaging techniques. Here, we use small-angle x-ray scattering with a spot size in the micro- and submicrometer range as a high-resolution and label-free imaging technique to reveal structural details of stem cells and differentiated cell types. We include principal component analysis into an automated empirical analysis scheme that allows the local characterization of oriented structures. Results on freeze-dried samples lead to quantitative structural information for all cell lines tested: differentiated cells reveal pronounced structural orientation and a relatively intense overall diffraction signal, whereas naive human mesenchymal stem cells lack these features. Our data support the hypothesis of stem cells establishing ordered structures along their differentiation process."],["dc.identifier.doi","10.1016/j.bpj.2015.12.017"],["dc.identifier.gro","3141731"],["dc.identifier.isi","000369467800017"],["dc.identifier.pmid","26840732"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14077"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/446"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","X-Ray Micro- and Nanodiffraction Imaging on Human Mesenchymal Stem Cells and Differentiated Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e90884"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Gorniak, Thomas"],["dc.contributor.author","Haraszti, Tamas"],["dc.contributor.author","Garamus, Vasyl M."],["dc.contributor.author","Buck, Andreas R."],["dc.contributor.author","Senkbeil, Tobias"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Hedberg-Buenz, Adam"],["dc.contributor.author","Koehn, Demelza"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Grunze, Michael"],["dc.contributor.author","Anderson, Michael G."],["dc.contributor.author","Rosenhahn, Axel"],["dc.date.accessioned","2017-09-07T11:46:25Z"],["dc.date.available","2017-09-07T11:46:25Z"],["dc.date.issued","2014"],["dc.description.abstract","Melanosomes are highly specialized organelles that produce and store the pigment melanin, thereby fulfilling essential functions within their host organism. Besides having obvious cosmetic consequences-determining the color of skin, hair and the iris-they contribute to photochemical protection from ultraviolet radiation, as well as to vision (by defining how much light enters the eye). Though melanosomes can be beneficial for health, abnormalities in their structure can lead to adverse effects. Knowledge of their ultrastructure will be crucial to gaining insight into the mechanisms that ultimately lead to melanosome-related diseases. However, due to their small size and electron-dense content, physiologically intact melanosomes are recalcitrant to study by common imaging techniques such as light and transmission electron microscopy. In contrast, X-ray-based methodologies offer both high spatial resolution and powerful penetrating capabilities, and thus are well suited to study the ultrastructure of electron-dense organelles in their natural, hydrated form. Here, we report on the application of small-angle X-ray scattering-a method effective in determining the three-dimensional structures of biomolecules - to whole, hydrated murine melanosomes. The use of complementary information from the scattering signal of a large ensemble of suspended organelles and from single, vitrified specimens revealed a melanosomal sub-structure whose surface and bulk properties differ in two commonly used inbred strains of laboratory mice. Whereas melanosomes in C57BL/6J mice have a well-defined surface and are densely packed with 40-nm units, their counterparts in DBA/2J mice feature a rough surface, are more granular and consist of 60-nm building blocks. The fact that these strains have different coat colors and distinct susceptibilities to pigment-related eye disease suggest that these differences in size and packing are of biological significance."],["dc.identifier.doi","10.1371/journal.pone.0090884"],["dc.identifier.fs","606505"],["dc.identifier.gro","3142172"],["dc.identifier.isi","000332845300034"],["dc.identifier.pmid","24621581"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10014"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5332"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray scattering"],["dc.title","Nano-Scale Morphology of Melanosomes Revealed by Small-Angle X-Ray Scattering"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2662"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2673"],["dc.bibliographiccitation.volume","107"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Bernhardt, Marten"],["dc.contributor.author","Blum, Christoph"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:45:22Z"],["dc.date.available","2017-09-07T11:45:22Z"],["dc.date.issued","2014"],["dc.description.abstract","We have performed scanning x-ray nanobeam diffraction experiments on single cells of the amoeba Dictyostelium discoideum. Cells have been investigated in 1), freeze-dried, 2), frozen-hydrated (vitrified), and 3), initially alive states. The spatially resolved small-angle x-ray scattering signal shows characteristic streaklike patterns in reciprocal space, which we attribute to fiber bundles of the actomyosin network. From the intensity distributions, an anisotropy parameter can be derived that indicates pronounced local variations within the cell. In addition to nanobeam small-angle x-ray scattering, we have evaluated the x-ray differential phase contrast in view of the projected electron density. Different experimental aspects of the x-ray experiment, sample preparation, and data analysis are discussed. Finally, the x-ray results are correlated with optical microscopy (differential phase contrast and confocal microscopy of mutant strains with fluorescently labeled actin and myosin II), which have been carried out in live and fixed states, including optical microscopy under cryogenic conditions."],["dc.identifier.doi","10.1016/j.bpj.2014.10.027"],["dc.identifier.fs","606778"],["dc.identifier.gro","3142000"],["dc.identifier.isi","000345859500026"],["dc.identifier.pmid","25468345"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12127"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3434"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft [A11]; Sonderforschungsbereich [937]"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY-NC-ND 3.0"],["dc.rights.uri","http://creativecommons.org/licenses/by-nc-nd/3.0/"],["dc.subject.gro","x-ray scattering"],["dc.subject.mesh","Anisotropy"],["dc.subject.mesh","Cell Survival"],["dc.subject.mesh","Dictyostelium"],["dc.subject.mesh","Nanoparticles"],["dc.subject.mesh","Scattering, Small Angle"],["dc.subject.mesh","Software"],["dc.subject.mesh","X-Ray Diffraction"],["dc.title","Scanning X-Ray Nanodiffraction on Dictyostelium discoideum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","10"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Optical Nanoscopy"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Krüger, Sven P"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Olendrowitz, Christian"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:54:07Z"],["dc.date.available","2017-09-07T11:54:07Z"],["dc.date.issued","2012"],["dc.description.abstract","We have imaged the three-dimensional density distribution of unstained and unsliced, freeze-dried cells of the gram-positive bacterium Deinococcus radiodurans by tomographic x-ray propagation microscopy, i.e. projection tomography with phase contrast formation by free space propagation. The work extends previous x-ray imaging of biological cells in the simple in-line holography geometry to full three-dimensional reconstruction, based on a fast iterative phase reconstruction algorithm which circumvents the usual twin-image problem. The sample is illuminated by the highly curved wave fronts emitted from a virtual quasi-point source with 10 nm cross section, realized by two crossed x-ray waveguides. The experimental scheme allows for a particularly dose efficient determination of the 3D density distribution in the cellular structure."],["dc.identifier.doi","10.1186/2192-2853-1-10"],["dc.identifier.fs","593648"],["dc.identifier.gro","3145116"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9581"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2817"],["dc.language.iso","en"],["dc.notes","Funding by the DFG collaborative research center SFB 755\r\nNanoscale Photonic Imaging and the German Ministry of Education and\r\nResearch (Grant No. 05K10MGA) is gratefully acknowledged."],["dc.notes.intern","Crossref Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.issn","2192-2853"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Low-dose three-dimensional hard x-ray imaging of bacterial cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","043056"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","New Journal of Physics"],["dc.bibliographiccitation.lastpage","13"],["dc.bibliographiccitation.volume","12"],["dc.contributor.affiliation","Priebe, M;"],["dc.contributor.affiliation","Kalbfleisch, S;"],["dc.contributor.affiliation","Tolkiehn, M;"],["dc.contributor.affiliation","Köster, S;"],["dc.contributor.affiliation","Abel, B;"],["dc.contributor.affiliation","Davies, R J;"],["dc.contributor.affiliation","Salditt, T;"],["dc.contributor.author","Kalbfleisch, S"],["dc.contributor.author","Tolkiehn, M"],["dc.contributor.author","Köster, Sarah"],["dc.contributor.author","Davies, R J"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Abel, Bernd"],["dc.date.accessioned","2022-10-11T05:21:38Z"],["dc.date.available","2022-10-11T05:21:38Z"],["dc.date.issued","2010"],["dc.date.updated","2022-02-09T22:15:33Z"],["dc.description.abstract","We have investigated multilamellar lipid assemblies in a microfluidic jet, operating at high shear rates of the order of 10(7) s(-1). Compared to classical Couette cells or rheometers, the shear rate was increased by at least 2-3 orders of magnitude, and the sample volume was scaled down correspondingly. At the same time, the jet is characterized by high extensional stress due to elongational flow. A focused synchrotron x-ray beam was used to measure the structure and orientation of the lipid assemblies in the jet. The diffraction patterns indicate conventional multilamellar phases, aligned with the membrane normals oriented along the velocity gradient of the jet. The results indicate that the setup may be well suited for coherent diffractive imaging of oriented biomolecular assemblies and macromolecules at the future x-ray free electron laser (XFEL) sources."],["dc.identifier.doi","10.1088/1367-2630/12/4/043056"],["dc.identifier.eissn","1367-2630"],["dc.identifier.fs","569048"],["dc.identifier.gro","3142934"],["dc.identifier.isi","000277355200005"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6683"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116225"],["dc.identifier.url","https://publications.goettingen-research-online.de/handle/2/393"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1367-2630"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://publishingsupport.iopscience.iop.org/open_access/"],["dc.subject.gro","molecular biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Orientation of biomolecular assemblies in a microfluidic jet"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","19232"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","19254"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Diaz, Ana"],["dc.contributor.author","Karvinen, P."],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:48:27Z"],["dc.date.available","2017-09-07T11:48:27Z"],["dc.date.issued","2012"],["dc.description.abstract","Ptychographic coherent X-ray diffractive imaging (PCDI) has been combined with nano-focus X-ray diffraction to study the structure and density distribution of unstained and unsliced bacterial cells, using a hard X-ray beam of 6.2keV photon energy, focused to about 90nm by a Fresnel zone plate lens. While PCDI provides images of the bacteria with quantitative contrast in real space with a resolution well below the beam size at the sample, spatially resolved small angle X-ray scattering using the same Fresnel zone plate (cellular nano-diffraction) provides structural information at highest resolution in reciprocal space up to 2nm(-1). We show how the real and reciprocal space approach can be used synergistically on the same sample and with the same setup. In addition, we present 3D hard X-ray imaging of unstained bacterial cells by a combination of ptychography and tomography. (C) 2012 Optical Society of America"],["dc.identifier.doi","10.1364/OE.20.019232"],["dc.identifier.fs","589591"],["dc.identifier.gro","3142482"],["dc.identifier.isi","000307873600075"],["dc.identifier.pmid","23038565"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9566"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8773"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft [SFB 755]"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Hard X-ray imaging of bacterial cells: nano-diffraction and ptychographic reconstruction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]