Now showing 1 - 3 of 3
  • 2021Journal Article
    [["dc.bibliographiccitation.firstpage","6070"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Chemistry – A European Journal"],["dc.bibliographiccitation.lastpage","6076"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Grimm, Florian"],["dc.contributor.author","Rehman, Jasmin"],["dc.contributor.author","Stoldt, Stefan"],["dc.contributor.author","Khan, Taukeer A."],["dc.contributor.author","Schlötel, Jan Gero"],["dc.contributor.author","Nizamov, Shamil"],["dc.contributor.author","John, Michael"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2021-06-01T09:41:16Z"],["dc.date.available","2021-06-01T09:41:16Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1002/chem.202005134"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/84865"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1521-3765"],["dc.relation.issn","0947-6539"],["dc.title","Rhodamines with a Chloronicotinic Acid Fragment for Live Cell Superresolution STED Microscopy "],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]
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  • 2022Journal Article
    [["dc.bibliographiccitation.journal","Nature Biotechnology"],["dc.contributor.author","Weber, Michael"],["dc.contributor.author","von der Emde, Henrik"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Gunkel, Philip"],["dc.contributor.author","Sambandan, Sivakumar"],["dc.contributor.author","Khan, Taukeer A."],["dc.contributor.author","Keller-Findeisen, Jan"],["dc.contributor.author","Cordes, Volker C."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-12-01T08:30:53Z"],["dc.date.available","2022-12-01T08:30:53Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract\n \n Super-resolution techniques have achieved localization precisions in the nanometer regime. Here we report all-optical, room temperature localization of fluorophores with precision in the Ångström range. We built on the concept of MINSTED nanoscopy where precision is increased by encircling the fluorophore with the low-intensity central region of a stimulated emission depletion (STED) donut beam while constantly increasing the absolute donut power. By blue-shifting the STED beam and separating fluorophores by on/off switching, individual fluorophores bound to a DNA strand are localized with\n σ\n  = 4.7 Å, corresponding to a fraction of the fluorophore size, with only 2,000 detected photons. MINSTED fluorescence nanoscopy with single-digit nanometer resolution is exemplified by imaging nuclear pore complexes and the distribution of nuclear lamin in mammalian cells labeled by transient DNA hybridization. Because our experiments yield a localization precision\n σ\n  = 2.3 Å, estimated for 10,000 detected photons, we anticipate that MINSTED will open up new areas of application in the study of macromolecular complexes in cells."],["dc.identifier.doi","10.1038/s41587-022-01519-4"],["dc.identifier.pii","1519"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118008"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","1546-1696"],["dc.relation.issn","1087-0156"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","MINSTED nanoscopy enters the Ångström localization range"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]
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  • 2020Journal Article
    [["dc.bibliographiccitation.firstpage","451"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Chemistry – A European Journal"],["dc.bibliographiccitation.lastpage","458"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Weber, Michael"],["dc.contributor.author","Khan, Taukeer A."],["dc.contributor.author","Patalag, Lukas J."],["dc.contributor.author","Bossi, Mariano"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:45:14Z"],["dc.date.available","2022-03-01T11:45:14Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1002/chem.202004645"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103260"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1521-3765"],["dc.relation.issn","0947-6539"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Photoactivatable Fluorophore for Stimulated Emission Depletion (STED) Microscopy and Bioconjugation Technique for Hydrophobic Labels"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]
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