Stuermer, Ewa Klara

[["dc.bibliographiccitation.firstpage","317"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Cells Tissues Organs"],["dc.bibliographiccitation.lastpage","326"],["dc.bibliographiccitation.volume","189"],["dc.contributor.author","Drengk, Anja"],["dc.contributor.author","Zapf, Antonia"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.contributor.author","Stuermer, Klaus Michael"],["dc.contributor.author","Frosch, Karl-Heinz"],["dc.date.accessioned","2018-11-07T08:34:01Z"],["dc.date.available","2018-11-07T08:34:01Z"],["dc.date.issued","2009"],["dc.description.abstract","Background/Aims: Autologous chondrocyte (CC) transplantation has the disadvantages of requiring two surgical interventions and in vitro expansion of cells, implying the risk of cellular dedifferentiation. Our clinical aim is to develop a one-step procedure for autologous CC transplantation, i.e. harvesting, isolation and reimplantation of CC performed in one single surgical procedure. Platelet-rich plasma (PRP) is a source of autologous growth factors reported to have mitogenic effects. The objective of this study was to test the influence of PRP as an autologous scaffold on freshly isolated CC and mesenchymal stem cells (MSC). Methods: CC and MSC were subjected to two- or three-dimensional (3D) growth systems, either with or without PRP. Chondrogenic differentiation was determined via quantification of collagen type II mRNA and immunohistochemical staining. Results: We observed a proliferative effect for MSCs exposed to PRP in monolayer culture and an increase in the expression of chondrogenic markers when cells are exposed to a 3D environment. CCs exposed to PRP show a decrease in the chondrogenic phenotype with increasing proliferative activity. Conclusion: PRP has a proliferative effect on CCs and MSCs. In a one-step procedure for autologous CC transplantation, this might be an advantage over other scaffold materials, but confirmation in in vivo studies is required. Copyright (C) 2008 S. Karger AG, Basel"],["dc.identifier.doi","10.1159/000151290"],["dc.identifier.isi","000265178600002"],["dc.identifier.pmid","18689989"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9312"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17722"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.relation.issn","1422-6405"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Influence of Platelet-Rich Plasma on Chondrogenic Differentiation and Proliferation of Chondrocytes and Mesenchymal Stem Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Cytotherapy"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Frosch, Karl-Heinz"],["dc.contributor.author","Hingelbaum, S."],["dc.contributor.author","Drengk, Anja"],["dc.contributor.author","Jennissen, H. P."],["dc.contributor.author","Chatzinikolaidou, M."],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.contributor.author","Stuermer, Klaus-Michael"],["dc.date.accessioned","2018-11-07T10:32:54Z"],["dc.date.available","2018-11-07T10:32:54Z"],["dc.date.issued","2006"],["dc.format.extent","8"],["dc.identifier.isi","000239337800021"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/44468"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Taylor & Francis As"],["dc.publisher.place","Oslo"],["dc.relation.issn","1465-3249"],["dc.title","BMP-2 and cell-coated titanium implants accelerate healing of osteochondral defects in a sheep model"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","850"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Planta Medica"],["dc.bibliographiccitation.lastpage","857"],["dc.bibliographiccitation.volume","76"],["dc.contributor.author","Kolios, Leila"],["dc.contributor.author","Schumann, Jacob"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Rack, Thomas"],["dc.contributor.author","Tezval, Mohammed"],["dc.contributor.author","Seidlova-Wuttke, Dana"],["dc.contributor.author","Frosch, Karl-Heinz"],["dc.contributor.author","Stuermer, Klaus Michael"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.date.accessioned","2018-11-07T08:43:01Z"],["dc.date.available","2018-11-07T08:43:01Z"],["dc.date.issued","2010"],["dc.description.abstract","Osteoporosis and its accompanying, predominantly metaphyseal, fractures are a major health problem. Black cohosh (Cimicifuga racemosa) and estrogen positively influence osteoporotic bone. Both substances may improve fracture healing in early osteoporosis as well. In 48 twelve-week-old ovariectomized or, respectively, sham-operated (SHAM) rats, a standardized metaphyseal tibia osteotomy with bridging T-plate fixation was performed. During the healing process of 35 days, rats received soy-free (SHAM, osteopenic C), estrogen-(E) or Cimicifuga racemosa-(CR) supplemented diets. After sacrifice, the callus formation was analyzed with regard to biomechanical quality, morphology, quantity, time course of new bone built and gene expression. CR induced a high rate of metaphyseal callus formation. The biomechanical properties and the amount of new callus formation indicated that fracture healing was still in progress. Therefore, gene expression of osteoblasts was comparatively high. Body weight and the trabecular structure were influenced little by CR. Estrogen improved the biomechanical properties of the callus. Resistance to microfracturing was significantly enhanced in the E group and even superior to SHAM. Remodeling of the callus formation had already begun. The trabecular network and the typical endosteal fracture healing were especially improved. Osteoporotic metaphyseal fracture healing was improved by estrogen more than by Cimicifuga racemosa. The process of fracture healing occurred nearly physiologically. The generation of callus formation was supported by Cimicifuga racemosa as well, but the five-week duration of application was too short for Cimicifuga racemosa to show its complete potential. Already-initiated Cimicifuga racemosa therapy for menopausal symptoms could be continued during fracture healing without hesitation."],["dc.description.sponsorship","DFG [STU 478/2-1]"],["dc.identifier.doi","10.1055/s-0029-1240798"],["dc.identifier.isi","000279668400002"],["dc.identifier.pmid","20104444"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19846"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Georg Thieme Verlag Kg"],["dc.relation.issn","0032-0943"],["dc.title","Effects of Black Cohosh (Cimicifuga racemosa) and Estrogen on Metaphyseal Fracture Healing in the Early Stage of Osteoporosis in Ovariectomized Rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","187"],["dc.bibliographiccitation.journal","Bone"],["dc.bibliographiccitation.lastpage","194"],["dc.bibliographiccitation.volume","64"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.contributor.author","Komrakova, Marina"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Schaefer, Nadine"],["dc.contributor.author","Hallecker, Jan"],["dc.contributor.author","Stuermer, Klaus-Michael"],["dc.date.accessioned","2018-11-07T09:38:39Z"],["dc.date.available","2018-11-07T09:38:39Z"],["dc.date.issued","2014"],["dc.description.abstract","Current osteoporosis therapies aim to delay bone destruction and have additional anabolic effects. While they have demonstrated some positive effects on bone healing, more progress is needed in this area. This study used the well-known osteoporotic agents estrogen (E) and raloxifene (R) in conjunction with biomechanical whole body vibration (WBV) at a frequency of 70 Hz twice daily for six weeks to stimulate bone healing. Eighty-four 3-month old female Sprague-Dawley rats (12 per group) were bilaterally ovariectomized to develop osteopenia within eight weeks. Osteotomy of the metaphyseal tibiae was performed and fracture healing was then studied using mechanical tests, histomorphometry, computed tomography (mu CT), and gene analysis. We found that E and R improved the structure of osteopenic bones as did WBV alone, although significant levels for WBV were seldom reached. Combination treatments significantly enhanced stiffness (R + WBV; p < 0.05), endosteal bone (R + WBV; p < 0.01), and trabecular density (E + WBV; p < 0.05, R + WBV; p < 0.05). In addition, the expression of osteoclast-specific Trap was significantly reduced after treatment with E, R, or their combination with WBV (p < 0.01). The effects were additive and not inhibitory, leading us to conclude that the combined applications of WBV with E or R may improve the healing of osteopenic bones. The therapies studied are all currently approved for human use, suggesting ready applicability to clinical practice. To better understand the effects of WBV on osteopenic bones, the ideal vibration regime will require further study. (C) 2014 Elsevier Inc. All rights reserved."],["dc.description.sponsorship","German Research Foundation (DFG) [STU 478/3-1]"],["dc.identifier.doi","10.1016/j.bone.2014.04.008"],["dc.identifier.isi","337011500026"],["dc.identifier.pmid","24735975"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33112"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","1873-2763"],["dc.relation.issn","8756-3282"],["dc.title","Whole body vibration during fracture healing intensifies the effects of estradiol and raloxifene in estrogen-deficient rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","331"],["dc.bibliographiccitation.issue","5-6"],["dc.bibliographiccitation.journal","Journal of Molecular Histology"],["dc.bibliographiccitation.lastpage","341"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","Tezval, Mohammad"],["dc.contributor.author","Tezval, Hossein"],["dc.contributor.author","Dresing, Klaus"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.contributor.author","Blaschke, Martina"],["dc.contributor.author","Stuermer, Klaus Michael"],["dc.contributor.author","Siggelkow, Heide"],["dc.date.accessioned","2018-11-07T11:23:52Z"],["dc.date.available","2018-11-07T11:23:52Z"],["dc.date.issued","2009"],["dc.description.abstract","Urocortin-1 (UCN) a corticotropin releasing-factor (CRF) related peptide, has been found to be expressed in many different tissues like the central nervous system, the cardiovascular system, adipose tissue, and skeletal muscle. The effects of UCN are mediated via stimulation of CRF-receptors 1 and 2 (CRFR1 and 2, CRFR's) with a high affinity for CRFR2. It has been shown that the CRF-related peptides and CRFR's are involved in the regulation of stress-related endocrine, autonomic and behavioural responses. Using immunocytochemistry, immunohistochemistry and RT-PCR, we now can show the differentiation dependent expression of UCN mRNA and peptide in human mesenchymal progenitor cells (MSCs) directed to the osteoblastic phenotype for the first time. UCN expression was down regulated by TGF-beta and BMP-2 in the early proliferation phase of osteoblast development, whereas dexamethasone (dex) minimally induced UCN gene expression during matrix maturation after 24 h stimulation. Stimulation of MSCs for 28 days with ascorbate/beta-glycerophosphate (asc/bGp) induced UCN gene expression at day 14. This effect was prevented when using 1,25-vitamin D3 or dex in addition. There was no obvious correlation to osteocalcin (OCN) gene expression in these experiments. In MSCs from patients with metabolic bone disease (n = 9) UCN gene expression was significantly higher compared to MSCs from normal controls (n = 6). Human MSCs did not express any of the CRFR's during differentiation to osteoblasts. Our results indicate that UCN is produced during the development of MSCs to osteoblasts and differentially regulated during culture as well as by differentiation factors. The expression is maximal between proliferation and matrix maturation phase. However, UCN does not seem to act on the osteoblast itself as shown by the missing CRFR's. Our results suggest new perspectives on the role of urocortin in human skeletal tissue in health and disease."],["dc.identifier.doi","10.1007/s10735-009-9244-z"],["dc.identifier.isi","000275443300002"],["dc.identifier.pmid","19949969"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4160"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56279"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1567-2379"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Differentiation dependent expression of urocortin's mRNA and peptide in human osteoprogenitor cells: influence of BMP-2, TGF-beta-1 and dexamethasone"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","680"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Orthopaedic Research"],["dc.bibliographiccitation.lastpage","686"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Dumont, Clemens"],["dc.contributor.author","Kauer, Fritz"],["dc.contributor.author","Bohr, Stefan"],["dc.contributor.author","Schmidtmann, Ulrich"],["dc.contributor.author","Knopp, Werner"],["dc.contributor.author","Engelhardt, Thomas"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.contributor.author","Stuermer, Klaus Michael"],["dc.date.accessioned","2018-11-07T08:30:23Z"],["dc.date.available","2018-11-07T08:30:23Z"],["dc.date.issued","2009"],["dc.description.abstract","This article is about the evaluation of possible differences in biomechanical or histomorphological properties of bone healing between saw osteotomy and random fracturing after 6 months. A standardized, 30 oblique monocortical saw osteotomy of sheep tibia was carried out, followed by fracture completion of the opposed cortical bone. Fixation was performed by bridge plating (4.5 mm, LCDCP, broad). X-rays were taken immediately after surgery and at the end of the study. Polychrome fluorescent staining was performed according to a standardized protocol in the 2nd, 4th, 6th, 10th, 14th, 18th, 22th and 26th week. Ten sheep were comprehensively evaluated. Data For stiffness and histomorphology are reported. The average bending stiffness of the operated bone was higher (1.7 (SD 0.3) with plate (MP) vs. 1.5 without plate) than for the intact bone (1.4 (SD 0.2) though no significant differences in bending stiffness were observed (P > 0.05). Fluorescence staining revealed shows numbers of blood vessels and less fragment resorption and remodeling in the osteotomy gap. Bone healing after saw osteotomy shows a very close resemblance to 'normal' fracture healing. However, vascular density, fragment resorption, fragment remodeling, and callus remodeling are reduced at the osteotomy. (C) 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:680-686, 2009"],["dc.identifier.doi","10.1002/jor.20795"],["dc.identifier.isi","000265009900017"],["dc.identifier.pmid","18988260"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6273"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16887"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","John Wiley & Sons Inc"],["dc.relation.issn","0736-0266"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Long-Term Effects of Saw Osteotomy versus Random Fracturing on Bone Healing and Remodeling in a Sheep Tibia Model"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","851"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Phytotherapy Research"],["dc.bibliographiccitation.lastpage","858"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Sehmisch, Stefan"],["dc.contributor.author","Boeckhoff, J."],["dc.contributor.author","Wille, J."],["dc.contributor.author","Seidlova-Wuttke, Dana"],["dc.contributor.author","Rack, Thomas"],["dc.contributor.author","Tezval, Mohammed"],["dc.contributor.author","Wuttke, Wolfgang"],["dc.contributor.author","Stuermer, Klaus-Michael"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.date.accessioned","2018-11-07T08:29:35Z"],["dc.date.available","2018-11-07T08:29:35Z"],["dc.date.issued","2009"],["dc.description.abstract","Osteoporosis research undertaken in males is rare and there are only a few therapeutic options. Phytoestrogens might be a safe alternative for prophylaxis. Sixty 3-month-old male rats were orchidectomized and divided into five groups. The groups either received soy-free food (C), estradiol (E), testosterone (T) or Vitex agnus castus in different concentrations (AC high/AC low) for 12 weeks. The tibia metaphysis was tested biomechanically and histomorphometrically. The AC high group reached 87% of the biomechanical values of the estradiol group and was significantly superior to the control group. Testosterone supplementation resulted in poor biomechanical properties. The cortical bone parameters of the AC group were similar to the control group, while supplementation with estradiol and testosterone demonstrated a reduction of cortical bone. The AC high group reached 88.4% of trabecular bone area, 80.7% of trabecular number and 66.9% of the number of trabecular nodes compared with estradiol supplementation. Vitex agnus castus demonstrated osteoprotective effects in males. It preserves the cortical as well as the trabecular bone and might be a safe alternative for HRT. Testosterone supplementation has positive effects on trabecular bone, which are concurrently counteracted by the loss of cortical bone. Copyright (C) 2008 John Wiley & Sons, Ltd."],["dc.identifier.doi","10.1002/ptr.2711"],["dc.identifier.isi","000267088400018"],["dc.identifier.pmid","19107741"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16686"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","John Wiley & Sons Ltd"],["dc.relation.issn","0951-418X"],["dc.title","Vitex agnus castus as Prophylaxis for Osteopenia after Orchidectomy in Rats Compared with Estradiol and Testosterone Supplementation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","23"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Calcified Tissue International"],["dc.bibliographiccitation.lastpage","32"],["dc.bibliographiccitation.volume","86"],["dc.contributor.author","Kolios, Leila"],["dc.contributor.author","Hoerster, Ann Kristin"],["dc.contributor.author","Sehmisch, Stephan"],["dc.contributor.author","Malcherek, Marie Christin"],["dc.contributor.author","Rack, Thomas"],["dc.contributor.author","Tezval, Mohammed"],["dc.contributor.author","Seidlova-Wuttke, Dana"],["dc.contributor.author","Wuttke, Wolfgang"],["dc.contributor.author","Stuermer, Klaus Michael"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.date.accessioned","2018-11-07T08:47:15Z"],["dc.date.available","2018-11-07T08:47:15Z"],["dc.date.issued","2010"],["dc.description.abstract","Osteoporosis is accompanied by predominantly metaphyseal fractures with a delayed and qualitatively reduced healing process. This study addressed the question of whether fracture healing in the context of osteoporosis prophylaxis is improved with estrogen (E) or alendronate (ALN). Thirty-six ovariectomized and 12 sham-operated 12-week-old rats received soy-free (osteoporotic C, sham), E-, or ALN- supplemented diets. After 10 weeks, a metaphyseal tibia osteotomy and standardized T-plate fixation were performed. After a 5-week healing process, the fracture callus was evaluated qualitatively by biomechanical bending test and quantitatively in microradiographic sections. The time course of callus formation was examined using fluorochrome-labeled histological sections. Administration of E improved the biomechanical properties of callus (stiffness [N/mm]: sham: 110.2 +/- A 76.07, C: 41.28 +/- A 33.70, E: 85.72 +/- A 47.24, ALN: 72.07 +/- A 34.68). The resistance to microfracturing seen in E-treated animals was significantly enhanced and even superior to sham (yield load [N] sham: 27.44 +/- A 9.72, C: 21.04 +/- A 12.47, E: 42.85 +/- A 13.74(a dagger), ALN: 25.28 +/- A 6.4(center dot)) ( P < 0.05 vs. sham group, (a dagger) P < 0.05 vs. C group, (aEuro cent) P < 0.05 vs. E group). Trabecular bone in particular was improved, indicating the presence of physiological endosteal bridging (Tr.Dn [%] sham: 10.53 +/- A 18.9, C: 1.01 +/- A 0.14, E: 24.13 +/- A 34.09(a dagger), ALN: 3.99 +/- A 8.3(center dot)). ALN did not help bone healing, as shown by mechanical tests. Compared to the C group, statistically, ALN did not show worse properties. The induction of callus formation under ALN treatment was slightly delayed (Tt.Cl [mm(2)] sham: 3.68 +/- A 0.66, C: 3.44 +/- A 0.42, E: 3.69 +/- A 0.58, ALN: 3.06 +/- A 0.56). Osteoporotic metaphyseal fracture healing was qualitatively and quantitatively improved by E prophylaxis. The process of fracture healing occurred nearly physiologically (shamlike). Notably, ALN hardly improved metaphyseal callus properties when assessed as osteoporosis prophylaxis, but to a lesser extent than E."],["dc.description.sponsorship","DFG [STU 478/2-1]"],["dc.identifier.doi","10.1007/s00223-009-9318-7"],["dc.identifier.isi","000273102700004"],["dc.identifier.pmid","19949941"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4027"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20901"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0171-967X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Do Estrogen and Alendronate Improve Metaphyseal Fracture Healing When Applied as Osteoporosis Prophylaxis?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","529"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","World Journal of Urology"],["dc.bibliographiccitation.lastpage","534"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Tezval, Mohammed"],["dc.contributor.author","Serferaz, G."],["dc.contributor.author","Rack, Thomas"],["dc.contributor.author","Kolios, Leila"],["dc.contributor.author","Sehmisch, Stefan"],["dc.contributor.author","Schmelz, Ulrich"],["dc.contributor.author","Tezval, Hossein"],["dc.contributor.author","Stuermer, Klaus-Michael"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.date.accessioned","2018-11-07T08:53:48Z"],["dc.date.available","2018-11-07T08:53:48Z"],["dc.date.issued","2011"],["dc.description.abstract","Management of hypogonadism-induced osteoporosis in elderly men is still a challenge. We investigated the short-term effects of parathyroid hormone (PTH) treatments on strength, micro-architecture, and mineral density of trochanteric region of orchiectomized rat femur. Eight-month-old male Sprague-Dawley rats (n = 44) were divided into two groups: (1) orchiectomized (ORX) and (2) sham group. Twelve weeks after orchiectomy, half of the orchiectomized animals were treated with daily subcutaneously injected PTH (0.040 mg/kg/BW) (ORX-PTH) for 5 weeks. The other half remained untreated (ORX). The sham-operated group was divided and treated in the same way (sham, sham-PTH). After 5 weeks, both femurs were excised for biomechanical and histomorphometric analysis, trabecular measurements, mineral content assessment, and immunofluorescence analysis. The femoral trochanteric strength after PTH treatment was enhanced in the breaking test (ORX-F-max = 158.7 N vs. ORX + PTH-F-max = 202 N). Stiffness of treated ORX animals reached nearly the levels observed in untreated sham rats. PTH therapy improved the trabecular connectivity, width, and area (ORX-Tb.Ar = 47.79% vs. ORX + PTH-Tb.Ar = 68.47%, P < 0.05) in the proximal femur. The treated rats showed significantly improved mineral content in ashed femurs (ORX-mineral content = 43.73% vs. ORX + PTH-mineral content = 49.49%) when compared to the untreated animals. A comparison of widths of fluorescence bands in cortical bone of the subtrochanteric cross-sections showed a significant increase in oppositions after the PTH therapy. Our finding supports the hypothesis that PTH therapy seems to be a rational therapy in patients with hypogonadism induced bone loss and improves the bone strength of trochanteric region of rat femur."],["dc.identifier.doi","10.1007/s00345-011-0652-9"],["dc.identifier.isi","000293136200019"],["dc.identifier.pmid","21298272"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7116"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22511"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1433-8726"],["dc.relation.issn","0724-4983"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Effect of parathyroid hormone on hypogonadism induced bone loss of proximal femur of orchiectomized rat"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","251"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Osteoporosis International"],["dc.bibliographiccitation.lastpage","261"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Tezval, Mohammed"],["dc.contributor.author","Stuermer, Ewa Klara"],["dc.contributor.author","Sehmisch, Stefan"],["dc.contributor.author","Rack, Thomas"],["dc.contributor.author","Stary, A."],["dc.contributor.author","Stebener, M."],["dc.contributor.author","Konietschke, Frank"],["dc.contributor.author","Stuermer, Klaus-Michael"],["dc.date.accessioned","2018-11-07T08:46:31Z"],["dc.date.available","2018-11-07T08:46:31Z"],["dc.date.issued","2010"],["dc.description.abstract","We have examined the changes induced in the trochanteric region of femur of ovariectomized rat after administration of estradiol and p.arathyroid hormone. We have developed a reproducible biomechanical test and produced trochanteric fractures to evaluate stiffness and strength of this region in addition to histomorphometry. We investigated the short-term effects of parathyroid hormone (PTH) and estrogen (E) on the strength of the rat trochanteric region in a new mechanical test. Forty-four 3-month-old female Sprague-Dawley rats were ovariectomized and 8 weeks later treated with soy-free diet (C), daily applications of orally supplied E (0.5 mg/kg food) or subcutaneously injected PTH (0.014 mg/kg), for 5 weeks, and an additional untreated group was added as sham-operated. The femurs were examined for biomechanical and histomorphometric changes. Our new mechanical test was validated in a right-left comparison. The PTH treatment induced significantly superior biomechanical results (F (max) = 225.3 N, stiffness = 314.9 N/mm) compared to E (F (max) = 182.9 N, stiffness = 237.2 N/mm), C (F (max) = 166.03 N, stiffness = 235.56 N/mm), and sham (F (max) = 192.1 N, stiffness = 267.2 N/mm). Animals of the PTH group demonstrated a significantly improved trabecular bone structure and area (75.67%) in comparison to the E (61.04%) and C (57.18%) groups. Our new biomechanical test is valid and produces trochanteric fracture. Our results show that the short-term antiosteoporotic effects of PTH are in the trochanteric region of ovariectomized rat superior to E."],["dc.identifier.doi","10.1007/s00198-009-0941-y"],["dc.identifier.isi","000273327000006"],["dc.identifier.pmid","19436940"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4023"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20712"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","London"],["dc.relation.issn","0937-941X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Improvement of trochanteric bone quality in an osteoporosis model after short-term treatment with parathyroid hormone: a new mechanical test for trochanteric region of rat femur"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]