Duque-Afonso, Carlos J.

[["dc.bibliographiccitation.firstpage","10226"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Nanoscale"],["dc.bibliographiccitation.lastpage","10239"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Schlichthaerle, Thomas"],["dc.contributor.author","Duque-Afonso, Carlos J."],["dc.contributor.author","Alevra, Mihai"],["dc.contributor.author","Strauss, Sebastian"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Jungmann, Ralf"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2021-04-14T08:26:44Z"],["dc.date.available","2021-04-14T08:26:44Z"],["dc.date.issued","2020"],["dc.description.abstract","A standard procedure to study cellular elements is via immunostaining followed by optical imaging. This methodology typically requires target-specific primary antibodies (1.Abs), which are revealed by secondary antibodies (2.Abs). Unfortunately, the antibody bivalency, polyclonality, and large size can result in a series of artifacts. Alternatively, small, monovalent probes, such as single-domain antibodies (nanobodies) have been suggested to minimize these limitations. The discovery and validation of nanobodies against specific targets are challenging, thus only a minimal amount of them are currently available. Here, we used STED, DNA-PAINT, and light-sheet microscopy, to demonstrate that secondary nanobodies (1) increase localization accuracy compared to 2.Abs; (2) allow direct pre-mixing with 1.Abs before staining, reducing experimental time, and enabling the use of multiple 1.Abs from the same species; (3) penetrate thick tissues more efficiently; and (4) avoid probe-induced clustering of target molecules observed with conventional 2.Abs in living or poorly fixed samples. Altogether, we show how secondary nanobodies are a valuable alternative to 2.Abs."],["dc.identifier.doi","10.1039/d0nr00227e"],["dc.identifier.pmid","32356544"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82054"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/185"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2040-3372"],["dc.relation.haserratum","/handle/2/81970"],["dc.relation.issn","2040-3364"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 3.0"],["dc.title","Circumvention of common labelling artefacts using secondary nanobodies"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","24543"],["dc.bibliographiccitation.issue","48"],["dc.bibliographiccitation.journal","Nanoscale"],["dc.bibliographiccitation.lastpage","24543"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Schlichthaerle, Thomas"],["dc.contributor.author","Duque-Afonso, Carlos J."],["dc.contributor.author","Alevra, Mihai"],["dc.contributor.author","Strauss, Sebastian"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Jungmann, Ralf"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2021-04-14T08:26:30Z"],["dc.date.available","2021-04-14T08:26:30Z"],["dc.date.issued","2020"],["dc.description.abstract","Correction for ‘Circumvention of common labelling artefacts using secondary nanobodies’ by Shama Sograte-Idrissi et al., Nanoscale, 2020, 12, 10226–10239, DOI: 10.1039/D0NR00227E."],["dc.identifier.doi","10.1039/d0nr90279a"],["dc.identifier.pmid","33306074"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81970"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/419"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2040-3372"],["dc.relation.iserratumof","/handle/2/82054"],["dc.relation.issn","2040-3364"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 3.0"],["dc.title","Correction: Circumvention of common labelling artefacts using secondary nanobodies"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","erratum_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]