Zimmermann, Ortrud

[["dc.bibliographiccitation.artnumber","172"],["dc.bibliographiccitation.journal","BMC Pregnancy and Childbirth"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Voelker, Fabian M."],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Uy, Angela"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Gross, Uwe"],["dc.date.accessioned","2018-11-07T10:22:50Z"],["dc.date.available","2018-11-07T10:22:50Z"],["dc.date.issued","2017"],["dc.description.abstract","Background: Although infectious diseases still account for a high burden of morbidity and mortality in sub-Saharan Africa, simultaneous investigations on multiple infections affecting maternal and child health are missing. Methods: We conducted a cross-sectional, single-centre pilot study in a rural area of Ghana to assess the infectiological profile during pregnancy. Screening of 180 expectant mothers was done by vaginal swabs and serology to detect the most common pregnancy-relevant infections. They were also interviewed for potential risk factors, outcome of previous pregnancies, and socio-economic aspects. Results: We found a high prevalence of infections caused by hepatitis B virus (16.7% HBs antigen positive). In contrast, infections caused by hepatitis C virus (1.1% anti-HCV) and HIV (0.6%) were rare. Maternal malaria was frequent (10.6%), despite increasing acceptance of intermittent preventive treatment during pregnancy (IPTp). Group B streptococci were present in 10.6% of all pregnant women. Absence of antibodies against varicella zoster virus in 43.2%, Toxoplasma gondii in 26.8%, parvovirus B19 in 20.0%, and rubella virus in 15.7% makes a significant proportion of pregnant women susceptible for acquiring primary infections. Whereas all study participants had specific IgG antibodies against human cytomegalovirus, infections with Listeria, Brucella, or Neisseria gonorrhoeae as well as active syphilis were absent. Conclusions: Our pilot study in a rural community in Ghana indicates an urgent need for action in dealing at least with high-prevalent pregnancy-relevant infections, such as hepatitis B, malaria and those caused by group B streptococci. In addition, the resulting prevalence rates of various other infections may offer guidance for health officials to prioritize possible future intervention schemes."],["dc.identifier.doi","10.1186/s12884-017-1351-3"],["dc.identifier.isi","000402791800001"],["dc.identifier.pmid","28583150"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14507"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42347"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2393"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Prevalence of pregnancy-relevant infections in a rural setting of Ghana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2087"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Emele, Matthias F."],["dc.contributor.author","Joppe, Felix M."],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Rupnik, Maja"],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Kusumawati, R. Lia"],["dc.contributor.author","Berger, Fabian K."],["dc.contributor.author","Laukien, Friederike"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Zautner, Andreas E."],["dc.date.accessioned","2019-09-24T08:07:22Z"],["dc.date.available","2019-09-24T08:07:22Z"],["dc.date.issued","2019"],["dc.description.abstract","Clostridioides difficile, a Gram-positive spore-forming bacterium, is the leading cause of nosocomial diarrhea worldwide and therefore a substantial burden to the healthcare system. During the past decade, hypervirulent PCR-ribotypes (RT) e.g., RT027 or RT176 emerged rapidly all over the world, associated with both, increased severity and mortality rates. It is thus of great importance to identify epidemic strains such as RT027 and RT176 as fast as possible. While commonly used diagnostic methods, e.g., multilocus sequence typing (MLST) or PCR-ribotyping, are time-consuming, proteotyping offers a fast, inexpensive, and reliable alternative solution. In this study, we established a MALDI-TOF-based typing scheme for C. difficile. A total of 109 ribotyped strains representative for five MLST clades were analyzed by MALDI-TOF. MLST, based on whole genome sequences, and PCR-ribotyping were used as reference methods. Isoforms of MS-detectable biomarkers, typically ribosomal proteins, were related with the deduced amino acid sequences and added to the C. difficile proteotyping scheme. In total, we were able to associate nine biomarkers with their encoding genes and include them in our proteotyping scheme. The discriminatory capacity of the C. difficile proteotyping scheme was mainly based on isoforms of L28-M (2 main isoforms), L35-M (4 main isoforms), and S20-M (2 main isoforms) giving rise to at least 16 proteotyping-derived types. In our test population, five of these 16 proteotyping-derived types were detected. These five proteotyping-derived types did not correspond exactly to the included five MLST-based C. difficile clades, nevertheless the subtyping depth of both methods was equivalent. Most importantly, proteotyping-derived clade B contained only isolates of the hypervirulent RT027 and RT176. Proteotyping is a stable and easy-to-perform intraspecies typing method and a promising alternative to currently used molecular techniques. It is possible to distinguish the group of RT027 and RT176 isolates from non-RT027/non-RT176 isolates using proteotyping, providing a valuable diagnostic tool."],["dc.identifier.doi","10.3389/fmicb.2019.02087"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16398"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62451"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Proteotyping of Clostridioides difficile as Alternate Typing Method to Ribotyping Is Able to Distinguish the Ribotypes RT027 and RT176 From Other Ribotypes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1843"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Seugendo, Mwanaisha"],["dc.contributor.author","Janssen, Iryna"],["dc.contributor.author","Lang, Vanessa"],["dc.contributor.author","Hasibuan, Irene"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Kusumawati, R. L."],["dc.contributor.author","Mshana, Stephen E."],["dc.contributor.author","von Müller, Lutz"],["dc.contributor.author","Okamo, Benard"],["dc.contributor.author","Ortlepp, Jan R."],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Rupnik, Maja"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Groß, Uwe"],["dc.date.accessioned","2019-07-09T11:45:47Z"],["dc.date.available","2019-07-09T11:45:47Z"],["dc.date.issued","2018"],["dc.description.abstract","Clostridioides (Clostridium) difficile infections (CDI) are considered worldwide as emerging health threat. Uptake of C. difficile spores may result in asymptomatic carrier status or lead to CDI that could range from mild diarrhea, eventually developing into pseudomembranous colitis up to a toxic megacolon that often results in high mortality. Most epidemiological studies to date have been performed in middle- and high income countries. Beside others, the use of antibiotics and the composition of the microbiome have been identified as major risk factors for the development of CDI. We therefore postulate that prevalence rates of CDI and the distribution of C. difficile strains differ between geographical regions depending on the regional use of antibiotics and food habits. A total of 593 healthy control individuals and 608 patients suffering from diarrhea in communities in Germany, Ghana, Tanzania and Indonesia were selected for a comparative multi-center cross-sectional study. The study populations were screened for the presence of C. difficile in stool samples. Cultured C. difficile strains (n = 84) were further subtyped and characterized using PCR-ribotyping, determination of toxin production, and antibiotic susceptibility testing. Prevalence rates of C. difficile varied widely between the countries. Whereas high prevalence rates were observed in symptomatic patients living in Germany and Indonesia (24.0 and 14.7%), patients from Ghana and Tanzania showed low detection rates (4.5 and 6.4%). Differences were also obvious for ribotype distribution and toxin repertoires. Toxin A+/B+ ribotypes 001/072 and 078 predominated in Germany, whereas most strains isolated from Indonesian patients belonged to toxin A+/B+ ribotype SLO160 and toxin A-/B+ ribotype 017. With 42.9-73.3%, non-toxigenic strains were most abundant in Africa, but were also found in Indonesia at a rate of 18.2%. All isolates were susceptible to vancomycin and metronidazole. Mirroring the antibiotic use, however, moxifloxacin resistance was absent in African C. difficile isolates but present in Indonesian (24.2%) and German ones (65.5%). This study showed that CDI is a global health threat with geographically different prevalence rates which might reflect distinct use of antibiotics. Significant differences for distributions of ribotypes, toxin production, and antibiotic susceptibilities were observed."],["dc.identifier.doi","10.3389/fmicb.2018.01843"],["dc.identifier.pmid","30131799"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15318"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59311"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-302X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Prevalence and Strain Characterization of Clostridioides (Clostridium) difficile in Representative Regions of Germany, Ghana, Tanzania and Indonesia - A Comparative Multi-Center Cross-Sectional Study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","652"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","656"],["dc.bibliographiccitation.volume","306"],["dc.contributor.author","Janssen, Iryna"],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Rupnik, Maja"],["dc.contributor.author","Wetzel, Daniela"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Gross, Uwe"],["dc.date.accessioned","2018-11-07T10:05:02Z"],["dc.date.available","2018-11-07T10:05:02Z"],["dc.date.issued","2016"],["dc.description.abstract","Since data about Clostridium difficile infection in sub-Saharan Africa are scarce, we determined its epidemiology and risk factors in a cross-sectional study in Eikwe, a rural community in Ghana. We tested stool samples from 176 hospitalized patients with diarrhoea and from 131 asymptomatic non-hospitalized individuals for C difficile and some other enteric pathogens. The overall prevalence rate of C difficile was 4.9% with ribotype 084 being predominant. With 75% of the isolates, a high rate of nontoxigenic strains was present in symptomatic patients, most of whom had no other identified enteric pathogens. All strains were susceptible against metronidazole and vancomycin, respectively. Data on lifestyle and medical history showed that age <5 years (p=0.004), and use of ceftriaxone (p =0.023) were the most important risk factors for C difficile carriage status. Although our data suggest that C. difficile is currently not a major cause of diarrhoea in this setting, the epidemiology of C difficile in sub-Saharan Africa awaits further investigation. (C) 2016 Elsevier GmbH. All rights reserved."],["dc.description.sponsorship","Federal State of Lower Saxony, Niedersachsisches Vorab [VWZN2889]"],["dc.identifier.doi","10.1016/j.ijmm.2016.09.004"],["dc.identifier.isi","000390824600007"],["dc.identifier.pmid","27693000"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38815"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.relation.issn","1618-0607"],["dc.relation.issn","1438-4221"],["dc.title","High prevalence of nontoxigenic Clostridium difficile isolated from hospitalized and non-hospitalized individuals in rural Ghana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]