Töpperwien, Mareike

[["dc.bibliographiccitation.firstpage","368"],["dc.bibliographiccitation.journal","Talanta"],["dc.bibliographiccitation.lastpage","376"],["dc.bibliographiccitation.volume","161"],["dc.contributor.author","Surowka, A.D."],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Bernhardt, Markus"],["dc.contributor.author","Nicolas, J. D."],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Adamek, D."],["dc.contributor.author","Szczerbowska-Boruchowska, M."],["dc.date.accessioned","2020-03-11T09:18:44Z"],["dc.date.available","2020-03-11T09:18:44Z"],["dc.date.issued","2016"],["dc.description.abstract","Human dopaminergic system in general, and substantia nigra (SN) neurons, in particular, are implicated in the pathologies underlying the human brain aging. The interplay between aberrations in the structural organization and elemental composition of SN neuron bodies has recently gained in importance as selected metals: Fe, Cu, Zn, Ca were found to trigger oxidative-stress-mediated aberration in their molecular assembly due to concomitant protein (alpha-synuclein, tau-protein) aggregation, gliosis and finally oxidative stress. In the present study, we demonstrate an integrated approach to the analysis of the structural organization, assembly, and metals' accumulation in two distinct areas of SN: in the neuromelanin neurons and neuropil. By using the highly brilliant source of PETRA III and the Kirkpatrick-Baez nano-focus, large area histological brain slices are scanned at the sub-neuronal resolution, taking advantage of continuous motor movement and reduced acquisition time. Elemental analysis with synchrotron radiation based X-ray Fluorescence (SRXRF) is combined with X-ray Phase Contrast Imaging (XPCI) to correct for inherent aberrations in the samples' density and thickness, often referred to as the mass thickness effect. Based on the raw SRXRF spectra, we observed the accumulation of P, S, Cl, K, Ca, Fe, Cu and Zn predominantly in the SN neurons. However, upon the mass thickness correction, the distributions of Cl became significantly more uniform. Simultaneously with the fluorescence signal, the Small Angle X-ray Scattering (SAXS) is recorded by a pixel detector positioned in the far-field, enabling fast online computation of the darkfield and differential phase contrast (DPC). The data has demonstrated the SN neurons and neuropil produces excellent contrast which is due to their different mass density and scattering strength, indicative of differences in local structure and assembly therein. In all, the results show that combined SRXRF-XPCI-SAXS experiments can robustly serve as a unique tool for understanding the interplay between the chemical composition and structural organization that may drive the biochemical age-related processes occurring in the human dopaminergic system."],["dc.identifier.doi","10.1016/j.talanta.2016.08.023"],["dc.identifier.gro","3142481"],["dc.identifier.pmid","27769419"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63294"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.eissn","1873-3573"],["dc.relation.issn","0039-9140"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.title","Combined in-situ imaging of structural organization and elemental composition of substantia nigra neurons in the elderly"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","518"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","529"],["dc.bibliographiccitation.volume","28"],["dc.contributor.affiliation","Wittmeier, Andrew; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Cassini, Chiara; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Töpperwien, Mareike; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Denz, Manuela; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Hagemann, Johannes; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Osterhoff, Markus; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Salditt, Tim; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.author","Wittmeier, Andrew"],["dc.contributor.author","Cassini, Chiara"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Denz, Manuela"],["dc.contributor.author","Hagemann, Johannes"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2021-04-14T08:28:23Z"],["dc.date.available","2021-04-14T08:28:23Z"],["dc.date.issued","2021"],["dc.date.updated","2022-02-09T13:21:31Z"],["dc.description.abstract","X‐rays are emerging as a complementary probe to visible‐light photons and electrons for imaging biological cells. By exploiting their small wavelength and high penetration depth, it is possible to image whole, intact cells and resolve subcellular structures at nanometer resolution. A variety of X‐ray methods for cell imaging have been devised for probing different properties of biological matter, opening up various opportunities for fully exploiting different views of the same sample. Here, a combined approach is employed to study cell nuclei of NIH‐3T3 fibroblasts. Scanning small‐angle X‐ray scattering is combined with X‐ray holography to quantify length scales, aggregation state, and projected electron and mass densities of the nuclear material. Only by joining all this information is it possible to spatially localize nucleoli, heterochromatin and euchromatin, and physically characterize them. It is thus shown that for complex biological systems, like the cell nucleus, combined imaging approaches are highly valuable."],["dc.description.abstract","The combination of small‐angle X‐ray scattering and X‐ray holography enables us to visualize and characterize biological material in cell nuclei spanning multiple length scales. image"],["dc.identifier.doi","10.1107/S1600577520016276"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82591"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/218"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","International Union of Crystallography"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1600-5775"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use,\r\n distribution and reproduction in any medium, provided the original work is properly cited."],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cellular biophysics"],["dc.title","Combined scanning small-angle X-ray scattering and holography probes multiple length scales in cell nuclei"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]