Now showing 1 - 10 of 12
  • 2018Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","24"],["dc.bibliographiccitation.issue","S2"],["dc.bibliographiccitation.journal","Microscopy and Microanalysis"],["dc.bibliographiccitation.lastpage","25"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Robisch, Anna Lena"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-03-04T13:40:15Z"],["dc.date.available","2020-03-04T13:40:15Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1017/S1431927618012540"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63109"],["dc.language.iso","en"],["dc.relation.issn","1431-9276"],["dc.relation.issn","1435-8115"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","3d Virtual Histology of Human Cerebellum by Propagation-Based X-Ray Phase-Contrast Tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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  • 2018Journal Article Research Paper
    [["dc.bibliographiccitation.artnumber","041109"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Applied Physics Letters"],["dc.bibliographiccitation.volume","113"],["dc.contributor.author","Hagemann, Johannes"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-04-23T14:35:46Z"],["dc.date.available","2020-04-23T14:35:46Z"],["dc.date.issued","2018"],["dc.description.abstract","X-ray phase contrast imaging based on free space propagation relies on phase retrieval to obtain sharp images of micro- and nanoscale objects, with widespread applications in material science and biomedical research. For high resolution synchrotron experiments, phase retrieval is largely based on the single step reconstruction using the contrast transfer function approach (CTF), as introduced almost twenty years ago [Cloetens et al., Appl. Phys. Lett. 75, 2912 (1999)]. Notwithstanding its tremendous merits, this scheme makes stringent assumptions on the optical properties of the object, requiring, in particular, a weakly varying phase. In this work, we show how significant the loss in image quality becomes if these assumption are violated, and how phase retrieval can be easily improved by a simple scheme of alternating projections. Importantly, the approach demonstrated here uses the same input data and constraint sets as the conventional CTF-based phase retrieval, and is particularly well suited for the holographic regime."],["dc.identifier.doi","10.1063/1.5029927"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/64332"],["dc.language.iso","en"],["dc.relation.eissn","1077-3118"],["dc.relation.issn","0003-6951"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.title","Phase retrieval for near-field X-ray imaging beyond linearisation or compact support"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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  • 2021-05-04Journal Article Research Paper
    [["dc.bibliographiccitation.artnumber","e2014472118"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Keppeler, Daniel"],["dc.contributor.author","Kampshoff, Christoph A."],["dc.contributor.author","Thirumalai, Anupriya"],["dc.contributor.author","Duque-Afonso, Carlos J."],["dc.contributor.author","Schaeper, Jannis J."],["dc.contributor.author","Quilitz, Tabea"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Vogl, Christian"],["dc.contributor.author","Hessler, Roland"],["dc.contributor.author","Meyer, Alexander"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2021-06-02T14:33:54Z"],["dc.date.available","2021-06-02T14:33:54Z"],["dc.date.issued","2021-05-04"],["dc.description.abstract","The cochlea of our auditory system is an intricate structure deeply embedded in the temporal bone. Compared with other sensory organs such as the eye, the cochlea has remained poorly accessible for investigation, for example, by imaging. This limitation also concerns the further development of technology for restoring hearing in the case of cochlear dysfunction, which requires quantitative information on spatial dimensions and the sensorineural status of the cochlea. Here, we employed X-ray phase-contrast tomography and light-sheet fluorescence microscopy and their combination for multiscale and multimodal imaging of cochlear morphology in species that serve as established animal models for auditory research. We provide a systematic reference for morphological parameters relevant for cochlear implant development for rodent and nonhuman primate models. We simulate the spread of light from the emitters of the optical implants within the reconstructed nonhuman primate cochlea, which indicates a spatially narrow optogenetic excitation of spiral ganglion neurons."],["dc.identifier.doi","10.1073/pnas.2014472118"],["dc.identifier.pmid","33903231"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87093"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/251"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1091-6490"],["dc.relation.issn","0027-8424"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Multiscale photonic imaging of the native and implanted cochlea"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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  • 2016Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","368"],["dc.bibliographiccitation.journal","Talanta"],["dc.bibliographiccitation.lastpage","376"],["dc.bibliographiccitation.volume","161"],["dc.contributor.author","Surowka, A.D."],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Bernhardt, Markus"],["dc.contributor.author","Nicolas, J. D."],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Adamek, D."],["dc.contributor.author","Szczerbowska-Boruchowska, M."],["dc.date.accessioned","2020-03-11T09:18:44Z"],["dc.date.available","2020-03-11T09:18:44Z"],["dc.date.issued","2016"],["dc.description.abstract","Human dopaminergic system in general, and substantia nigra (SN) neurons, in particular, are implicated in the pathologies underlying the human brain aging. The interplay between aberrations in the structural organization and elemental composition of SN neuron bodies has recently gained in importance as selected metals: Fe, Cu, Zn, Ca were found to trigger oxidative-stress-mediated aberration in their molecular assembly due to concomitant protein (alpha-synuclein, tau-protein) aggregation, gliosis and finally oxidative stress. In the present study, we demonstrate an integrated approach to the analysis of the structural organization, assembly, and metals' accumulation in two distinct areas of SN: in the neuromelanin neurons and neuropil. By using the highly brilliant source of PETRA III and the Kirkpatrick-Baez nano-focus, large area histological brain slices are scanned at the sub-neuronal resolution, taking advantage of continuous motor movement and reduced acquisition time. Elemental analysis with synchrotron radiation based X-ray Fluorescence (SRXRF) is combined with X-ray Phase Contrast Imaging (XPCI) to correct for inherent aberrations in the samples' density and thickness, often referred to as the mass thickness effect. Based on the raw SRXRF spectra, we observed the accumulation of P, S, Cl, K, Ca, Fe, Cu and Zn predominantly in the SN neurons. However, upon the mass thickness correction, the distributions of Cl became significantly more uniform. Simultaneously with the fluorescence signal, the Small Angle X-ray Scattering (SAXS) is recorded by a pixel detector positioned in the far-field, enabling fast online computation of the darkfield and differential phase contrast (DPC). The data has demonstrated the SN neurons and neuropil produces excellent contrast which is due to their different mass density and scattering strength, indicative of differences in local structure and assembly therein. In all, the results show that combined SRXRF-XPCI-SAXS experiments can robustly serve as a unique tool for understanding the interplay between the chemical composition and structural organization that may drive the biochemical age-related processes occurring in the human dopaminergic system."],["dc.identifier.doi","10.1016/j.talanta.2016.08.023"],["dc.identifier.gro","3142481"],["dc.identifier.pmid","27769419"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63294"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.eissn","1873-3573"],["dc.relation.issn","0039-9140"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.title","Combined in-situ imaging of structural organization and elemental composition of substantia nigra neurons in the elderly"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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  • 2016Conference Paper
    [["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Quade, Felix"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Khounsary, Ali M."],["dc.contributor.editor","Dorssen, Gert E. van"],["dc.date.accessioned","2017-09-07T11:54:05Z"],["dc.date.available","2017-09-07T11:54:05Z"],["dc.date.issued","2016"],["dc.description.abstract","Due to the large penetration depth and small wavelength hard x-rays offer a unique potential for 3D biomedical and biological imaging, combining capabilities of high resolution and large sample volume. However, in classical absorption-based computed tomography, soft tissue only shows a weak contrast, limiting the actual resolution. With the advent of phase-contrast methods, the much stronger phase shift induced by the sample can now be exploited. For high resolution, free space propagation behind the sample is particularly well suited to make the phase shift visible. Contrast formation is based on the self-interference of the transmitted beam, resulting in object-induced intensity modulations in the detector plane. As this method requires a sufficiently high degree of spatial coherence, it was since long perceived as a synchrotron-based imaging technique. In this contribution we show that by combination of high brightness liquid-metal jet microfocus sources and suitable sample preparation techniques, as well as optimized geometry, detection and phase retrieval, excellent three-dimensional image quality can be obtained, revealing the anatomy of a cobweb spider in high detail. This opens up new opportunities for 3D virtual histology of small organisms. Importantly, the image quality is finally augmented to a level accessible to automatic 3D segmentation."],["dc.identifier.doi","10.1117/12.2246460"],["dc.identifier.gro","3145109"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2809"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","SPIE"],["dc.publisher.place","Bellingham, Washington"],["dc.relation.conference","Advances in Laboratory-Based X-Ray Sources, Optics, and Applications"],["dc.relation.eventend","2016-08-31"],["dc.relation.eventlocation","San Diego, Calif."],["dc.relation.eventstart","2016-08-30"],["dc.relation.ispartof","Advances in Laboratory-Based X-Ray Sources, Optics, and Applications V"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Laboratory-based x-ray phase-contrast tomography enables 3D virtual histology"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]
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  • 2017Conference Paper
    [["dc.bibliographiccitation.firstpage","4"],["dc.contributor.author","Nicolas, J. D."],["dc.contributor.author","Markus, Marietta Andrea"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Wang, Geng"],["dc.contributor.editor","Müller-Myhsok, Bertram"],["dc.date.accessioned","2020-06-26T10:11:47Z"],["dc.date.available","2020-06-26T10:11:47Z"],["dc.date.issued","2017"],["dc.description.abstract","In this work we present x-ray phase-contrast tomography of heart tissue from mouse, combining computed tomography (CT) scans with laboratory and synchrotron radiation. The work serves as a proof-of-concept that the cyto-architecture and in particular the myofibril orientation can be assessed in three dimensions (3D) by phase-contrast CT. We demonstrate the synergistic use of laboratory μ -CT and of the high resolution synchrotron setup based on waveguide optics. Details on preparation, instrumentation and analysis are given, as a state of the art reference for heart tissue tomography, and as a starting point for further progress."],["dc.identifier.doi","10.1117/12.2276648"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66750"],["dc.language.iso","en"],["dc.notes.preprint","yes"],["dc.relation.eventend","2017-09-25"],["dc.relation.eventlocation","San Diego"],["dc.relation.eventstart","2017-09-25"],["dc.relation.isbn","9781510612396"],["dc.relation.isbn","9781510612402"],["dc.relation.iserratumof","yes"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Nanoscale holographic tomography of heart tissue with x-ray waveguide optics"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]
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  • 2017Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","282"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Acta Crystallographica Section A Foundations and Advances"],["dc.bibliographiccitation.lastpage","292"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Toepperwien, Mareike"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2022-03-01T11:47:06Z"],["dc.date.available","2022-03-01T11:47:06Z"],["dc.date.issued","2017"],["dc.description.abstract","X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data."],["dc.description.abstract","X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data."],["dc.identifier.doi","10.1107/S2053273317007902"],["dc.identifier.doi","10.1107/s2053273317007902"],["dc.identifier.gro","3142469"],["dc.identifier.pii","S2053273317007902"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103911"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.notes.status","final"],["dc.relation.eissn","2053-2733"],["dc.relation.issn","2053-2733"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights.uri","http://creativecommons.org/licenses/by/2.0/uk/legalcode"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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  • 2016Conference Paper
    [["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Müller, Kristin"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Stock, Stuart R."],["dc.contributor.editor","Müller, Bert"],["dc.contributor.editor","Wang, Ge"],["dc.date.accessioned","2017-09-07T11:54:06Z"],["dc.date.available","2017-09-07T11:54:06Z"],["dc.date.issued","2016"],["dc.description.abstract","Assessing the three-dimensional architecture of neuronal tissues with sub-cellular resolution presents a significant analytical challenge. Overcoming the limitations associated with serial slicing, phase-contrast x-ray tomography has the potential to contribute to this goal. Even compact laboratory CT at an optimized liquid-metal jet micro- focus source combined with suitable phase-retrieval algorithms and preparation protocols can yield renderings with single cell sensitivity in millimeter sized brain areas of mouse. Here, we show the capabilities of the setup by imaging a Golgi-Cox impregnated mouse brain. Towards higher resolution we extend these studies at our recently upgraded waveguide-based cone-beam holo-tomography instrument GINIX at DESY. This setup allows high resolution recordings with adjustable field of view and resolution, down to the voxel sizes in the range of a few ten nanometers. The recent results make us confident that important issues of neuronal connectivity can be addressed by these methods, and that 3D (virtual) histology with nanoscale resolution will become an attractive modality for neuroscience research."],["dc.identifier.doi","10.1117/12.2238496"],["dc.identifier.gro","3145108"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2808"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","SPIE-Intl Soc Optical Eng"],["dc.publisher.place","Bellingham, Washington"],["dc.relation.conference","Developments in X-Ray Tomography X"],["dc.relation.eventend","2016-08-31"],["dc.relation.eventlocation","San Diego, Calif."],["dc.relation.eventstart","2016-08-29"],["dc.relation.isbn","978-1-5106-0325-7"],["dc.relation.ispartof","Developments in X-Ray Tomography X"],["dc.relation.issn","0277-786X"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Phase-contrast tomography of neuronal tissues: from laboratory- to high resolution synchrotron CT"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]
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  • 2018Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","40"],["dc.bibliographiccitation.issue","S2"],["dc.bibliographiccitation.journal","Microscopy and Microanalysis"],["dc.bibliographiccitation.lastpage","41"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Hagemann, Johannes"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-03-04T13:38:24Z"],["dc.date.available","2020-03-04T13:38:24Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1017/S143192761801262X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63108"],["dc.language.iso","en"],["dc.relation.issn","1431-9276"],["dc.relation.issn","1435-8115"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","Solving the Phase Problem in X-Ray Near-Field Holography Beyond the Assumption of Weak Objects"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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  • 2020Journal Article Research Paper
    [["dc.bibliographiccitation.artnumber","013501"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Medical Imaging"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Robisch, Anna Lena"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Meer, Franziska van der"],["dc.contributor.author","Stadelmann-Nessler, Christine"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-03-04T13:21:36Z"],["dc.date.available","2020-03-04T13:21:36Z"],["dc.date.issued","2020"],["dc.description.abstract","X-ray cone-beam holotomography of unstained tissue from the human central nervous system reveals details down to subcellular length scales. This visualization of variations in the electron density of the sample is based on phase-contrast techniques using intensities formed by self-interference of the beam between object and detector. Phase retrieval inverts diffraction and overcomes the phase problem by constraints such as several measurements at different Fresnel numbers for a single projection. Therefore, the object-to-detector distance (defocus) can be varied. However, for cone-beam geometry, changing defocus changes magnification, which can be problematic in view of image processing and resolution. Alternatively, the photon energy can be altered (multi-E). Far from absorption edges, multi-E data yield the wavelength-independent electron density. We present the multi-E holotomography at the Göttingen Instrument for Nano-Imaging with X-Rays (GINIX) setup of the P10 beamline at Deutsches Elektronen-Synchrotron. The instrument is based on a combined optics of elliptical mirrors and an x-ray waveguide positioned in the focal plane for further coherence, spatial filtering, and high numerical aperture. Previous results showed the suitability of this instrument for nanoscale tomography of unstained brain tissue. We demonstrate that upon energy variation, the focal spot is stable enough for imaging. To this end, a double-crystal monochromator and automated alignment routines are required. Three tomograms of human brain tissue were recorded and jointly analyzed using phase retrieval based on the contrast transfer function formalism generalized to multiple photon energies. Variations of the electron density of the sample are successfully reconstructed."],["dc.identifier.doi","10.1117/1.JMI.7.1.013501"],["dc.identifier.pmid","32016134"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63102"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/20"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","2329-4302"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Stadelmann-Nessler"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Nanoscale x-ray holotomography of human brain tissue with phase retrieval based on multienergy recordings"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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