Töpperwien, Mareike

[["dc.bibliographiccitation.firstpage","4331"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Biomedical Optics Express"],["dc.bibliographiccitation.lastpage","4347"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Carboni, Eleonora"],["dc.contributor.author","Nicolas, Jan-David"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Stadelmann-Nessler, Christine"],["dc.contributor.author","Lingor, Paul"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-11-09T09:25:21Z"],["dc.date.accessioned","2021-10-11T11:31:15Z"],["dc.date.available","2017-11-09T09:25:21Z"],["dc.date.available","2021-10-11T11:31:15Z"],["dc.date.issued","2017"],["dc.description.abstract","We have used scanning X-ray diffraction (XRD) and X-ray fluorescence (XRF) with micro-focused synchrotron radiation to study histological sections from human substantia nigra (SN). Both XRF and XRD mappings visualize tissue properties, which are inaccessible by conventional microscopy and histology. We propose to use these advanced tools to characterize neuronal tissue in neurodegeneration, in particular in Parkinson's disease (PD). To this end, we take advantage of the recent experimental progress in x-ray focusing, detection, and use automated data analysis scripts to enable quantitative analysis of large field of views. XRD signals are recorded and analyzed both in the regime of small-angle (SAXS) and wide-angle x-ray scattering (WAXS). The SAXS signal was analyzed in view of the local myelin structure, while WAXS was used to identify crystalline deposits. PD tissue scans exhibited increased amounts of crystallized cholesterol. The XRF analysis showed increased amounts of iron and decreased amounts of copper in the PD tissue compared to the control."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.1364/BOE.8.004331"],["dc.identifier.gro","3142465"],["dc.identifier.pmid","29082068"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14826"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/90600"],["dc.language","eng"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.issn","2156-7085"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","Goescholar"],["dc.rights.access","openAccess"],["dc.rights.uri","https://goedoc.uni-goettingen.de/licenses"],["dc.subject","170.6510) Spectroscopy, tissue diagnostics; (170.6935) Tissue characterization; (180.5810) Scanning microscopy; (180.7460) X-ray microscopy"],["dc.subject.ddc","530"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Imaging of neuronal tissues by x-ray diffraction and x-ray fluorescence microscopy: evaluation of contrast and biomarkers for neurodegenerative diseases"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","383"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.lastpage","392"],["dc.bibliographiccitation.volume","45"],["dc.contributor.author","Töpperwien, M."],["dc.contributor.author","Priebe, M."],["dc.contributor.author","Salditt, T."],["dc.date.accessioned","2017-09-07T11:44:50Z"],["dc.date.available","2017-09-07T11:44:50Z"],["dc.date.issued","2016"],["dc.description.abstract","We have performed scanning nano-beam small-angle X-ray scattering (nano-SAXS) experiments on in vitro-formed actin filaments cross-linked with -actinin. The experimental method combines a high resolution in reciprocal space with a real space resolution as given by the spot-size of the nano-focused X-ray beam, and opens up new opportunities to study local super-molecular structures of actin filaments. In this first proof-of-concept, we show that the local orientation of actin bundles formed by the cross-linking can be visualized by the X-ray darkfield maps. The filament bundles give rise to highly anisotropic diffraction patterns showing distinct streaks perpendicular to the bundle axes. Interestingly, some diffraction patterns exhibit a fine structure in the form of intensity modulations allowing for a more detailed analysis of the order within the bundles. A first empirical quantification of these modulations is included in the present work."],["dc.identifier.doi","10.1007/s00249-015-1107-9"],["dc.identifier.gro","3141659"],["dc.identifier.isi","000378049100001"],["dc.identifier.pmid","26715112"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/6342"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft [SFB 937/A11]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray scattering"],["dc.title","Actin bundles cross-linked with α -actinin studied by nanobeam X-ray diffraction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","518"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","529"],["dc.bibliographiccitation.volume","28"],["dc.contributor.affiliation","Wittmeier, Andrew; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Cassini, Chiara; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Töpperwien, Mareike; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Denz, Manuela; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Hagemann, Johannes; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Osterhoff, Markus; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Salditt, Tim; 1Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.author","Wittmeier, Andrew"],["dc.contributor.author","Cassini, Chiara"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Denz, Manuela"],["dc.contributor.author","Hagemann, Johannes"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2021-04-14T08:28:23Z"],["dc.date.available","2021-04-14T08:28:23Z"],["dc.date.issued","2021"],["dc.date.updated","2022-02-09T13:21:31Z"],["dc.description.abstract","X‐rays are emerging as a complementary probe to visible‐light photons and electrons for imaging biological cells. By exploiting their small wavelength and high penetration depth, it is possible to image whole, intact cells and resolve subcellular structures at nanometer resolution. A variety of X‐ray methods for cell imaging have been devised for probing different properties of biological matter, opening up various opportunities for fully exploiting different views of the same sample. Here, a combined approach is employed to study cell nuclei of NIH‐3T3 fibroblasts. Scanning small‐angle X‐ray scattering is combined with X‐ray holography to quantify length scales, aggregation state, and projected electron and mass densities of the nuclear material. Only by joining all this information is it possible to spatially localize nucleoli, heterochromatin and euchromatin, and physically characterize them. It is thus shown that for complex biological systems, like the cell nucleus, combined imaging approaches are highly valuable."],["dc.description.abstract","The combination of small‐angle X‐ray scattering and X‐ray holography enables us to visualize and characterize biological material in cell nuclei spanning multiple length scales. image"],["dc.identifier.doi","10.1107/S1600577520016276"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82591"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/218"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","International Union of Crystallography"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1600-5775"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use,\r\n distribution and reproduction in any medium, provided the original work is properly cited."],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cellular biophysics"],["dc.title","Combined scanning small-angle X-ray scattering and holography probes multiple length scales in cell nuclei"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]