Schnorrenberg, Sebastian

[["dc.bibliographiccitation.firstpage","15655"],["dc.bibliographiccitation.issue","49"],["dc.bibliographiccitation.journal","Angewandte Chemie"],["dc.bibliographiccitation.lastpage","15659"],["dc.bibliographiccitation.volume","128"],["dc.contributor.author","Roubinet, Benoît"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Alt, Philipp"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Shojaei, Heydar"],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Nizamov, Shamil"],["dc.contributor.author","Irie, Masahiro"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-04-23T11:48:24Z"],["dc.date.available","2018-04-23T11:48:24Z"],["dc.date.issued","2016"],["dc.description.abstract","Reversibel photoschaltbares 1,2‐Bis(2‐ethyl‐6‐phenyl‐1‐benzothiophen‐1,1‐dioxid‐3‐yl)perfluorcyclopenten (EBT) mit fluoreszierender “geschlossener” Form wurde mit vier oder acht Carboxygruppen versehen und an Antikörper gebunden. Die carboxylierten Derivate wiesen geringe Aggregation, effizientes Photoschalten in wässrigen Puffern, gezieltes Färben von zellulären Strukturen und gute photophysikalische Eigenschaften auf. Abwechselnde Bestrahlung mit UV und blauem Licht relativ geringer Intensität führte zu reversibler photochemischer Isomerisierung zwischen zwei stabilen Strukturen über mehrere dutzend Schaltzyklen. Dies ermöglichte die Verwendung der Farbstoffe für hochauflösende RESOLFT‐Mikroskopie (“reversible switchable optical linear fluorescence transitions”). Hierbei konnte eine optische Auflösung von 75 nm an zellulären Tubulin‐Filamenten erzielt werden."],["dc.identifier.doi","10.1002/ange.201607940"],["dc.identifier.gro","3142364"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13503"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","0044-8249"],["dc.title","Carboxylierte photoschaltbare Diarylethene als Biomarkierungen für hochauflösende RESOLFT-Mikroskopie"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","15429"],["dc.bibliographiccitation.issue","49"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","15433"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Roubinet, Benoît"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Alt, Philipp"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Shojaei, Heydar"],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Nizamov, Shamil"],["dc.contributor.author","Irie, Masahiro"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:53:03Z"],["dc.date.available","2017-09-07T11:53:03Z"],["dc.date.issued","2016"],["dc.description.abstract","Reversibly photoswitchable 1,2-bis(2-ethyl-6-phenyl-1-benzothiophene-1,1-dioxide-3-yl)perfluorocyclopentenes (EBT) having fluorescent “closed” forms were decorated with four or eight carboxylic groups and attached to antibodies. Low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures, and good photophysical properties were demonstrated. Alternating light pulses of UV and blue light induce numerous reversible photochemical transformations between two stables states with distinct structures. Using relatively low light intensities, EBTs were applied in biology-related super-resolution microscopy based on the reversible saturable (switchable) optical linear fluorescence transitions (RESOLFT) and demonstrated optical resolution of 75 nm."],["dc.identifier.doi","10.1002/anie.201607940"],["dc.identifier.gro","3145016"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2706"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","1433-7851"],["dc.title","Carboxylated Photoswitchable Diarylethenes for Biolabeling and Super-Resolution RESOLFT Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3324"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Chemical Science"],["dc.bibliographiccitation.lastpage","3334"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Lukinavičius, Gražvydas"],["dc.contributor.author","Mitronova, Gyuzel Y."],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Barthel, Hannah"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:46:09Z"],["dc.date.available","2022-03-01T11:46:09Z"],["dc.date.issued","2018"],["dc.description.abstract","Nanoscopy compatible fluorescent tubulin probes can be used to stain microtubules and chitin-rich taenidia in the insect tracheoles."],["dc.description.abstract","We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents – taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly ( Drosophila melanogaster ) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae."],["dc.description.abstract","Nanoscopy compatible fluorescent tubulin probes can be used to stain microtubules and chitin-rich taenidia in the insect tracheoles."],["dc.description.abstract","We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents – taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly ( Drosophila melanogaster ) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae."],["dc.identifier.doi","10.1039/C7SC05334G"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103579"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","2041-6539"],["dc.relation.issn","2041-6520"],["dc.rights.uri","http://creativecommons.org/licenses/by-nc/3.0/"],["dc.title","Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]