Schnorrenberg, Sebastian

[["dc.bibliographiccitation.firstpage","15655"],["dc.bibliographiccitation.issue","49"],["dc.bibliographiccitation.journal","Angewandte Chemie"],["dc.bibliographiccitation.lastpage","15659"],["dc.bibliographiccitation.volume","128"],["dc.contributor.author","Roubinet, Benoît"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Alt, Philipp"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Shojaei, Heydar"],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Nizamov, Shamil"],["dc.contributor.author","Irie, Masahiro"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-04-23T11:48:24Z"],["dc.date.available","2018-04-23T11:48:24Z"],["dc.date.issued","2016"],["dc.description.abstract","Reversibel photoschaltbares 1,2‐Bis(2‐ethyl‐6‐phenyl‐1‐benzothiophen‐1,1‐dioxid‐3‐yl)perfluorcyclopenten (EBT) mit fluoreszierender “geschlossener” Form wurde mit vier oder acht Carboxygruppen versehen und an Antikörper gebunden. Die carboxylierten Derivate wiesen geringe Aggregation, effizientes Photoschalten in wässrigen Puffern, gezieltes Färben von zellulären Strukturen und gute photophysikalische Eigenschaften auf. Abwechselnde Bestrahlung mit UV und blauem Licht relativ geringer Intensität führte zu reversibler photochemischer Isomerisierung zwischen zwei stabilen Strukturen über mehrere dutzend Schaltzyklen. Dies ermöglichte die Verwendung der Farbstoffe für hochauflösende RESOLFT‐Mikroskopie (“reversible switchable optical linear fluorescence transitions”). Hierbei konnte eine optische Auflösung von 75 nm an zellulären Tubulin‐Filamenten erzielt werden."],["dc.identifier.doi","10.1002/ange.201607940"],["dc.identifier.gro","3142364"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13503"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","0044-8249"],["dc.title","Carboxylierte photoschaltbare Diarylethene als Biomarkierungen für hochauflösende RESOLFT-Mikroskopie"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","15429"],["dc.bibliographiccitation.issue","49"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","15433"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Roubinet, Benoît"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Alt, Philipp"],["dc.contributor.author","Leutenegger, Marcel"],["dc.contributor.author","Shojaei, Heydar"],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Nizamov, Shamil"],["dc.contributor.author","Irie, Masahiro"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:53:03Z"],["dc.date.available","2017-09-07T11:53:03Z"],["dc.date.issued","2016"],["dc.description.abstract","Reversibly photoswitchable 1,2-bis(2-ethyl-6-phenyl-1-benzothiophene-1,1-dioxide-3-yl)perfluorocyclopentenes (EBT) having fluorescent “closed” forms were decorated with four or eight carboxylic groups and attached to antibodies. Low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures, and good photophysical properties were demonstrated. Alternating light pulses of UV and blue light induce numerous reversible photochemical transformations between two stables states with distinct structures. Using relatively low light intensities, EBTs were applied in biology-related super-resolution microscopy based on the reversible saturable (switchable) optical linear fluorescence transitions (RESOLFT) and demonstrated optical resolution of 75 nm."],["dc.identifier.doi","10.1002/anie.201607940"],["dc.identifier.gro","3145016"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2706"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","1433-7851"],["dc.title","Carboxylated Photoswitchable Diarylethenes for Biolabeling and Super-Resolution RESOLFT Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]