Schnorrenberg, Sebastian

[["dc.bibliographiccitation.artnumber","e15567"],["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Vorbrüggen, Gerd"],["dc.contributor.author","Herzig, Alf"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2020-12-10T18:48:05Z"],["dc.date.available","2020-12-10T18:48:05Z"],["dc.date.issued","2016"],["dc.description.abstract","Despite remarkable developments in diffraction unlimited super-resolution microscopy, in vivo nanoscopy of tissues and model organisms is still not satisfactorily established and rarely realized. RESOLFT nanoscopy is particularly suited for live cell imaging because it requires relatively low light levels to overcome the diffraction barrier. Previously, we introduced the reversibly switchable fluorescent protein rsEGFP2, which facilitated fast RESOLFT nanoscopy (Grodohann et al., 2012). In that study, as in most other nanoscopy studies, only cultivated single cells were analyzed. Here, we report on the use of rsEGFP2 for live-cell RESOLFT nanoscopy of sub-cellular structures of intact Drosophila melanogaster larvae and of resected tissues. We generated flies expressing fusion proteins of alpha-tubulin and rsEGFP2 highlighting the microtubule cytoskeleton in all cells. By focusing through the intact larval cuticle, we achieved lateral resolution of <60 nm. RESOLFT nanoscopy enabled time-lapse recordings comprising 40 images and facilitated recordings 40 ism deep within fly tissues."],["dc.identifier.doi","10.7554/eLife.15567"],["dc.identifier.eissn","2050-084X"],["dc.identifier.fs","626317"],["dc.identifier.gro","3141660"],["dc.identifier.isi","000379856900001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13549"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/79008"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2050-084X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","In vivo super-resolution RESOLFT microscopy of Drosophila melanogaster"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Plant Direct"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","Ghareeb, Hassan"],["dc.contributor.author","Frahm, Lars"],["dc.contributor.author","Grotjohann, Tim"],["dc.contributor.author","Jensen, Nickels"],["dc.contributor.author","Teichmann, Thomas"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lipka, Volker"],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2021-04-14T08:23:40Z"],["dc.date.available","2021-04-14T08:23:40Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract Subdiffraction super‐resolution fluorescence microscopy, or nanoscopy, has seen remarkable developments in the last two decades. Yet, for the visualization of plant cells, nanoscopy is still rarely used. In this study, we established RESOLFT nanoscopy on living green plant tissue. Live‐cell RESOLFT nanoscopy requires and utilizes comparatively low light doses and intensities to overcome the diffraction barrier. We generated a transgenic Arabidopsis thaliana plant line expressing the reversibly switchable fluorescent protein rsEGFP2 fused to the mammalian microtubule‐associated protein 4 (MAP4) in order to ubiquitously label the microtubule cytoskeleton. We demonstrate the use of RESOLFT nanoscopy for extended time‐lapse imaging of cortical microtubules in Arabidopsis leaf discs. By combining our approach with fluorescence lifetime gating, we were able to acquire live‐cell RESOLFT images even close to chloroplasts, which exhibit very strong autofluorescence. The data demonstrate the feasibility of subdiffraction resolution imaging in transgenic plant material with minimal requirements for sample preparation."],["dc.identifier.doi","10.1002/pld3.261"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81007"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2475-4455"],["dc.relation.issn","2475-4455"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],["dc.title","Live‐cell RESOLFT nanoscopy of transgenic Arabidopsis thaliana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","21956"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Frahm, Lars"],["dc.contributor.author","Keller-Findeisen, Jan"],["dc.contributor.author","Alt, Philipp"],["dc.contributor.author","Schnorrenberg, Sebastian"],["dc.contributor.author","del Álamo Ruiz, Miguel"],["dc.contributor.author","Aspelmeier, Timo"],["dc.contributor.author","Munk, Axel"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2020-12-10T18:42:02Z"],["dc.date.available","2020-12-10T18:42:02Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1364/OE.27.021956"],["dc.identifier.pmid","31510262"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16747"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77780"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/204"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","DOI-Import GROB-394"],["dc.notes.intern","Merged from goescholar"],["dc.relation","RTG 2088: Research Training Group 2088 Discovering structure in complex data: Statistics meets Optimization and Inverse Problems"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Hell"],["dc.relation.workinggroup","RG Jakobs (Structure and Dynamics of Mitochondria)"],["dc.relation.workinggroup","RG Munk"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Molecular contribution function in RESOLFT nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]