Reichard, Utz

[["dc.bibliographiccitation.firstpage","5517"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Journal of Proteome Research"],["dc.bibliographiccitation.lastpage","5529"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Singh, Bharat"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Kumar, Ram"],["dc.contributor.author","Kumar, Manish"],["dc.contributor.author","Bhadoria, Dharam P."],["dc.contributor.author","Reichard, Utz"],["dc.contributor.author","Gupta, Vijay K."],["dc.contributor.author","Sharma, Gainda L."],["dc.contributor.author","Asif, Abdul R."],["dc.date.accessioned","2018-11-07T08:37:39Z"],["dc.date.available","2018-11-07T08:37:39Z"],["dc.date.issued","2010"],["dc.description.abstract","The secreted proteomes of a three week old culture of an Indian (190/96) and a German (DAYA) Aspergillus fumigatus isolate were investigated for reactivity with IgG and/or IgE antibodies derived from pooled allergic broncho-pulmonary aspergillosis (ABPA) patients' sera. Two dimensional Western blotting followed by mass spectrometric analysis of the reactive protein spots revealed 35 proteins from the two A. fumigatus strains. There were seven known A. fumigatus allergens among them (Asp f1-4, Asp 19, Asp f10, and Asp f13/15), whereas three proteins displaying significant sequence similarity to known fungal allergens have been assigned as predicted allergens (Dipeptidyl-peptidase-V precursor, Nuclear transport factor 2, and Malate dehydrogenase, NAD-dependent). Eight IgG and IgE reactive proteins were common in both strains; however, 12 proteins specifically reacted in 190/96 and 15 in DAYA. Further testing with sera of 5 individual ABPA patients demonstrated that 12 out of 20 immunoreactive proteins of 190/96 strain of A. fumigatus had consistent reactivity with IgE. Seven of these proteins reacted with IgG also. The 25 of 35 identified proteins are novel with respect to immunoreactivity with ABPA patients' sera and could form a panel of molecules to improve the currently existing less-sensitive diagnostic methods. Through expressing recombinantly, these proteins may also serve as a tool in desensibilization strategies."],["dc.identifier.doi","10.1021/pr100604x"],["dc.identifier.isi","000283810500002"],["dc.identifier.pmid","20828163"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6075"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18588"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","1535-3893"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Immuno-Reactive Molecules Identified from the Secreted Proteome of Aspergillus fumigatus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5530"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Journal of Proteome Research"],["dc.bibliographiccitation.lastpage","5541"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Singh, Bharat"],["dc.contributor.author","Sharma, Gainda L."],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Kumar, Ram"],["dc.contributor.author","Singh, Seema"],["dc.contributor.author","Bhadoria, Dharam P."],["dc.contributor.author","Katyal, Anju"],["dc.contributor.author","Reichard, Utz"],["dc.contributor.author","Asif, Abdul R."],["dc.date.accessioned","2018-11-07T08:37:39Z"],["dc.date.available","2018-11-07T08:37:39Z"],["dc.date.issued","2010"],["dc.description.abstract","Aspergillus fumigatus is the common cause of allergic broncho-pulmonary aspergillosis (ABPA) and most of the allergens have been described from its secreted fraction. In the present investigation, germinating conidial cytosolic proteins of A. fumigatus were extracted from a 16 h culture. The proteome from this fraction was developed, and immuno-blots were generated using pooled ABPA patients' sera. Well separated Immunoglobulin-E (IgE) and Immunoglobulin-G (IgG) reactive spots were picked from corresponding 2DE gels and subjected to mass spectrometric analysis. As a result, 66 immuno-reactive proteins were identified from two geographically different strains (190/96 and DAYA) of A. fumigatus. Only 3 out of 66 proteins reacted with IgG, and the remaining 63 proteins were found to be IgE reactive. These 63 IgE-reactive cytosolic proteins from germinating conidia included 2 already known (Asp f12 and Asp f22) and 4 predicted allergens (Hsp88, Hsp70, malate dehydrogenase, and alcohol dehydrogenase) based on their homology with other known fungal allergens. In view of this, the panel of presently identified IgE-reactive novel proteins holds the potential of providing a basis for the wider diagnostic application in assay for allergic aspergillosis. We could demonstrate that recombinantly expressed proteins from this panel showed consistent reactivity with IgE of individual sera of ABPA patients. The recombinantly expressed proteins may also be useful in desensitization therapy of allergic disorders including ABPA."],["dc.identifier.doi","10.1021/pr100605c"],["dc.identifier.isi","000283810500003"],["dc.identifier.pmid","20828162"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18589"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","1535-3893"],["dc.title","Novel Cytosolic Allergens of Aspergillus fumigatus Identified from Germinating Conidia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]