Reichard, Utz

[["dc.bibliographiccitation.firstpage","424"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Current protein & peptide science"],["dc.bibliographiccitation.lastpage","429"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Reichard, Utz"],["dc.contributor.author","Herrmann, Sahra"],["dc.contributor.author","Asif, Abdul R."],["dc.date.accessioned","2019-07-09T11:41:29Z"],["dc.date.available","2019-07-09T11:41:29Z"],["dc.date.issued","2014"],["dc.description.abstract","Although innate immunity primarily combats systemic infections of opportunistic fungi such as Aspergillus and Candida spp., acquired and protective immunoreactions were observed long ago in animal trials following sublethal systemic infections caused by viable fungi or after challenging animals with inactivated fungal cells. Based on these observations, fungal antigens should exist which mediate such protective immunoreactions and have in part already been identified. In this context, this review focuses primarily on the various approaches that have been used to identify protection-mediating Aspergillus-antigens and their rationale. Emphasis is placed on screening methods that have exploited genetic or proteomic approaches on the basis of the corresponding fungal genome projects. Thereby, a survey and description is given of the antigens so far known to be capable of inducing immune responses that protect animals against acquiring lethal systemic aspergillosis."],["dc.identifier.doi","10.2174/1389203715666140512122037"],["dc.identifier.fs","611058"],["dc.identifier.pmid","24818757"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12124"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58444"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1875-5550"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Antigens, Fungal"],["dc.subject.mesh","Aspergillosis"],["dc.subject.mesh","Aspergillus fumigatus"],["dc.subject.mesh","Humans"],["dc.subject.mesh","Opportunistic Infections"],["dc.subject.mesh","Vaccination"],["dc.title","Vaccination approaches against opportunistic fungal infections caused by Aspergillus fumigatus."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","submitted_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5517"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Journal of Proteome Research"],["dc.bibliographiccitation.lastpage","5529"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Singh, Bharat"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Kumar, Ram"],["dc.contributor.author","Kumar, Manish"],["dc.contributor.author","Bhadoria, Dharam P."],["dc.contributor.author","Reichard, Utz"],["dc.contributor.author","Gupta, Vijay K."],["dc.contributor.author","Sharma, Gainda L."],["dc.contributor.author","Asif, Abdul R."],["dc.date.accessioned","2018-11-07T08:37:39Z"],["dc.date.available","2018-11-07T08:37:39Z"],["dc.date.issued","2010"],["dc.description.abstract","The secreted proteomes of a three week old culture of an Indian (190/96) and a German (DAYA) Aspergillus fumigatus isolate were investigated for reactivity with IgG and/or IgE antibodies derived from pooled allergic broncho-pulmonary aspergillosis (ABPA) patients' sera. Two dimensional Western blotting followed by mass spectrometric analysis of the reactive protein spots revealed 35 proteins from the two A. fumigatus strains. There were seven known A. fumigatus allergens among them (Asp f1-4, Asp 19, Asp f10, and Asp f13/15), whereas three proteins displaying significant sequence similarity to known fungal allergens have been assigned as predicted allergens (Dipeptidyl-peptidase-V precursor, Nuclear transport factor 2, and Malate dehydrogenase, NAD-dependent). Eight IgG and IgE reactive proteins were common in both strains; however, 12 proteins specifically reacted in 190/96 and 15 in DAYA. Further testing with sera of 5 individual ABPA patients demonstrated that 12 out of 20 immunoreactive proteins of 190/96 strain of A. fumigatus had consistent reactivity with IgE. Seven of these proteins reacted with IgG also. The 25 of 35 identified proteins are novel with respect to immunoreactivity with ABPA patients' sera and could form a panel of molecules to improve the currently existing less-sensitive diagnostic methods. Through expressing recombinantly, these proteins may also serve as a tool in desensibilization strategies."],["dc.identifier.doi","10.1021/pr100604x"],["dc.identifier.isi","000283810500002"],["dc.identifier.pmid","20828163"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6075"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18588"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","1535-3893"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Immuno-Reactive Molecules Identified from the Secreted Proteome of Aspergillus fumigatus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]