Rembold, Katja

[["dc.bibliographiccitation.firstpage","1858"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Ecology and Evolution"],["dc.bibliographiccitation.lastpage","1868"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Amandita, Fitri Y."],["dc.contributor.author","Rembold, Katja"],["dc.contributor.author","Vornam, Barbara"],["dc.contributor.author","Rahayu, Sri"],["dc.contributor.author","Siregar, Iskandar Z."],["dc.contributor.author","Kreft, Holger"],["dc.contributor.author","Finkeldey, Reiner"],["dc.date.accessioned","2019-07-09T11:50:07Z"],["dc.date.available","2019-07-09T11:50:07Z"],["dc.date.issued","2019"],["dc.description.abstract","The rapid conversion of Southeast Asian lowland rainforests into monocultures calls for the development of rapid methods for species identification to support ecological research and sustainable land-use management. Here, we investigated the utilization of DNA barcodes for identifying flowering plants from Sumatra, Indonesia. A total of 1,207 matK barcodes (441 species) and 2,376 rbcL barcodes (750 species) were successfully generated. The barcode effectiveness is assessed using four approaches: (a) comparison between morphological and molecular identification results, (b) best-close match analysis with TaxonDNA, (c) barcoding gap analysis, and (d) formation of monophyletic groups. Results show that rbcL has a much higher level of sequence recoverability than matK (95% and 66%). The comparison between morphological and molecular identifications revealed that matK and rbcL worked best assigning a plant specimen to the genus level. Estimates of identification success using best-close match analysis showed that >70% of the investigated species were correctly identified when using single barcode. The use of two-loci barcodes was able to increase the identification success up to 80%. The barcoding gap analysis revealed that neither matK nor rbcL succeeded to create a clear gap between the intraspecific and interspecific divergences. However, these two barcodes were able to discriminate at least 70% of the species from each other. Fifteen genera and twenty-one species were found to be nonmonophyletic with both markers. The two-loci barcodes were sufficient to reconstruct evolutionary relationships among the plant taxa in the study area that are congruent with the broadly accepted APG III phylogeny."],["dc.identifier.doi","10.1002/ece3.4875"],["dc.identifier.pmid","30847077"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15864"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59706"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation","SFB 990: Ökologische und sozioökonomische Funktionen tropischer Tieflandregenwald-Transformationssysteme (Sumatra, Indonesien)"],["dc.relation","SFB 990 | B | B06: Taxonomische, funktionelle, phylogenetische und biogeographische Diversität vaskulärer Pflanzen in Regenwald-Transformationssystemen auf Sumatra (Indonesien)"],["dc.relation","SFB 990 | Z | Z02: Central Scientific Support Unit"],["dc.relation.issn","2045-7758"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","630"],["dc.subject.gro","sfb990_journalarticles"],["dc.title","DNA barcoding of flowering plants in Sumatra, Indonesia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]