Jähne, Sebastian

[["dc.bibliographiccitation.firstpage","1083"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Analytical Atomic Spectrometry"],["dc.bibliographiccitation.lastpage","1087"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Kabatas, Selda"],["dc.contributor.author","Agüi-Gonzalez, Paola"],["dc.contributor.author","Hinrichs, Rena"],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Opazo, Felipe"],["dc.contributor.author","Diederichsen, Ulf"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Phan, Nhu T. N."],["dc.date.accessioned","2020-12-10T18:11:29Z"],["dc.date.available","2020-12-10T18:11:29Z"],["dc.date.issued","2019"],["dc.description.abstract","Molecular imaging of targeted large biomolecules has been restricted in SIMS due to the limited number of probes containing SIMSdetectable isotopes.We introduce here new 19F-containingmolecules that can be conjugated in a site-specificmanner to nanobodies able to recognize fluorescent proteins (FPs) or mouse immunoglobulins (Igs). In this work, we demonstrate that it is possible to use the 19F-nanobodies to reveal the location of several cellular proteins previously tagged with FPs or Igs. This enables specific bio-imaging in SIMS for a vast repertoire of biomolecules, offering new opportunities to study specific structural and functional molecular interactions in biological specimens."],["dc.identifier.doi","10.1039/C9JA00117D"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16452"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74028"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/24"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar not merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/16278 but duplicate"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | B01: Der Verteilung struktureller Lipide in synaptischen Membranen"],["dc.relation.eissn","1364-5544"],["dc.relation.issn","0267-9477"],["dc.relation.issn","1364-5544"],["dc.relation.workinggroup","RG Phan"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 3.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.subject.ddc","610"],["dc.title","Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Chemical Communications"],["dc.contributor.author","Kabatas Glowacki, Selda"],["dc.contributor.author","Agüi-Gonzalez, Paola"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Opazo Davila, Luis Felipe"],["dc.contributor.author","Phan, Nhu T. N."],["dc.contributor.author","Rizzoli, Silvio O."],["dc.date.accessioned","2022-07-01T07:34:55Z"],["dc.date.available","2022-07-01T07:34:55Z"],["dc.date.issued","2022"],["dc.description.abstract","We developed here an iodine-containing probe that can be used to identify the molecules of interest in secondary ion mass spectrometry (SIMS) by simple immunolabelling procedures. The immunolabelled iodine probe was readily combined with previously-developed SIMS probes carrying fluorine, to generate dual-channel SIMS data. This probe should provide a useful complement to the currently available SIMS probes, thus expanding the scope of this technology."],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft https://doi.org/10.13039/501100001659"],["dc.description.sponsorship"," Volkswagen Foundation https://doi.org/10.13039/501100001663"],["dc.identifier.doi","10.1039/D2CC02290G"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112039"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/501"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/167"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-581"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität"],["dc.relation.eissn","1364-548X"],["dc.relation.issn","1359-7345"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.relation.workinggroup","RG Phan"],["dc.rights","CC BY 3.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/3.0/"],["dc.title","An iodine-containing probe as a tool for molecular detection in secondary ion mass spectrometry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","The EMBO Journal"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Truckenbrodt, Sven"],["dc.contributor.author","Viplav, Abhiyan"],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Vogts, Angela"],["dc.contributor.author","Denker, Annette"],["dc.contributor.author","Wildhagen, Hanna"],["dc.contributor.author","Fornasiero, Eugenio F"],["dc.contributor.author","Rizzoli, Silvio O"],["dc.date.accessioned","2020-12-10T18:42:37Z"],["dc.date.available","2020-12-10T18:42:37Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.15252/embj.201798044"],["dc.identifier.pmid","29950309"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15710"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78022"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/34"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/38"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P09: Proteinsortierung in der Synapse: Prinzipien und molekulare Organisation"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1151"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Nature Neuroscience"],["dc.bibliographiccitation.lastpage","1162"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Helm, Martin S."],["dc.contributor.author","Dankovich, Tal M."],["dc.contributor.author","Mandad, Sunit"],["dc.contributor.author","Rammner, Burkhard"],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Salimi, Vanessa"],["dc.contributor.author","Koerbs, Christina"],["dc.contributor.author","Leibrandt, Richard"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.date.accessioned","2021-08-12T07:44:59Z"],["dc.date.available","2021-08-12T07:44:59Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1038/s41593-021-00874-w"],["dc.identifier.pii","874"],["dc.identifier.pmid","34168338"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88345"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/312"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/148"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/125"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P09: Proteinsortierung in der Synapse: Prinzipien und molekulare Organisation"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität"],["dc.relation","SFB 1286 | A08: Die Rolle post-translational modifizierter Proteine in der synaptischen Übertragung"],["dc.relation.eissn","1546-1726"],["dc.relation.issn","1097-6256"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.relation.workinggroup","RG Urlaub (Bioanalytische Massenspektrometrie)"],["dc.title","A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","172"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Experimental Cell Research"],["dc.bibliographiccitation.lastpage","179"],["dc.bibliographiccitation.volume","335"],["dc.contributor.author","Jaehne, Sebastian"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Helm, Martin S."],["dc.date.accessioned","2017-09-07T11:43:42Z"],["dc.date.available","2017-09-07T11:43:42Z"],["dc.date.issued","2015"],["dc.description.abstract","The function of endosomes and of endosome-like structures in the presynaptic compartment is still controversial. This is in part due to the absence of a consensus on definitions and markers for these compartments. Synaptic endosomes are sometimes seen as stable organelles, permanently present in the synapse. Alternatively, they are seen as short-lived intermediates in synaptic vesicle recycling, arising from the endocytosis of large vesicles from the plasma membrane, or from homotypic fusion of small vesicles. In addition, the potential function of the endosome is largely unknown in the synapse. Some groups have proposed that the endosome is involved in the sorting of synaptic vesicle proteins, albeit others have produced data that deny this possibility. In this review, we present the existing evidence for synaptic endosomes, we discuss their potential functions, and we highlight frequent technical pitfalls in the analysis of this elusive compartment. We also sketch a roadmap to definitely determine the role of synaptic endosomes for the synaptic vesicle cycle. Finally, we propose a common definition of synaptic endosome-like structures. (C) 2015 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.yexcr.2015.04.017"],["dc.identifier.gro","3141863"],["dc.identifier.isi","000357440900005"],["dc.identifier.pmid","25939282"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1912"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1090-2422"],["dc.relation.issn","0014-4827"],["dc.title","The structure and function of presynaptic endosomes"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","108841"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Cell Reports"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Mikulasch, Fabian"],["dc.contributor.author","Heuer, Helge G.H."],["dc.contributor.author","Truckenbrodt, Sven"],["dc.contributor.author","Agüi-Gonzalez, Paola"],["dc.contributor.author","Grewe, Katharina"],["dc.contributor.author","Vogts, Angela"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Priesemann, Viola"],["dc.date.accessioned","2021-04-14T08:29:03Z"],["dc.date.available","2021-04-14T08:29:03Z"],["dc.date.issued","2021"],["dc.description.abstract","Synaptic transmission relies on the continual exocytosis and recycling of synaptic vesicles. Aged vesicle proteins are prevented from recycling and are eventually degraded. This implies that active synapses would lose vesicles and vesicle-associated proteins over time, unless the supply correlates to activity, to balance the losses. To test this hypothesis, we first model the quantitative relation between presynaptic spike rate and vesicle turnover. The model predicts that the vesicle supply needs to increase with the spike rate. To follow up this prediction, we measure protein turnover in individual synapses of cultured hippocampal neurons by combining nanoscale secondary ion mass spectrometry (nanoSIMS) and fluorescence microscopy. We find that turnover correlates with activity at the single-synapse level, but not with other parameters such as the abundance of synaptic vesicles or postsynaptic density proteins. We therefore suggest that the supply of newly synthesized proteins to synapses is closely connected to synaptic activity."],["dc.identifier.doi","10.1016/j.celrep.2021.108841"],["dc.identifier.pmid","33730575"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82779"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/244"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/117"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität"],["dc.relation.issn","2211-1247"],["dc.relation.workinggroup","RG Priesemann (Physics, Complex Systems & Neural Networks)"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","Presynaptic activity and protein turnover are correlated at the single-synapse level"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","020901"],["dc.bibliographiccitation.firstpage","020901"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Neurophotonics"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Richter, Katharina N."],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Vogts, Angela"],["dc.contributor.author","Lovric, Jelena"],["dc.date.accessioned","2020-12-10T18:36:36Z"],["dc.date.available","2020-12-10T18:36:36Z"],["dc.date.issued","2017"],["dc.description.abstract","Investigating the detailed substructure of the cell is beyond the ability of conventional optical microscopy. Electron microscopy, therefore, has been the only option for such studies for several decades. The recent implementation of several super-resolution optical microscopy techniques has rendered the investigation of cellular substructure easier and more efficient. Nevertheless, optical microscopy only provides an image of the present structure of the cell, without any information on its long-temporal changes. These can be investigated by combining super-resolution optics with a nonoptical imaging technique, nanoscale secondary ion mass spectrometry, which investigates the isotopic composition of the samples. The resulting technique, combined isotopic and optical nanoscopy, enables the investigation of both the structure and the \"history\" of the cellular elements. The age and the turnover of cellular organelles can be read by isotopic imaging, while the structure can be analyzed by optical (fluorescence) approaches. We present these technologies, and we discuss their implementation for the study of biological samples. We conclude that, albeit complex, this type of technology is reliable enough for mass application to cell biology."],["dc.identifier.doi","10.1117/1.NPh.4.2.020901"],["dc.identifier.issn","2329-423X"],["dc.identifier.pmid","28466025"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14911"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76685"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.rights","CC BY-NC-SA 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-sa/3.0"],["dc.title","Review of combined isotopic and optical nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]