Jähne, Sebastian

[["dc.bibliographiccitation.firstpage","1083"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Analytical Atomic Spectrometry"],["dc.bibliographiccitation.lastpage","1087"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Kabatas, Selda"],["dc.contributor.author","Agüi-Gonzalez, Paola"],["dc.contributor.author","Hinrichs, Rena"],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Opazo, Felipe"],["dc.contributor.author","Diederichsen, Ulf"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Phan, Nhu T. N."],["dc.date.accessioned","2020-12-10T18:11:29Z"],["dc.date.available","2020-12-10T18:11:29Z"],["dc.date.issued","2019"],["dc.description.abstract","Molecular imaging of targeted large biomolecules has been restricted in SIMS due to the limited number of probes containing SIMSdetectable isotopes.We introduce here new 19F-containingmolecules that can be conjugated in a site-specificmanner to nanobodies able to recognize fluorescent proteins (FPs) or mouse immunoglobulins (Igs). In this work, we demonstrate that it is possible to use the 19F-nanobodies to reveal the location of several cellular proteins previously tagged with FPs or Igs. This enables specific bio-imaging in SIMS for a vast repertoire of biomolecules, offering new opportunities to study specific structural and functional molecular interactions in biological specimens."],["dc.identifier.doi","10.1039/C9JA00117D"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16452"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74028"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/24"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar not merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/16278 but duplicate"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | B01: Der Verteilung struktureller Lipide in synaptischen Membranen"],["dc.relation.eissn","1364-5544"],["dc.relation.issn","0267-9477"],["dc.relation.issn","1364-5544"],["dc.relation.workinggroup","RG Phan"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 3.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.subject.ddc","610"],["dc.title","Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Chemical Communications"],["dc.contributor.author","Kabatas Glowacki, Selda"],["dc.contributor.author","Agüi-Gonzalez, Paola"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Opazo Davila, Luis Felipe"],["dc.contributor.author","Phan, Nhu T. N."],["dc.contributor.author","Rizzoli, Silvio O."],["dc.date.accessioned","2022-07-01T07:34:55Z"],["dc.date.available","2022-07-01T07:34:55Z"],["dc.date.issued","2022"],["dc.description.abstract","We developed here an iodine-containing probe that can be used to identify the molecules of interest in secondary ion mass spectrometry (SIMS) by simple immunolabelling procedures. The immunolabelled iodine probe was readily combined with previously-developed SIMS probes carrying fluorine, to generate dual-channel SIMS data. This probe should provide a useful complement to the currently available SIMS probes, thus expanding the scope of this technology."],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft https://doi.org/10.13039/501100001659"],["dc.description.sponsorship"," Volkswagen Foundation https://doi.org/10.13039/501100001663"],["dc.identifier.doi","10.1039/D2CC02290G"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112039"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/501"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/167"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-581"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität"],["dc.relation.eissn","1364-548X"],["dc.relation.issn","1359-7345"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.relation.workinggroup","RG Phan"],["dc.rights","CC BY 3.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/3.0/"],["dc.title","An iodine-containing probe as a tool for molecular detection in secondary ion mass spectrometry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1355"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Analytical Atomic Spectrometry"],["dc.bibliographiccitation.lastpage","1368"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Agüi-Gonzalez, Paola"],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Phan, Nhu T. N."],["dc.date.accessioned","2020-12-10T18:11:29Z"],["dc.date.available","2020-12-10T18:11:29Z"],["dc.date.issued","2019"],["dc.description.abstract","Secondary ion mass spectrometry (SIMS) has been increasingly recognized as a powerful technique for visualizing molecular architectures in the fields of neurobiology and cell biology. There are two main platforms of SIMS, namely ToF-SIMS and nanoscale SIMS (nanoSIMS), which are capable of imaging different types of biomolecules with resolution at the single cell and organelle level, respectively. In this review, we focus on the fundamental aspects of SIMS, as well as on the current ongoing instrumental developments of this technology. Selective applications of SIMS in neurobiological and cell biological research are provided to demonstrate its strengths, limitations, and future potential in the field. We add several examples of correlative imaging techniques that combine SIMS with other technologies, while highlighting the current trend for comprehensive and specific bio-imaging."],["dc.identifier.doi","10.1039/C9JA00118B"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74029"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/14"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | B01: Der Verteilung struktureller Lipide in synaptischen Membranen"],["dc.relation.workinggroup","RG Phan"],["dc.rights","CC BY 3.0"],["dc.title","SIMS imaging in neurobiology and cell biology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","overview_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","The EMBO Journal"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Truckenbrodt, Sven"],["dc.contributor.author","Viplav, Abhiyan"],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Vogts, Angela"],["dc.contributor.author","Denker, Annette"],["dc.contributor.author","Wildhagen, Hanna"],["dc.contributor.author","Fornasiero, Eugenio F"],["dc.contributor.author","Rizzoli, Silvio O"],["dc.date.accessioned","2020-12-10T18:42:37Z"],["dc.date.available","2020-12-10T18:42:37Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.15252/embj.201798044"],["dc.identifier.pmid","29950309"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15710"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78022"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/34"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/38"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P09: Proteinsortierung in der Synapse: Prinzipien und molekulare Organisation"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","108841"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Cell Reports"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Mikulasch, Fabian"],["dc.contributor.author","Heuer, Helge G.H."],["dc.contributor.author","Truckenbrodt, Sven"],["dc.contributor.author","Agüi-Gonzalez, Paola"],["dc.contributor.author","Grewe, Katharina"],["dc.contributor.author","Vogts, Angela"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Priesemann, Viola"],["dc.date.accessioned","2021-04-14T08:29:03Z"],["dc.date.available","2021-04-14T08:29:03Z"],["dc.date.issued","2021"],["dc.description.abstract","Synaptic transmission relies on the continual exocytosis and recycling of synaptic vesicles. Aged vesicle proteins are prevented from recycling and are eventually degraded. This implies that active synapses would lose vesicles and vesicle-associated proteins over time, unless the supply correlates to activity, to balance the losses. To test this hypothesis, we first model the quantitative relation between presynaptic spike rate and vesicle turnover. The model predicts that the vesicle supply needs to increase with the spike rate. To follow up this prediction, we measure protein turnover in individual synapses of cultured hippocampal neurons by combining nanoscale secondary ion mass spectrometry (nanoSIMS) and fluorescence microscopy. We find that turnover correlates with activity at the single-synapse level, but not with other parameters such as the abundance of synaptic vesicles or postsynaptic density proteins. We therefore suggest that the supply of newly synthesized proteins to synapses is closely connected to synaptic activity."],["dc.identifier.doi","10.1016/j.celrep.2021.108841"],["dc.identifier.pmid","33730575"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82779"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/244"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/117"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität"],["dc.relation.issn","2211-1247"],["dc.relation.workinggroup","RG Priesemann (Physics, Complex Systems & Neural Networks)"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","Presynaptic activity and protein turnover are correlated at the single-synapse level"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","020901"],["dc.bibliographiccitation.firstpage","020901"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Neurophotonics"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Richter, Katharina N."],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Vogts, Angela"],["dc.contributor.author","Lovric, Jelena"],["dc.date.accessioned","2020-12-10T18:36:36Z"],["dc.date.available","2020-12-10T18:36:36Z"],["dc.date.issued","2017"],["dc.description.abstract","Investigating the detailed substructure of the cell is beyond the ability of conventional optical microscopy. Electron microscopy, therefore, has been the only option for such studies for several decades. The recent implementation of several super-resolution optical microscopy techniques has rendered the investigation of cellular substructure easier and more efficient. Nevertheless, optical microscopy only provides an image of the present structure of the cell, without any information on its long-temporal changes. These can be investigated by combining super-resolution optics with a nonoptical imaging technique, nanoscale secondary ion mass spectrometry, which investigates the isotopic composition of the samples. The resulting technique, combined isotopic and optical nanoscopy, enables the investigation of both the structure and the \"history\" of the cellular elements. The age and the turnover of cellular organelles can be read by isotopic imaging, while the structure can be analyzed by optical (fluorescence) approaches. We present these technologies, and we discuss their implementation for the study of biological samples. We conclude that, albeit complex, this type of technology is reliable enough for mass application to cell biology."],["dc.identifier.doi","10.1117/1.NPh.4.2.020901"],["dc.identifier.issn","2329-423X"],["dc.identifier.pmid","28466025"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14911"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76685"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.rights","CC BY-NC-SA 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-sa/3.0"],["dc.title","Review of combined isotopic and optical nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]