Höbartner, Claudia

[["dc.bibliographiccitation.firstpage","3172"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Chemical Science"],["dc.bibliographiccitation.lastpage","3180"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Halbmair, Karin"],["dc.contributor.author","Seikowski, Jan"],["dc.contributor.author","Tkach, Igor"],["dc.contributor.author","Höbartner, Claudia"],["dc.contributor.author","Sezer, Deniz"],["dc.contributor.author","Bennati, Marina"],["dc.date.accessioned","2016-07-05T10:41:32Z"],["dc.date.accessioned","2021-10-27T13:12:28Z"],["dc.date.available","2016-07-05T10:41:32Z"],["dc.date.available","2021-10-27T13:12:28Z"],["dc.date.issued","2016"],["dc.description.abstract","Structural information at atomic resolution of biomolecular assemblies, such as RNA and RNA protein complexes, is fundamental to comprehend biological function. Modern spectroscopic methods offer exceptional opportunities in this direction. Here we present the capability of pulse EPR to report highresolution long-range distances in RNAs by means of a recently developed spin labeled nucleotide, which carries the TEMPO group directly attached to the nucleobase and preserves Watson–Crick base-pairing. In a representative RNA duplex with spin-label separations up to 28 base pairs (z8 nm) we demonstrate that the label allows for a model-free conversion of inter-spin distances into base-pair separation (Dbp) if broadband pulse excitation at Q band frequencies (34 GHz) is applied. The observed distance distribution increases from 0.2 nm for Dbp ¼ 10 to only 0.5 nm for Dbp ¼ 28, consistent with only small deviations from the “ideal” A-form RNA structure. Molecular dynamics (MD) simulations conducted at 20 C show restricted conformational freedom of the label. MD-generated structural deviations from an “ideal” A-RNA geometry help disentangle the contributions of local flexibility of the label and its neighboring nucleobases and global deformations of the RNA double helix to the experimental distance distributions. The study demonstrates that our simple but strategic spin labeling procedure can access detailed structural information on RNAs at atomic resolution over distances that match the size of macromolecular RNA complexes."],["dc.description.sponsorship","DFG Collaborative Research Centre (CRC) [803]; Max Planck Society"],["dc.identifier.doi","10.1039/C5SC04631A"],["dc.identifier.isi","000374859300027"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13415"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91693"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc Chemistry"],["dc.relation.issn","2041-6539"],["dc.relation.issn","2041-6520"],["dc.relation.orgunit","Fakultät für Chemie"],["dc.rights","CC BY-NC 3.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/3.0"],["dc.subject","long-range distances; RNA; EPR spectroscopy"],["dc.title","High-resolution measurement of long-range distances in RNA: pulse EPR spectroscopy with TEMPO-labeled nucleotides"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3433"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Physical Chemistry Chemical Physics"],["dc.bibliographiccitation.lastpage","3437"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Tkach, Igor"],["dc.contributor.author","Pornsuwan, Soraya"],["dc.contributor.author","Höbartner, Claudia"],["dc.contributor.author","Wachowius, Falk"],["dc.contributor.author","Sigurdsson, Snorri Th."],["dc.contributor.author","Baranova, Tatiana Y."],["dc.contributor.author","Diederichsen, Ulf"],["dc.contributor.author","Sicoli, Giuseppe"],["dc.contributor.author","Bennati, Marina"],["dc.date.accessioned","2018-11-07T09:30:21Z"],["dc.date.available","2018-11-07T09:30:21Z"],["dc.date.issued","2013"],["dc.description.abstract","Pulsed electron-electron double resonance (PELDOR, also known as DEER) has become a method of choice to measure distances in biomolecules. In this work we show how the performance of the method can be improved at high EPR frequencies (94 GHz) using variable dual frequency irradiation in a dual mode cavity in order to obtain enhanced resolution toward orientation selection. Dipolar evolution traces of a representative RNA duplex and an alpha-helical peptide were analysed in terms of possible bi-radical structures by considering the inherent ambiguity of symmetry-related solutions."],["dc.identifier.doi","10.1039/c3cp44415e"],["dc.identifier.isi","000314846600006"],["dc.identifier.pmid","23381580"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10192"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31286"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc Chemistry"],["dc.relation.issn","1463-9076"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","341"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Magnetic Resonance"],["dc.bibliographiccitation.lastpage","346"],["dc.bibliographiccitation.volume","209"],["dc.contributor.author","Tkach, Igor"],["dc.contributor.author","Sicoli, Giuseppe"],["dc.contributor.author","Höbartner, Claudia"],["dc.contributor.author","Bennati, Marina"],["dc.date.accessioned","2021-03-05T08:58:08Z"],["dc.date.available","2021-03-05T08:58:08Z"],["dc.date.issued","2011"],["dc.identifier.doi","10.1016/j.jmr.2011.01.012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80020"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.issn","1090-7807"],["dc.title","A dual-mode microwave resonator for double electron–electron spin resonance spectroscopy at W-band microwave frequencies"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","969"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Applied Magnetic Resonance"],["dc.bibliographiccitation.lastpage","979"],["dc.bibliographiccitation.volume","45"],["dc.contributor.author","Tkach, Igor"],["dc.contributor.author","Halbmair, Karin"],["dc.contributor.author","Höbartner, Claudia"],["dc.contributor.author","Bennati, Marina"],["dc.date.accessioned","2018-11-07T09:34:02Z"],["dc.date.available","2018-11-07T09:34:02Z"],["dc.date.issued","2014"],["dc.description.abstract","Pulsed electron-electron double resonance (PELDOR/DEER) at high frequencies can provide information on the relative orientation of paramagnetic centres or spin labels, if those are rigidly oriented in a host biomolecule and experiments are performed with sufficient orientation selectivity. We present the first comparative PELDOR study at 263 and 94 GHz on a model RNA system containing rigid nitroxides. We show that at 263 GHz still considerable modulation depth is observed and orientation selectivity is significant, particularly in g (x)-g (y) plane of the nitroxides."],["dc.description.sponsorship","Max Planck Society; Deutsche Forschungsgemeinschaft (SFB 803)"],["dc.identifier.doi","10.1007/s00723-014-0581-z"],["dc.identifier.isi","000344058800002"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32095"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Wien"],["dc.relation.issn","1613-7507"],["dc.relation.issn","0937-9347"],["dc.title","High-frequency 263 GHz PELDOR"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","373"],["dc.bibliographiccitation.issue","58"],["dc.bibliographiccitation.journal","Angewandte Chemie. International Edition"],["dc.bibliographiccitation.lastpage","379"],["dc.bibliographiccitation.volume","59"],["dc.contributor.author","Meyer, Andreas"],["dc.contributor.author","Dechert, Sebastian"],["dc.contributor.author","Dey, Surjendu"],["dc.contributor.author","Höbartner, Claudia"],["dc.contributor.author","Bennati, Marina"],["dc.date.accessioned","2019-12-09T11:04:02Z"],["dc.date.accessioned","2021-10-27T13:12:49Z"],["dc.date.available","2019-12-09T11:04:02Z"],["dc.date.available","2021-10-27T13:12:49Z"],["dc.date.issued","2019"],["dc.description.abstract","Spectroscopic and biophysical methods for structural determination at atomic resolution are fundamental in studies of biological function. Here we introduce an approach to measure molecular distances in bio-macromolecules using 19 F nuclear spins and nitroxide radicals in combination with high-frequency (94 GHz/3.4 T) electron-nuclear double resonance (ENDOR). The small size and large gyromagnetic ratio of the 19 F label enables to access distances up to about 1.5 nm with an accuracy of 0.1-1 Å. The experiment is not limited by the size of the bio-macromolecule. Performance is illustrated on synthesized fluorinated model compounds as well as spin-labelled RNA duplexes. The results demonstrate that our simple but strategic spin-labelling procedure combined with state-of-the-art spectroscopy accesses a distance range crucial to elucidate active sites of nucleic acids or proteins in the solution state."],["dc.identifier.doi","10.1002/anie.201908584"],["dc.identifier.isbn","31539187"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16871"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91724"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.eissn","1521-3773"],["dc.relation.issn","1521-3773"],["dc.relation.orgunit","Fakultät für Chemie"],["dc.rights","CC BY-NC 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.subject","RNA; fluorine; high field ENDOR; spin labelling; structural biology"],["dc.subject.ddc","540"],["dc.title","Measurement of Angstrom to Nanometer Molecular Distances with 19 F Nuclear Spins by EPR/ENDOR Spectroscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6443"],["dc.bibliographiccitation.issue","36"],["dc.bibliographiccitation.journal","Angewandte Chemie. International Edition"],["dc.bibliographiccitation.lastpage","6447"],["dc.bibliographiccitation.volume","49"],["dc.contributor.author","Sicoli, Giuseppe"],["dc.contributor.author","Wachowius, Falk"],["dc.contributor.author","Bennati, Marina"],["dc.contributor.author","Höbartner, Claudia"],["dc.date.accessioned","2021-03-05T08:57:51Z"],["dc.date.available","2021-03-05T08:57:51Z"],["dc.date.issued","2010"],["dc.identifier.doi","10.1002/anie.201000713"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/79905"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation.issn","1433-7851"],["dc.title","Probing Secondary Structures of Spin-Labeled RNA by Pulsed EPR Spectroscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]